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Protein

Protein DBF4 homolog A

Gene

DBF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri289 – 337DBF4-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

  • enzyme activator activity Source: ProtInc
  • nucleic acid binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-176187 Activation of ATR in response to replication stress
R-HSA-68962 Activation of the pre-replicative complex
SIGNORiQ9UBU7

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DBF4 homolog A
Alternative name(s):
Activator of S phase kinase
Chiffon homolog A
DBF4-type zinc finger-containing protein 1
Gene namesi
Name:DBF4
Synonyms:ASK, DBF4A, ZDBF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000006634.7
HGNCiHGNC:17364 DBF4
MIMi604281 gene
neXtProtiNX_Q9UBU7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10926
OpenTargetsiENSG00000006634
PharmGKBiPA142672016

Chemistry databases

ChEMBLiCHEMBL4483

Polymorphism and mutation databases

BioMutaiDBF4
DMDMi74753231

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002340611 – 674Protein DBF4 homolog AAdd BLAST674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei345PhosphothreonineCombined sources1
Modified residuei354PhosphoserineCombined sources1
Modified residuei359PhosphoserineCombined sources1
Modified residuei381PhosphoserineCombined sources1
Modified residuei413PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1
Modified residuei553PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UBU7
PaxDbiQ9UBU7
PeptideAtlasiQ9UBU7
PRIDEiQ9UBU7

PTM databases

iPTMnetiQ9UBU7
PhosphoSitePlusiQ9UBU7

Expressioni

Tissue specificityi

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.1 Publication

Inductioni

Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.2 Publications

Gene expression databases

BgeeiENSG00000006634
CleanExiHS_DBF4
ExpressionAtlasiQ9UBU7 baseline and differential
GenevisibleiQ9UBU7 HS

Organism-specific databases

HPAiHPA042923

Interactioni

Subunit structurei

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC7O003113EBI-16017435,EBI-374980

Protein-protein interaction databases

BioGridi116129, 23 interactors
CORUMiQ9UBU7
DIPiDIP-31205N
IntActiQ9UBU7, 4 interactors
MINTiQ9UBU7
STRINGi9606.ENSP00000265728

Chemistry databases

BindingDBiQ9UBU7

Structurei

Secondary structure

1674
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi217 – 225Combined sources9
Beta strandi227 – 229Combined sources3
Beta strandi232 – 235Combined sources4
Beta strandi244 – 246Combined sources3
Turni297 – 300Combined sources4
Helixi306 – 311Combined sources6
Helixi313 – 319Combined sources7
Helixi322 – 324Combined sources3
Helixi325 – 331Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F99X-ray2.33B210-350[»]
4F9AX-ray2.17B/D210-350[»]
4F9BX-ray2.50B/D210-350[»]
4F9CX-ray2.08B210-350[»]
ProteinModelPortaliQ9UBU7
SMRiQ9UBU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 128BRCT 1Add BLAST89
Domaini154 – 179BRCT 2Add BLAST26

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri289 – 337DBF4-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4139 Eukaryota
COG5067 LUCA
GeneTreeiENSGT00530000063909
HOVERGENiHBG063274
InParanoidiQ9UBU7
KOiK06629
OMAiDIWEEEN
OrthoDBiEOG091G06P9
PhylomeDBiQ9UBU7
TreeFamiTF332790

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.30.160.680, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR006572 Znf_DBF
IPR038545 Znf_DBF_sf
PfamiView protein in Pfam
PF07535 zf-DBF, 1 hit
SMARTiView protein in SMART
SM00586 ZnF_DBF, 1 hit
PROSITEiView protein in PROSITE
PS51265 ZF_DBF4, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV
60 70 80 90 100
FYLDLPSVTI SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG
110 120 130 140 150
RISPVPSPES AYTAETTSPH PSHDGSSFKS PDTVCLSRGK LLVEKAIKDH
160 170 180 190 200
DFIPSNSILS NALSWGVKIL HIDDIRYYIE QKKKELYLLK KSSTSVRDGG
210 220 230 240 250
KRVGSGAQKT RTGRLKKPFV KVEDMSQLYR PFYLQLTNMP FINYSIQKPC
260 270 280 290 300
SPFDVDKPSS MQKQTQVKLR IQTDGDKYGG TSIQLQLKEK KKKGYCECCL
310 320 330 340 350
QKYEDLETHL LSEQHRNFAQ SNQYQVVDDI VSKLVFDFVE YEKDTPKKKR
360 370 380 390 400
IKYSVGSLSP VSASVLKKTE QKEKVELQHI SQKDCQEDDT TVKEQNFLYK
410 420 430 440 450
ETQETEKKLL FISEPIPHPS NELRGLNEKM SNKCSMLSTA EDDIRQNFTQ
460 470 480 490 500
LPLHKNKQEC ILDISEHTLS ENDLEELRVD HYKCNIQASV HVSDFSTDNS
510 520 530 540 550
GSQPKQKSDT VLFPAKDLKE KDLHSIFTHD SGLITINSSQ EHLTVQAKAP
560 570 580 590 600
FHTPPEEPNE CDFKNMDSLP SGKIHRKVKI ILGRNRKENL EPNAEFDKRT
610 620 630 640 650
EFITQEENRI CSSPVQSLLD LFQTSEEKSE FLGFTSYTEK SGICNVLDIW
660 670
EEENSDNLLT AFFSSPSTST FTGF
Length:674
Mass (Da):76,858
Last modified:May 1, 2000 - v1
Checksum:i353FEB7E85507E5C
GO
Isoform 2 (identifier: Q9UBU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-234: LYRPFYL → SPAVHLM
     235-674: Missing.

Show »
Length:234
Mass (Da):26,124
Checksum:i50357B9FCF8472BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti581I → L in EAL24170 (PubMed:12690205).Curated1
Sequence conflicti584R → Q in EAL24170 (PubMed:12690205).Curated1
Sequence conflicti619L → P in EAL24170 (PubMed:12690205).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052970112Y → N. Corresponds to variant dbSNP:rs1476703Ensembl.1
Natural variantiVAR_052971575H → R. Corresponds to variant dbSNP:rs2041049Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018203228 – 234LYRPFYL → SPAVHLM in isoform 2. 1 Publication7
Alternative sequenceiVSP_018204235 – 674Missing in isoform 2. 1 PublicationAdd BLAST440

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028069 mRNA Translation: BAA78326.1
AB028070 mRNA Translation: BAA78327.1
AF160249 mRNA Translation: AAD41911.1
AF160876 mRNA Translation: AAD45357.1
AK292569 mRNA Translation: BAF85258.1
AC003083 Genomic DNA Translation: AAS07442.1
AC005164 Genomic DNA Translation: AAS07418.1
CH236949 Genomic DNA Translation: EAL24170.1
CH471091 Genomic DNA Translation: EAW76930.1
BC036045 mRNA Translation: AAH36045.1
BC047693 mRNA Translation: AAH47693.1
CCDSiCCDS5611.1 [Q9UBU7-1]
PIRiT02633
RefSeqiNP_001304989.1, NM_001318060.1
NP_001304990.1, NM_001318061.1
NP_001304991.1, NM_001318062.1
NP_006707.1, NM_006716.3 [Q9UBU7-1]
UniGeneiHs.485380

Genome annotation databases

EnsembliENST00000265728; ENSP00000265728; ENSG00000006634 [Q9UBU7-1]
ENST00000413643; ENSP00000414083; ENSG00000006634 [Q9UBU7-2]
GeneIDi10926
KEGGihsa:10926
UCSCiuc003ujf.1 human [Q9UBU7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDBF4A_HUMAN
AccessioniPrimary (citable) accession number: Q9UBU7
Secondary accession number(s): A4D1D8
, A8K954, O75226, Q75MS6, Q75N01, Q9Y2M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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