Reviewed, UniProtKB/Swiss-Prot Q9UBX7 (KLK11_HUMAN)

Names and origin

Protein names

Recommended name:
    Kallikrein-11
      Short name=hK11
    EC=3.4.21.-
Alternative name(s):
    Hippostasin
    Trypsin-like protease
    Serine protease 20
Cleaved into the following 2 chains:
    1- Recommended name:
            Kallikrein-11 inactive chain 1
    2- Recommended name:
            Kallikrein-11 inactive chain 2

Gene names

Name:	KLK11
Synonyms:	PRSS20, TLSP
ORF Names:	UNQ649/PRO1279

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Ref.2 Ref.8
Subcellular location	Secreted.
Tissue specificity	Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain; isoform 2 in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). Ref.2 Ref.8
Post-translational modification	About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q9UBX7-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9UBX7-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 50	50	Potential
	Propeptide	51 – 53	3	Activation peptide Potential		PRO_0000027954
	Chain	54 – 282	229	Kallikrein-11		PRO_0000027955
	Chain	54 – 188	135	Kallikrein-11 inactive chain 1		PRO_0000302061
	Chain	189 – 282	94	Kallikrein-11 inactive chain 2		PRO_0000302062
Regions
	Domain	53 – 280	228	Peptidase S1
Sites
	Active site	94	1	Charge relay system By similarity
	Active site	142	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 195		By similarity
	Disulfide bond	79 ↔ 95		By similarity
	Disulfide bond	167 ↔ 269		By similarity
	Disulfide bond	174 ↔ 241		By similarity
	Disulfide bond	206 ↔ 220		By similarity
	Disulfide bond	231 ↔ 256		By similarity
Natural variations
	Alternative sequence	1 – 32	32	Missing in isoform 1.		VSP_005402
	Natural variant	32	1	A → T: dbSNP rs2288892.		VAR_051856
	Natural variant	49	1	G → E: dbSNP rs3745539.		VAR_021943
	Natural variant	166	1	R → C: dbSNP rs1048328.		VAR_024296

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: B442DDE613EE0A17	FASTA	282	31,059
	10 20 30 40 50 60 MQRLRWLRDW KSSGRGLTAA KEPGARSSPL QAMRILQLIL LALATGLVGG ETRIIKGFEC 70 80 90 100 110 120 KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT 130 140 150 160 170 180 ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG 190 200 210 220 230 240 STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV 250 260 270 280 CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN
	Isoform 1. Checksum: 192D910BBCDC7A56	FASTA	250	27,466
	10 20 30 40 50 60 MRILQLILLA LATGLVGGET RIIKGFECKP HSQPWQAALF EKTRLLCGAT LIAPRWLLTA 70 80 90 100 110 120 AHCLKPRYIV HLGQHNLQKE EGCEQTRTAT ESFPHPGFNN SLPNKDHRND IMLVKMASPV 130 140 150 160 170 180 SITWAVRPLT LSSRCVTAGT SCLISGWGST SSPQLRLPHT LRCANITIIE HQKCENAYPG 190 200 210 220 230 240 NITDTMVCAS VQEGGKDSCQ GDSGGPLVCN QSLQGIISWG QDPCAITRKP GVYTKVCKYV 250 DWIQETMKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of a novel serine protease, TLSP." Yoshida S., Taniguchi M., Suemoto T., Oka T., He X.P., Shiosaka S. Biochim. Biophys. Acta 1399:225-228(1998) [PubMed: 9765601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hippocampus.
[2]	"A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate." Mitsui S., Yamada T., Okui A., Kominami K., Uemura H., Yamaguchi N. Biochem. Biophys. Res. Commun. 272:205-211(2000) [PubMed: 10872828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Hippocampus and Prostate.
[3]	"Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family." Yousef G.M., Scorilas A., Diamandis E.P. Genomics 63:88-96(2000) [PubMed: 10662548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[4]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed: 11054574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[8]	"Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma." Luo L.Y., Shan S.J., Elliott M.B., Soosaipillai A., Diamandis E.P. Clin. Cancer Res. 12:742-750(2006) [PubMed: 16467084] [Abstract] Cited for: FUNCTION, PROTEOLYTIC PROCESSING, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012917 mRNA. Translation: BAA33404.1. Different initiation. AB013730 mRNA. Translation: BAA88713.1. AB041036 mRNA. Translation: BAA96797.1. AF164623 Genomic DNA. Translation: AAD47815.1. AF243527 Genomic DNA. Translation: AAG33364.1. AY359014 mRNA. Translation: AAQ89373.1. AC011473 Genomic DNA. Translation: AAG23257.1. BC022068 mRNA. Translation: AAH22068.1.
IPI	IPI00002818. IPI00218137.
RefSeq	NP_001129504.1. NP_006844.1. NP_659196.1.
UniGene	Hs.57771
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UBX7.
Protein family/group databases
MEROPS	S01.257.
Proteomic databases
PRIDE	Q9UBX7.
Genome annotation databases
Ensembl	ENST00000319720; ENSP00000324269; ENSG00000167757; Homo sapiens. [Genome view] ENST00000319756; ENSP00000324414; ENSG00000167757; Homo sapiens. [Genome view] ENST00000391803; ENSP00000375679; ENSG00000167757; Homo sapiens. [Genome view] ENST00000391804; ENSP00000375680; ENSG00000167757; Homo sapiens. [Genome view] ENST00000453757; ENSP00000413958; ENSG00000167757; Homo sapiens. [Genome view]
GeneID	11012.
KEGG	hsa:11012.
UCSC	uc002pvc.2. human. uc002pvd.1. human.
Organism-specific databases
CTD	11012.
GeneCards	GC19M056217.
H-InvDB	HIX0015375.
HGNC	HGNC:6359. KLK11.
MIM	604434. gene.
PharmGKB	PA30148.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9UBX7.
HOVERGEN	Q9UBX7.
OMA	NITDTMV.
Gene expression databases
ArrayExpress	Q9UBX7.
Bgee	Q9UBX7.
CleanEx	HS_KLK11.
Genevestigator	Q9UBX7.
GermOnline	ENSG00000167757. Homo sapiens.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	41831.
PMAP-CutDB	Q9UBX7.
SOURCE	Search...

Entry information

Entry name

KLK11_HUMAN

Accession

Primary (citable) accession number: Q9UBX7
Secondary accession number(s): O75837, Q9NS65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 21, 2006
Last modified:	November 3, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.