Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9UBV7

- B4GT7_HUMAN

UniProt

Q9UBV7 - B4GT7_HUMAN

Protein

Beta-1,4-galactosyltransferase 7

Gene

B4GALT7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein].1 Publication

    Cofactori

    Manganese.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi165 – 1651Manganese
    Binding sitei194 – 1941UDP-alpha-D-galactose1 Publication
    Binding sitei224 – 2241UDP-alpha-D-galactose1 Publication
    Metal bindingi257 – 2571Manganese; via tele nitrogen
    Binding sitei266 – 2661UDP-alpha-D-galactose1 Publication

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB
    2. galactosyltransferase activity Source: UniProtKB
    3. manganese ion binding Source: UniProtKB
    4. xylosylprotein 4-beta-galactosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. cellular protein modification process Source: ProtInc
    3. chondroitin sulfate metabolic process Source: Reactome
    4. extracellular fibril organization Source: UniProtKB
    5. glycosaminoglycan biosynthetic process Source: UniProtKB
    6. glycosaminoglycan metabolic process Source: Reactome
    7. negative regulation of fibroblast proliferation Source: UniProtKB
    8. protein N-linked glycosylation Source: UniProtKB
    9. proteoglycan metabolic process Source: UniProtKB
    10. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS00459-MONOMER.
    ReactomeiREACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 7 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 7
    Short name:
    Beta4Gal-T7
    Short name:
    b4Gal-T7
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7
    Including the following 1 domains:
    Xylosylprotein 4-beta-galactosyltransferase (EC:2.4.1.133)
    Alternative name(s):
    Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I
    UDP-galactose:beta-xylose beta-1,4-galactosyltransferase
    XGPT
    XGalT-1
    Xylosylprotein beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4GALT7
    Synonyms:XGALT1
    ORF Names:UNQ748/PRO1478
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:930. B4GALT7.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB
    2. Golgi cisterna membrane Source: UniProtKB-SubCell
    3. Golgi membrane Source: Reactome
    4. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Ehlers-Danlos syndrome, progeroid type, 1 (EDSP1) [MIM:130070]: A variant form of Ehlers-Danlos syndrome characterized by progeroid facies, mild mental retardation, short stature, skin hyperextensibility, moderate skin fragility, joint hypermobility principally in digits.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti186 – 1861A → D in EDSP1. 1 Publication
    VAR_010293
    Natural varianti206 – 2061L → P in EDSP1. 1 Publication
    VAR_010294

    Keywords - Diseasei

    Disease mutation, Ehlers-Danlos syndrome

    Organism-specific databases

    MIMi130070. phenotype.
    Orphaneti75496. Ehlers-Danlos syndrome, progeroid type.
    PharmGKBiPA25229.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Beta-1,4-galactosyltransferase 7PRO_0000080550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi316 ↔ 324

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9UBV7.
    PaxDbiQ9UBV7.
    PRIDEiQ9UBV7.

    PTM databases

    PhosphoSiteiQ9UBV7.

    Expressioni

    Tissue specificityi

    High expression in heart, pancreas and liver, medium in placenta and kidney, low in brain, skeletal muscle and lung.

    Gene expression databases

    ArrayExpressiQ9UBV7.
    BgeeiQ9UBV7.
    CleanExiHS_B4GALT7.
    GenevestigatoriQ9UBV7.

    Organism-specific databases

    HPAiHPA042330.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000029410.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi82 – 843
    Helixi88 – 903
    Beta strandi95 – 1039
    Helixi105 – 12117
    Beta strandi126 – 1338
    Beta strandi135 – 1373
    Helixi141 – 15111
    Beta strandi158 – 1625
    Beta strandi166 – 1683
    Beta strandi183 – 1864
    Turni188 – 1903
    Beta strandi191 – 1933
    Beta strandi202 – 2076
    Helixi208 – 2136
    Beta strandi223 – 2264
    Helixi227 – 23711
    Helixi251 – 2533
    Beta strandi254 – 2574
    Turni261 – 2633
    Helixi274 – 2774
    Turni287 – 2893
    Beta strandi292 – 30211
    Beta strandi305 – 31410
    Turni318 – 3203
    Helixi322 – 3254

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IRPX-ray2.10A/B81-327[»]
    4IRQX-ray2.30A/B/C/D81-327[»]
    ProteinModelPortaliQ9UBV7.
    SMRiQ9UBV7. Positions 81-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3030CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini52 – 327276LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni100 – 1045UDP-alpha-D-galactose binding
    Regioni139 – 1413UDP-alpha-D-galactose bindingBy similarity
    Regioni164 – 1652UDP-alpha-D-galactose binding
    Regioni226 – 2294N-acetyl-D-glucosamine bindingBy similarity
    Regioni257 – 2593UDP-alpha-D-galactose binding

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305756.
    HOGENOMiHOG000286021.
    HOVERGENiHBG050654.
    InParanoidiQ9UBV7.
    KOiK00733.
    OMAiFRFNRAS.
    OrthoDBiEOG7C5M8P.
    PhylomeDBiQ9UBV7.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9UBV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPSRRKAAQ LPWEDGRSGL LSGGLPRKCS VFHLFVACLS LGFFSLLWLQ    50
    LSCSGDVARA VRGQGQETSG PPRACPPEPP PEHWEEDASW GPHRLAVLVP 100
    FRERFEELLV FVPHMRRFLS RKKIRHHIYV LNQVDHFRFN RAALINVGFL 150
    ESSNSTDYIA MHDVDLLPLN EELDYGFPEA GPFHVASPEL HPLYHYKTYV 200
    GGILLLSKQH YRLCNGMSNR FWGWGREDDE FYRRIKGAGL QLFRPSGITT 250
    GYKTFRHLHD PAWRKRDQKR IAAQKQEQFK VDREGGLNTV KYHVASRTAL 300
    SVGGAPCTVL NIMLDCDKTA TPWCTFS 327
    Length:327
    Mass (Da):37,406
    Last modified:May 1, 2000 - v1
    Checksum:i2EDF51A2F8143135
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti147 – 1471V → L in AAF22225. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti186 – 1861A → D in EDSP1. 1 Publication
    VAR_010293
    Natural varianti206 – 2061L → P in EDSP1. 1 Publication
    VAR_010294

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005382 mRNA. Translation: CAB56424.1.
    AB028600 mRNA. Translation: BAA83414.1.
    AF142675 mRNA. Translation: AAF22225.1.
    AY358578 mRNA. Translation: AAQ88941.1.
    AK023506 mRNA. Translation: BAG51201.1.
    CH471195 Genomic DNA. Translation: EAW84965.1.
    BC007317 mRNA. Translation: AAH07317.1.
    BC062983 mRNA. Translation: AAH62983.1.
    BC072403 mRNA. Translation: AAH72403.1.
    CCDSiCCDS4429.1.
    RefSeqiNP_009186.1. NM_007255.2.
    UniGeneiHs.455109.

    Genome annotation databases

    EnsembliENST00000029410; ENSP00000029410; ENSG00000027847.
    GeneIDi11285.
    KEGGihsa:11285.
    UCSCiuc003mhy.3. human.

    Polymorphism databases

    DMDMi13123990.

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Beta-1,4-galactosyltransferase 7

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005382 mRNA. Translation: CAB56424.1 .
    AB028600 mRNA. Translation: BAA83414.1 .
    AF142675 mRNA. Translation: AAF22225.1 .
    AY358578 mRNA. Translation: AAQ88941.1 .
    AK023506 mRNA. Translation: BAG51201.1 .
    CH471195 Genomic DNA. Translation: EAW84965.1 .
    BC007317 mRNA. Translation: AAH07317.1 .
    BC062983 mRNA. Translation: AAH62983.1 .
    BC072403 mRNA. Translation: AAH72403.1 .
    CCDSi CCDS4429.1.
    RefSeqi NP_009186.1. NM_007255.2.
    UniGenei Hs.455109.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IRP X-ray 2.10 A/B 81-327 [» ]
    4IRQ X-ray 2.30 A/B/C/D 81-327 [» ]
    ProteinModelPortali Q9UBV7.
    SMRi Q9UBV7. Positions 81-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000029410.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q9UBV7.

    Polymorphism databases

    DMDMi 13123990.

    Proteomic databases

    MaxQBi Q9UBV7.
    PaxDbi Q9UBV7.
    PRIDEi Q9UBV7.

    Protocols and materials databases

    DNASUi 11285.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000029410 ; ENSP00000029410 ; ENSG00000027847 .
    GeneIDi 11285.
    KEGGi hsa:11285.
    UCSCi uc003mhy.3. human.

    Organism-specific databases

    CTDi 11285.
    GeneCardsi GC05P177027.
    HGNCi HGNC:930. B4GALT7.
    HPAi HPA042330.
    MIMi 130070. phenotype.
    604327. gene.
    neXtProti NX_Q9UBV7.
    Orphaneti 75496. Ehlers-Danlos syndrome, progeroid type.
    PharmGKBi PA25229.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG305756.
    HOGENOMi HOG000286021.
    HOVERGENi HBG050654.
    InParanoidi Q9UBV7.
    KOi K00733.
    OMAi FRFNRAS.
    OrthoDBi EOG7C5M8P.
    PhylomeDBi Q9UBV7.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:HS00459-MONOMER.
    Reactomei REACT_121408. A tetrasaccharide linker sequence is required for GAG synthesis.

    Miscellaneous databases

    GeneWikii B4GALT7.
    GenomeRNAii 11285.
    NextBioi 42963.
    PROi Q9UBV7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UBV7.
    Bgeei Q9UBV7.
    CleanExi HS_B4GALT7.
    Genevestigatori Q9UBV7.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I. A seventh member of the human beta4-galactosyltransferase gene family."
      Almeida R., Levery S.B., Mandel U., Kresse H., Schwientek T., Bennett E.P., Clausen H.
      J. Biol. Chem. 274:26165-26171(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Human homolog of Caenorhabditis elegans sqv-3 gene is galactosyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans."
      Okajima T., Yoshida K., Kondo T., Furukawa K.
      J. Biol. Chem. 274:22915-22918(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Melanoma.
    3. "Human beta-1,4-galactosyltransferase VII."
      Lo N.-W., Shaper N.L., Shaper J.H.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas and Skin.
    8. "Crystal structures of beta-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate Binding."
      Tsutsui Y., Ramakrishnan B., Qasba P.K.
      J. Biol. Chem. 288:31963-31970(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 81-327 IN COMPLEX WITH UDP AND MANGANESE IONS, CATALYTIC ACTIVITY, FUNCTION, COFACTOR.
    9. "Molecular basis for the progeroid variant of Ehlers-Danlos syndrome. Identification and characterization of two mutations in galactosyltransferase I gene."
      Okajima T., Fukumoto S., Furukawa K., Urano T.
      J. Biol. Chem. 274:28841-28844(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS EDSP1 ASP-186 AND PRO-206.
    10. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
      Amado M., Almeida R., Schwientek T., Clausen H.
      Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiB4GT7_HUMAN
    AccessioniPrimary (citable) accession number: Q9UBV7
    Secondary accession number(s): B3KN39, Q9UHN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3