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Q9UBW5 (BIN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bridging integrator 2
Alternative name(s):
Breast cancer-associated protein 1
Gene names
Name:BIN2
Synonyms:BRAP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis. Ref.8

Subunit structure

Homodimer. Interacts with BIN1. Interacts with ARHGEF6 (via SH3 domain), ARHGEF7 (via SH3 domain), SH3GL1, SH3GL2 and SH3GL3. Identified in a complex with ARHGEF6 and GIT2. Ref.1 Ref.8

Subcellular location

Cytoplasm. Cell projectionpodosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcell cortex. Cell projectionphagocytic cup. Note: Associates with membranes enriched in phosphoinositides. Detected in the actin-rich cell cortex at the leading edge of migrating cells. Detected at podosomes, at an actin-rich ring-like structure. Ref.1 Ref.8

Tissue specificity

Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues. Ref.1 Ref.8

Induction

Up-regulated during monocytic differentiation. Ref.1

Domain

The BAR domain mediates dimerization and interaction with membranes enriched in phosphatidylinositides. Ref.8

Sequence similarities

Contains 1 BAR domain.

Sequence caution

The sequence BAA91376.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAA91376.1 differs from that shown. Reason: Frameshift at position 156.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BIN1O004992EBI-2042570,EBI-719094

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UBW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UBW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     105-136: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565Bridging integrator 2
PRO_0000256140

Regions

Domain28 – 244217BAR
Coiled coil304 – 32926 Potential
Compositional bias253 – 2564Poly-Ser
Compositional bias413 – 47967Pro-rich

Amino acid modifications

Modified residue2731Phosphoserine Ref.7
Modified residue3571Phosphoserine Ref.7
Modified residue4361Phosphoserine Ref.7
Modified residue4381Phosphothreonine Ref.7
Modified residue4441Phosphoserine Ref.7
Modified residue4471Phosphoserine Ref.7
Modified residue4581Phosphoserine Ref.7

Natural variations

Alternative sequence105 – 13632Missing in isoform 2.
VSP_021328
Natural variant481S → N. Ref.2
Corresponds to variant rs7312857 [ dbSNP | Ensembl ].
VAR_028883
Natural variant5291N → D. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs7954976 [ dbSNP | Ensembl ].
VAR_028884

Experimental info

Mutagenesis131F → A: Strongly reduces binding to membranes. Ref.8
Mutagenesis211F → A: Strongly reduces binding to membranes. Ref.8
Mutagenesis811V → R: Abolishes dimerization and membrane binding; when associated with E-214. Ref.8
Mutagenesis2141S → E: Abolishes dimerization and membrane binding; when associated with R-81. Ref.8
Sequence conflict1631Missing in AAD54227. Ref.1

Secondary structure

................ 565
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 3, 2007. Version 3.
Checksum: B1D1FB89A97E9E43

FASTA56561,874
        10         20         30         40         50         60 
MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF YQQQAEGHKL 

        70         80         90        100        110        120 
YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK AIVWNNDLLW EDYEEKLADQ 

       130        140        150        160        170        180 
AVRTMEIYVA QFSEIKERIA KRGRKLVDYD SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA 

       190        200        210        220        230        240 
QTVFEDLNQE LLEELPILYN SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK 

       250        260        270        280        290        300 
QHSNKVFVVK GLSSSSRRSL VISPPVRTAT VSSPLTSPTS PSTLSLKSES ESVSATEDLA 

       310        320        330        340        350        360 
PDAAQGEDNS EIKELLEEEE IEKEGSEASS SEEDEPLPAC NGPAQAQPSP TTERAKSQEE 

       370        380        390        400        410        420 
VLPSSTTPSP GGALSPSGQP SSSATEVVLR TRTASEGSEQ PKKRASIQRT SAPPSRPPPP 

       430        440        450        460        470        480 
RATASPRPSS GNIPSSPTAS GGGSPTSPRA SLGTGTASPR TSLEVSPNPE PPEKPVRTPE 

       490        500        510        520        530        540 
AKENENIHNQ NPEELCTSPT LMTSQVASEP GEAKKMEDKE KDNKLISANS SEGQDQLQVS 

       550        560 
MVPENNNLTA PEPQEEVSTS ENPQL 

« Hide

Isoform 2 [UniParc].

Checksum: E549F6F418F01E02
Show »

FASTA53358,030

References

« Hide 'large scale' references
[1]"Bin2, a functionally nonredundant member of the BAR adaptor gene family."
Ge K., Prendergast G.C.
Genomics 67:210-220(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ASP-529, INDUCTION, INTERACTION WITH BIN2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Genomic structure and chromosome location of the BRAP1 gene."
Miki Y., Saito H.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), VARIANTS ASN-48 AND ASP-529.
Tissue: Mammary gland.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ASP-529.
Tissue: Colon.
[4]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-529.
Tissue: Ovary.
[6]"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.
J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: T-cell.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273; SER-357; SER-436; THR-438; SER-444; SER-447 AND SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Bin2 is a membrane sculpting N-BAR protein that influences leucocyte podosomes, motility and phagocytosis."
Sanchez-Barrena M.J., Vallis Y., Clatworthy M.R., Doherty G.J., Veprintsev D.B., Evans P.R., McMahon H.T.
PLoS ONE 7:E52401-E52401(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.53 ANGSTROMS) OF 1-238, FUNCTION, DOMAIN, SUBUNIT, INTERACTION WITH ARHGEF6; ARHGEF7; SH3GL1; SH3GL2; SH3GL3, IDENTIFICATION IN A COMPLEX WITH GIT2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF PHE-13; PHE-21; VAL-81 AND SER-214.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF146531 mRNA. Translation: AAD54227.1.
AB032698 mRNA. Translation: BAA88108.1.
AB032710 Genomic DNA. Translation: BAA88125.1.
AK000783 mRNA. Translation: BAA91376.1. Sequence problems.
AC046135 Genomic DNA. No translation available.
BC047686 mRNA. Translation: AAH47686.1.
RefSeqNP_001276936.1. NM_001290007.1.
NP_001276937.1. NM_001290008.1.
NP_001276938.1. NM_001290009.1.
NP_057377.3. NM_016293.3.
UniGeneHs.14770.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AVMX-ray1.91A11-245[»]
4I1QX-ray2.53A/B20-238[»]
ProteinModelPortalQ9UBW5.
SMRQ9UBW5. Positions 15-244.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119528. 5 interactions.
IntActQ9UBW5. 3 interactions.
STRING9606.ENSP00000267012.

PTM databases

PhosphoSiteQ9UBW5.

Polymorphism databases

DMDM143811367.

2D gel databases

OGPQ9UBW5.

Proteomic databases

MaxQBQ9UBW5.
PaxDbQ9UBW5.
PRIDEQ9UBW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000267012; ENSP00000267012; ENSG00000110934. [Q9UBW5-1]
ENST00000452142; ENSP00000410217; ENSG00000110934. [Q9UBW5-2]
GeneID51411.
KEGGhsa:51411.
UCSCuc001ryg.3. human. [Q9UBW5-1]
uc009zlz.3. human. [Q9UBW5-2]

Organism-specific databases

GeneCardsGC12M051697.
H-InvDBHIX0201823.
HGNCHGNC:1053. BIN2.
HPAHPA038666.
HPA038667.
MIM605936. gene.
neXtProtNX_Q9UBW5.
PharmGKBPA25356.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG264615.
HOGENOMHOG000252987.
HOVERGENHBG004224.
InParanoidQ9UBW5.
OMAQEEVLPC.
OrthoDBEOG7XWPND.
PhylomeDBQ9UBW5.
TreeFamTF313542.

Gene expression databases

ArrayExpressQ9UBW5.
BgeeQ9UBW5.
CleanExHS_BIN2.
GenevestigatorQ9UBW5.

Family and domain databases

Gene3D1.20.1270.60. 1 hit.
InterProIPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR004148. BAR_dom.
IPR028827. BIN2.
[Graphical view]
PANTHERPTHR10321:SF11. PTHR10321:SF11. 1 hit.
PfamPF03114. BAR. 1 hit.
[Graphical view]
PRINTSPR01251. AMPHIPHYSIN.
SMARTSM00721. BAR. 1 hit.
[Graphical view]
PROSITEPS51021. BAR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBIN2. human.
GenomeRNAi51411.
NextBio54957.
PROQ9UBW5.
SOURCESearch...

Entry information

Entry nameBIN2_HUMAN
AccessionPrimary (citable) accession number: Q9UBW5
Secondary accession number(s): Q86VV0, Q9NWK4, Q9UKN4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 3, 2007
Last modified: July 9, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM