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Q9UBX7 (KLK11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-11

Short name=hK11
EC=3.4.21.-
Alternative name(s):
Hippostasin
Serine protease 20
Trypsin-like protease
Gene names
Name:KLK11
Synonyms:PRSS20, TLSP
ORF Names:UNQ649/PRO1279
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Ref.2 Ref.11

Subcellular location

Isoform 1: Secreted Ref.6.

Isoform 2: Golgi apparatus Ref.6.

Tissue specificity

Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Ref.2 Ref.11

Post-translational modification

About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

serine-type peptidase activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q9UBX7-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q9UBX7-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
Isoform 3 (identifier: Q9UBX7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: P → PWVSLTSPTHVSPDLSSSNYCLSHLS
Note: Localized in the prostate secretory epithelium.
Isoform 4 (identifier: Q9UBX7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     98-98: P → PWVSLTSPTHVSPDLSSSNYCLSHLS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5050 Potential
Propeptide51 – 533Activation peptide Potential
PRO_0000027954
Chain54 – 282229Kallikrein-11
PRO_0000027955
Chain54 – 188135Kallikrein-11 inactive chain 1
PRO_0000302061
Chain189 – 28294Kallikrein-11 inactive chain 2
PRO_0000302062

Regions

Domain53 – 280228Peptidase S1

Sites

Active site941Charge relay system By similarity
Active site1421Charge relay system By similarity
Active site2351Charge relay system By similarity

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2421N-linked (GlcNAc...) Potential
Disulfide bond60 ↔ 195 By similarity
Disulfide bond79 ↔ 95 By similarity
Disulfide bond167 ↔ 269 By similarity
Disulfide bond174 ↔ 241 By similarity
Disulfide bond206 ↔ 220 By similarity
Disulfide bond231 ↔ 256 By similarity

Natural variations

Alternative sequence1 – 3232Missing in isoform 1 and isoform 4.
VSP_005402
Alternative sequence981P → PWVSLTSPTHVSPDLSSSNY CLSHLS in isoform 3 and isoform 4.
VSP_043326
Natural variant321A → T.
Corresponds to variant rs2288892 [ dbSNP | Ensembl ].
VAR_051856
Natural variant491G → E.
Corresponds to variant rs3745539 [ dbSNP | Ensembl ].
VAR_021943
Natural variant1661R → C.
Corresponds to variant rs1048328 [ dbSNP | Ensembl ].
VAR_024296

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified February 21, 2006. Version 2.
Checksum: B442DDE613EE0A17

FASTA28231,059
        10         20         30         40         50         60 
MQRLRWLRDW KSSGRGLTAA KEPGARSSPL QAMRILQLIL LALATGLVGG ETRIIKGFEC 

        70         80         90        100        110        120 
KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT 

       130        140        150        160        170        180 
ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG 

       190        200        210        220        230        240 
STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV 

       250        260        270        280 
CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN 

« Hide

Isoform 1 [UniParc].

Checksum: 192D910BBCDC7A56
Show »

FASTA25027,466
Isoform 3 [UniParc].

Checksum: E5C0EE949DBAFA2B
Show »

FASTA30733,759
Isoform 4 [UniParc].

Checksum: 257A42B28F40E2C4
Show »

FASTA27530,166

References

« Hide 'large scale' references
[1]"cDNA cloning and expression of a novel serine protease, TLSP."
Yoshida S., Taniguchi M., Suemoto T., Oka T., He X.P., Shiosaka S.
Biochim. Biophys. Acta 1399:225-228(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Hippocampus.
[2]"A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate."
Mitsui S., Yamada T., Okui A., Kominami K., Uemura H., Yamaguchi N.
Biochem. Biophys. Res. Commun. 272:205-211(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Hippocampus and Prostate.
[3]"Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family."
Yousef G.M., Scorilas A., Diamandis E.P.
Genomics 63:88-96(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region."
Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K.
Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Molecular cloning and expression of a variant form of hippostasin/KLK11 in prostate."
Nakamura T., Mitsui S., Okui A., Miki T., Yamaguchi N.
Prostate 54:299-305(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[6]"Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin."
Mitsui S., Nakamura T., Okui A., Kominami K., Uemura H., Yamaguchi N.
FEBS J. 273:3678-3686(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
[7]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[11]"Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma."
Luo L.Y., Shan S.J., Elliott M.B., Soosaipillai A., Diamandis E.P.
Clin. Cancer Res. 12:742-750(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PROTEOLYTIC PROCESSING, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012917 mRNA. Translation: BAA33404.1.
AB013730 mRNA. Translation: BAA88713.1.
AB041036 mRNA. Translation: BAA96797.1.
AF164623 Genomic DNA. Translation: AAD47815.1.
AF243527 Genomic DNA. Translation: AAG33364.1.
AB078780 mRNA. Translation: BAC54105.1.
AB261897 mRNA. Translation: BAE95335.1.
AB259014 mRNA. Translation: BAF02733.1.
AY359014 mRNA. Translation: AAQ89373.1.
AC011473 Genomic DNA. Translation: AAG23257.1.
CH471135 Genomic DNA. Translation: EAW71971.1.
BC022068 mRNA. Translation: AAH22068.1.
CCDSCCDS12818.1. [Q9UBX7-2]
CCDS12819.1. [Q9UBX7-1]
CCDS54297.1. [Q9UBX7-4]
RefSeqNP_001129504.1. NM_001136032.2. [Q9UBX7-1]
NP_001161077.1. NM_001167605.1. [Q9UBX7-4]
NP_006844.1. NM_006853.2. [Q9UBX7-1]
NP_659196.1. NM_144947.1. [Q9UBX7-2]
UniGeneHs.57771.

3D structure databases

ProteinModelPortalQ9UBX7.
SMRQ9UBX7. Positions 17-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000324414.

Chemistry

BindingDBQ9UBX7.

Protein family/group databases

MEROPSS01.257.

PTM databases

PhosphoSiteQ9UBX7.

Polymorphism databases

DMDM88984315.

Proteomic databases

PaxDbQ9UBX7.
PRIDEQ9UBX7.

Protocols and materials databases

DNASU11012.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000319720; ENSP00000324269; ENSG00000167757. [Q9UBX7-1]
ENST00000391804; ENSP00000375680; ENSG00000167757. [Q9UBX7-4]
ENST00000453757; ENSP00000413958; ENSG00000167757. [Q9UBX7-1]
ENST00000594768; ENSP00000473047; ENSG00000167757. [Q9UBX7-2]
GeneID11012.
KEGGhsa:11012.
UCSCuc002pvb.2. human. [Q9UBX7-3]
uc002pvc.4. human. [Q9UBX7-2]

Organism-specific databases

CTD11012.
GeneCardsGC19M051525.
HGNCHGNC:6359. KLK11.
HPACAB026340.
MIM604434. gene.
neXtProtNX_Q9UBX7.
PharmGKBPA30148.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ9UBX7.
KOK09620.
OMASSRCVTA.
PhylomeDBQ9UBX7.
TreeFamTF331065.

Gene expression databases

ArrayExpressQ9UBX7.
BgeeQ9UBX7.
CleanExHS_KLK11.
GenevestigatorQ9UBX7.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLK11.
GenomeRNAi11012.
NextBio41831.
PMAP-CutDBQ9UBX7.
PROQ9UBX7.
SOURCESearch...

Entry information

Entry nameKLK11_HUMAN
AccessionPrimary (citable) accession number: Q9UBX7
Secondary accession number(s): O75837 expand/collapse secondary AC list , Q0WXX5, Q8IXD7, Q9NS65
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 21, 2006
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM