Q9UBS8 (RNF14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RNF14
EC=6.3.2.-

Alternative name(s):
Androgen receptor-associated protein 54
HFB30
RING finger protein 14
Triad2 protein

Gene names

Name:	RNF14
Synonyms:	ARA54
ORF Names:	HRIHFB2038

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. Ref.11
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity. Ref.11
Subcellular location	Cytoplasm. Nucleus Ref.8.
Tissue specificity	Widely expressed.
Domain	The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity. The RING-type zinc finger is essential for the interaction with UBE2E2.
Post-translational modification	RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
Sequence similarities	Belongs to the RBR family. RNF14 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers. Contains 1 RWD domain.
Caution	Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen receptor signaling pathway Non-traceable author statement Ref.9PubMed 15572661. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.11. Source: UniProtKB protein ubiquitination Inferred from expression pattern Ref.9. Source: UniProtKB regulation of androgen receptor signaling pathway Inferred from direct assay Ref.11. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.2. Source: UniProtKB response to estradiol stimulus Inferred from direct assay PubMed 15831516. Source: BHF-UCL transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.9. Source: UniProtKB nucleus Inferred from direct assay Ref.9. Source: UniProtKB
Molecular_function	small conjugating protein ligase activity Inferred from direct assay Ref.9. Source: UniProtKB transcription coactivator activity Non-traceable author statement PubMed 15572661. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AR	P10275	2	EBI-2130308,EBI-608057

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UBS8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UBS8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	E3 ubiquitin-protein ligase RNF14		PRO_0000056057
Regions
	Domain	11 – 137	127	RWD
	Zinc finger	220 – 270	51	RING-type 1; atypical
	Zinc finger	289 – 350	62	IBR-type
	Zinc finger	404 – 433	30	RING-type 2; degenerate
	Region	361 – 474	114	Interaction with androgen receptor
	Coiled coil	351 – 395	45	Potential
	Motif	37 – 45	9	D-box
Amino acid modifications
	Modified residue	348	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	1 – 126	126	Missing in isoform 2.		VSP_045780
Experimental info
	Mutagenesis	220	1	C → S: Loss of interaction with UBE2E2 and of autoubiquitination. Ref.9
	Sequence conflict	32	1	Q → R in CAG32983. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 529E3F5AF38A5DAD	FASTA	474	53,837
	10 20 30 40 50 60 MSSEDREAQE DELLALASIY DGDEFRKAES VQGGETRIYL DLPQNFKIFV SGNSNECLQN 70 80 90 100 110 120 SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP TQLSALCKHL DNLWEEHRGS 130 140 150 160 170 180 VVLFAWMQFL KEETLAYLNI VSPFELKIGS QKKVQRRTAQ ASPNTELDFG GAAGSDVDQE 190 200 210 220 230 240 EIVDERAVQD VESLSNLIQE ILDFDQAQQI KCFNSKLFLC SICFCEKLGS ECMYFLECRH 250 260 270 280 290 300 VYCKACLKDY FEIQIRDGQV QCLNCPEPKC PSVATPGQVK ELVEAELFAR YDRLLLQSSL 310 320 330 340 350 360 DLMADVVYCP RPCCQLPVMQ EPGCTMGICS SCNFAFCTLC RLTYHGVSPC KVTAEKLMDL 370 380 390 400 410 420 RNEYLQADEA NKRLLDQRYG KRVIQKALEE MESKEWLEKN SKSCPCCGTP IEKLDGCNKM 430 440 450 460 470 TCTGCMQYFC WICMGSLSRA NPYKHFNDPG SPCFNRLFYA VDVDDDIWED EVED
	Isoform 2 [UniParc]. Checksum: C43770874596DD0C	FASTA	348	39,634
	10 20 30 40 50 60 MQFLKEETLA YLNIVSPFEL KIGSQKKVQR RTAQASPNTE LDFGGAAGSD VDQEEIVDER 70 80 90 100 110 120 AVQDVESLSN LIQEILDFDQ AQQIKCFNSK LFLCSICFCE KLGSECMYFL ECRHVYCKAC 130 140 150 160 170 180 LKDYFEIQIR DGQVQCLNCP EPKCPSVATP GQVKELVEAE LFARYDRLLL QSSLDLMADV 190 200 210 220 230 240 VYCPRPCCQL PVMQEPGCTM GICSSCNFAF CTLCRLTYHG VSPCKVTAEK LMDLRNEYLQ 250 260 270 280 290 300 ADEANKRLLD QRYGKRVIQK ALEEMESKEW LEKNSKSCPC CGTPIEKLDG CNKMTCTGCM 310 320 330 340 QYFCWICMGS LSRANPYKHF NDPGSPCFNR LFYAVDVDDD IWEDEVED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel human gene (HFB30) which encodes a protein with a RING finger motif." Ueki N., Seki N., Yano K., Masuho Y., Saito T., Muramatsu M.-A. Biochim. Biophys. Acta 1445:232-236(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor." Kang H.-Y., Yeh S., Fujimoto N., Chang C. J. Biol. Chem. 274:8570-8576(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION. Tissue: Prostate.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Thyroid.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"Selection system for genes encoding nuclear-targeted proteins." Ueki N., Oda T., Kondo M., Yano K., Noguchi T., Muramatsu M.-A. Nat. Biotechnol. 16:1338-1342(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-474 (ISOFORM 1), SUBCELLULAR LOCATION. Tissue: Fetal brain.
[9]	"N-terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8." Ito K., Adachi S., Iwakami R., Yasuda H., Muto Y., Seki N., Okano Y. Eur. J. Biochem. 268:2725-2732(2001) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-220.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." Xu K., Shimelis H., Linn D.E., Jiang R., Yang X., Sun F., Guo Z., Chen H., Li W., Chen H., Kong X., Melamed J., Fang S., Xiao Z., Veenstra T.D., Qiu Y. Cancer Cell 15:270-282(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AR.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022663 mRNA. Translation: BAA78677.1. AF060544 mRNA. Translation: AAD21842.1. AK023884 mRNA. Translation: BAG51234.1. AK057868 mRNA. No translation available. CR456702 mRNA. Translation: CAG32983.1. AC005740 Genomic DNA. No translation available. CH471062 Genomic DNA. Translation: EAW61895.1. CH471062 Genomic DNA. Translation: EAW61896.1. CH471062 Genomic DNA. Translation: EAW61897.1. BC126185 mRNA. Translation: AAI26186.1. BC144061 mRNA. Translation: AAI44062.1. AB015333 mRNA. Translation: BAA34792.1.
IPI	IPI00003606. IPI00374140.
RefSeq	NP_001188294.1. NM_001201365.1. NP_004281.1. NM_004290.4. NP_899645.1. NM_183398.2. NP_899646.1. NM_183399.2. NP_899647.1. NM_183400.2. NP_899648.1. NM_183401.2.
UniGene	Hs.483616.
3D structure databases
ProteinModelPortal	Q9UBS8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UBS8. 13 interactions.
MINT	MINT-1187964.
STRING	9606.ENSP00000324956.
PTM databases
PhosphoSite	Q9UBS8.
Polymorphism databases
DMDM	17380293.
Proteomic databases
PaxDb	Q9UBS8.
PRIDE	Q9UBS8.
Protocols and materials databases
DNASU	9604.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000347642; ENSP00000324956; ENSG00000013561. ENST00000356143; ENSP00000348462; ENSG00000013561. ENST00000394514; ENSP00000378022; ENSG00000013561. ENST00000394519; ENSP00000378027; ENSG00000013561. ENST00000394520; ENSP00000378028; ENSG00000013561.
GeneID	9604.
KEGG	hsa:9604.
UCSC	uc003lly.3. human.
Organism-specific databases
CTD	9604.
GeneCards	GC05P141326.
HGNC	HGNC:10058. RNF14.
HPA	HPA008716.
MIM	605675. gene.
neXtProt	NX_Q9UBS8.
PharmGKB	PA34423.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG266709.
HOVERGEN	HBG057296.
InParanoid	Q9UBS8.
KO	K11971.
OMA	SQKKVQR.
PhylomeDB	Q9UBS8.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9UBS8.
Bgee	Q9UBS8.
CleanEx	HS_RNF14.
Genevestigator	Q9UBS8.
GermOnline	ENSG00000013561. Homo sapiens.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR006575. RWD-domain. IPR016135. UBQ-conjugating_enzyme/RWD. IPR002867. Znf_C6HC. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF01485. IBR. 2 hits. PF05773. RWD. 1 hit. [Graphical view]
SMART	SM00647. IBR. 2 hits. SM00184. RING. 1 hit. SM00591. RWD. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS50908. RWD. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RNF14. human.
GenomeRNAi	9604.
NextBio	36027.
SOURCE	Search...

Entry information

Entry name

RNF14_HUMAN

Accession

Primary (citable) accession number: Q9UBS8
Secondary accession number(s): A0AV26 , A6NMR2, A8MTW5, B3KN72, B7ZLV2, D3DQE4, O94793, Q6IBV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.