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Protein

Cathepsin F

Gene

CTSF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Catalytic activityi

The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (k(cat)/K(m)) comparable to that of cathepsin L.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2951 Publication1
Active sitei4311 Publication1
Active sitei451By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciZFISH:HS10762-MONOMER.
BRENDAi3.4.22.41. 2681.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.

Protein family/group databases

MEROPSiC01.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin F (EC:3.4.22.41)
Short name:
CATSF
Gene namesi
Name:CTSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:2531. CTSF.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular space Source: GO_Central
  • extracellular vesicle Source: UniProtKB
  • lysosomal lumen Source: Reactome
  • lysosome Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Ceroid lipofuscinosis, neuronal, 13 (CLN13)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of neuronal ceroid lipofuscinosis characterized by adult onset of progressive cognitive decline and motor dysfunction leading to dementia and often early death. Some patients develop seizures. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material.
See also OMIM:615362
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_070159231Y → C in CLN13. 1 PublicationCorresponds to variant rs143889283dbSNPEnsembl.1
Natural variantiVAR_070160321Q → R in CLN13. 1 PublicationCorresponds to variant rs397514731dbSNPEnsembl.1
Natural variantiVAR_070161458G → A in CLN13. 1 PublicationCorresponds to variant rs397514732dbSNPEnsembl.1
Natural variantiVAR_070162480S → L in CLN13. 1 PublicationCorresponds to variant rs397514733dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation, Neurodegeneration, Neuronal ceroid lipofuscinosis

Organism-specific databases

DisGeNETi8722.
MalaCardsiCTSF.
MIMi615362. phenotype.
OpenTargetsiENSG00000174080.
Orphaneti352709. CLN13 disease.
PharmGKBiPA27031.

Chemistry databases

ChEMBLiCHEMBL2517.
GuidetoPHARMACOLOGYi2347.

Polymorphism and mutation databases

BioMutaiCTSF.
DMDMi12643325.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002620220 – 270Activation peptideAdd BLAST251
ChainiPRO_0000026203271 – 484Cathepsin FAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi292 ↔ 3331 Publication
Disulfide bondi326 ↔ 3661 Publication
Glycosylationi367N-linked (GlcNAc...)1 Publication1
Glycosylationi378N-linked (GlcNAc...)1 Publication1
Disulfide bondi424 ↔ 472By similarity
Glycosylationi440N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ9UBX1.
MaxQBiQ9UBX1.
PaxDbiQ9UBX1.
PeptideAtlasiQ9UBX1.
PRIDEiQ9UBX1.

PTM databases

iPTMnetiQ9UBX1.
PhosphoSitePlusiQ9UBX1.

Expressioni

Tissue specificityi

High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.

Gene expression databases

BgeeiENSG00000174080.
CleanExiHS_CTSF.
ExpressionAtlasiQ9UBX1. baseline and differential.
GenevisibleiQ9UBX1. HS.

Organism-specific databases

HPAiCAB002141.
HPA031431.
HPA055610.

Interactioni

Protein-protein interaction databases

BioGridi114261. 10 interactors.
STRINGi9606.ENSP00000310832.

Chemistry databases

BindingDBiQ9UBX1.

Structurei

Secondary structure

1484
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi277 – 280Combined sources4
Beta strandi291 – 293Combined sources3
Helixi295 – 312Combined sources18
Helixi320 – 326Combined sources7
Beta strandi328 – 330Combined sources3
Helixi338 – 348Combined sources11
Turni354 – 356Combined sources3
Helixi370 – 372Combined sources3
Beta strandi378 – 382Combined sources5
Helixi387 – 397Combined sources11
Beta strandi400 – 404Combined sources5
Helixi407 – 411Combined sources5
Beta strandi414 – 417Combined sources4
Helixi421 – 423Combined sources3
Beta strandi431 – 441Combined sources11
Beta strandi444 – 450Combined sources7
Beta strandi462 – 469Combined sources8
Helixi471 – 473Combined sources3
Turni474 – 477Combined sources4
Beta strandi479 – 482Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5Umodel-A271-484[»]
1M6DX-ray1.70A/B271-484[»]
ProteinModelPortaliQ9UBX1.
SMRiQ9UBX1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBX1.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1542. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiQ9UBX1.
KOiK01373.
OMAiLAPPEWD.
OrthoDBiEOG091G05SD.
PhylomeDBiQ9UBX1.
TreeFamiTF314550.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UBX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPWLQLLSL LGLLPGAVAA PAQPRAASFQ AWGPPSPELL APTRFALEMF
60 70 80 90 100
NRGRAAGTRA VLGLVRGRVR RAGQGSLYSL EATLEEPPCN DPMVCRLPVS
110 120 130 140 150
KKTLLCSFQV LDELGRHVLL RKDCGPVDTK VPGAGEPKSA FTQGSAMISS
160 170 180 190 200
LSQNHPDNRN ETFSSVISLL NEDPLSQDLP VKMASIFKNF VITYNRTYES
210 220 230 240 250
KEEARWRLSV FVNNMVRAQK IQALDRGTAQ YGVTKFSDLT EEEFRTIYLN
260 270 280 290 300
TLLRKEPGNK MKQAKSVGDL APPEWDWRSK GAVTKVKDQG MCGSCWAFSV
310 320 330 340 350
TGNVEGQWFL NQGTLLSLSE QELLDCDKMD KACMGGLPSN AYSAIKNLGG
360 370 380 390 400
LETEDDYSYQ GHMQSCNFSA EKAKVYINDS VELSQNEQKL AAWLAKRGPI
410 420 430 440 450
SVAINAFGMQ FYRHGISRPL RPLCSPWLID HAVLLVGYGN RSDVPFWAIK
460 470 480
NSWGTDWGEK GYYYLHRGSG ACGVNTMASS AVVD
Length:484
Mass (Da):53,366
Last modified:May 1, 2000 - v1
Checksum:i1D5D551B489D822B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti305E → K in AAF13146 (Ref. 5) Curated1
Sequence conflicti442 – 484SDVPF…SAVVD → EFRCLSCIQPGHRQGWDHSI SGPLEGK (PubMed:17974005).CuratedAdd BLAST43

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051513153Q → R.Corresponds to variant rs11550508dbSNPEnsembl.1
Natural variantiVAR_070159231Y → C in CLN13. 1 PublicationCorresponds to variant rs143889283dbSNPEnsembl.1
Natural variantiVAR_070160321Q → R in CLN13. 1 PublicationCorresponds to variant rs397514731dbSNPEnsembl.1
Natural variantiVAR_070161458G → A in CLN13. 1 PublicationCorresponds to variant rs397514732dbSNPEnsembl.1
Natural variantiVAR_070162480S → L in CLN13. 1 PublicationCorresponds to variant rs397514733dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007331 mRNA. Translation: CAB42883.1.
AF088886 mRNA. Translation: AAD26616.2.
AF132894 Genomic DNA. Translation: AAD41790.1.
AF136279 mRNA. Translation: AAF13146.1.
AF071748 mRNA. Translation: AAC78838.1.
AF071749 mRNA. Translation: AAC78839.1.
AK313657 mRNA. Translation: BAG36411.1.
BC011682 mRNA. Translation: AAH11682.1.
BC036451 mRNA. Translation: AAH36451.1.
AL137742 mRNA. Translation: CAB70900.1.
CCDSiCCDS8144.1.
RefSeqiNP_003784.2. NM_003793.3.
UniGeneiHs.11590.

Genome annotation databases

EnsembliENST00000310325; ENSP00000310832; ENSG00000174080.
GeneIDi8722.
KEGGihsa:8722.
UCSCiuc001oip.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007331 mRNA. Translation: CAB42883.1.
AF088886 mRNA. Translation: AAD26616.2.
AF132894 Genomic DNA. Translation: AAD41790.1.
AF136279 mRNA. Translation: AAF13146.1.
AF071748 mRNA. Translation: AAC78838.1.
AF071749 mRNA. Translation: AAC78839.1.
AK313657 mRNA. Translation: BAG36411.1.
BC011682 mRNA. Translation: AAH11682.1.
BC036451 mRNA. Translation: AAH36451.1.
AL137742 mRNA. Translation: CAB70900.1.
CCDSiCCDS8144.1.
RefSeqiNP_003784.2. NM_003793.3.
UniGeneiHs.11590.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5Umodel-A271-484[»]
1M6DX-ray1.70A/B271-484[»]
ProteinModelPortaliQ9UBX1.
SMRiQ9UBX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114261. 10 interactors.
STRINGi9606.ENSP00000310832.

Chemistry databases

BindingDBiQ9UBX1.
ChEMBLiCHEMBL2517.
GuidetoPHARMACOLOGYi2347.

Protein family/group databases

MEROPSiC01.018.

PTM databases

iPTMnetiQ9UBX1.
PhosphoSitePlusiQ9UBX1.

Polymorphism and mutation databases

BioMutaiCTSF.
DMDMi12643325.

Proteomic databases

EPDiQ9UBX1.
MaxQBiQ9UBX1.
PaxDbiQ9UBX1.
PeptideAtlasiQ9UBX1.
PRIDEiQ9UBX1.

Protocols and materials databases

DNASUi8722.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310325; ENSP00000310832; ENSG00000174080.
GeneIDi8722.
KEGGihsa:8722.
UCSCiuc001oip.4. human.

Organism-specific databases

CTDi8722.
DisGeNETi8722.
GeneCardsiCTSF.
GeneReviewsiCTSF.
HGNCiHGNC:2531. CTSF.
HPAiCAB002141.
HPA031431.
HPA055610.
MalaCardsiCTSF.
MIMi603539. gene.
615362. phenotype.
neXtProtiNX_Q9UBX1.
OpenTargetsiENSG00000174080.
Orphaneti352709. CLN13 disease.
PharmGKBiPA27031.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1542. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiQ9UBX1.
KOiK01373.
OMAiLAPPEWD.
OrthoDBiEOG091G05SD.
PhylomeDBiQ9UBX1.
TreeFamiTF314550.

Enzyme and pathway databases

BioCyciZFISH:HS10762-MONOMER.
BRENDAi3.4.22.41. 2681.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.

Miscellaneous databases

ChiTaRSiCTSF. human.
EvolutionaryTraceiQ9UBX1.
GeneWikiiCathepsin_F.
GenomeRNAii8722.
PROiQ9UBX1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174080.
CleanExiHS_CTSF.
ExpressionAtlasiQ9UBX1. baseline and differential.
GenevisibleiQ9UBX1. HS.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATF_HUMAN
AccessioniPrimary (citable) accession number: Q9UBX1
Secondary accession number(s): B2R964
, O95240, Q9NSU4, Q9UKQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.