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Protein

Vesicle transport through interaction with t-SNAREs homolog 1B

Gene

VTI1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

GO - Molecular functioni

  1. chloride channel inhibitor activity Source: UniProtKB
  2. SNAP receptor activity Source: GO_Central
  3. SNARE binding Source: MGI

GO - Biological processi

  1. cell proliferation Source: ProtInc
  2. ER to Golgi vesicle-mediated transport Source: GO_Central
  3. Golgi to vacuole transport Source: GO_Central
  4. intra-Golgi vesicle-mediated transport Source: GO_Central
  5. membrane fusion Source: ProtInc
  6. protein targeting to vacuole Source: GO_Central
  7. regulation of protein localization to plasma membrane Source: UniProtKB
  8. retrograde transport, endosome to Golgi Source: GO_Central
  9. vesicle docking involved in exocytosis Source: ProtInc
  10. vesicle fusion with Golgi apparatus Source: GO_Central
  11. vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1B
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 1
Vti1-rp1
Gene namesi
Name:VTI1B
Synonyms:VTI1, VTI1L, VTI1L1, VTI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:17793. VTI1B.

Subcellular locationi

Late endosome membrane 1 Publication; Single-pass type IV membrane protein 1 Publication. Lysosome membrane 1 Publication. Cytoplasmic granule 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 208207CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei209 – 22921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini230 – 2323VesicularSequence Analysis

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. endoplasmic reticulum membrane Source: GO_Central
  3. ER to Golgi transport vesicle membrane Source: GO_Central
  4. Golgi apparatus Source: UniProtKB
  5. integral component of membrane Source: UniProtKB-KW
  6. intracellular membrane-bounded organelle Source: HPA
  7. late endosome membrane Source: UniProtKB
  8. lysosomal membrane Source: UniProtKB
  9. neuronal cell body Source: ParkinsonsUK-UCL
  10. perinuclear region of cytoplasm Source: UniProtKB
  11. recycling endosome Source: UniProtKB
  12. SNARE complex Source: GO_Central
  13. synaptic vesicle Source: ParkinsonsUK-UCL
  14. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134861090.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 232231Vesicle transport through interaction with t-SNAREs homolog 1BPRO_0000218228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications
Modified residuei138 – 1381PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9UEU0.
PaxDbiQ9UEU0.
PRIDEiQ9UEU0.

PTM databases

PhosphoSiteiQ9UEU0.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiQ9UEU0.
ExpressionAtlasiQ9UEU0. baseline and differential.
GenevestigatoriQ9UEU0.

Organism-specific databases

HPAiHPA044121.
HPA048150.
HPA060118.

Interactioni

Subunit structurei

Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17.By similarity

Protein-protein interaction databases

BioGridi115753. 61 interactions.
IntActiQ9UEU0. 7 interactions.
MINTiMINT-1429590.
STRINGi9606.ENSP00000216456.

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni4 – 63Combined sources
Helixi9 – 2618Combined sources
Turni29 – 313Combined sources
Beta strandi32 – 343Combined sources
Helixi39 – 6628Combined sources
Helixi71 – 9323Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V8SX-ray2.22V1-96[»]
ProteinModelPortaliQ9UEU0.
SMRiQ9UEU0. Positions 2-94, 139-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UEU0.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili35 – 9864Sequence AnalysisAdd
BLAST
Coiled coili161 – 19838Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG291765.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG058837.
InParanoidiQ9UEU0.
KOiK08493.
OMAiYYKFFHK.
OrthoDBiEOG70KGQQ.
PhylomeDBiQ9UEU0.
TreeFamiTF312874.

Family and domain databases

InterProiIPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9UEU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSAASSEH FEKLHEIFRG LHEDLQGVPE RLLGTAGTEE KKKLIRDFDE
60 70 80 90 100
KQQEANETLA EMEEELRYAP LSFRNPMMSK LRNYRKDLAK LHREVRSTPL
110 120 130 140 150
TATPGGRGDM KYGIYAVENE HMNRLQSQRA MLLQGTESLN RATQSIERSH
160 170 180 190 200
RIATETDQIG SEIIEELGEQ RDQLERTKSR LVNTSENLSK SRKILRSMSR
210 220 230
KVTTNKLLLS IIILLELAIL GGLVYYKFFR SH
Length:232
Mass (Da):26,688
Last modified:February 20, 2007 - v3
Checksum:iB421C2863235B9B1
GO
Isoform Short (identifier: Q9UEU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:171
Mass (Da):19,810
Checksum:i7BC64529E0DBE12C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241D → N in AAC52016 (PubMed:9446565).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6161Missing in isoform Short. 1 PublicationVSP_006753Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA. Translation: AAC52016.1.
AF060902 mRNA. Translation: AAC73059.1.
CR456757 mRNA. Translation: CAG33038.1.
CR542095 mRNA. Translation: CAG46892.1.
BT019348 mRNA. Translation: AAV38155.1.
BC003142 mRNA. Translation: AAH03142.1.
CCDSiCCDS9786.1. [Q9UEU0-1]
RefSeqiNP_006361.1. NM_006370.2. [Q9UEU0-1]
UniGeneiHs.741177.

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568. [Q9UEU0-1]
GeneIDi10490.
KEGGihsa:10490.
UCSCiuc001xjt.3. human. [Q9UEU0-1]

Polymorphism databases

DMDMi126302613.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA. Translation: AAC52016.1.
AF060902 mRNA. Translation: AAC73059.1.
CR456757 mRNA. Translation: CAG33038.1.
CR542095 mRNA. Translation: CAG46892.1.
BT019348 mRNA. Translation: AAV38155.1.
BC003142 mRNA. Translation: AAH03142.1.
CCDSiCCDS9786.1. [Q9UEU0-1]
RefSeqiNP_006361.1. NM_006370.2. [Q9UEU0-1]
UniGeneiHs.741177.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V8SX-ray2.22V1-96[»]
ProteinModelPortaliQ9UEU0.
SMRiQ9UEU0. Positions 2-94, 139-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115753. 61 interactions.
IntActiQ9UEU0. 7 interactions.
MINTiMINT-1429590.
STRINGi9606.ENSP00000216456.

PTM databases

PhosphoSiteiQ9UEU0.

Polymorphism databases

DMDMi126302613.

Proteomic databases

MaxQBiQ9UEU0.
PaxDbiQ9UEU0.
PRIDEiQ9UEU0.

Protocols and materials databases

DNASUi10490.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568. [Q9UEU0-1]
GeneIDi10490.
KEGGihsa:10490.
UCSCiuc001xjt.3. human. [Q9UEU0-1]

Organism-specific databases

CTDi10490.
GeneCardsiGC14M068117.
HGNCiHGNC:17793. VTI1B.
HPAiHPA044121.
HPA048150.
HPA060118.
MIMi603207. gene.
neXtProtiNX_Q9UEU0.
PharmGKBiPA134861090.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG291765.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG058837.
InParanoidiQ9UEU0.
KOiK08493.
OMAiYYKFFHK.
OrthoDBiEOG70KGQQ.
PhylomeDBiQ9UEU0.
TreeFamiTF312874.

Miscellaneous databases

ChiTaRSiVTI1B. human.
EvolutionaryTraceiQ9UEU0.
GeneWikiiVTI1B.
GenomeRNAii10490.
NextBioi39804.
PROiQ9UEU0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UEU0.
ExpressionAtlasiQ9UEU0. baseline and differential.
GenevestigatoriQ9UEU0.

Family and domain databases

InterProiIPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways."
    Fischer von Mollard G., Stevens T.H.
    J. Biol. Chem. 273:2624-2630(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: Glioblastoma and Hypothalamus.
  2. "A hVti1 homologue: its expression depends on population doubling levels in both normal and SV40-transformed human fibroblasts."
    Li H.-C., Tahara H., Tsuyama N., Ide T.
    Biochem. Biophys. Res. Commun. 247:70-74(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Fibroblast.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Tissue: Kidney.
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes."
    Itakura E., Kishi-Itakura C., Mizushima N.
    Cell 151:1256-1269(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH STX17.
  11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiVTI1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UEU0
Secondary accession number(s): O43547, Q96J28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 20, 2007
Last modified: March 4, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.