Q9UBV8 (PEF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peflin

Alternative name(s):
PEF protein with a long N-terminal hydrophobic domain
Penta-EF hand domain-containing protein 1

Gene names

Name:	PEF1
Synonyms:	ABP32
ORF Names:	UNQ1845/PRO3573

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterodimer; heterodimerizes with PDCD6. Dissociates from PDCD6 in presence of Ca2+.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Membrane-associated in the presence of Ca2+. Ref.7
Sequence similarities	Contains 5 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to calcium ion Inferred from physical interaction Ref.7. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay Ref.7. Source: UniProtKB membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Traceable author statement. Source: ProtInc protein heterodimerization activity Inferred from physical interaction Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAZAP2	Q15038	3	EBI-724639,EBI-724310
KLHL12	Q53G59	2	EBI-724639,EBI-740929

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Peflin		PRO_0000247045
Regions
	Repeat	21 – 29	9	1
	Repeat	31 – 39	9	2
	Repeat	41 – 49	9	3
	Repeat	50 – 58	9	4
	Repeat	59 – 67	9	5
	Repeat	76 – 84	9	6
	Repeat	85 – 92	8	7
	Repeat	93 – 100	8	8
	Repeat	101 – 109	9	9
	Domain	114 – 149	36	EF-hand 1
	Domain	155 – 183	29	EF-hand 2
	Domain	181 – 216	36	EF-hand 3
	Domain	217 – 253	37	EF-hand 4
	Domain	254 – 283	30	EF-hand 5
	Calcium binding	127 – 138	12	1 Potential
	Calcium binding	194 – 205	12	2 Potential
	Region	21 – 109	89	9 X 9 AA approximate tandem repeat of [AP]-P-G-G-P-Y-G-G-P-P
	Region	204 – 284	81	Required for interaction with PDCD6

Sequences

	Sequence		Length	Mass (Da)
	Q9UBV8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2E9AA5750CB7A68A	FASTA	284	30,381
	10 20 30 40 50 60 MASYPYRQGC PGAAGQAPGA PPGSYYPGPP NSGGQYGSGL PPGGGYGGPA PGGPYGPPAG 70 80 90 100 110 120 GGPYGHPNPG MFPSGTPGGP YGGAAPGGPY GQPPPSSYGA QQPGLYGQGG APPNVDPEAY 130 140 150 160 170 180 SWFQSVDSDH SGYISMKELK QALVNCNWSS FNDETCLMMI NMFDKTKSGR IDVYGFSALW 190 200 210 220 230 240 KFIQQWKNLF QQYDRDRSGS ISYTELQQAL SQMGYNLSPQ FTQLLVSRYC PRSANPAMQL 250 260 270 280 DRFIQVCTQL QVLTEAFREK DTAVQGNIRL SFEDFVTMTA SRML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peflin, a novel member of the five-EF-hand-protein family, is similar to the apoptosis-linked gene 2 (ALG-2) protein but possesses nonapeptide repeats in the N-terminal hydrophobic region." Kitaura Y., Watanabe M., Satoh H., Kawai T., Hitomi K., Maki M. Biochem. Biophys. Res. Commun. 263:68-75(1999) [PubMed: 10486255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A putative calcium binding protein that has five EF-hand motiefs." Shibata M., Noguchi J. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate and Skin.
[7]	"Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner." Kitaura Y., Matsumoto S., Satoh H., Hitomi K., Maki M. J. Biol. Chem. 276:14053-14058(2001) [PubMed: 11278427] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PDCD6.
[8]	"Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions." Kitaura Y., Satoh H., Takahashi H., Shibata H., Maki M. Arch. Biochem. Biophys. 399:12-18(2002) [PubMed: 11883899] [Abstract] Cited for: INTERACTION WITH PDCD6.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026628 mRNA. Translation: BAA85163.1. AB018357 mRNA. Translation: BAA84922.1. AY359011 mRNA. Translation: AAQ89370.1. AK001420 mRNA. Translation: BAA91680.1. CR542139 mRNA. Translation: CAG46936.1. BC002773 mRNA. Translation: AAH02773.1. BC012561 mRNA. Translation: AAH12561.1.
IPI	IPI00018235.
RefSeq	NP_036524.1. NM_012392.3.
UniGene	Hs.470417.
3D structure databases
ProteinModelPortal	Q9UBV8.
SMR	Q9UBV8. Positions 115-279.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UBV8. 10 interactions.
STRING	Q9UBV8.
PTM databases
PhosphoSite	Q9UBV8.
Polymorphism databases
DMDM	74761895.
2D gel databases
REPRODUCTION-2DPAGE	IPI00018235.
Proteomic databases
PRIDE	Q9UBV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373703; ENSP00000362807; ENSG00000162517.
GeneID	553115.
KEGG	hsa:553115.
UCSC	uc001btg.1. human.
Organism-specific databases
CTD	553115.
GeneCards	GC01M032095.
H-InvDB	HIX0000358.
HGNC	HGNC:30009. PEF1.
MIM	610033. gene.
neXtProt	NX_Q9UBV8.
PharmGKB	PA142671184.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18092.
GeneTree	ENSGT00580000081418.
HOGENOM	HBG747897.
HOVERGEN	HBG004492.
InParanoid	Q9UBV8.
OMA	GAQHPGP.
OrthoDB	EOG45B1GP.
PhylomeDB	Q9UBV8.
Gene expression databases
ArrayExpress	Q9UBV8.
Bgee	Q9UBV8.
CleanEx	HS_PEF1.
Genevestigator	Q9UBV8.
GermOnline	ENSG00000162517. Homo sapiens.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	112375.
SOURCE	Search...

Entry information

Entry name

PEF1_HUMAN

Accession

Primary (citable) accession number: Q9UBV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.