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Q9UEW8

- STK39_HUMAN

UniProt

Q9UEW8 - STK39_HUMAN

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Protein
STE20/SPS1-related proline-alanine-rich protein kinase
Gene
STK39, SPAK
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May act as a mediator of stress-activated signals.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921ATP By similarity
Active sitei192 – 1921Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 779ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine kinase activity Source: BHF-UCL
  4. receptor signaling protein serine/threonine kinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cellular hypotonic response Source: BHF-UCL
  2. intracellular signal transduction Source: BHF-UCL
  3. negative regulation of potassium ion transmembrane transport Source: BHF-UCL
  4. negative regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
  5. negative regulation of rubidium ion transmembrane transporter activity Source: BHF-UCL
  6. negative regulation of rubidium ion transport Source: BHF-UCL
  7. peptidyl-serine phosphorylation Source: BHF-UCL
  8. peptidyl-threonine phosphorylation Source: BHF-UCL
  9. positive regulation of potassium ion transport Source: Ensembl
  10. protein phosphorylation Source: UniProtKB
  11. regulation of inflammatory response Source: Ensembl
  12. response to stress Source: UniProtKB
  13. signal transduction by phosphorylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiQ9UEW8.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20/SPS1-related proline-alanine-rich protein kinase (EC:2.7.11.1)
Short name:
Ste-20-related kinase
Alternative name(s):
DCHT
Serine/threonine-protein kinase 39
Gene namesi
Name:STK39
Synonyms:SPAK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:17717. STK39.

Subcellular locationi

Cytoplasm Inferred. Nucleus Inferred
Note: Nucleus when caspase-cleaved Inferred.

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. basolateral plasma membrane Source: Ensembl
  3. cytoplasm Source: UniProtKB
  4. cytoskeleton Source: Ensembl
  5. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38243.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 545545STE20/SPS1-related proline-alanine-rich protein kinase
PRO_0000086722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei309 – 3091Phosphoserine; by PKC/PRKCQ1 Publication
Modified residuei349 – 3491N6-acetyllysine1 Publication
Modified residuei354 – 3541Phosphothreonine By similarity
Modified residuei370 – 3701Phosphoserine1 Publication
Modified residuei371 – 3711Phosphoserine1 Publication
Modified residuei385 – 3851Phosphoserine5 Publications

Post-translational modificationi

Phosphorylated at Ser-309 by PRKCQ.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9UEW8.
PaxDbiQ9UEW8.
PeptideAtlasiQ9UEW8.
PRIDEiQ9UEW8.

PTM databases

PhosphoSiteiQ9UEW8.

Expressioni

Tissue specificityi

Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.

Gene expression databases

BgeeiQ9UEW8.
CleanExiHS_STK39.
GenevestigatoriQ9UEW8.

Organism-specific databases

HPAiCAB046022.

Interactioni

Subunit structurei

The phosphorylated form forms a complex with WNK2 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CAB39Q9Y3764EBI-2680974,EBI-306905

Protein-protein interaction databases

BioGridi118159. 18 interactions.
IntActiQ9UEW8. 4 interactions.
MINTiMINT-6783158.
STRINGi9606.ENSP00000348278.

Structurei

3D structure databases

ProteinModelPortaliQ9UEW8.
SMRiQ9UEW8. Positions 53-397, 452-545.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 337275Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi360 – 3667Nuclear localization signal Reviewed prediction
Motifi387 – 3915Caspase cleavage related site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 5342Ala/Pro-rich
Add
BLAST

Domaini

PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000234204.
HOVERGENiHBG108518.
InParanoidiQ9UEW8.
KOiK08835.
OMAiLSVHDSQ.
OrthoDBiEOG715Q3W.
PhylomeDBiQ9UEW8.
TreeFamiTF105339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UEW8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAEPSGSPVH VQLPQQAAPV TAAAAAAPAA ATAAPAPAAP AAPAPAPAPA    50
AQAVGWPICR DAYELQEVIG SGATAVVQAA LCKPRQERVA IKRINLEKCQ 100
TSMDELLKEI QAMSQCSHPN VVTYYTSFVV KDELWLVMKL LSGGSMLDII 150
KYIVNRGEHK NGVLEEAIIA TILKEVLEGL DYLHRNGQIH RDLKAGNILL 200
GEDGSVQIAD FGVSAFLATG GDVTRNKVRK TFVGTPCWMA PEVMEQVRGY 250
DFKADMWSFG ITAIELATGA APYHKYPPMK VLMLTLQNDP PTLETGVEDK 300
EMMKKYGKSF RKLLSLCLQK DPSKRPTAAE LLKCKFFQKA KNREYLIEKL 350
LTRTPDIAQR AKKVRRVPGS SGHLHKTEDG DWEWSDDEMD EKSEEGKAAF 400
SQEKSRRVKE ENPEIAVSAS TIPEQIQSLS VHDSQGPPNA NEDYREASSC 450
AVNLVLRLRN SRKELNDIRF EFTPGRDTAD GVSQELFSAG LVDGHDVVIV 500
AANLQKIVDD PKALKTLTFK LASGCDGSEI PDEVKLIGFA QLSVS 545
Length:545
Mass (Da):59,474
Last modified:January 11, 2011 - v3
Checksum:i35E68F400A38A861
GO
Isoform 2 (identifier: Q9UEW8-2) [UniParc]FASTAAdd to Basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     28-46: Missing.

Show »
Length:526
Mass (Da):57,937
Checksum:iA1DD278DEE7A4334
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei28 – 4619Missing in isoform 2.
VSP_055889Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411A → AAP in AAC72238. 1 Publication
Sequence conflicti41 – 411A → AAP in AAD01901. 1 Publication
Sequence conflicti173 – 1731L → F in AAD01901. 1 Publication
Sequence conflicti416 – 4161A → V in AHW56588. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099989 mRNA. Translation: AAC72238.1.
KJ534948 mRNA. Translation: AHW56588.1.
AF030403 mRNA. Translation: AAD01901.1.
AC016723 Genomic DNA. Translation: AAY15003.1.
AC017069 Genomic DNA. Translation: AAY14897.1.
AC067940 Genomic DNA. Translation: AAY24032.1.
AF017635 mRNA. Translation: AAB70552.1.
CCDSiCCDS42770.1.
RefSeqiNP_037365.2. NM_013233.2.
UniGeneiHs.276271.

Genome annotation databases

EnsembliENST00000355999; ENSP00000348278; ENSG00000198648.
GeneIDi27347.
KEGGihsa:27347.
UCSCiuc002uea.3. human.

Polymorphism databases

DMDMi317373508.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099989 mRNA. Translation: AAC72238.1 .
KJ534948 mRNA. Translation: AHW56588.1 .
AF030403 mRNA. Translation: AAD01901.1 .
AC016723 Genomic DNA. Translation: AAY15003.1 .
AC017069 Genomic DNA. Translation: AAY14897.1 .
AC067940 Genomic DNA. Translation: AAY24032.1 .
AF017635 mRNA. Translation: AAB70552.1 .
CCDSi CCDS42770.1.
RefSeqi NP_037365.2. NM_013233.2.
UniGenei Hs.276271.

3D structure databases

ProteinModelPortali Q9UEW8.
SMRi Q9UEW8. Positions 53-397, 452-545.
ModBasei Search...

Protein-protein interaction databases

BioGridi 118159. 18 interactions.
IntActi Q9UEW8. 4 interactions.
MINTi MINT-6783158.
STRINGi 9606.ENSP00000348278.

Chemistry

BindingDBi Q9UEW8.
ChEMBLi CHEMBL1163108.
GuidetoPHARMACOLOGYi 2224.

PTM databases

PhosphoSitei Q9UEW8.

Polymorphism databases

DMDMi 317373508.

Proteomic databases

MaxQBi Q9UEW8.
PaxDbi Q9UEW8.
PeptideAtlasi Q9UEW8.
PRIDEi Q9UEW8.

Protocols and materials databases

DNASUi 27347.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000355999 ; ENSP00000348278 ; ENSG00000198648 .
GeneIDi 27347.
KEGGi hsa:27347.
UCSCi uc002uea.3. human.

Organism-specific databases

CTDi 27347.
GeneCardsi GC02M168774.
H-InvDB HIX0023910.
HGNCi HGNC:17717. STK39.
HPAi CAB046022.
MIMi 607648. gene.
neXtProti NX_Q9UEW8.
PharmGKBi PA38243.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000234204.
HOVERGENi HBG108518.
InParanoidi Q9UEW8.
KOi K08835.
OMAi LSVHDSQ.
OrthoDBi EOG715Q3W.
PhylomeDBi Q9UEW8.
TreeFami TF105339.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 2681.
SignaLinki Q9UEW8.

Miscellaneous databases

GeneWikii STK39.
GenomeRNAii 27347.
NextBioi 50438.
PROi Q9UEW8.
SOURCEi Search...

Gene expression databases

Bgeei Q9UEW8.
CleanExi HS_STK39.
Genevestigatori Q9UEW8.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SPAK, a STE20/SPS1-related kinase that activates the p38 pathway."
    Johnston A.M., Naselli G., Gonez L.J., Martin R.M., Harrison L.C., de Aizpurua H.J.
    Oncogene 19:4290-4297(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism."
    Corominas R., Yang X., Lin G.N., Kang S., Shen Y., Ghamsari L., Broly M., Rodriguez M., Tam S., Wanamaker S.A., Fan C., Yi S., Tasan M., Lemmens I., Kuang X., Zhao N., Malhotra D., Michaelson J.J.
    , Vacic V., Calderwood M.A., Roth F.P., Tavernier J., Horvath S., Salehi-Ashtiani K., Korkin D., Sebat J., Hill D.E., Hao T., Vidal M., Iakoucheva L.M.
    Nat. Commun. 5:3650-3650(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "New human member of the Ste20 family."
    Melnick M.B., Petitt M., Perrimon N., Comb M.J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Baytel D., Don J.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 279-545 (ISOFORM 1).
    Tissue: Testis.
  6. "SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway."
    Li Y., Hu J., Vita R., Sun B., Tabata H., Altman A.
    EMBO J. 23:1112-1122(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-309.
  7. "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
    Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S.
    Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Prostate cancer.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370; SER-371 AND SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTK39_HUMAN
AccessioniPrimary (citable) accession number: Q9UEW8
Secondary accession number(s): O14774
, Q53S90, Q53SL7, Q53SS1, Q9UER4, X5D9C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 11, 2011
Last modified: September 3, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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