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Q9UBU9

- NXF1_HUMAN

UniProt

Q9UBU9 - NXF1_HUMAN

Protein

Nuclear RNA export factor 1

Gene

NXF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 164 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.3 Publications

    GO - Molecular functioni

    1. mRNA binding Source: Ensembl
    2. nucleocytoplasmic transporter activity Source: Ensembl
    3. nucleotide binding Source: InterPro
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. mRNA export from nucleus Source: UniProtKB
    3. poly(A)+ mRNA export from nucleus Source: Ensembl
    4. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, mRNA transport, Transport

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1835. Transport of Mature mRNA Derived from an Intronless Transcript.
    REACT_405. Transport of the SLBP Dependant Mature mRNA.
    REACT_424. Transport of the SLBP independent Mature mRNA.

    Protein family/group databases

    TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear RNA export factor 1
    Alternative name(s):
    Tip-associated protein
    Tip-associating protein
    mRNA export factor TAP
    Gene namesi
    Name:NXF1
    Synonyms:TAP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:8071. NXF1.

    Subcellular locationi

    Nucleusnucleoplasm. Nucleus speckle. Cytoplasm
    Note: Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles.

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nuclear inclusion body Source: Ensembl
    3. nuclear pore Source: Ensembl
    4. nuclear speck Source: UniProtKB
    5. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi71 – 711R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-78, A-81, A-82, A-89, A-91, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi78 – 781R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-81, A-82, A-89, A-91, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi81 – 811R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-82, A-89, A-91, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi82 – 821R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-89, A-91, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi89 – 891R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-91, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi91 – 911R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-89, A-97, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi97 – 971R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-89, A-91, A-98, A-100 and A-105. 1 Publication
    Mutagenesisi98 – 981R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-89, A-91, A-97, A-100 and A-105. 1 Publication
    Mutagenesisi100 – 1001R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-89, A-91, A-97, A-98 and A-105. 1 Publication
    Mutagenesisi105 – 1051R → A: Greatly reduces RNA binding and no effect on interaction with ALYREF/THOC4; when associated with A-71, A-78, A-81, A-82, A-89, A-91, A-97, A-98 and A-100. 1 Publication
    Mutagenesisi306 – 3083ERE → AAA: Decreases the export of mRNAs from the nucleus.
    Mutagenesisi383 – 3831L → R: Diminishes nuclear rim staining and 80% reduction in mRNA export activity; when associated with R-386. Complete loss of nuclear rim staining and mRNA export activity; when associated with R-386 and A-594. 1 Publication
    Mutagenesisi386 – 3861L → R: Diminishes nuclear rim staining and 80% reduction in mRNA export activity; when associated with R-383. Complete loss of nuclear rim staining and mRNA export activity; when associated with R-383 and A-594. 1 Publication
    Mutagenesisi399 – 3991D → A: 60% reduction in mRNA export activity. 1 Publication
    Mutagenesisi450 – 4534LRFR → AAAA: Abolishes interaction with THOC5 and CHTOP.
    Mutagenesisi453 – 4531R → A: Impairs intramolecular interaction between RBD and NTF2. 1 Publication
    Mutagenesisi456 – 4594KHTR → AAAA: Abolishes interaction with THOC5 and CHTOP, no effect on interaction with NXT1; enhances intramolecular interaction between RBD and NTF2, reduces RNA binding and mRNA export.
    Mutagenesisi456 – 4561K → D: Impairs intramolecular interaction between RBD and NTF2; when associated with D-459.
    Mutagenesisi459 – 4591R → D: Impairs intramolecular interaction between RBD and NTF2; when associated with D-456.
    Mutagenesisi482 – 4821D → R: 90% reduction in mRNA export activity. 1 Publication
    Mutagenesisi518 – 5181I → R: 98% reduction in mRNA export activity. 1 Publication
    Mutagenesisi521 – 5211P → Q: 35% reduction in mRNA export activity. 1 Publication
    Mutagenesisi594 – 5941W → A: Suppresses FG-nucleoporin binding. Diminishes nuclear rim staining and 88% reduction in mRNA export activity. Complete loss of nuclear rim staining and mRNA export activity; when associated with R-383 and R-386. 1 Publication
    Mutagenesisi595 – 5951D → R: Suppresses FG-nucleoporin binding.
    Mutagenesisi617 – 6171F → A: Suppresses FG-nucleoporin binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA31858.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 619618Nuclear RNA export factor 1PRO_0000220529Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei126 – 1261Nitrated tyrosineBy similarity

    Keywords - PTMi

    Acetylation, Nitration

    Proteomic databases

    MaxQBiQ9UBU9.
    PaxDbiQ9UBU9.
    PeptideAtlasiQ9UBU9.
    PRIDEiQ9UBU9.

    PTM databases

    PhosphoSiteiQ9UBU9.

    Expressioni

    Tissue specificityi

    Expressed ubiquitously.

    Gene expression databases

    ArrayExpressiQ9UBU9.
    BgeeiQ9UBU9.
    CleanExiHS_NXF1.
    GenevestigatoriQ9UBU9.

    Organism-specific databases

    HPAiCAB016327.

    Interactioni

    Subunit structurei

    Heterodimer with NXT1. Interacts (via NTF2 domain) with NXT1. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Interacts with NXT2, E1B-AP5, RAE1, ALYREF/THOC4, DDX39B, FYTTD1/UIF, EIF4A3, NUPL2 and with several nucleoporins. Interacts with THOC5 and CHTOP; in a mutually exclusive manner. Associates with the exon junction complex (EJC) and with the transcription/export (TREX) complex. Found in a mRNA complex with RENT3A and RENT3B. Interacts with Saimiriine herpesvirus 2 TIP protein. Interacts with human herpes virus 1 (HHV-1) ICP27 protein; this interaction allows efficient export of HHV-1 early and late transcripts. Interacts with FRG1 (via N-terminus).14 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P225756EBI-398874,EBI-866709From a different organism.
    ALYREFQ86V814EBI-398874,EBI-347640
    CHTOPQ9Y3Y29EBI-398874,EBI-347794
    DDX3XO005715EBI-398874,EBI-353779
    DHX9Q082118EBI-398874,EBI-352022
    NUP62P371983EBI-398874,EBI-347978
    NXT1Q9UKK64EBI-398874,EBI-301889
    SRSF1Q079555EBI-398874,EBI-398920
    SRSF3P841034EBI-398874,EBI-372557
    SRSF7Q166294EBI-398874,EBI-398885
    THRAP3Q9Y2W14EBI-398874,EBI-352039
    UL54P102384EBI-398874,EBI-6883946From a different organism.

    Protein-protein interaction databases

    BioGridi115745. 94 interactions.
    DIPiDIP-31789N.
    IntActiQ9UBU9. 59 interactions.
    MINTiMINT-121360.
    STRINGi9606.ENSP00000294172.

    Structurei

    Secondary structure

    1
    619
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi120 – 1245
    Helixi127 – 1293
    Helixi132 – 14110
    Beta strandi143 – 1453
    Beta strandi150 – 1556
    Beta strandi158 – 1647
    Helixi166 – 1749
    Turni176 – 1783
    Beta strandi190 – 1934
    Helixi198 – 2014
    Helixi206 – 21813
    Turni222 – 2254
    Beta strandi226 – 2283
    Helixi232 – 2343
    Helixi236 – 2405
    Helixi250 – 26314
    Beta strandi269 – 2713
    Helixi281 – 2855
    Helixi286 – 2894
    Beta strandi295 – 2973
    Helixi306 – 3127
    Beta strandi318 – 3214
    Helixi328 – 3303
    Helixi334 – 34411
    Beta strandi350 – 3534
    Helixi381 – 39818
    Helixi403 – 4086
    Beta strandi410 – 41910
    Helixi433 – 4364
    Turni442 – 4443
    Helixi448 – 4547
    Beta strandi455 – 4584
    Helixi459 – 4668
    Beta strandi472 – 4743
    Helixi476 – 4783
    Beta strandi480 – 4867
    Beta strandi491 – 50111
    Turni505 – 5084
    Beta strandi510 – 52112
    Beta strandi525 – 53814
    Helixi541 – 5488
    Beta strandi553 – 5575
    Helixi565 – 57814
    Helixi582 – 59110
    Turni592 – 5943
    Helixi596 – 60813
    Helixi614 – 6174

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FO1X-ray2.90A/B102-372[»]
    1FT8X-ray3.15A/B/C/D/E102-372[»]
    1GO5NMR-A551-619[»]
    1JKGX-ray1.90B371-619[»]
    1JN5X-ray2.80B371-619[»]
    1KOHX-ray3.80A/B/C/D96-372[»]
    1KOOX-ray3.80A/B/C/D96-372[»]
    1OAIX-ray1.00A561-619[»]
    2Z5KX-ray2.60B53-82[»]
    2Z5MX-ray3.00B53-82[»]
    3RW6X-ray2.30A/B96-362[»]
    3RW7X-ray3.00A/B/C/D96-362[»]
    ProteinModelPortaliQ9UBU9.
    SMRiQ9UBU9. Positions 118-362, 371-619.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UBU9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini119 – 19880RRMAdd
    BLAST
    Repeati266 – 29126LRR 1Add
    BLAST
    Repeati292 – 31524LRR 2Add
    BLAST
    Repeati316 – 34328LRR 3Add
    BLAST
    Repeati344 – 37128LRR 4Add
    BLAST
    Domaini386 – 536151NTF2PROSITE-ProRule annotationAdd
    BLAST
    Domaini565 – 61955TAP-CPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 198197Interaction with ALYREF/THOC4Add
    BLAST
    Regioni2 – 118117RNA-binding (RBD)Add
    BLAST
    Regioni2 – 6059Minor non-specific RNA-bindingAdd
    BLAST
    Regioni61 – 11858Major non-specific RNA-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi67 – 10034Nuclear localization signalAdd
    BLAST
    Motifi83 – 11028Nuclear export signalAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi551 – 56111Pro-richAdd
    BLAST

    Domaini

    The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.1 Publication
    The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.1 Publication
    The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus. It inhibits RNA binding activity through an intramolecular interaction with the N-terminal RNA binding domain (RBD); the inhibition is removed by an association with the TREX complex, specifically involving ALYREF/THOC4 and THOC5.1 Publication
    The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.1 Publication
    The leucine-rich repeats are essential for the export of mRNA from the nucleus.1 Publication
    The RNA-binding domain is a non-canonical RNP-type domain.1 Publication

    Sequence similaritiesi

    Belongs to the NXF family.Curated
    Contains 4 LRR (leucine-rich) repeats.Curated
    Contains 1 NTF2 domain.PROSITE-ProRule annotation
    Contains 1 TAP-C domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG324438.
    HOGENOMiHOG000236269.
    HOVERGENiHBG013199.
    InParanoidiQ9UBU9.
    KOiK14284.
    OMAiYAVYDSG.
    OrthoDBiEOG7QZGB9.
    PhylomeDBiQ9UBU9.
    TreeFamiTF314566.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR001611. Leu-rich_rpt.
    IPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR005637. TAP_C_dom.
    IPR015245. Tap_RNA-bd.
    IPR003603. U2A'_phosphoprotein32A_C.
    IPR009060. UBA-like.
    [Graphical view]
    PfamiPF00560. LRR_1. 1 hit.
    PF02136. NTF2. 1 hit.
    PF09162. Tap-RNA_bind. 1 hit.
    PF03943. TAP_C. 1 hit.
    [Graphical view]
    ProDomiPD043466. Tap_RNA_bd. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00446. LRRcap. 1 hit.
    SM00804. TAP_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS51450. LRR. 3 hits.
    PS50177. NTF2_DOMAIN. 1 hit.
    PS51281. TAP_C. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UBU9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADEGKSYSE HDDERVNFPQ RKKKGRGPFR WKYGEGNRRS GRGGSGIRSS    50
    RLEEDDGDVA MSDAQDGPRV RYNPYTTRPN RRGDTWHDRD RIHVTVRRDR 100
    APPERGGAGT SQDGTSKNWF KITIPYGRKY DKAWLLSMIQ SKCSVPFTPI 150
    EFHYENTRAQ FFVEDASTAS ALKAVNYKIL DRENRRISII INSSAPPHTI 200
    LNELKPEQVE QLKLIMSKRY DGSQQALDLK GLRSDPDLVA QNIDVVLNRR 250
    SCMAATLRII EENIPELLSL NLSNNRLYRL DDMSSIVQKA PNLKILNLSG 300
    NELKSERELD KIKGLKLEEL WLDGNSLCDT FRDQSTYISA IRERFPKLLR 350
    LDGHELPPPI AFDVEAPTTL PPCKGSYFGT ENLKSLVLHF LQQYYAIYDS 400
    GDRQGLLDAY HDGACCSLSI PFIPQNPARS SLAEYFKDSR NVKKLKDPTL 450
    RFRLLKHTRL NVVAFLNELP KTQHDVNSFV VDISAQTSTL LCFSVNGVFK 500
    EVDGKSRDSL RAFTRTFIAV PASNSGLCIV NDELFVRNAS SEEIQRAFAM 550
    PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA 600
    QAFTHLKAKG EIPEVAFMK 619
    Length:619
    Mass (Da):70,182
    Last modified:May 1, 2000 - v1
    Checksum:i338872AADA789FBF
    GO
    Isoform 2 (identifier: Q9UBU9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         339-356: SAIRERFPKLLRLDGHEL → RSVVACVSPPGDLHPLGG
         357-619: Missing.

    Show »
    Length:356
    Mass (Da):40,476
    Checksum:i470701F5324EC0B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti119 – 1191W → C in AAB81111. (PubMed:9175835)Curated
    Sequence conflicti256 – 2561T → N in AAD20016. (PubMed:10454577)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei339 – 35618SAIRE…DGHEL → RSVVACVSPPGDLHPLGG in isoform 2. 1 PublicationVSP_041427Add
    BLAST
    Alternative sequencei357 – 619263Missing in isoform 2. 1 PublicationVSP_041428Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132712 mRNA. Translation: CAA10753.1.
    AF112880 mRNA. Translation: AAD39102.1.
    AF126246 mRNA. Translation: AAD20016.1.
    AK304137 mRNA. Translation: BAG65031.1.
    AK027192 mRNA. No translation available.
    AP001160 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW74105.1.
    BC004904 mRNA. Translation: AAH04904.1.
    BC028041 mRNA. Translation: AAH28041.1.
    U80073 mRNA. Translation: AAB81111.1.
    CCDSiCCDS44629.1. [Q9UBU9-2]
    CCDS8037.1. [Q9UBU9-1]
    RefSeqiNP_001074960.1. NM_001081491.1. [Q9UBU9-2]
    NP_006353.2. NM_006362.4. [Q9UBU9-1]
    UniGeneiHs.523739.
    Hs.601546.

    Genome annotation databases

    EnsembliENST00000294172; ENSP00000294172; ENSG00000162231. [Q9UBU9-1]
    ENST00000531709; ENSP00000453885; ENSG00000162231. [Q9UBU9-2]
    ENST00000532297; ENSP00000436679; ENSG00000162231. [Q9UBU9-1]
    GeneIDi10482.
    KEGGihsa:10482.
    UCSCiuc001nvf.1. human. [Q9UBU9-1]
    uc001nvg.1. human. [Q9UBU9-2]

    Polymorphism databases

    DMDMi20139282.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132712 mRNA. Translation: CAA10753.1 .
    AF112880 mRNA. Translation: AAD39102.1 .
    AF126246 mRNA. Translation: AAD20016.1 .
    AK304137 mRNA. Translation: BAG65031.1 .
    AK027192 mRNA. No translation available.
    AP001160 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW74105.1 .
    BC004904 mRNA. Translation: AAH04904.1 .
    BC028041 mRNA. Translation: AAH28041.1 .
    U80073 mRNA. Translation: AAB81111.1 .
    CCDSi CCDS44629.1. [Q9UBU9-2 ]
    CCDS8037.1. [Q9UBU9-1 ]
    RefSeqi NP_001074960.1. NM_001081491.1. [Q9UBU9-2 ]
    NP_006353.2. NM_006362.4. [Q9UBU9-1 ]
    UniGenei Hs.523739.
    Hs.601546.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FO1 X-ray 2.90 A/B 102-372 [» ]
    1FT8 X-ray 3.15 A/B/C/D/E 102-372 [» ]
    1GO5 NMR - A 551-619 [» ]
    1JKG X-ray 1.90 B 371-619 [» ]
    1JN5 X-ray 2.80 B 371-619 [» ]
    1KOH X-ray 3.80 A/B/C/D 96-372 [» ]
    1KOO X-ray 3.80 A/B/C/D 96-372 [» ]
    1OAI X-ray 1.00 A 561-619 [» ]
    2Z5K X-ray 2.60 B 53-82 [» ]
    2Z5M X-ray 3.00 B 53-82 [» ]
    3RW6 X-ray 2.30 A/B 96-362 [» ]
    3RW7 X-ray 3.00 A/B/C/D 96-362 [» ]
    ProteinModelPortali Q9UBU9.
    SMRi Q9UBU9. Positions 118-362, 371-619.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115745. 94 interactions.
    DIPi DIP-31789N.
    IntActi Q9UBU9. 59 interactions.
    MINTi MINT-121360.
    STRINGi 9606.ENSP00000294172.

    Protein family/group databases

    TCDBi 3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

    PTM databases

    PhosphoSitei Q9UBU9.

    Polymorphism databases

    DMDMi 20139282.

    Proteomic databases

    MaxQBi Q9UBU9.
    PaxDbi Q9UBU9.
    PeptideAtlasi Q9UBU9.
    PRIDEi Q9UBU9.

    Protocols and materials databases

    DNASUi 10482.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000294172 ; ENSP00000294172 ; ENSG00000162231 . [Q9UBU9-1 ]
    ENST00000531709 ; ENSP00000453885 ; ENSG00000162231 . [Q9UBU9-2 ]
    ENST00000532297 ; ENSP00000436679 ; ENSG00000162231 . [Q9UBU9-1 ]
    GeneIDi 10482.
    KEGGi hsa:10482.
    UCSCi uc001nvf.1. human. [Q9UBU9-1 ]
    uc001nvg.1. human. [Q9UBU9-2 ]

    Organism-specific databases

    CTDi 10482.
    GeneCardsi GC11M062559.
    HGNCi HGNC:8071. NXF1.
    HPAi CAB016327.
    MIMi 602647. gene.
    neXtProti NX_Q9UBU9.
    PharmGKBi PA31858.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG324438.
    HOGENOMi HOG000236269.
    HOVERGENi HBG013199.
    InParanoidi Q9UBU9.
    KOi K14284.
    OMAi YAVYDSG.
    OrthoDBi EOG7QZGB9.
    PhylomeDBi Q9UBU9.
    TreeFami TF314566.

    Enzyme and pathway databases

    Reactomei REACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1835. Transport of Mature mRNA Derived from an Intronless Transcript.
    REACT_405. Transport of the SLBP Dependant Mature mRNA.
    REACT_424. Transport of the SLBP independent Mature mRNA.

    Miscellaneous databases

    ChiTaRSi NXF1. human.
    EvolutionaryTracei Q9UBU9.
    GeneWikii NXF1.
    GenomeRNAii 10482.
    NextBioi 39766.
    PROi Q9UBU9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UBU9.
    Bgeei Q9UBU9.
    CleanExi HS_NXF1.
    Genevestigatori Q9UBU9.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR001611. Leu-rich_rpt.
    IPR002075. NTF2.
    IPR018222. Nuclear_transport_factor_2_euk.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR005637. TAP_C_dom.
    IPR015245. Tap_RNA-bd.
    IPR003603. U2A'_phosphoprotein32A_C.
    IPR009060. UBA-like.
    [Graphical view ]
    Pfami PF00560. LRR_1. 1 hit.
    PF02136. NTF2. 1 hit.
    PF09162. Tap-RNA_bind. 1 hit.
    PF03943. TAP_C. 1 hit.
    [Graphical view ]
    ProDomi PD043466. Tap_RNA_bd. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00446. LRRcap. 1 hit.
    SM00804. TAP_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS51450. LRR. 3 hits.
    PS50177. NTF2_DOMAIN. 1 hit.
    PS51281. TAP_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus."
      Braun I.C., Rohrbach E., Schmitt C., Izaurralde E.
      EMBO J. 18:1953-1965(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS.
      Tissue: Cervix carcinoma.
    2. "The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences."
      Kang Y., Cullen B.R.
      Genes Dev. 13:1126-1139(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs."
      Bear J., Tan W., Zolotukhin A.S., Tabernero C., Hudson E.A., Felber B.K.
      Mol. Cell. Biol. 19:6306-6317(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung and Trachea.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain and Placenta.
    8. "Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation."
      Yoon D.-W., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung J.U.
      Immunity 6:571-582(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-619, INTERACTION WITH SAIMIRIINE HERPESVIRUS 2 TIP.
      Tissue: Lymphocyte.
    9. "TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus."
      Grueter P., Tabernero C., von Kobbe C., Schmitt C., Saavedra C., Bachi A., Wilm M., Felber B.K., Izaurralde E.
      Mol. Cell 1:649-659(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human."
      Katahira J., Straesser K., Podtelejnikov A., Mann M., Jung J.U., Hurt E.
      EMBO J. 18:2593-2609(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NUPL2.
    11. "Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export."
      Braun I.C., Herold A., Rode M., Conti E., Izaurralde E.
      J. Biol. Chem. 276:20536-20543(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    12. "The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates."
      Bachi A., Braun I.C., Rodrigues J.P., Pante N., Ribbeck K., von Kobbe C., Kutay U., Wilm M., Goerlich D., Carmo-Fonseca M., Izaurralde E.
      RNA 6:136-158(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    13. "Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins."
      Suyama M., Doerks T., Braun I.C., Sattler M., Izaurralde E., Bork P.
      EMBO Rep. 1:53-58(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    14. "Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex."
      Kataoka N., Diem M.D., Kim V.N., Yong J., Dreyfuss G.
      EMBO J. 20:6424-6433(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALYREF/THOC4 AND THE EXON JUNCTION COMPLEX.
    15. "Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex."
      Kim V.N., Kataoka N., Dreyfuss G.
      Science 293:1832-1836(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A MRNP COMPLEX WITH UPF3A AND UPF3B.
    16. "Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1."
      Lykke-Andersen J., Shu M.-D., Steitz J.A.
      Science 293:1836-1839(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A POST-SPLICING COMPLEX WITH RBM8A; UPF1; UPF2; UPF3A; UPF3B AND RNPS1.
    17. "eIF4A3 is a novel component of the exon junction complex."
      Chan C.C., Dostie J., Diem M.D., Feng W., Mann M., Rappsilber J., Dreyfuss G.
      RNA 10:200-209(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EIF4A3 AND ALYREF/THOC4.
    18. "Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP."
      Hautbergue G.M., Hung M.L., Golovanov A.P., Lian L.Y., Wilson S.A.
      Proc. Natl. Acad. Sci. U.S.A. 105:5154-5159(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ARG-71; ARG-78; ARG-81; ARG-82; ARG-89; ARG-91; ARG-97; ARG-98; ARG-100 AND ARG-105.
    19. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    20. "UIF, a new mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA."
      Hautbergue G.M., Hung M.L., Walsh M.J., Snijders A.P., Chang C.T., Jones R., Ponting C.P., Dickman M.J., Wilson S.A.
      Curr. Biol. 19:1918-1924(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FYTTD1.
    21. "Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA."
      Katahira J., Inoue H., Hurt E., Yoneda Y.
      EMBO J. 28:556-567(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ALYREF/THOC4 AND THOC5.
    22. "Assembly and mobility of exon-exon junction complexes in living cells."
      Schmidt U., Im K.-B., Benzing C., Janjetovic S., Rippe K., Lichter P., Wachsmuth M.
      RNA 15:862-876(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    23. "The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable."
      Johnson L.A., Li L., Sandri-Goldin R.M.
      J. Virol. 83:6335-6346(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN HERPES VIRUS 1 ICP27.
    24. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    25. "Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein."
      Sun C.Y., van Koningsbruggen S., Long S.W., Straasheijm K., Klooster R., Jones T.I., Bellini M., Levesque L., Brieher W.M., van der Maarel S.M., Jones P.L.
      J. Mol. Biol. 411:397-416(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FRG1.
    26. Cited for: ASSOCIATION WITH THE TREX COMPLEX, RNA-BINDING, MUTAGENESIS OF ARG-453 AND 456-LYS--ARG-459.
    27. Cited for: INTERACTION WITH CHTOP; THOC5 AND ALYREF, MUTAGENESIS OF 450-LEU--ARG-453 AND 456-LYS--ARG-459.
    28. "Mapping interactions between mRNA export factors in living cells."
      Teng I.F., Wilson S.A.
      PLoS ONE 8:E67676-E67676(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CHTOP.
    29. "The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain."
      Liker E., Fernandez E., Izaurralde E., Conti E.
      EMBO J. 19:5587-5598(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 102-372.
    30. "Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor."
      Fribourg S., Braun I.C., Izaurralde E., Conti E.
      Mol. Cell 8:645-656(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH NXT1, X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NXT1-FG-REPEAT, SUBUNIT, DOMAINS, MUTAGENESIS OF LEU-383; LEU-386; ASP-399; ASP-482; ILE-518; PRO-521 AND TRP-594.
    31. "Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution."
      Grant R.P., Neuhaus D., Stewart M.
      J. Mol. Biol. 326:849-858(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS) OF 561-619 IN COMPLEX WITH RANBP3.
    32. "Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1."
      Grant R.P., Hurt E., Neuhaus D., Stewart M.
      Nat. Struct. Biol. 9:247-251(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 551-619, MUTAGENESIS OF PHE-617.

    Entry informationi

    Entry nameiNXF1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UBU9
    Secondary accession number(s): B4E269, Q99799, Q9UQL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 164 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3