Q9UBV4 (WNT16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-16

Gene names

Name:

WNT16

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
Post-translational modification	Palmitoylation at Ser-227 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-81. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cardiac epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara keratinocyte differentiation Inferred from mutant phenotype PubMed 17200136. Source: BHF-UCL keratinocyte proliferation Inferred from mutant phenotype PubMed 17200136. Source: BHF-UCL negative regulation of cell death Inferred from mutant phenotype PubMed 17200136. Source: BHF-UCL optic cup formation involved in camera-type eye development Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL oxidative stress-induced premature senescence Inferred from mutant phenotype PubMed 19951988. Source: BHF-UCL positive regulation of JNK cascade Inferred from mutant phenotype PubMed 17200136. Source: BHF-UCL positive regulation of gene expression Inferred from mutant phenotype PubMed 16007226. Source: BHF-UCL positive regulation of phosphatidylinositol 3-kinase cascade Inferred from mutant phenotype PubMed 19951988. Source: BHF-UCL replicative senescence Inferred from mutant phenotype PubMed 19951988. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from direct assay PubMed 17200136. Source: BHF-UCL extracellular space Inferred from direct assay Ref.1. Source: BHF-UCL plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Wnt-16b (identifier: Q9UBV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Wnt-16a (identifier: Q9UBV4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: MDRAALLGLARLCALWAALLVLFPYGAQGNWM → MERHPPMQLTTCLRETLFTGASQKTSLW

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 365	336	Protein Wnt-16		PRO_0000041471
Amino acid modifications
	Lipidation	81	1	S-palmitoyl cysteine By similarity
	Lipidation	227	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 32	32	MDRAA…QGNWM → MERHPPMQLTTCLRETLFTG ASQKTSLW in isoform Wnt-16a.		VSP_006797
	Natural variant	82	1	G → R. Ref.2 Corresponds to variant rs2908004 [ dbSNP | Ensembl ].		VAR_052957
	Natural variant	126	1	V → M in a colorectal cancer sample; somatic mutation. Ref.5		VAR_036289
	Natural variant	263	1	T → I. Ref.2 Corresponds to variant rs2707466 [ dbSNP | Ensembl ].		VAR_052958

Sequences

	Sequence		Length	Mass (Da)
	Isoform Wnt-16b [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 313A26D8FFBCC56F	FASTA	365	40,690
	10 20 30 40 50 60 MDRAALLGLA RLCALWAALL VLFPYGAQGN WMWLGIASFG VPEKLGCANL PLNSRQKELC 70 80 90 100 110 120 KRKPYLLPSI REGARLGIQE CGSQFRHERW NCMITAAATT APMGASPLFG YELSSGTKET 130 140 150 160 170 180 AFIYAVMAAG LVHSVTRSCS AGNMTECSCD TTLQNGGSAS EGWHWGGCSD DVQYGMWFSR 190 200 210 220 230 240 KFLDFPIGNT TGKENKVLLA MNLHNNEAGR QAVAKLMSVD CRCHGVSGSC AVKTCWKTMS 250 260 270 280 290 300 SFEKIGHLLK DKYENSIQIS DKTKRKMRRR EKDQRKIPIH KDDLLYVNKS PNYCVEDKKL 310 320 330 340 350 360 GIPGTQGREC NRTSEGADGC NLLCCGRGYN THVVRHVERC ECKFIWCCYV RCRRCESMTD VHTCK
	Isoform Wnt-16a [UniParc]. Checksum: 9C823B8C92850BBF	FASTA	361	40,447
	10 20 30 40 50 60 MERHPPMQLT TCLRETLFTG ASQKTSLWWL GIASFGVPEK LGCANLPLNS RQKELCKRKP 70 80 90 100 110 120 YLLPSIREGA RLGIQECGSQ FRHERWNCMI TAAATTAPMG ASPLFGYELS SGTKETAFIY 130 140 150 160 170 180 AVMAAGLVHS VTRSCSAGNM TECSCDTTLQ NGGSASEGWH WGGCSDDVQY GMWFSRKFLD 190 200 210 220 230 240 FPIGNTTGKE NKVLLAMNLH NNEAGRQAVA KLMSVDCRCH GVSGSCAVKT CWKTMSSFEK 250 260 270 280 290 300 IGHLLKDKYE NSIQISDKTK RKMRRREKDQ RKIPIHKDDL LYVNKSPNYC VEDKKLGIPG 310 320 330 340 350 360 TQGRECNRTS EGADGCNLLC CGRGYNTHVV RHVERCECKF IWCCYVRCRR CESMTDVHTC K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia." McWhirter J.R., Neuteboom S.T., Wancewicz E.V., Monia B.P., Downing J.R., Murre C. Proc. Natl. Acad. Sci. U.S.A. 96:11464-11469(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM WNT-16B).
[2]	"Wnt-16a, a novel Wnt-16 isoform, which shows differential expression in adult human tissues." Fear M.W., Kelsell D.P., Spurr N.K., Barnes M.R. Biochem. Biophys. Res. Commun. 278:814-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM WNT-16A), VARIANTS ARG-82 AND ILE-263. Tissue: Placenta.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM WNT-16B).
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-126.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF169963 mRNA. Translation: AAD49351.1. AF152584 mRNA. Translation: AAD38052.1. AC006364 Genomic DNA. No translation available. BC104919 mRNA. Translation: AAI04920.1. BC104945 mRNA. Translation: AAI04946.1.
IPI	IPI00002791. IPI00216310.
RefSeq	NP_057171.2. NM_016087.2. NP_476509.1. NM_057168.1.
UniGene	Hs.272375.
3D structure databases
ProteinModelPortal	Q9UBV4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UBV4. 1 interaction.
STRING	9606.ENSP00000222462.
PTM databases
PhosphoSite	Q9UBV4.
Polymorphism databases
DMDM	12643875.
Proteomic databases
PaxDb	Q9UBV4.
PRIDE	Q9UBV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000222462; ENSP00000222462; ENSG00000002745.
GeneID	51384.
KEGG	hsa:51384.
UCSC	uc003vjw.3. human.
Organism-specific databases
CTD	51384.
GeneCards	GC07P120965.
HGNC	HGNC:16267. WNT16.
HPA	HPA027030.
MIM	606267. gene.
neXtProt	NX_Q9UBV4.
PharmGKB	PA38105.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238376.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q9UBV4.
KO	K01558.
OMA	CWKTMSS.
PhylomeDB	Q9UBV4.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9UBV4.
Bgee	Q9UBV4.
CleanEx	HS_WNT16.
Genevestigator	Q9UBV4.
GermOnline	ENSG00000002745. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013304. Wnt16. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF12. PTHR12027:SF12. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01895. WNT16PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51384.
NextBio	54895.
SOURCE	Search...

Entry information

Entry name

WNT16_HUMAN

Accession

Primary (citable) accession number: Q9UBV4
Secondary accession number(s): Q2M3G1, Q9Y5C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.