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Q9UD71 (PPR1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1 regulatory subunit 1B
Alternative name(s):
DARPP-32
Dopamine- and cAMP-regulated neuronal phosphoprotein
Gene names
Name:PPP1R1B
Synonyms:DARPP32
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of protein-phosphatase 1.

Subcellular location

Cytoplasm.

Post-translational modification

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.

Phosphorylation of Thr-34 is required for activity By similarity.

Sequence similarities

Belongs to the protein phosphatase inhibitor 1 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionProtein phosphatase inhibitor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from electronic annotation. Source: Ensembl

negative regulation of catalytic activity

Traceable author statement Ref.7. Source: GOC

negative regulation of female receptivity

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase activity

Traceable author statement Ref.7. Source: GOC

regulation of catalytic activity

Traceable author statement. Source: GOC

response to amphetamine

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement Ref.7. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

visual learning

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein kinase inhibitor activity

Traceable author statement Ref.7. Source: UniProtKB

protein phosphatase inhibitor activity

Traceable author statement Ref.7. Source: UniProtKB

protein phosphatase type 1 regulator activity

Traceable author statement. Source: Reactome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UD71-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UD71-2)

Also known as: t-DARPP;

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Protein phosphatase 1 regulatory subunit 1B
PRO_0000071473

Regions

Compositional bias119 – 13618Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue341Phosphothreonine; by PKA By similarity
Modified residue751Phosphothreonine; by CDK5 Ref.7

Natural variations

Alternative sequence1 – 3636Missing in isoform 2.
VSP_005117

Experimental info

Sequence conflict155 – 1573CGQ → RGL Ref.1
Sequence conflict2041T → R Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 10, 2002. Version 2.
Checksum: E1CE7DDD29D2D4FB

FASTA20422,963
        10         20         30         40         50         60 
MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE ASPHQRASGE 

        70         80         90        100        110        120 
GHHLKSKRPN PCAYTPPSLK AVQRIAESHL QSISNLNENQ ASEEEDELGE LRELGYPREE 

       130        140        150        160        170        180 
DEEEEEDDEE EEEEEDSQAE VLKVIRQSAG QKTTCGQGLE GPWERPPPLD ESERDGGSED 

       190        200 
QVEDPALSEP GEEPQRPSPS EPGT 

« Hide

Isoform 2 (t-DARPP) [UniParc].

Checksum: F139B0673543BFE2
Show »

FASTA16818,738

References

« Hide 'large scale' references
[1]"Expression of mRNAs encoding ARPP-16/19, ARPP-21, and DARPP-32 in human brain tissue."
Brene S., Lindefors N., Ehrlich M., Taubes T., Horiuchi A., Kopp J., Hall H., Sedvall G., Greengard P., Persson H.
J. Neurosci. 14:985-998(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP."
El-Rifai W., Smith M.F. Jr., Li G., Beckler A., Carl V.S., Montgomery E., Knuutila S., Moskaluk C.A., Frierson H.F. Jr., Powell S.M.
Cancer Res. 62:4061-4064(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]"A family of genes encoding PKC-dependent protein phosphatase 1 (PP1) inhibitors."
Liu Q.-R., Uhl G.R.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Adipose tissue.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Colon.
[7]"Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons."
Bibb J.A., Snyder G.L., Nishi A., Yan Z., Meijer L., Fienberg A.A., Tsai L.-H., Kwon Y.T., Girault J.-A., Czernik A.J., Huganir R.L., Hemmings H.C. Jr., Nairn A.C., Greengard P.
Nature 402:669-671(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-75 BY CDK5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF464196 mRNA. Translation: AAM76683.1.
AY070271 mRNA. Translation: AAL59016.1.
AF435972 mRNA. Translation: AAP35094.1.
AF435973 mRNA. Translation: AAP35095.1.
AF435974 mRNA. Translation: AAP35096.1.
AF435975 Genomic DNA. Translation: AAP35097.1.
AF435975 Genomic DNA. Translation: AAP35098.1.
AK024593 mRNA. Translation: BAB14931.1.
BT007076 mRNA. Translation: AAP35739.1.
BC001519 mRNA. Translation: AAH01519.1.
CCDSCCDS11339.1. [Q9UD71-1]
CCDS11340.1. [Q9UD71-2]
RefSeqNP_001229393.1. NM_001242464.1. [Q9UD71-2]
NP_115568.2. NM_032192.3. [Q9UD71-1]
NP_852606.1. NM_181505.3. [Q9UD71-2]
XP_006722200.1. XM_006722137.1. [Q9UD71-1]
UniGeneHs.286192.

3D structure databases

ProteinModelPortalQ9UD71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123915. 4 interactions.
IntActQ9UD71. 2 interactions.
MINTMINT-1380912.
STRING9606.ENSP00000254079.

PTM databases

PhosphoSiteQ9UD71.

Polymorphism databases

DMDM23831292.

Proteomic databases

MaxQBQ9UD71.
PaxDbQ9UD71.
PRIDEQ9UD71.

Protocols and materials databases

DNASU84152.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254079; ENSP00000254079; ENSG00000131771. [Q9UD71-1]
ENST00000394265; ENSP00000377808; ENSG00000131771. [Q9UD71-2]
ENST00000394267; ENSP00000377810; ENSG00000131771. [Q9UD71-2]
ENST00000580825; ENSP00000462137; ENSG00000131771. [Q9UD71-1]
GeneID84152.
KEGGhsa:84152.
UCSCuc002hrz.3. human. [Q9UD71-1]

Organism-specific databases

CTD84152.
GeneCardsGC17P037783.
HGNCHGNC:9287. PPP1R1B.
HPACAB004026.
HPA048630.
MIM604399. gene.
neXtProtNX_Q9UD71.
PharmGKBPA33639.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39986.
HOGENOMHOG000290193.
HOVERGENHBG099182.
KOK15494.
OMARAQLNSH.
OrthoDBEOG7PCJJG.
PhylomeDBQ9UD71.
TreeFamTF332576.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkQ9UD71.

Gene expression databases

ArrayExpressQ9UD71.
BgeeQ9UD71.
CleanExHS_PPP1R1B.
GenevestigatorQ9UD71.

Family and domain databases

InterProIPR015670. Dopamine/cAMP_neuron_P-prot.
IPR008466. PPI_1DARPP-32.
[Graphical view]
PANTHERPTHR15417. PTHR15417. 1 hit.
PTHR15417:SF2. PTHR15417:SF2. 1 hit.
PfamPF05395. DARPP-32. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPP1R1B. human.
GeneWikiPPP1R1B.
GenomeRNAi84152.
NextBio73482.
PROQ9UD71.
SOURCESearch...

Entry information

Entry namePPR1B_HUMAN
AccessionPrimary (citable) accession number: Q9UD71
Secondary accession number(s): Q547V9, Q547W0, Q9H7G1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 10, 2002
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM