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Q9UBY0 (SL9A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/hydrogen exchanger 2
Alternative name(s):
Na(+)/H(+) exchanger 2
Short name=NHE-2
Solute carrier family 9 member 2
Gene names
Name:SLC9A2
Synonyms:NHE2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length812 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.

Subunit structure

Interacts with CHP1 and CHP2 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in skeletal muscle, colon and kidney. Lower levels in the testis, prostate, ovary, and small intestine.

Post-translational modification

Phosphorylated (Possible).

Sequence similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. [View classification]

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 812812Sodium/hydrogen exchanger 2
PRO_0000052352

Regions

Topological domain1 – 1313Cytoplasmic Potential
Intramembrane14 – 3320Name=A/M1; By similarity
Topological domain34 – 7946Cytoplasmic Potential
Intramembrane80 – 10021Name=B/M2; By similarity
Topological domain101 – 1066Cytoplasmic Potential
Transmembrane107 – 12721Helical; Name=C/M3; Potential
Topological domain128 – 13811Extracellular Potential
Transmembrane139 – 15921Helical; Name=D/M4; Potential
Topological domain160 – 1689Cytoplasmic Potential
Transmembrane169 – 18921Helical; Name=E/M5; Potential
Topological domain190 – 20819Extracellular Potential
Transmembrane209 – 22921Helical; Name=F/M5A; Potential
Topological domain230 – 2367Cytoplasmic Potential
Transmembrane237 – 25721Helical; Name=G/M5B; Potential
Topological domain258 – 27720Extracellular Potential
Transmembrane278 – 29821Helical; Name=H/M6; Potential
Topological domain299 – 3079Cytoplasmic Potential
Transmembrane308 – 32821Helical; Name=I/M7; Potential
Topological domain329 – 36032Extracellular Potential
Transmembrane361 – 38121Helical; Name=J/M8; Potential
Topological domain382 – 39110Cytoplasmic Potential
Transmembrane392 – 41221Helical; Name=K/M9; Potential
Topological domain413 – 42917Extracellular Potential
Intramembrane430 – 45021Name=L; By similarity
Topological domain451 – 4588Extracellular Potential
Transmembrane459 – 47921Helical; Name=M13; Potential
Topological domain480 – 812333Cytoplasmic Potential

Amino acid modifications

Glycosylation3501N-linked (GlcNAc...) Potential

Natural variations

Natural variant2991T → S in a breast cancer sample; somatic mutation. Ref.5
VAR_035964
Natural variant8061R → Q in a breast cancer sample; somatic mutation. Ref.5
VAR_035965

Sequences

Sequence LengthMass (Da)Tools
Q9UBY0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 17EE177DC3830D0A

FASTA81291,520
        10         20         30         40         50         60 
MEPLGNWRSL RAPLPPMLLL LLLQVAGPVG ALAETLLNAP RAMGTSSSPP SPASVVAPGT 

        70         80         90        100        110        120 
TLFEESRLPV FTLDYPHVQI PFEITLWILL ASLAKIGFHL YHKLPTIVPE SCLLIMVGLL 

       130        140        150        160        170        180 
LGGIIFGVDE KSPPAMKTDV FFLYLLPPIV LDAGYFMPTR PFFENIGTIF WYAVVGTLWN 

       190        200        210        220        230        240 
SIGIGVSLFG ICQIEAFGLS DITLLQNLLF GSLISAVDPV AVLAVFENIH VNEQLYILVF 

       250        260        270        280        290        300 
GESLLNDAVT VVLYNLFKSF CQMKTIETID VFAGIANFFV VGIGGVLIGI FLGFIAAFTT 

       310        320        330        340        350        360 
RFTHNIRVIE PLFVFLYSYL SYITAEMFHL SGIMAITACA MTMNKYVEEN VSQKSYTTIK 

       370        380        390        400        410        420 
YFMKMLSSVS ETLIFIFMGV STVGKNHEWN WAFVCFTLAF CLMWRALGVF VLTQVINRFR 

       430        440        450        460        470        480 
TIPLTFKDQF IIAYGGLRGA ICFALVFLLP AAVFPRKKLF ITAAIVVIFF TVFILGITIR 

       490        500        510        520        530        540 
PLVEFLDVKR SNKKQQAVSE EIYCRLFDHV KTGIEDVCGH WGHNFWRDKF KKFDDKYLRK 

       550        560        570        580        590        600 
LLIRENQPKS SIVSLYKKLE IKHAIEMAET GMISTVPTFA SLNDCREEKI RKVTSSETDE 

       610        620        630        640        650        660 
IRELLSRNLY QIRQRTLSYN RHSLTADTSE RQAKEILIRR RHSLRESIRK DSSLNREHRA 

       670        680        690        700        710        720 
STSTSRYLSL PKNTKLPEKL QKRRTISIAD GNSSDSDADA GTTVLNLQPR ARRFLPEQFS 

       730        740        750        760        770        780 
KKSPQSYKME WKNEVDVDSG RDMPSTPPTP HSREKGTQTS GLLQQPLLSK DQSGSEREDS 

       790        800        810 
LTEGIPPKPP PRLVWRASEP GSRKARFGSE KP 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, tissue distribution, and functional expression of the human Na(+)/H(+) exchanger NHE2."
Malakooti J., Dahdal R.Y., Schmidt L., Layden T.J., Dudeja P.K., Ramaswamy K.
Am. J. Physiol. 277:G383-G390(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Colon.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-299 AND GLN-806.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF073299 mRNA. Translation: AAD41635.1.
AC007239 Genomic DNA. Translation: AAF19248.1.
CH471127 Genomic DNA. Translation: EAX01789.1.
BC136377 mRNA. Translation: AAI36378.1.
BC136378 mRNA. Translation: AAI36379.1.
RefSeqNP_003039.2. NM_003048.3.
UniGeneHs.250083.

3D structure databases

ProteinModelPortalQ9UBY0.
SMRQ9UBY0. Positions 135-160, 230-255, 318-345, 483-524, 591-653.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112439. 7 interactions.
IntActQ9UBY0. 1 interaction.
STRING9606.ENSP00000233969.

Chemistry

BindingDBQ9UBY0.
ChEMBLCHEMBL3133.

Protein family/group databases

TCDB2.A.36.1.17. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSiteQ9UBY0.

Polymorphism databases

DMDM19924293.

Proteomic databases

PaxDbQ9UBY0.
PRIDEQ9UBY0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233969; ENSP00000233969; ENSG00000115616.
GeneID6549.
KEGGhsa:6549.
UCSCuc002tca.3. human.

Organism-specific databases

CTD6549.
GeneCardsGC02P103236.
HGNCHGNC:11072. SLC9A2.
HPAHPA035121.
MIM600530. gene.
neXtProtNX_Q9UBY0.
PharmGKBPA35929.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0025.
HOGENOMHOG000247044.
HOVERGENHBG052615.
InParanoidQ9UBY0.
KOK14722.
OMAPQSYKME.
OrthoDBEOG7PCJGB.
PhylomeDBQ9UBY0.
TreeFamTF317212.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

BgeeQ9UBY0.
CleanExHS_SLC9A2.
GenevestigatorQ9UBY0.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001953. Na/H_exchanger_2.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERPTHR10110. PTHR10110. 1 hit.
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSPR01084. NAHEXCHNGR.
PR01086. NAHEXCHNGR2.
TIGRFAMsTIGR00840. b_cpa1. 1 hit.
ProtoNetSearch...

Other

GeneWikiSLC9A2.
GenomeRNAi6549.
NextBio25489.
PROQ9UBY0.
SOURCESearch...

Entry information

Entry nameSL9A2_HUMAN
AccessionPrimary (citable) accession number: Q9UBY0
Secondary accession number(s): B2RMS2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM