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Protein

Protein DBF4 homolog A

Gene

DBF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri289 – 337DBF4-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

  • enzyme activator activity Source: ProtInc
  • nucleic acid binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000006634-MONOMER.
ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-68962. Activation of the pre-replicative complex.
SIGNORiQ9UBU7.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog A
Alternative name(s):
Activator of S phase kinase
Chiffon homolog A
DBF4-type zinc finger-containing protein 1
Gene namesi
Name:DBF4
Synonyms:ASK, DBF4A, ZDBF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:17364. DBF4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10926.
OpenTargetsiENSG00000006634.
PharmGKBiPA142672016.

Chemistry databases

ChEMBLiCHEMBL4483.

Polymorphism and mutation databases

BioMutaiDBF4.
DMDMi74753231.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340611 – 674Protein DBF4 homolog AAdd BLAST674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei345PhosphothreonineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei359PhosphoserineCombined sources1
Modified residuei381PhosphoserineCombined sources1
Modified residuei413PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1
Modified residuei553PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UBU7.
PaxDbiQ9UBU7.
PeptideAtlasiQ9UBU7.
PRIDEiQ9UBU7.

PTM databases

iPTMnetiQ9UBU7.
PhosphoSitePlusiQ9UBU7.

Expressioni

Tissue specificityi

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.1 Publication

Inductioni

Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.2 Publications

Gene expression databases

BgeeiENSG00000006634.
CleanExiHS_DBF4.
ExpressionAtlasiQ9UBU7. baseline and differential.
GenevisibleiQ9UBU7. HS.

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6.4 Publications

Protein-protein interaction databases

BioGridi116129. 22 interactors.
DIPiDIP-31205N.
IntActiQ9UBU7. 4 interactors.
MINTiMINT-2842143.
STRINGi9606.ENSP00000265728.

Chemistry databases

BindingDBiQ9UBU7.

Structurei

Secondary structure

1674
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi217 – 225Combined sources9
Beta strandi227 – 229Combined sources3
Beta strandi232 – 235Combined sources4
Beta strandi244 – 246Combined sources3
Turni297 – 300Combined sources4
Helixi306 – 311Combined sources6
Helixi313 – 319Combined sources7
Helixi322 – 324Combined sources3
Helixi325 – 331Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F99X-ray2.33B210-350[»]
4F9AX-ray2.17B/D210-350[»]
4F9BX-ray2.50B/D210-350[»]
4F9CX-ray2.08B210-350[»]
ProteinModelPortaliQ9UBU7.
SMRiQ9UBU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 128BRCT 1Add BLAST89
Domaini154 – 179BRCT 2Add BLAST26

Sequence similaritiesi

Contains 2 BRCT domains.Curated
Contains 1 DBF4-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri289 – 337DBF4-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOVERGENiHBG063274.
InParanoidiQ9UBU7.
KOiK06629.
OMAiDIWEEEN.
OrthoDBiEOG091G06P9.
PhylomeDBiQ9UBU7.
TreeFamiTF332790.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV
60 70 80 90 100
FYLDLPSVTI SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG
110 120 130 140 150
RISPVPSPES AYTAETTSPH PSHDGSSFKS PDTVCLSRGK LLVEKAIKDH
160 170 180 190 200
DFIPSNSILS NALSWGVKIL HIDDIRYYIE QKKKELYLLK KSSTSVRDGG
210 220 230 240 250
KRVGSGAQKT RTGRLKKPFV KVEDMSQLYR PFYLQLTNMP FINYSIQKPC
260 270 280 290 300
SPFDVDKPSS MQKQTQVKLR IQTDGDKYGG TSIQLQLKEK KKKGYCECCL
310 320 330 340 350
QKYEDLETHL LSEQHRNFAQ SNQYQVVDDI VSKLVFDFVE YEKDTPKKKR
360 370 380 390 400
IKYSVGSLSP VSASVLKKTE QKEKVELQHI SQKDCQEDDT TVKEQNFLYK
410 420 430 440 450
ETQETEKKLL FISEPIPHPS NELRGLNEKM SNKCSMLSTA EDDIRQNFTQ
460 470 480 490 500
LPLHKNKQEC ILDISEHTLS ENDLEELRVD HYKCNIQASV HVSDFSTDNS
510 520 530 540 550
GSQPKQKSDT VLFPAKDLKE KDLHSIFTHD SGLITINSSQ EHLTVQAKAP
560 570 580 590 600
FHTPPEEPNE CDFKNMDSLP SGKIHRKVKI ILGRNRKENL EPNAEFDKRT
610 620 630 640 650
EFITQEENRI CSSPVQSLLD LFQTSEEKSE FLGFTSYTEK SGICNVLDIW
660 670
EEENSDNLLT AFFSSPSTST FTGF
Length:674
Mass (Da):76,858
Last modified:May 1, 2000 - v1
Checksum:i353FEB7E85507E5C
GO
Isoform 2 (identifier: Q9UBU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-234: LYRPFYL → SPAVHLM
     235-674: Missing.

Show »
Length:234
Mass (Da):26,124
Checksum:i50357B9FCF8472BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti581I → L in EAL24170 (PubMed:12690205).Curated1
Sequence conflicti584R → Q in EAL24170 (PubMed:12690205).Curated1
Sequence conflicti619L → P in EAL24170 (PubMed:12690205).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052970112Y → N.Corresponds to variant rs1476703dbSNPEnsembl.1
Natural variantiVAR_052971575H → R.Corresponds to variant rs2041049dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018203228 – 234LYRPFYL → SPAVHLM in isoform 2. 1 Publication7
Alternative sequenceiVSP_018204235 – 674Missing in isoform 2. 1 PublicationAdd BLAST440

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028069 mRNA. Translation: BAA78326.1.
AB028070 mRNA. Translation: BAA78327.1.
AF160249 mRNA. Translation: AAD41911.1.
AF160876 mRNA. Translation: AAD45357.1.
AK292569 mRNA. Translation: BAF85258.1.
AC003083 Genomic DNA. Translation: AAS07442.1.
AC005164 Genomic DNA. Translation: AAS07418.1.
CH236949 Genomic DNA. Translation: EAL24170.1.
CH471091 Genomic DNA. Translation: EAW76930.1.
BC036045 mRNA. Translation: AAH36045.1.
BC047693 mRNA. Translation: AAH47693.1.
CCDSiCCDS5611.1. [Q9UBU7-1]
PIRiT02633.
RefSeqiNP_001304989.1. NM_001318060.1.
NP_001304990.1. NM_001318061.1.
NP_001304991.1. NM_001318062.1.
NP_006707.1. NM_006716.3. [Q9UBU7-1]
UniGeneiHs.485380.

Genome annotation databases

EnsembliENST00000265728; ENSP00000265728; ENSG00000006634. [Q9UBU7-1]
ENST00000413643; ENSP00000414083; ENSG00000006634. [Q9UBU7-2]
GeneIDi10926.
KEGGihsa:10926.
UCSCiuc003ujf.1. human. [Q9UBU7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028069 mRNA. Translation: BAA78326.1.
AB028070 mRNA. Translation: BAA78327.1.
AF160249 mRNA. Translation: AAD41911.1.
AF160876 mRNA. Translation: AAD45357.1.
AK292569 mRNA. Translation: BAF85258.1.
AC003083 Genomic DNA. Translation: AAS07442.1.
AC005164 Genomic DNA. Translation: AAS07418.1.
CH236949 Genomic DNA. Translation: EAL24170.1.
CH471091 Genomic DNA. Translation: EAW76930.1.
BC036045 mRNA. Translation: AAH36045.1.
BC047693 mRNA. Translation: AAH47693.1.
CCDSiCCDS5611.1. [Q9UBU7-1]
PIRiT02633.
RefSeqiNP_001304989.1. NM_001318060.1.
NP_001304990.1. NM_001318061.1.
NP_001304991.1. NM_001318062.1.
NP_006707.1. NM_006716.3. [Q9UBU7-1]
UniGeneiHs.485380.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F99X-ray2.33B210-350[»]
4F9AX-ray2.17B/D210-350[»]
4F9BX-ray2.50B/D210-350[»]
4F9CX-ray2.08B210-350[»]
ProteinModelPortaliQ9UBU7.
SMRiQ9UBU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116129. 22 interactors.
DIPiDIP-31205N.
IntActiQ9UBU7. 4 interactors.
MINTiMINT-2842143.
STRINGi9606.ENSP00000265728.

Chemistry databases

BindingDBiQ9UBU7.
ChEMBLiCHEMBL4483.

PTM databases

iPTMnetiQ9UBU7.
PhosphoSitePlusiQ9UBU7.

Polymorphism and mutation databases

BioMutaiDBF4.
DMDMi74753231.

Proteomic databases

EPDiQ9UBU7.
PaxDbiQ9UBU7.
PeptideAtlasiQ9UBU7.
PRIDEiQ9UBU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265728; ENSP00000265728; ENSG00000006634. [Q9UBU7-1]
ENST00000413643; ENSP00000414083; ENSG00000006634. [Q9UBU7-2]
GeneIDi10926.
KEGGihsa:10926.
UCSCiuc003ujf.1. human. [Q9UBU7-1]

Organism-specific databases

CTDi10926.
DisGeNETi10926.
GeneCardsiDBF4.
HGNCiHGNC:17364. DBF4.
MIMi604281. gene.
neXtProtiNX_Q9UBU7.
OpenTargetsiENSG00000006634.
PharmGKBiPA142672016.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4139. Eukaryota.
COG5067. LUCA.
GeneTreeiENSGT00530000063909.
HOVERGENiHBG063274.
InParanoidiQ9UBU7.
KOiK06629.
OMAiDIWEEEN.
OrthoDBiEOG091G06P9.
PhylomeDBiQ9UBU7.
TreeFamiTF332790.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000006634-MONOMER.
ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-68962. Activation of the pre-replicative complex.
SIGNORiQ9UBU7.

Miscellaneous databases

GeneWikiiDBF4.
GenomeRNAii10926.
PROiQ9UBU7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000006634.
CleanExiHS_DBF4.
ExpressionAtlasiQ9UBU7. baseline and differential.
GenevisibleiQ9UBU7. HS.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR006572. Znf_DBF.
[Graphical view]
PfamiPF07535. zf-DBF. 1 hit.
[Graphical view]
SMARTiSM00586. ZnF_DBF. 1 hit.
[Graphical view]
PROSITEiPS51265. ZF_DBF4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBF4A_HUMAN
AccessioniPrimary (citable) accession number: Q9UBU7
Secondary accession number(s): A4D1D8
, A8K954, O75226, Q75MS6, Q75N01, Q9Y2M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.