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Protein

Protein phosphatase 1 regulatory subunit 1B

Gene

PPP1R1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of protein-phosphatase 1.

GO - Molecular functioni

  • protein kinase inhibitor activity Source: UniProtKB
  • protein phosphatase inhibitor activity Source: UniProtKB
  • protein phosphatase type 1 regulator activity Source: Reactome
  • protein serine/threonine phosphatase inhibitor activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
SignaLinkiQ9UD71.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 1B
Alternative name(s):
DARPP-32
Dopamine- and cAMP-regulated neuronal phosphoprotein
Gene namesi
Name:PPP1R1B
Synonyms:DARPP32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:9287. PPP1R1B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33639.

Polymorphism and mutation databases

BioMutaiPPP1R1B.
DMDMi23831292.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Protein phosphatase 1 regulatory subunit 1BPRO_0000071473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei34 – 341Phosphothreonine; by PKABy similarity
Modified residuei75 – 751Phosphothreonine; by CDK51 Publication

Post-translational modificationi

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9UD71.
PaxDbiQ9UD71.
PRIDEiQ9UD71.

PTM databases

PhosphoSiteiQ9UD71.

Expressioni

Gene expression databases

BgeeiQ9UD71.
CleanExiHS_PPP1R1B.
ExpressionAtlasiQ9UD71. baseline and differential.
GenevisibleiQ9UD71. HS.

Organism-specific databases

HPAiCAB004026.
HPA048630.
HPA061142.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KLHL6Q8WZ603EBI-722119,EBI-6426464
TFCP2Q128003EBI-722119,EBI-717422

Protein-protein interaction databases

BioGridi123915. 6 interactions.
IntActiQ9UD71. 4 interactions.
MINTiMINT-1380912.
STRINGi9606.ENSP00000254079.

Structurei

3D structure databases

ProteinModelPortaliQ9UD71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 13618Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG39986.
GeneTreeiENSGT00730000111283.
HOGENOMiHOG000290193.
HOVERGENiHBG099182.
InParanoidiQ9UD71.
KOiK15494.
OMAiPAMLFRV.
OrthoDBiEOG7PCJJG.
PhylomeDBiQ9UD71.
TreeFamiTF332576.

Family and domain databases

InterProiIPR015670. Dopamine/cAMP_neuron_P-prot.
IPR008466. PPI_1DARPP-32.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PTHR15417:SF2. PTHR15417:SF2. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UD71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE
60 70 80 90 100
ASPHQRASGE GHHLKSKRPN PCAYTPPSLK AVQRIAESHL QSISNLNENQ
110 120 130 140 150
ASEEEDELGE LRELGYPREE DEEEEEDDEE EEEEEDSQAE VLKVIRQSAG
160 170 180 190 200
QKTTCGQGLE GPWERPPPLD ESERDGGSED QVEDPALSEP GEEPQRPSPS

EPGT
Length:204
Mass (Da):22,963
Last modified:October 10, 2002 - v2
Checksum:iE1CE7DDD29D2D4FB
GO
Isoform 2 (identifier: Q9UD71-2) [UniParc]FASTAAdd to basket

Also known as: t-DARPP

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Show »
Length:168
Mass (Da):18,738
Checksum:iF139B0673543BFE2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti155 – 1573CGQ → RGL (PubMed:8120638).Curated
Sequence conflicti204 – 2041T → R (PubMed:8120638).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3636Missing in isoform 2. 5 PublicationsVSP_005117Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464196 mRNA. Translation: AAM76683.1.
AY070271 mRNA. Translation: AAL59016.1.
AF435972 mRNA. Translation: AAP35094.1.
AF435973 mRNA. Translation: AAP35095.1.
AF435974 mRNA. Translation: AAP35096.1.
AF435975 Genomic DNA. Translation: AAP35097.1.
AF435975 Genomic DNA. Translation: AAP35098.1.
AK024593 mRNA. Translation: BAB14931.1.
BT007076 mRNA. Translation: AAP35739.1.
BC001519 mRNA. Translation: AAH01519.1.
CCDSiCCDS11339.1. [Q9UD71-1]
CCDS11340.1. [Q9UD71-2]
RefSeqiNP_001229393.1. NM_001242464.1. [Q9UD71-2]
NP_115568.2. NM_032192.3. [Q9UD71-1]
NP_852606.1. NM_181505.3. [Q9UD71-2]
XP_006722200.1. XM_006722137.2. [Q9UD71-1]
UniGeneiHs.286192.

Genome annotation databases

EnsembliENST00000254079; ENSP00000254079; ENSG00000131771.
ENST00000580825; ENSP00000462137; ENSG00000131771.
GeneIDi84152.
KEGGihsa:84152.
UCSCiuc002hrz.3. human. [Q9UD71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464196 mRNA. Translation: AAM76683.1.
AY070271 mRNA. Translation: AAL59016.1.
AF435972 mRNA. Translation: AAP35094.1.
AF435973 mRNA. Translation: AAP35095.1.
AF435974 mRNA. Translation: AAP35096.1.
AF435975 Genomic DNA. Translation: AAP35097.1.
AF435975 Genomic DNA. Translation: AAP35098.1.
AK024593 mRNA. Translation: BAB14931.1.
BT007076 mRNA. Translation: AAP35739.1.
BC001519 mRNA. Translation: AAH01519.1.
CCDSiCCDS11339.1. [Q9UD71-1]
CCDS11340.1. [Q9UD71-2]
RefSeqiNP_001229393.1. NM_001242464.1. [Q9UD71-2]
NP_115568.2. NM_032192.3. [Q9UD71-1]
NP_852606.1. NM_181505.3. [Q9UD71-2]
XP_006722200.1. XM_006722137.2. [Q9UD71-1]
UniGeneiHs.286192.

3D structure databases

ProteinModelPortaliQ9UD71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123915. 6 interactions.
IntActiQ9UD71. 4 interactions.
MINTiMINT-1380912.
STRINGi9606.ENSP00000254079.

PTM databases

PhosphoSiteiQ9UD71.

Polymorphism and mutation databases

BioMutaiPPP1R1B.
DMDMi23831292.

Proteomic databases

MaxQBiQ9UD71.
PaxDbiQ9UD71.
PRIDEiQ9UD71.

Protocols and materials databases

DNASUi84152.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254079; ENSP00000254079; ENSG00000131771.
ENST00000580825; ENSP00000462137; ENSG00000131771.
GeneIDi84152.
KEGGihsa:84152.
UCSCiuc002hrz.3. human. [Q9UD71-1]

Organism-specific databases

CTDi84152.
GeneCardsiGC17P037783.
HGNCiHGNC:9287. PPP1R1B.
HPAiCAB004026.
HPA048630.
HPA061142.
MIMi604399. gene.
neXtProtiNX_Q9UD71.
PharmGKBiPA33639.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39986.
GeneTreeiENSGT00730000111283.
HOGENOMiHOG000290193.
HOVERGENiHBG099182.
InParanoidiQ9UD71.
KOiK15494.
OMAiPAMLFRV.
OrthoDBiEOG7PCJJG.
PhylomeDBiQ9UD71.
TreeFamiTF332576.

Enzyme and pathway databases

ReactomeiREACT_15334. DARPP-32 events.
SignaLinkiQ9UD71.

Miscellaneous databases

ChiTaRSiPPP1R1B. human.
GeneWikiiPPP1R1B.
GenomeRNAii84152.
NextBioi73482.
PROiQ9UD71.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UD71.
CleanExiHS_PPP1R1B.
ExpressionAtlasiQ9UD71. baseline and differential.
GenevisibleiQ9UD71. HS.

Family and domain databases

InterProiIPR015670. Dopamine/cAMP_neuron_P-prot.
IPR008466. PPI_1DARPP-32.
[Graphical view]
PANTHERiPTHR15417. PTHR15417. 1 hit.
PTHR15417:SF2. PTHR15417:SF2. 1 hit.
PfamiPF05395. DARPP-32. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of mRNAs encoding ARPP-16/19, ARPP-21, and DARPP-32 in human brain tissue."
    Brene S., Lindefors N., Ehrlich M., Taubes T., Horiuchi A., Kopp J., Hall H., Sedvall G., Greengard P., Persson H.
    J. Neurosci. 14:985-998(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP."
    El-Rifai W., Smith M.F. Jr., Li G., Beckler A., Carl V.S., Montgomery E., Knuutila S., Moskaluk C.A., Frierson H.F. Jr., Powell S.M.
    Cancer Res. 62:4061-4064(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  3. "A family of genes encoding PKC-dependent protein phosphatase 1 (PP1) inhibitors."
    Liu Q.-R., Uhl G.R.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Adipose tissue.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon.
  7. Cited for: PHOSPHORYLATION AT THR-75 BY CDK5.

Entry informationi

Entry nameiPPR1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UD71
Secondary accession number(s): Q547V9, Q547W0, Q9H7G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 10, 2002
Last modified: July 22, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.