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Protein

Vesicle transport through interaction with t-SNAREs homolog 1B

Gene

VTI1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

GO - Molecular functioni

  • chloride channel inhibitor activity Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI

GO - Biological processi

  • cell proliferation Source: ProtInc
  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • Golgi to vacuole transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • membrane fusion Source: ProtInc
  • protein targeting to vacuole Source: GO_Central
  • regulation of protein localization to plasma membrane Source: UniProtKB
  • retrograde transport, endosome to Golgi Source: GO_Central
  • vesicle docking involved in exocytosis Source: ProtInc
  • vesicle fusion with Golgi apparatus Source: GO_Central
  • vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100568-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1B
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 1
Vti1-rp1
Gene namesi
Name:VTI1B
Synonyms:VTI1, VTI1L, VTI1L1, VTI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:17793. VTI1B.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 208CytoplasmicSequence analysisAdd BLAST207
Transmembranei209 – 229Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini230 – 232VesicularSequence analysis3

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: GOC
  • endoplasmic reticulum membrane Source: GO_Central
  • ER to Golgi transport vesicle membrane Source: GO_Central
  • Golgi apparatus Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: HPA
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • neuronal cell body Source: ParkinsonsUK-UCL
  • perinuclear region of cytoplasm Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • SNARE complex Source: GO_Central
  • synaptic vesicle Source: ParkinsonsUK-UCL
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10490.
OpenTargetsiENSG00000100568.
PharmGKBiPA134861090.

Polymorphism and mutation databases

BioMutaiVTI1B.
DMDMi126302613.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002182282 – 232Vesicle transport through interaction with t-SNAREs homolog 1BAdd BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei103PhosphothreonineCombined sources1
Modified residuei107Omega-N-methylarginineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9UEU0.
PaxDbiQ9UEU0.
PeptideAtlasiQ9UEU0.
PRIDEiQ9UEU0.
TopDownProteomicsiQ9UEU0-1. [Q9UEU0-1]
Q9UEU0-2. [Q9UEU0-2]

PTM databases

iPTMnetiQ9UEU0.
PhosphoSitePlusiQ9UEU0.
SwissPalmiQ9UEU0.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiENSG00000100568.
ExpressionAtlasiQ9UEU0. baseline and differential.
GenevisibleiQ9UEU0. HS.

Organism-specific databases

HPAiHPA044121.
HPA048150.
HPA060118.

Interactioni

Subunit structurei

Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-7P604093EBI-723716,EBI-10172290
LDLRAD1Q5T7005EBI-723716,EBI-10173166
PTPN5P548293EBI-723716,EBI-1220572
STX4Q128465EBI-723716,EBI-744942
STX8Q9UNK06EBI-723716,EBI-727240
SYNE4Q8N2053EBI-723716,EBI-7131783
TRIM59Q8IWR15EBI-723716,EBI-10262539

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI

Protein-protein interaction databases

BioGridi115753. 86 interactors.
IntActiQ9UEU0. 25 interactors.
MINTiMINT-1429590.
STRINGi9606.ENSP00000450731.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Helixi9 – 26Combined sources18
Turni29 – 31Combined sources3
Beta strandi32 – 34Combined sources3
Helixi39 – 66Combined sources28
Helixi71 – 93Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V8SX-ray2.22V1-96[»]
ProteinModelPortaliQ9UEU0.
SMRiQ9UEU0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UEU0.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili35 – 98Sequence analysisAdd BLAST64
Coiled coili161 – 198Sequence analysisAdd BLAST38

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG058837.
InParanoidiQ9UEU0.
KOiK08493.
OMAiYYKFFHK.
OrthoDBiEOG091G12XX.
PhylomeDBiQ9UEU0.
TreeFamiTF312874.

Family and domain databases

InterProiIPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9UEU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSAASSEH FEKLHEIFRG LHEDLQGVPE RLLGTAGTEE KKKLIRDFDE
60 70 80 90 100
KQQEANETLA EMEEELRYAP LSFRNPMMSK LRNYRKDLAK LHREVRSTPL
110 120 130 140 150
TATPGGRGDM KYGIYAVENE HMNRLQSQRA MLLQGTESLN RATQSIERSH
160 170 180 190 200
RIATETDQIG SEIIEELGEQ RDQLERTKSR LVNTSENLSK SRKILRSMSR
210 220 230
KVTTNKLLLS IIILLELAIL GGLVYYKFFR SH
Length:232
Mass (Da):26,688
Last modified:February 20, 2007 - v3
Checksum:iB421C2863235B9B1
GO
Isoform Short (identifier: Q9UEU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:171
Mass (Da):19,810
Checksum:i7BC64529E0DBE12C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24D → N in AAC52016 (PubMed:9446565).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0067531 – 61Missing in isoform Short. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA. Translation: AAC52016.1.
AF060902 mRNA. Translation: AAC73059.1.
CR456757 mRNA. Translation: CAG33038.1.
CR542095 mRNA. Translation: CAG46892.1.
BT019348 mRNA. Translation: AAV38155.1.
BC003142 mRNA. Translation: AAH03142.1.
CCDSiCCDS9786.1. [Q9UEU0-1]
RefSeqiNP_006361.1. NM_006370.2. [Q9UEU0-1]
UniGeneiHs.741177.

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568. [Q9UEU0-1]
GeneIDi10490.
KEGGihsa:10490.
UCSCiuc001xjt.4. human. [Q9UEU0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA. Translation: AAC52016.1.
AF060902 mRNA. Translation: AAC73059.1.
CR456757 mRNA. Translation: CAG33038.1.
CR542095 mRNA. Translation: CAG46892.1.
BT019348 mRNA. Translation: AAV38155.1.
BC003142 mRNA. Translation: AAH03142.1.
CCDSiCCDS9786.1. [Q9UEU0-1]
RefSeqiNP_006361.1. NM_006370.2. [Q9UEU0-1]
UniGeneiHs.741177.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V8SX-ray2.22V1-96[»]
ProteinModelPortaliQ9UEU0.
SMRiQ9UEU0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115753. 86 interactors.
IntActiQ9UEU0. 25 interactors.
MINTiMINT-1429590.
STRINGi9606.ENSP00000450731.

PTM databases

iPTMnetiQ9UEU0.
PhosphoSitePlusiQ9UEU0.
SwissPalmiQ9UEU0.

Polymorphism and mutation databases

BioMutaiVTI1B.
DMDMi126302613.

Proteomic databases

EPDiQ9UEU0.
PaxDbiQ9UEU0.
PeptideAtlasiQ9UEU0.
PRIDEiQ9UEU0.
TopDownProteomicsiQ9UEU0-1. [Q9UEU0-1]
Q9UEU0-2. [Q9UEU0-2]

Protocols and materials databases

DNASUi10490.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568. [Q9UEU0-1]
GeneIDi10490.
KEGGihsa:10490.
UCSCiuc001xjt.4. human. [Q9UEU0-1]

Organism-specific databases

CTDi10490.
DisGeNETi10490.
GeneCardsiVTI1B.
HGNCiHGNC:17793. VTI1B.
HPAiHPA044121.
HPA048150.
HPA060118.
MIMi603207. gene.
neXtProtiNX_Q9UEU0.
OpenTargetsiENSG00000100568.
PharmGKBiPA134861090.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1666. Eukaryota.
ENOG4111J90. LUCA.
GeneTreeiENSGT00530000063466.
HOGENOMiHOG000116573.
HOVERGENiHBG058837.
InParanoidiQ9UEU0.
KOiK08493.
OMAiYYKFFHK.
OrthoDBiEOG091G12XX.
PhylomeDBiQ9UEU0.
TreeFamiTF312874.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100568-MONOMER.
ReactomeiR-HSA-114608. Platelet degranulation.

Miscellaneous databases

ChiTaRSiVTI1B. human.
EvolutionaryTraceiQ9UEU0.
GeneWikiiVTI1B.
GenomeRNAii10490.
PROiQ9UEU0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100568.
ExpressionAtlasiQ9UEU0. baseline and differential.
GenevisibleiQ9UEU0. HS.

Family and domain databases

InterProiIPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
IPR007705. Vesicle_trsprt_v-SNARE_N.
[Graphical view]
PfamiPF05008. V-SNARE. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVTI1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UEU0
Secondary accession number(s): O43547, Q96J28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.