Q9UBS3 (DNJB9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily B member 9

Alternative name(s):
Endoplasmic reticulum DNA J domain-containing protein 4
Short name=ER-resident protein ERdj4
Short name=ERdj4
Microvascular endothelial differentiation gene 1 protein
Short name=Mdg-1

Gene names

Name:	DNAJB9
Synonyms:	MDG1
ORF Names:	UNQ743/PRO1471

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Acts as a co-chaperone with an Hsp70 protein. Ref.6
Subunit structure	Interacts with DERL1 By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.6. Source: UniProtKB activation of signaling protein activity involved in unfolded protein response Traceable author statement. Source: Reactome cellular protein metabolic process Traceable author statement. Source: Reactome endoplasmic reticulum unfolded protein response Traceable author statement. Source: Reactome
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from direct assay. Source: LIFEdb endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Traceable author statement. Source: Reactome extracellular vesicular exosome Inferred from direct assay PubMed 19199708. Source: UniProt nucleolus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	misfolded protein binding Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Chain

24 – 223

200

DnaJ homolog subfamily B member 9

PRO_0000071031

Regions

Domain

26 – 90

65

J

Natural variations

Natural variant

136

1

R → H.
Corresponds to variant rs17155937 [ dbSNP | Ensembl ].

VAR_048911

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

223

Helix Strand Turn

Details...

Helix

27 – 31

5

Helix

39 – 52

14

Turn

55 – 57

3

Helix

61 – 78

18

Helix

81 – 88

8

Helix

91 – 94

4

Beta strand

97 – 99

3

Sequences

	Sequence		Length	Mass (Da)
	Q9UBS3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 59E3E57FF8B378BC	FASTA	223	25,518
	10 20 30 40 50 60 MATPQSIFIF AICILMITEL ILASKSYYDI LGVPKSASER QIKKAFHKLA MKYHPDKNKS 70 80 90 100 110 120 PDAEAKFREI AEAYETLSDA NRRKEYDTLG HSAFTSGKGQ RGSGSSFEQS FNFNFDDLFK 130 140 150 160 170 180 DFGFFGQNQN TGSKKRFENH FQTRQDGGSS RQRHHFQEFS FGGGLFDDMF EDMEKMFSFS 190 200 210 220 GFDSTNQHTV QTENRFHGSS KHCRTVTQRR GNMVTTYTDC SGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human microvascular endothelial differentiation gene 1." Seki N., Hattori A., Muramatsu M., Miyajima N., Saito T. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C." Dong M., Bridges J.P., Apsley K., Xu Y., Weaver T.E. Mol. Biol. Cell 19:2620-2630(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Solution structure of J-domain from human DnaJ subfamily B member 9." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 24-102.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB026908 mRNA. Translation: BAA84703.1.
AF083247 mRNA. Translation: AAD39845.1.
AL080081 mRNA. Translation: CAB45701.1.
AY359045 mRNA. Translation: AAQ89404.1.
BC028912 mRNA. Translation: AAH28912.1.

PIR

T12472.

RefSeq

NP_036460.1. NM_012328.2.

UniGene

Hs.732494.
Hs.741182.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CTR	NMR	-	A	26-100	[»]

ProteinModelPortal

Q9UBS3.

SMR

Q9UBS3. Positions 27-105.

ModBase

Search...

MobiDB

Search...

Protein-protein interaction databases

BioGrid

110354. 16 interactions.

IntAct

Q9UBS3. 8 interactions.

MINT

MINT-1375508.

STRING

9606.ENSP00000249356.

PTM databases

PhosphoSite

Q9UBS3.

Polymorphism databases

DMDM

18203496.

Proteomic databases

PaxDb

Q9UBS3.

PRIDE

Q9UBS3.

Protocols and materials databases

DNASU

4189.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000249356; ENSP00000249356; ENSG00000128590.

GeneID

4189.

KEGG

hsa:4189.

UCSC

uc003vfn.3. human.

Organism-specific databases

CTD

4189.

GeneCards

GC07P108210.

HGNC

HGNC:6968. DNAJB9.

HPA

HPA040967.
HPA041553.

MIM

602634. gene.

neXtProt

NX_Q9UBS3.

PharmGKB

PA27419.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0484.

HOGENOM

HOG000290187.

HOVERGEN

HBG051372.

InParanoid

Q9UBS3.

KO

K09515.

OMA

ATPQSIF.

OrthoDB

EOG71CFND.

PhylomeDB

Q9UBS3.

TreeFam

TF105143.

Enzyme and pathway databases

Reactome

REACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpress

Q9UBS3.

Bgee

Q9UBS3.

CleanEx

HS_DNAJB9.

Genevestigator

Q9UBS3.

Family and domain databases

Gene3D

1.10.287.110. 1 hit.

InterPro

IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]

Pfam

PF00226. DnaJ. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. SSF46565. 1 hit.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DNAJB9. human.

EvolutionaryTrace

Q9UBS3.

GeneWiki

DNAJB9.

GenomeRNAi

4189.

NextBio

16506.

PRO

Q9UBS3.

SOURCE

Search...

Entry information

Entry name

DNJB9_HUMAN

Accession

Primary (citable) accession number: Q9UBS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	May 1, 2000
Last modified:	April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.