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Protein

Bridging integrator 2

Gene

BIN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.1 Publication

GO - Molecular functioni

  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • cell chemotaxis Source: UniProtKB
  • membrane tubulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • podosome assembly Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110934-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Bridging integrator 2
Alternative name(s):
Breast cancer-associated protein 1
Gene namesi
Name:BIN2
Synonyms:BRAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:1053. BIN2.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • phagocytic cup Source: UniProtKB-SubCell
  • podosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13F → A: Strongly reduces binding to membranes. 1 Publication1
Mutagenesisi21F → A: Strongly reduces binding to membranes. 1 Publication1
Mutagenesisi81V → R: Abolishes dimerization and membrane binding; when associated with E-214. 1 Publication1
Mutagenesisi214S → E: Abolishes dimerization and membrane binding; when associated with R-81. 1 Publication1

Organism-specific databases

DisGeNETi51411.
PharmGKBiPA25356.

Polymorphism and mutation databases

BioMutaiBIN2.
DMDMi143811367.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002561401 – 565Bridging integrator 2Add BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273PhosphoserineCombined sources1
Modified residuei357PhosphoserineCombined sources1
Modified residuei383PhosphoserineBy similarity1
Modified residuei395PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei438PhosphothreonineCombined sources1
Modified residuei440PhosphoserineBy similarity1
Modified residuei444PhosphoserineCombined sources1
Modified residuei447PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1
Modified residuei462PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UBW5.
PaxDbiQ9UBW5.
PeptideAtlasiQ9UBW5.
PRIDEiQ9UBW5.

2D gel databases

OGPiQ9UBW5.

PTM databases

iPTMnetiQ9UBW5.
PhosphoSitePlusiQ9UBW5.
SwissPalmiQ9UBW5.

Expressioni

Tissue specificityi

Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues.2 Publications

Inductioni

Up-regulated during monocytic differentiation.1 Publication

Gene expression databases

BgeeiENSG00000110934.
CleanExiHS_BIN2.

Organism-specific databases

HPAiHPA038666.
HPA038667.

Interactioni

Subunit structurei

Homodimer. Interacts with BIN1. Interacts with ARHGEF6 (via SH3 domain), ARHGEF7 (via SH3 domain), SH3GL1, SH3GL2 and SH3GL3. Identified in a complex with ARHGEF6 and GIT2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BIN1O004992EBI-2042570,EBI-719094

Protein-protein interaction databases

BioGridi119528. 7 interactors.
IntActiQ9UBW5. 6 interactors.
STRINGi9606.ENSP00000267012.

Structurei

Secondary structure

1565
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 31Combined sources16
Helixi41 – 88Combined sources48
Helixi96 – 120Combined sources25
Helixi122 – 130Combined sources9
Helixi132 – 160Combined sources29
Beta strandi161 – 164Combined sources4
Helixi167 – 200Combined sources34
Helixi202 – 241Combined sources40

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AVMX-ray1.91A11-245[»]
4I1QX-ray2.53A/B20-238[»]
ProteinModelPortaliQ9UBW5.
SMRiQ9UBW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 244BARPROSITE-ProRule annotationAdd BLAST217

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili304 – 329Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi253 – 256Poly-Ser4
Compositional biasi413 – 479Pro-richAdd BLAST67

Domaini

The BAR domain mediates dimerization and interaction with membranes enriched in phosphatidylinositides.1 Publication

Sequence similaritiesi

Contains 1 BAR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ITR5. Eukaryota.
ENOG410Y56Y. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiQ9UBW5.
KOiK20119.
PhylomeDBiQ9UBW5.
TreeFamiTF313542.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR004148. BAR_dom.
IPR028827. BIN2.
[Graphical view]
PANTHERiPTHR10663:SF166. PTHR10663:SF166. 1 hit.
PfamiPF03114. BAR. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
SMARTiSM00721. BAR. 1 hit.
[Graphical view]
PROSITEiPS51021. BAR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF
60 70 80 90 100
YQQQAEGHKL YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK
110 120 130 140 150
AIVWNNDLLW EDYEEKLADQ AVRTMEIYVA QFSEIKERIA KRGRKLVDYD
160 170 180 190 200
SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA QTVFEDLNQE LLEELPILYN
210 220 230 240 250
SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK QHSNKVFVVK
260 270 280 290 300
GLSSSSRRSL VISPPVRTAT VSSPLTSPTS PSTLSLKSES ESVSATEDLA
310 320 330 340 350
PDAAQGEDNS EIKELLEEEE IEKEGSEASS SEEDEPLPAC NGPAQAQPSP
360 370 380 390 400
TTERAKSQEE VLPSSTTPSP GGALSPSGQP SSSATEVVLR TRTASEGSEQ
410 420 430 440 450
PKKRASIQRT SAPPSRPPPP RATASPRPSS GNIPSSPTAS GGGSPTSPRA
460 470 480 490 500
SLGTGTASPR TSLEVSPNPE PPEKPVRTPE AKENENIHNQ NPEELCTSPT
510 520 530 540 550
LMTSQVASEP GEAKKMEDKE KDNKLISANS SEGQDQLQVS MVPENNNLTA
560
PEPQEEVSTS ENPQL
Length:565
Mass (Da):61,874
Last modified:April 3, 2007 - v3
Checksum:iB1D1FB89A97E9E43
GO
Isoform 2 (identifier: Q9UBW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-136: Missing.

Note: No experimental confirmation available.
Show »
Length:533
Mass (Da):58,030
Checksum:iE549F6F418F01E02
GO

Sequence cautioni

The sequence BAA91376 differs from that shown. Reason: Frameshift at position 156.Curated
The sequence BAA91376 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163Missing in AAD54227 (PubMed:10903846).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02888348S → N.1 PublicationCorresponds to variant rs7312857dbSNPEnsembl.1
Natural variantiVAR_028884529N → D.4 PublicationsCorresponds to variant rs7954976dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021328105 – 136Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146531 mRNA. Translation: AAD54227.1.
AB032698 mRNA. Translation: BAA88108.1.
AB032710 Genomic DNA. Translation: BAA88125.1.
AK000783 mRNA. Translation: BAA91376.1. Sequence problems.
AC046135 Genomic DNA. No translation available.
BC047686 mRNA. Translation: AAH47686.1.
RefSeqiNP_001276936.1. NM_001290007.1.
NP_001276937.1. NM_001290008.1.
NP_001276938.1. NM_001290009.1.
NP_057377.3. NM_016293.3.
UniGeneiHs.14770.

Genome annotation databases

EnsembliENST00000267012; ENSP00000267012; ENSG00000110934.
GeneIDi51411.
KEGGihsa:51411.
UCSCiuc058ocp.1. human. [Q9UBW5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146531 mRNA. Translation: AAD54227.1.
AB032698 mRNA. Translation: BAA88108.1.
AB032710 Genomic DNA. Translation: BAA88125.1.
AK000783 mRNA. Translation: BAA91376.1. Sequence problems.
AC046135 Genomic DNA. No translation available.
BC047686 mRNA. Translation: AAH47686.1.
RefSeqiNP_001276936.1. NM_001290007.1.
NP_001276937.1. NM_001290008.1.
NP_001276938.1. NM_001290009.1.
NP_057377.3. NM_016293.3.
UniGeneiHs.14770.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AVMX-ray1.91A11-245[»]
4I1QX-ray2.53A/B20-238[»]
ProteinModelPortaliQ9UBW5.
SMRiQ9UBW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119528. 7 interactors.
IntActiQ9UBW5. 6 interactors.
STRINGi9606.ENSP00000267012.

PTM databases

iPTMnetiQ9UBW5.
PhosphoSitePlusiQ9UBW5.
SwissPalmiQ9UBW5.

Polymorphism and mutation databases

BioMutaiBIN2.
DMDMi143811367.

2D gel databases

OGPiQ9UBW5.

Proteomic databases

EPDiQ9UBW5.
PaxDbiQ9UBW5.
PeptideAtlasiQ9UBW5.
PRIDEiQ9UBW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267012; ENSP00000267012; ENSG00000110934.
GeneIDi51411.
KEGGihsa:51411.
UCSCiuc058ocp.1. human. [Q9UBW5-1]

Organism-specific databases

CTDi51411.
DisGeNETi51411.
GeneCardsiBIN2.
H-InvDBHIX0201823.
HGNCiHGNC:1053. BIN2.
HPAiHPA038666.
HPA038667.
MIMi605936. gene.
neXtProtiNX_Q9UBW5.
PharmGKBiPA25356.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITR5. Eukaryota.
ENOG410Y56Y. LUCA.
HOGENOMiHOG000252987.
HOVERGENiHBG004224.
InParanoidiQ9UBW5.
KOiK20119.
PhylomeDBiQ9UBW5.
TreeFamiTF313542.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110934-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiBIN2. human.
GenomeRNAii51411.
PROiQ9UBW5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110934.
CleanExiHS_BIN2.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR004148. BAR_dom.
IPR028827. BIN2.
[Graphical view]
PANTHERiPTHR10663:SF166. PTHR10663:SF166. 1 hit.
PfamiPF03114. BAR. 1 hit.
[Graphical view]
PRINTSiPR01251. AMPHIPHYSIN.
SMARTiSM00721. BAR. 1 hit.
[Graphical view]
PROSITEiPS51021. BAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIN2_HUMAN
AccessioniPrimary (citable) accession number: Q9UBW5
Secondary accession number(s): Q86VV0, Q9NWK4, Q9UKN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 3, 2007
Last modified: November 30, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.