Q9UBX7 (KLK11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-11
Short name=hK11
EC=3.4.21.-

Alternative name(s):
Hippostasin
Serine protease 20
Trypsin-like protease

Cleaved into the following 2 chains:

1. Kallikrein-11 inactive chain 1
2. Kallikrein-11 inactive chain 2

Gene names

Name:	KLK11
Synonyms:	PRSS20, TLSP
ORF Names:	UNQ649/PRO1279

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Ref.2 Ref.11
Subcellular location	Isoform 1: Secreted Ref.6. Isoform 2: Golgi apparatus Ref.6.
Tissue specificity	Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Ref.2 Ref.11
Post-translational modification	About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q9UBX7-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9UBX7-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Isoform 3 (identifier: Q9UBX7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: P → PWVSLTSPTHVSPDLSSSNYCLSHLS

Note: Localized in the prostate secretory epithelium.

Isoform 4 (identifier: Q9UBX7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     98-98: P → PWVSLTSPTHVSPDLSSSNYCLSHLS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 50	50	Potential
	Propeptide	51 – 53	3	Activation peptide Potential		PRO_0000027954
	Chain	54 – 282	229	Kallikrein-11		PRO_0000027955
	Chain	54 – 188	135	Kallikrein-11 inactive chain 1		PRO_0000302061
	Chain	189 – 282	94	Kallikrein-11 inactive chain 2		PRO_0000302062
Regions
	Domain	53 – 280	228	Peptidase S1
Sites
	Active site	94	1	Charge relay system By similarity
	Active site	142	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 195		By similarity
	Disulfide bond	79 ↔ 95		By similarity
	Disulfide bond	167 ↔ 269		By similarity
	Disulfide bond	174 ↔ 241		By similarity
	Disulfide bond	206 ↔ 220		By similarity
	Disulfide bond	231 ↔ 256		By similarity
Natural variations
	Alternative sequence	1 – 32	32	Missing in isoform 1 and isoform 4.		VSP_005402
	Alternative sequence	98	1	P → PWVSLTSPTHVSPDLSSSNY CLSHLS in isoform 3 and isoform 4.		VSP_043326
	Natural variant	32	1	A → T. Corresponds to variant rs2288892 [ dbSNP | Ensembl ].		VAR_051856
	Natural variant	49	1	G → E. Corresponds to variant rs3745539 [ dbSNP | Ensembl ].		VAR_021943
	Natural variant	166	1	R → C. Corresponds to variant rs1048328 [ dbSNP | Ensembl ].		VAR_024296

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: B442DDE613EE0A17	FASTA	282	31,059
	10 20 30 40 50 60 MQRLRWLRDW KSSGRGLTAA KEPGARSSPL QAMRILQLIL LALATGLVGG ETRIIKGFEC 70 80 90 100 110 120 KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT 130 140 150 160 170 180 ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG 190 200 210 220 230 240 STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV 250 260 270 280 CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN
	Isoform 1 [UniParc]. Checksum: 192D910BBCDC7A56	FASTA	250	27,466
	10 20 30 40 50 60 MRILQLILLA LATGLVGGET RIIKGFECKP HSQPWQAALF EKTRLLCGAT LIAPRWLLTA 70 80 90 100 110 120 AHCLKPRYIV HLGQHNLQKE EGCEQTRTAT ESFPHPGFNN SLPNKDHRND IMLVKMASPV 130 140 150 160 170 180 SITWAVRPLT LSSRCVTAGT SCLISGWGST SSPQLRLPHT LRCANITIIE HQKCENAYPG 190 200 210 220 230 240 NITDTMVCAS VQEGGKDSCQ GDSGGPLVCN QSLQGIISWG QDPCAITRKP GVYTKVCKYV 250 DWIQETMKNN
	Isoform 3 [UniParc]. Checksum: E5C0EE949DBAFA2B	FASTA	307	33,759
	10 20 30 40 50 60 MQRLRWLRDW KSSGRGLTAA KEPGARSSPL QAMRILQLIL LALATGLVGG ETRIIKGFEC 70 80 90 100 110 120 KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPWV SLTSPTHVSP DLSSSNYCLS 130 140 150 160 170 180 HLSRYIVHLG QHNLQKEEGC EQTRTATESF PHPGFNNSLP NKDHRNDIML VKMASPVSIT 190 200 210 220 230 240 WAVRPLTLSS RCVTAGTSCL ISGWGSTSSP QLRLPHTLRC ANITIIEHQK CENAYPGNIT 250 260 270 280 290 300 DTMVCASVQE GGKDSCQGDS GGPLVCNQSL QGIISWGQDP CAITRKPGVY TKVCKYVDWI QETMKNN
	Isoform 4 [UniParc]. Checksum: 257A42B28F40E2C4	FASTA	275	30,166
	10 20 30 40 50 60 MRILQLILLA LATGLVGGET RIIKGFECKP HSQPWQAALF EKTRLLCGAT LIAPRWLLTA 70 80 90 100 110 120 AHCLKPWVSL TSPTHVSPDL SSSNYCLSHL SRYIVHLGQH NLQKEEGCEQ TRTATESFPH 130 140 150 160 170 180 PGFNNSLPNK DHRNDIMLVK MASPVSITWA VRPLTLSSRC VTAGTSCLIS GWGSTSSPQL 190 200 210 220 230 240 RLPHTLRCAN ITIIEHQKCE NAYPGNITDT MVCASVQEGG KDSCQGDSGG PLVCNQSLQG 250 260 270 IISWGQDPCA ITRKPGVYTK VCKYVDWIQE TMKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of a novel serine protease, TLSP." Yoshida S., Taniguchi M., Suemoto T., Oka T., He X.P., Shiosaka S. Biochim. Biophys. Acta 1399:225-228(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Hippocampus.
[2]	"A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate." Mitsui S., Yamada T., Okui A., Kominami K., Uemura H., Yamaguchi N. Biochem. Biophys. Res. Commun. 272:205-211(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Hippocampus and Prostate.
[3]	"Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family." Yousef G.M., Scorilas A., Diamandis E.P. Genomics 63:88-96(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Molecular cloning and expression of a variant form of hippostasin/KLK11 in prostate." Nakamura T., Mitsui S., Okui A., Miki T., Yamaguchi N. Prostate 54:299-305(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[6]	"Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin." Mitsui S., Nakamura T., Okui A., Kominami K., Uemura H., Yamaguchi N. FEBS J. 273:3678-3686(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
[7]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[11]	"Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma." Luo L.Y., Shan S.J., Elliott M.B., Soosaipillai A., Diamandis E.P. Clin. Cancer Res. 12:742-750(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PROTEOLYTIC PROCESSING, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB012917 mRNA. Translation: BAA33404.1. AB013730 mRNA. Translation: BAA88713.1. AB041036 mRNA. Translation: BAA96797.1. AF164623 Genomic DNA. Translation: AAD47815.1. AF243527 Genomic DNA. Translation: AAG33364.1. AB078780 mRNA. Translation: BAC54105.1. AB261897 mRNA. Translation: BAE95335.1. AB259014 mRNA. Translation: BAF02733.1. AY359014 mRNA. Translation: AAQ89373.1. AC011473 Genomic DNA. Translation: AAG23257.1. CH471135 Genomic DNA. Translation: EAW71971.1. BC022068 mRNA. Translation: AAH22068.1.
IPI	IPI00002818. IPI00217503. IPI00218137.
RefSeq	NP_001129504.1. NM_001136032.2. NP_001161077.1. NM_001167605.1. NP_006844.1. NM_006853.2. NP_659196.1. NM_144947.1.
UniGene	Hs.57771.
3D structure databases
ProteinModelPortal	Q9UBX7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000324414.
Protein family/group databases
MEROPS	S01.257.
PTM databases
PhosphoSite	Q9UBX7.
Polymorphism databases
DMDM	88984315.
Proteomic databases
PaxDb	Q9UBX7.
PRIDE	Q9UBX7.
Protocols and materials databases
DNASU	11012.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319720; ENSP00000324269; ENSG00000167757. ENST00000391804; ENSP00000375680; ENSG00000167757. ENST00000453757; ENSP00000413958; ENSG00000167757. ENST00000594768; ENSP00000473047; ENSG00000167757.
GeneID	11012.
KEGG	hsa:11012.
UCSC	uc002pvc.4. human.
Organism-specific databases
CTD	11012.
GeneCards	GC19M051525.
HGNC	HGNC:6359. KLK11.
HPA	CAB026340.
MIM	604434. gene.
neXtProt	NX_Q9UBX7.
PharmGKB	PA30148.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q9UBX7.
KO	K09620.
OMA	MVCASVK.
OrthoDB	EOG4XKV7F.
PhylomeDB	Q9UBX7.
Gene expression databases
ArrayExpress	Q9UBX7.
Bgee	Q9UBX7.
CleanEx	HS_KLK11.
Genevestigator	Q9UBX7.
GermOnline	ENSG00000167757. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9UBX7.
ChEMBL	CHEMBL3031.
GenomeRNAi	11012.
NextBio	41831.
PMAP-CutDB	Q9UBX7.
SOURCE	Search...

Entry information

Entry name

KLK11_HUMAN

Accession

Primary (citable) accession number: Q9UBX7
Secondary accession number(s): O75837 , Q0WXX5, Q8IXD7, Q9NS65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 21, 2006
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.