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Protein

CAP-Gly domain-containing linker protein 2

Gene

CLIP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to link microtubules to dendritic lamellar body (DLB), a membranous organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. May operate in the control of brain-specific organelle translocations (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
CAP-Gly domain-containing linker protein 2
Alternative name(s):
Cytoplasmic linker protein 115
Short name:
CLIP-115
Cytoplasmic linker protein 2
Williams-Beuren syndrome chromosomal region 3 protein
Williams-Beuren syndrome chromosomal region 4 protein
Gene namesi
Name:CLIP2
Synonyms:CYLN2, KIAA0291, WBSCR3, WBSCR4, WSCR4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:2586. CLIP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

CLIP2 is located in the Williams-Beuren syndrome (WBS) critical region (PubMed:9799601). WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of CLIP2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. However, it has been demonstrated that haploinsufficiency of this gene alone is not sufficient to cause any of the cognitive or facial features of WBS (PubMed:22608712).

Keywords - Diseasei

Williams-Beuren syndrome

Organism-specific databases

MalaCardsiCLIP2.
Orphaneti904. Williams syndrome.
PharmGKBiPA27085.

Polymorphism and mutation databases

BioMutaiCLIP2.
DMDMi74753268.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10461046CAP-Gly domain-containing linker protein 2PRO_0000083515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei202 – 2021PhosphoserineBy similarity
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei211 – 2111PhosphoserineBy similarity
Modified residuei314 – 3141PhosphoserineBy similarity
Modified residuei923 – 9231PhosphoserineBy similarity
Modified residuei973 – 9731PhosphoserineBy similarity
Modified residuei979 – 9791PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UDT6.
MaxQBiQ9UDT6.
PaxDbiQ9UDT6.
PRIDEiQ9UDT6.

PTM databases

iPTMnetiQ9UDT6.
PhosphoSiteiQ9UDT6.

Expressioni

Gene expression databases

BgeeiQ9UDT6.
CleanExiHS_CLIP2.
ExpressionAtlasiQ9UDT6. baseline and differential.
GenevisibleiQ9UDT6. HS.

Organism-specific databases

HPAiCAB020767.
HPA020430.

Interactioni

Subunit structurei

Interacts with CLASP1 and CLASP2.1 Publication

Protein-protein interaction databases

BioGridi113300. 10 interactions.
IntActiQ9UDT6. 7 interactions.
STRINGi9606.ENSP00000223398.

Structurei

Secondary structure

1
1046
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi73 – 753Combined sources
Beta strandi84 – 863Combined sources
Helixi87 – 893Combined sources
Beta strandi92 – 1009Combined sources
Beta strandi102 – 1043Combined sources
Beta strandi106 – 11611Combined sources
Beta strandi118 – 1247Combined sources
Turni134 – 1363Combined sources
Beta strandi137 – 1404Combined sources
Helixi142 – 1443Combined sources
Beta strandi224 – 2274Combined sources
Turni228 – 2303Combined sources
Beta strandi231 – 24010Combined sources
Beta strandi242 – 25615Combined sources
Beta strandi259 – 2646Combined sources
Beta strandi267 – 2704Combined sources
Turni274 – 2763Combined sources
Beta strandi277 – 2815Combined sources
Helixi282 – 2843Combined sources
Beta strandi285 – 2873Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP2NMR-A68-149[»]
2CP3NMR-A219-289[»]
ProteinModelPortaliQ9UDT6.
SMRiQ9UDT6. Positions 68-155, 173-344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UDT6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 14143CAP-Gly 1PROSITE-ProRule annotationAdd
BLAST
Domaini239 – 28143CAP-Gly 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili354 – 524171Sequence analysisAdd
BLAST
Coiled coili561 – 63676Sequence analysisAdd
BLAST
Coiled coili673 – 1016344Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi314 – 34532Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 CAP-Gly domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000092755.
HOVERGENiHBG007123.
InParanoidiQ9UDT6.
KOiK10422.
OMAiNMIESND.
PhylomeDBiQ9UDT6.
TreeFamiTF326096.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR028394. CLIP2.
[Graphical view]
PANTHERiPTHR18916:SF10. PTHR18916:SF10. 1 hit.
PfamiPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UDT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQKPSGLKPP GRGGKHSSPM GRTSTGSASS SAAVAASSKE GSPLHKQSSG
60 70 80 90 100
PSSSPAAAAA PEKPGPKAAE VGDDFLGDFV VGERVWVNGV KPGVVQYLGE
110 120 130 140 150
TQFAPGQWAG VVLDDPVGKN DGAVGGVRYF ECPALQGIFT RPSKLTRQPT
160 170 180 190 200
AEGSGSDAHS VESLTAQNLS LHSGTATPPL TSRVIPLRES VLNSSVKTGN
210 220 230 240 250
ESGSNLSDSG SVKRGEKDLR LGDRVLVGGT KTGVVRYVGE TDFAKGEWCG
260 270 280 290 300
VELDEPLGKN DGAVAGTRYF QCPPKFGLFA PIHKVIRIGF PSTSPAKAKK
310 320 330 340 350
TKRMAMGVSA LTHSPSSSSI SSVSSVASSV GGRPSRSGLL TETSSRYARK
360 370 380 390 400
ISGTTALQEA LKEKQQHIEQ LLAERDLERA EVAKATSHIC EVEKEIALLK
410 420 430 440 450
AQHEQYVAEA EEKLQRARLL VESVRKEKVD LSNQLEEERR KVEDLQFRVE
460 470 480 490 500
EESITKGDLE TQTQLEHARI GELEQSLLLE KAQAERLLRE LADNRLTTVA
510 520 530 540 550
EKSRVLQLEE ELTLRRGEIE ELQQCLLHSG PPPPDHPDAA EILRLRERLL
560 570 580 590 600
SASKEHQRES GVLRDKYEKA LKAYQAEVDK LRAANEKYAQ EVAGLKDKVQ
610 620 630 640 650
QATSENMGLM DNWKSKLDSL ASDHQKSLED LKATLNSGPG AQQKEIGELK
660 670 680 690 700
AVMEGIKMEH QLELGNLQAK HDLETAMHVK EKEALREKLQ EAQEELAGLQ
710 720 730 740 750
RHWRAQLEVQ ASQHRLELQE AQDQRRDAEL RVHELEKLDV EYRGQAQAIE
760 770 780 790 800
FLKEQISLAE KKMLDYERLQ RAEAQGKQEV ESLREKLLVA ENRLQAVEAL
810 820 830 840 850
CSSQHTHMIE SNDISEETIR TKETVEGLQD KLNKRDKEVT ALTSQTEMLR
860 870 880 890 900
AQVSALESKC KSGEKKVDAL LKEKRRLEAE LETVSRKTHD ASGQLVLISQ
910 920 930 940 950
ELLRKERSLN ELRVLLLEAN RHSPGPERDL SREVHKAEWR IKEQKLKDDI
960 970 980 990 1000
RGLREKLTGL DKEKSLSDQR RYSLIDRSSA PELLRLQHQL MSTEDALRDA
1010 1020 1030 1040
LDQAQQVEKL MEAMRSCPDK AQTIGNSGSA NGIHQQDKAQ KQEDKH
Length:1,046
Mass (Da):115,837
Last modified:May 1, 2000 - v1
Checksum:i7A0B3C796E1C6E25
GO
Isoform 2 (identifier: Q9UDT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     461-495: Missing.

Show »
Length:1,011
Mass (Da):111,782
Checksum:i2CFCD1B101162B1C
GO

Sequence cautioni

The sequence BAA22960.2 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti961 – 9611D → E.
Corresponds to variant rs17145468 [ dbSNP | Ensembl ].
VAR_055636
Natural varianti977 – 9771R → P.1 Publication
Corresponds to variant rs2522943 [ dbSNP | Ensembl ].
VAR_023618

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei461 – 49535Missing in isoform 2. 1 PublicationVSP_015682Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006629 mRNA. Translation: BAA22960.2. Different initiation.
AC005015 Genomic DNA. Translation: AAF03524.1.
AF041059
, AF041055, AF041056, AF041057, AF041058 Genomic DNA. Translation: AAB96784.1.
CCDSiCCDS5569.1. [Q9UDT6-1]
CCDS5570.1. [Q9UDT6-2]
RefSeqiNP_003379.3. NM_003388.4.
NP_115797.1. NM_032421.2.
UniGeneiHs.647018.

Genome annotation databases

EnsembliENST00000223398; ENSP00000223398; ENSG00000106665. [Q9UDT6-1]
ENST00000361545; ENSP00000355151; ENSG00000106665. [Q9UDT6-2]
ENST00000395060; ENSP00000378500; ENSG00000106665. [Q9UDT6-1]
GeneIDi7461.
KEGGihsa:7461.
UCSCiuc003uam.3. human. [Q9UDT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006629 mRNA. Translation: BAA22960.2. Different initiation.
AC005015 Genomic DNA. Translation: AAF03524.1.
AF041059
, AF041055, AF041056, AF041057, AF041058 Genomic DNA. Translation: AAB96784.1.
CCDSiCCDS5569.1. [Q9UDT6-1]
CCDS5570.1. [Q9UDT6-2]
RefSeqiNP_003379.3. NM_003388.4.
NP_115797.1. NM_032421.2.
UniGeneiHs.647018.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP2NMR-A68-149[»]
2CP3NMR-A219-289[»]
ProteinModelPortaliQ9UDT6.
SMRiQ9UDT6. Positions 68-155, 173-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113300. 10 interactions.
IntActiQ9UDT6. 7 interactions.
STRINGi9606.ENSP00000223398.

PTM databases

iPTMnetiQ9UDT6.
PhosphoSiteiQ9UDT6.

Polymorphism and mutation databases

BioMutaiCLIP2.
DMDMi74753268.

Proteomic databases

EPDiQ9UDT6.
MaxQBiQ9UDT6.
PaxDbiQ9UDT6.
PRIDEiQ9UDT6.

Protocols and materials databases

DNASUi7461.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223398; ENSP00000223398; ENSG00000106665. [Q9UDT6-1]
ENST00000361545; ENSP00000355151; ENSG00000106665. [Q9UDT6-2]
ENST00000395060; ENSP00000378500; ENSG00000106665. [Q9UDT6-1]
GeneIDi7461.
KEGGihsa:7461.
UCSCiuc003uam.3. human. [Q9UDT6-1]

Organism-specific databases

CTDi7461.
GeneCardsiCLIP2.
HGNCiHGNC:2586. CLIP2.
HPAiCAB020767.
HPA020430.
MalaCardsiCLIP2.
MIMi603432. gene.
neXtProtiNX_Q9UDT6.
Orphaneti904. Williams syndrome.
PharmGKBiPA27085.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000092755.
HOVERGENiHBG007123.
InParanoidiQ9UDT6.
KOiK10422.
OMAiNMIESND.
PhylomeDBiQ9UDT6.
TreeFamiTF326096.

Miscellaneous databases

ChiTaRSiCLIP2. human.
EvolutionaryTraceiQ9UDT6.
GeneWikiiCLIP2.
GenomeRNAii7461.
NextBioi29216.
PROiQ9UDT6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UDT6.
CleanExiHS_CLIP2.
ExpressionAtlasiQ9UDT6. baseline and differential.
GenevisibleiQ9UDT6. HS.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR028394. CLIP2.
[Graphical view]
PANTHERiPTHR18916:SF10. PTHR18916:SF10. 1 hit.
PfamiPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins."
    Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Nomura N.
    DNA Res. 4:53-59(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-977.
    Tissue: Brain.
  2. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Identification of genes from a 500-kb region at 7q11.23 that is commonly deleted in Williams syndrome patients."
    Osborne L.R., Martindale D.W., Scherer S.W., Shi X.-M., Huizenga J., Heng H.H.Q., Costa T., Pober B., Lew L., Brinkman J., Rommens J., Koop B.F., Tsui L.-C.
    Genomics 36:328-336(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-460.
  5. Lubec G., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 350-362 AND 689-701, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fetal brain cortex.
  6. "The murine CYLN2 gene: genomic organization, chromosome localization, and comparison to the human gene that is located within the 7q11.23 Williams syndrome critical region."
    Hoogenraad C.C., Eussen B.H.J., Langeveld A., van Haperen R., Winterberg S., Wouters C.H., Grosveld F., de Zeeuw C.I., Galjart N.
    Genomics 53:348-358(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE STRUCTURE.
  7. "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts."
    Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., de Zeeuw C.I., Grosveld F., Galjart N.
    Cell 104:923-935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CLASP1 AND CLASP2.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The contribution of CLIP2 haploinsufficiency to the clinical manifestations of the Williams-Beuren syndrome."
    Vandeweyer G., Van der Aa N., Reyniers E., Kooy R.F.
    Am. J. Hum. Genet. 90:1071-1078(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN WBS.
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. "Solution structure of the 1st and 2nd CAP-Gly domain in human CLIP-115/CYLN2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 68-149 AND 219-289.

Entry informationi

Entry nameiCLIP2_HUMAN
AccessioniPrimary (citable) accession number: Q9UDT6
Secondary accession number(s): O14527, O43611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: May 1, 2000
Last modified: April 13, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.