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Protein

DnaJ homolog subfamily B member 4

Gene

DNAJB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable chaperone.

GO - Molecular functioni

  1. chaperone binding Source: UniProtKB
  2. unfolded protein binding Source: ProtInc

GO - Biological processi

  1. protein folding Source: InterPro
  2. response to heat Source: ProtInc
  3. response to unfolded protein Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 4
Alternative name(s):
Heat shock 40 kDa protein 1 homolog
Short name:
HSP40 homolog
Short name:
Heat shock protein 40 homolog
Human liver DnaJ-like protein
Gene namesi
Name:DNAJB4
Synonyms:DNAJW, HLJ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14886. DNAJB4.

Subcellular locationi

Cytoplasm. Cell membrane
Note: Cytoplasmic according to PubMed:18837411 and membrane-associated according to PubMed:16542645.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProtKB
  4. nucleoplasm Source: HPA
  5. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 337336DnaJ homolog subfamily B member 4PRO_0000071021Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei122 – 1221Phosphoserine2 Publications
Modified residuei148 – 1481Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UDY4.
PaxDbiQ9UDY4.
PRIDEiQ9UDY4.

PTM databases

PhosphoSiteiQ9UDY4.

Expressioni

Tissue specificityi

Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver.1 Publication

Inductioni

By heat shock.1 Publication

Gene expression databases

BgeeiQ9UDY4.
CleanExiHS_DNAJB4.
ExpressionAtlasiQ9UDY4. baseline and differential.
GenevestigatoriQ9UDY4.

Organism-specific databases

HPAiCAB004995.
HPA028383.
HPA028385.

Interactioni

Subunit structurei

Homodimer. The C-terminal section interacts with the C-terminal tail of OPRM1. Interacts also with SDIM1.2 Publications

Protein-protein interaction databases

BioGridi116263. 32 interactions.
IntActiQ9UDY4. 10 interactions.
MINTiMINT-1142940.
STRINGi9606.ENSP00000359799.

Structurei

3D structure databases

ProteinModelPortaliQ9UDY4.
SMRiQ9UDY4. Positions 1-74, 155-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7069JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2214.
GeneTreeiENSGT00760000118947.
HOVERGENiHBG066727.
InParanoidiQ9UDY4.
KOiK09510.
OMAiMAGGRDP.
PhylomeDBiQ9UDY4.
TreeFamiTF105141.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UDY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKDYYCILG IEKGASDEDI KKAYRKQALK FHPDKNKSPQ AEEKFKEVAE
60 70 80 90 100
AYEVLSDPKK REIYDQFGEE GLKGGAGGTD GQGGTFRYTF HGDPHATFAA
110 120 130 140 150
FFGGSNPFEI FFGRRMGGGR DSEEMEIDGD PFSAFGFSMN GYPRDRNSVG
160 170 180 190 200
PSRLKQDPPV IHELRVSLEE IYSGCTKRMK ISRKRLNADG RSYRSEDKIL
210 220 230 240 250
TIEIKKGWKE GTKITFPREG DETPNSIPAD IVFIIKDKDH PKFKRDGSNI
260 270 280 290 300
IYTAKISLRE ALCGCSINVP TLDGRNIPMS VNDIVKPGMR RRIIGYGLPF
310 320 330
PKNPDQRGDL LIEFEVSFPD TISSSSKEVL RKHLPAS
Length:337
Mass (Da):37,807
Last modified:April 30, 2000 - v1
Checksum:iC7A9C613F73BCDAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40992 mRNA. Translation: AAC14483.2.
AK313205 mRNA. Translation: BAG36021.1.
CH471059 Genomic DNA. Translation: EAX06354.1.
BC034721 mRNA. Translation: AAH34721.1.
U41290 Genomic DNA. Translation: AAB07346.1. Frameshift.
CCDSiCCDS684.1.
PIRiG02272.
RefSeqiNP_008965.2. NM_007034.3.
UniGeneiHs.13852.

Genome annotation databases

EnsembliENST00000370763; ENSP00000359799; ENSG00000162616.
GeneIDi11080.
KEGGihsa:11080.
UCSCiuc001dij.3. human.

Polymorphism databases

DMDMi8928155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40992 mRNA. Translation: AAC14483.2.
AK313205 mRNA. Translation: BAG36021.1.
CH471059 Genomic DNA. Translation: EAX06354.1.
BC034721 mRNA. Translation: AAH34721.1.
U41290 Genomic DNA. Translation: AAB07346.1. Frameshift.
CCDSiCCDS684.1.
PIRiG02272.
RefSeqiNP_008965.2. NM_007034.3.
UniGeneiHs.13852.

3D structure databases

ProteinModelPortaliQ9UDY4.
SMRiQ9UDY4. Positions 1-74, 155-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116263. 32 interactions.
IntActiQ9UDY4. 10 interactions.
MINTiMINT-1142940.
STRINGi9606.ENSP00000359799.

PTM databases

PhosphoSiteiQ9UDY4.

Polymorphism databases

DMDMi8928155.

Proteomic databases

MaxQBiQ9UDY4.
PaxDbiQ9UDY4.
PRIDEiQ9UDY4.

Protocols and materials databases

DNASUi11080.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370763; ENSP00000359799; ENSG00000162616.
GeneIDi11080.
KEGGihsa:11080.
UCSCiuc001dij.3. human.

Organism-specific databases

CTDi11080.
GeneCardsiGC01P078444.
HGNCiHGNC:14886. DNAJB4.
HPAiCAB004995.
HPA028383.
HPA028385.
MIMi611327. gene.
neXtProtiNX_Q9UDY4.
PharmGKBiPA27416.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2214.
GeneTreeiENSGT00760000118947.
HOVERGENiHBG066727.
InParanoidiQ9UDY4.
KOiK09510.
OMAiMAGGRDP.
PhylomeDBiQ9UDY4.
TreeFamiTF105141.

Miscellaneous databases

GeneWikiiDNAJB4.
GenomeRNAii11080.
NextBioi42126.
PROiQ9UDY4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UDY4.
CleanExiHS_DNAJB4.
ExpressionAtlasiQ9UDY4. baseline and differential.
GenevestigatoriQ9UDY4.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a new member of DnaJ-like heat shock protein 40 (Hsp40) from human liver."
    Hoe K.L., Won M., Chung K.S., Jang Y.J., Lee S.B., Kim D.U., Lee J.W., Yun J.H., Yoo H.S.
    Biochim. Biophys. Acta 1383:4-8(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph.
  5. Won M., Moon K.-M., Lee C.-E., Yoo H.-S.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  6. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (FEB-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-13; 45-59; 166-177; 219-236; 260-275 AND 293-302, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  7. "Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures."
    Borges J.C., Fischer H., Craievich A.F., Ramos C.H.I.
    J. Biol. Chem. 280:13671-13681(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION.
  8. "A member of the heat shock protein 40 family, hlj1, binds to the carboxyl tail of the human mu opioid receptor."
    Ancevska-Taneva N., Onoprishvili I., Andria M.L., Hiller J.M., Simon E.J.
    Brain Res. 1081:28-33(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH OPRM1.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Preparation of the anti-HLJ1 monoclonal antibodies and establishment of method for detection of the antigen."
    Lin X., Ma L., Wang J., Tan Y., Wen Q., Luo W., Su J., Lin Y., Wang X.
    Sheng Wu Gong Cheng Xue Bao 24:1293-1299(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122 AND SER-148, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "A novel neuron-enriched protein SDIM1 is down regulated in Alzheimer's brains and attenuates cell death induced by DNAJB4 over-expression in neuro-progenitor cells."
    Lei J.X., Cassone C.G., Luebbert C., Liu Q.Y.
    Mol. Neurodegener. 6:9-9(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SDIM1.

Entry informationi

Entry nameiDNJB4_HUMAN
AccessioniPrimary (citable) accession number: Q9UDY4
Secondary accession number(s): B2R824, Q13431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: April 30, 2000
Last modified: March 3, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.