ID   WNT16_HUMAN             Reviewed;         365 AA.
AC   Q9UBV4; Q2M3G1; Q9Y5C0;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   24-JAN-2024, entry version 172.
DE   RecName: Full=Protein Wnt-16;
DE   Flags: Precursor;
GN   Name=WNT16;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM WNT-16B).
RX   PubMed=10500199; DOI=10.1073/pnas.96.20.11464;
RA   McWhirter J.R., Neuteboom S.T., Wancewicz E.V., Monia B.P., Downing J.R.,
RA   Murre C.;
RT   "Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT
RT   gene in pre-B acute lymphoblastoid leukemia.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:11464-11469(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM WNT-16A), AND VARIANTS ARG-82 AND
RP   ILE-263.
RC   TISSUE=Placenta;
RX   PubMed=11095990; DOI=10.1006/bbrc.2000.3852;
RA   Fear M.W., Kelsell D.P., Spurr N.K., Barnes M.R.;
RT   "Wnt-16a, a novel Wnt-16 isoform, which shows differential expression in
RT   adult human tissues.";
RL   Biochem. Biophys. Res. Commun. 278:814-820(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM WNT-16B).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   VARIANT [LARGE SCALE ANALYSIS] MET-126.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: Ligand for members of the frizzled family of seven
CC       transmembrane receptors. Probable developmental protein. May be a
CC       signaling molecule which affects the development of discrete regions of
CC       tissues. Is likely to signal over only few cell diameters (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Wnt-16b;
CC         IsoId=Q9UBV4-1; Sequence=Displayed;
CC       Name=Wnt-16a;
CC         IsoId=Q9UBV4-2; Sequence=VSP_006797;
CC   -!- TISSUE SPECIFICITY: Isoform Wnt-16b is expressed in peripheral lymphoid
CC       organs such as spleen, appendix, and lymph nodes, in kidney but not in
CC       bone marrow. Isoform Wnt-16a is expressed at significant levels only in
CC       the pancreas.
CC   -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC       receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC       inhibition. {ECO:0000250|UniProtKB:P27467,
CC       ECO:0000250|UniProtKB:P56704}.
CC   -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR   EMBL; AF169963; AAD49351.1; -; mRNA.
DR   EMBL; AF152584; AAD38052.1; -; mRNA.
DR   EMBL; AC006364; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC104919; AAI04920.1; -; mRNA.
DR   EMBL; BC104945; AAI04946.1; -; mRNA.
DR   CCDS; CCDS5781.1; -. [Q9UBV4-1]
DR   RefSeq; NP_057171.2; NM_016087.2.
DR   RefSeq; NP_476509.1; NM_057168.1. [Q9UBV4-1]
DR   AlphaFoldDB; Q9UBV4; -.
DR   SMR; Q9UBV4; -.
DR   BioGRID; 119514; 128.
DR   IntAct; Q9UBV4; 13.
DR   STRING; 9606.ENSP00000222462; -.
DR   GlyCosmos; Q9UBV4; 3 sites, No reported glycans.
DR   GlyGen; Q9UBV4; 4 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9UBV4; -.
DR   PhosphoSitePlus; Q9UBV4; -.
DR   SwissPalm; Q9UBV4; -.
DR   BioMuta; WNT16; -.
DR   DMDM; 12643875; -.
DR   jPOST; Q9UBV4; -.
DR   MassIVE; Q9UBV4; -.
DR   PaxDb; 9606-ENSP00000222462; -.
DR   PeptideAtlas; Q9UBV4; -.
DR   ProteomicsDB; 84079; -. [Q9UBV4-1]
DR   ProteomicsDB; 84080; -. [Q9UBV4-2]
DR   Antibodypedia; 17566; 252 antibodies from 31 providers.
DR   DNASU; 51384; -.
DR   Ensembl; ENST00000222462.3; ENSP00000222462.2; ENSG00000002745.13. [Q9UBV4-1]
DR   GeneID; 51384; -.
DR   KEGG; hsa:51384; -.
DR   MANE-Select; ENST00000222462.3; ENSP00000222462.2; NM_057168.2; NP_476509.1.
DR   UCSC; uc003vjw.4; human. [Q9UBV4-1]
DR   AGR; HGNC:16267; -.
DR   CTD; 51384; -.
DR   DisGeNET; 51384; -.
DR   GeneCards; WNT16; -.
DR   HGNC; HGNC:16267; WNT16.
DR   HPA; ENSG00000002745; Group enriched (cervix, skin).
DR   MIM; 606267; gene.
DR   neXtProt; NX_Q9UBV4; -.
DR   OpenTargets; ENSG00000002745; -.
DR   PharmGKB; PA38105; -.
DR   VEuPathDB; HostDB:ENSG00000002745; -.
DR   eggNOG; KOG3913; Eukaryota.
DR   GeneTree; ENSGT00940000157480; -.
DR   HOGENOM; CLU_033039_1_0_1; -.
DR   InParanoid; Q9UBV4; -.
DR   OMA; KFERWNC; -.
DR   OrthoDB; 2874082at2759; -.
DR   PhylomeDB; Q9UBV4; -.
DR   TreeFam; TF105310; -.
DR   PathwayCommons; Q9UBV4; -.
DR   Reactome; R-HSA-3238698; WNT ligand biogenesis and trafficking.
DR   Reactome; R-HSA-373080; Class B/2 (Secretin family receptors).
DR   SignaLink; Q9UBV4; -.
DR   SIGNOR; Q9UBV4; -.
DR   BioGRID-ORCS; 51384; 5 hits in 1147 CRISPR screens.
DR   GeneWiki; WNT16; -.
DR   GenomeRNAi; 51384; -.
DR   Pharos; Q9UBV4; Tbio.
DR   PRO; PR:Q9UBV4; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q9UBV4; Protein.
DR   Bgee; ENSG00000002745; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 93 other cell types or tissues.
DR   ExpressionAtlas; Q9UBV4; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IDA:BHF-UCL.
DR   GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR   GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR   GO; GO:0046849; P:bone remodeling; IEA:Ensembl.
DR   GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central.
DR   GO; GO:0060317; P:cardiac epithelial to mesenchymal transition; IEA:Ensembl.
DR   GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR   GO; GO:0030216; P:keratinocyte differentiation; IMP:BHF-UCL.
DR   GO; GO:0043616; P:keratinocyte proliferation; IMP:BHF-UCL.
DR   GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IMP:BHF-UCL.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0003408; P:optic cup formation involved in camera-type eye development; ISS:BHF-UCL.
DR   GO; GO:0090403; P:oxidative stress-induced premature senescence; IMP:BHF-UCL.
DR   GO; GO:0010628; P:positive regulation of gene expression; IMP:BHF-UCL.
DR   GO; GO:0046330; P:positive regulation of JNK cascade; IMP:BHF-UCL.
DR   GO; GO:0051897; P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; IMP:BHF-UCL.
DR   GO; GO:0090399; P:replicative senescence; IMP:BHF-UCL.
DR   GO; GO:0016055; P:Wnt signaling pathway; IMP:BHF-UCL.
DR   CDD; cd19344; Wnt_Wnt16; 1.
DR   Gene3D; 3.30.2460.20; -; 1.
DR   InterPro; IPR005817; Wnt.
DR   InterPro; IPR013304; Wnt16.
DR   InterPro; IPR043158; Wnt_C.
DR   InterPro; IPR018161; Wnt_CS.
DR   PANTHER; PTHR12027:SF70; PROTEIN WNT-16; 1.
DR   PANTHER; PTHR12027; WNT RELATED; 1.
DR   Pfam; PF00110; wnt; 1.
DR   PRINTS; PR01895; WNT16PROTEIN.
DR   PRINTS; PR01349; WNTPROTEIN.
DR   SMART; SM00097; WNT1; 1.
DR   PROSITE; PS00246; WNT1; 1.
DR   Genevisible; Q9UBV4; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Developmental protein; Disulfide bond;
KW   Extracellular matrix; Glycoprotein; Lipoprotein; Reference proteome;
KW   Secreted; Signal; Wnt signaling pathway.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..365
FT                   /note="Protein Wnt-16"
FT                   /id="PRO_0000041471"
FT   LIPID           227
FT                   /note="O-palmitoleoyl serine; by PORCN"
FT                   /evidence="ECO:0000250|UniProtKB:P56704"
FT   CARBOHYD        143
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        189
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        311
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        81..92
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        139..147
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        149..168
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        221..235
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        223..230
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        294..325
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        310..320
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        324..364
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        340..355
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        342..352
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        347..348
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   VAR_SEQ         1..32
FT                   /note="MDRAALLGLARLCALWAALLVLFPYGAQGNWM -> MERHPPMQLTTCLRET
FT                   LFTGASQKTSLW (in isoform Wnt-16a)"
FT                   /evidence="ECO:0000303|PubMed:11095990"
FT                   /id="VSP_006797"
FT   VARIANT         82
FT                   /note="G -> R (in dbSNP:rs2908004)"
FT                   /evidence="ECO:0000269|PubMed:11095990"
FT                   /id="VAR_052957"
FT   VARIANT         126
FT                   /note="V -> M (in a colorectal cancer sample; somatic
FT                   mutation)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036289"
FT   VARIANT         263
FT                   /note="T -> I (in dbSNP:rs2707466)"
FT                   /evidence="ECO:0000269|PubMed:11095990"
FT                   /id="VAR_052958"
SQ   SEQUENCE   365 AA;  40690 MW;  313A26D8FFBCC56F CRC64;
     MDRAALLGLA RLCALWAALL VLFPYGAQGN WMWLGIASFG VPEKLGCANL PLNSRQKELC
     KRKPYLLPSI REGARLGIQE CGSQFRHERW NCMITAAATT APMGASPLFG YELSSGTKET
     AFIYAVMAAG LVHSVTRSCS AGNMTECSCD TTLQNGGSAS EGWHWGGCSD DVQYGMWFSR
     KFLDFPIGNT TGKENKVLLA MNLHNNEAGR QAVAKLMSVD CRCHGVSGSC AVKTCWKTMS
     SFEKIGHLLK DKYENSIQIS DKTKRKMRRR EKDQRKIPIH KDDLLYVNKS PNYCVEDKKL
     GIPGTQGREC NRTSEGADGC NLLCCGRGYN THVVRHVERC ECKFIWCCYV RCRRCESMTD
     VHTCK
//