Q9UDY4 (DNJB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily B member 4

Alternative name(s):
Heat shock 40 kDa protein 1 homolog
Short name=HSP40 homolog
Short name=Heat shock protein 40 homolog
Human liver DnaJ-like protein

Gene names

Name:	DNAJB4
Synonyms:	DNAJW, HLJ1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable chaperone.
Subunit structure	Homodimer. The C-terminal section interacts with the C-terminal tail of OPRM1. Interacts also with SDIM1. Ref.7 Ref.8 Ref.13
Subcellular location	Cytoplasm. Cell membrane. Note: Cytoplasmic according to Ref.10 and membrane-associated according to Ref.8. Ref.8 Ref.10
Tissue specificity	Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver. Ref.1
Induction	By heat shock. Ref.1
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell membrane Cytoplasm Membrane
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to heat Traceable author statement Ref.1. Source: ProtInc response to unfolded protein Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	unfolded protein binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 337	336	DnaJ homolog subfamily B member 4		PRO_0000071021
Regions
	Domain	2 – 70	69	J
Amino acid modifications
	Modified residue	122	1	Phosphoserine Ref.9 Ref.11
	Modified residue	148	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Q9UDY4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C7A9C613F73BCDAC	FASTA	337	37,807
	10 20 30 40 50 60 MGKDYYCILG IEKGASDEDI KKAYRKQALK FHPDKNKSPQ AEEKFKEVAE AYEVLSDPKK 70 80 90 100 110 120 REIYDQFGEE GLKGGAGGTD GQGGTFRYTF HGDPHATFAA FFGGSNPFEI FFGRRMGGGR 130 140 150 160 170 180 DSEEMEIDGD PFSAFGFSMN GYPRDRNSVG PSRLKQDPPV IHELRVSLEE IYSGCTKRMK 190 200 210 220 230 240 ISRKRLNADG RSYRSEDKIL TIEIKKGWKE GTKITFPREG DETPNSIPAD IVFIIKDKDH 250 260 270 280 290 300 PKFKRDGSNI IYTAKISLRE ALCGCSINVP TLDGRNIPMS VNDIVKPGMR RRIIGYGLPF 310 320 330 PKNPDQRGDL LIEFEVSFPD TISSSSKEVL RKHLPAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a new member of DnaJ-like heat shock protein 40 (Hsp40) from human liver." Hoe K.L., Won M., Chung K.S., Jang Y.J., Lee S.B., Kim D.U., Lee J.W., Yun J.H., Yoo H.S. Biochim. Biophys. Acta 1383:4-8(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[5]	Won M., Moon K.-M., Lee C.-E., Yoo H.-S. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[6]	Bienvenut W.V., Waridel P., Quadroni M. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 45-59; 166-177; 219-236; 260-275 AND 293-302, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures." Borges J.C., Fischer H., Craievich A.F., Ramos C.H.I. J. Biol. Chem. 280:13671-13681(2005) [PubMed] [Europe PMC] [Abstract] Cited for: HOMODIMERIZATION.
[8]	"A member of the heat shock protein 40 family, hlj1, binds to the carboxyl tail of the human mu opioid receptor." Ancevska-Taneva N., Onoprishvili I., Andria M.L., Hiller J.M., Simon E.J. Brain Res. 1081:28-33(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH OPRM1.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Preparation of the anti-HLJ1 monoclonal antibodies and establishment of method for detection of the antigen." Lin X., Ma L., Wang J., Tan Y., Wen Q., Luo W., Su J., Lin Y., Wang X. Sheng Wu Gong Cheng Xue Bao 24:1293-1299(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122 AND SER-148, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"A novel neuron-enriched protein SDIM1 is down regulated in Alzheimer's brains and attenuates cell death induced by DNAJB4 over-expression in neuro-progenitor cells." Lei J.X., Cassone C.G., Luebbert C., Liu Q.Y. Mol. Neurodegener. 6:9-9(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SDIM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40992 mRNA. Translation: AAC14483.2. AK313205 mRNA. Translation: BAG36021.1. CH471059 Genomic DNA. Translation: EAX06354.1. BC034721 mRNA. Translation: AAH34721.1. U41290 Genomic DNA. Translation: AAB07346.1. Frameshift.
IPI	IPI00003848.
PIR	G02272.
RefSeq	NP_008965.2. NM_007034.3.
UniGene	Hs.13852.
3D structure databases
ProteinModelPortal	Q9UDY4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UDY4. 8 interactions.
MINT	MINT-1142940.
STRING	9606.ENSP00000359799.
PTM databases
PhosphoSite	Q9UDY4.
Polymorphism databases
DMDM	8928155.
Proteomic databases
PaxDb	Q9UDY4.
PRIDE	Q9UDY4.
Protocols and materials databases
DNASU	11080.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370763; ENSP00000359799; ENSG00000162616.
GeneID	11080.
KEGG	hsa:11080.
UCSC	uc001dij.3. human.
Organism-specific databases
CTD	11080.
GeneCards	GC01P078444.
HGNC	HGNC:14886. DNAJB4.
HPA	CAB004995. HPA028383. HPA028385.
MIM	611327. gene.
neXtProt	NX_Q9UDY4.
PharmGKB	PA27416.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2214.
HOVERGEN	HBG066727.
InParanoid	Q9UDY4.
KO	K09510.
OMA	HFKAFEM.
OrthoDB	EOG4PVP00.
PhylomeDB	Q9UDY4.
Gene expression databases
ArrayExpress	Q9UDY4.
Bgee	Q9UDY4.
CleanEx	HS_DNAJB4.
Genevestigator	Q9UDY4.
GermOnline	ENSG00000162616. Homo sapiens.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR002939. DnaJ_C. IPR001623. DnaJ_domain. IPR018253. DnaJ_domain_CS. IPR008971. HSP40/DnaJ_pept-bd. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit. SSF49493. HSP40_DnaJ_pep. 2 hits.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11080.
NextBio	42126.
SOURCE	Search...

Entry information

Entry name

DNJB4_HUMAN

Accession

Primary (citable) accession number: Q9UDY4
Secondary accession number(s): B2R824, Q13431

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.