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Protein

DnaJ homolog subfamily B member 9

Gene

DNAJB9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Acts as a co-chaperone with an Hsp70 protein.1 Publication

GO - Molecular functioni

  1. misfolded protein binding Source: UniProtKB

GO - Biological processi

  1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
  2. cellular protein metabolic process Source: Reactome
  3. endoplasmic reticulum unfolded protein response Source: Reactome
  4. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 9
Alternative name(s):
Endoplasmic reticulum DNA J domain-containing protein 4
Short name:
ER-resident protein ERdj4
Short name:
ERdj4
Microvascular endothelial differentiation gene 1 protein
Short name:
Mdg-1
Gene namesi
Name:DNAJB9
Synonyms:MDG1
ORF Names:UNQ743/PRO1471
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:6968. DNAJB9.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  4. endoplasmic reticulum membrane Source: Reactome
  5. extracellular vesicular exosome Source: UniProtKB
  6. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27419.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 223200DnaJ homolog subfamily B member 9PRO_0000071031Add
BLAST

Proteomic databases

MaxQBiQ9UBS3.
PaxDbiQ9UBS3.
PRIDEiQ9UBS3.

PTM databases

PhosphoSiteiQ9UBS3.

Expressioni

Gene expression databases

BgeeiQ9UBS3.
CleanExiHS_DNAJB9.
ExpressionAtlasiQ9UBS3. baseline and differential.
GenevestigatoriQ9UBS3.

Organism-specific databases

HPAiHPA040967.
HPA041553.

Interactioni

Subunit structurei

Interacts with DERL1.By similarity

Protein-protein interaction databases

BioGridi110354. 28 interactions.
IntActiQ9UBS3. 8 interactions.
MINTiMINT-1375508.
STRINGi9606.ENSP00000249356.

Structurei

Secondary structure

1
223
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi27 – 315Combined sources
Helixi39 – 5214Combined sources
Turni55 – 573Combined sources
Helixi61 – 7818Combined sources
Helixi81 – 888Combined sources
Helixi91 – 944Combined sources
Beta strandi97 – 993Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CTRNMR-A26-100[»]
ProteinModelPortaliQ9UBS3.
SMRiQ9UBS3. Positions 27-105.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBS3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 9065JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000290187.
HOVERGENiHBG051372.
InParanoidiQ9UBS3.
KOiK09515.
OMAiATPQSIF.
OrthoDBiEOG71CFND.
PhylomeDBiQ9UBS3.
TreeFamiTF105143.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UBS3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATPQSIFIF AICILMITEL ILASKSYYDI LGVPKSASER QIKKAFHKLA
60 70 80 90 100
MKYHPDKNKS PDAEAKFREI AEAYETLSDA NRRKEYDTLG HSAFTSGKGQ
110 120 130 140 150
RGSGSSFEQS FNFNFDDLFK DFGFFGQNQN TGSKKRFENH FQTRQDGGSS
160 170 180 190 200
RQRHHFQEFS FGGGLFDDMF EDMEKMFSFS GFDSTNQHTV QTENRFHGSS
210 220
KHCRTVTQRR GNMVTTYTDC SGQ
Length:223
Mass (Da):25,518
Last modified:May 1, 2000 - v1
Checksum:i59E3E57FF8B378BC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti136 – 1361R → H.
Corresponds to variant rs17155937 [ dbSNP | Ensembl ].
VAR_048911

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026908 mRNA. Translation: BAA84703.1.
AF083247 mRNA. Translation: AAD39845.1.
AL080081 mRNA. Translation: CAB45701.1.
AY359045 mRNA. Translation: AAQ89404.1.
BC028912 mRNA. Translation: AAH28912.1.
CCDSiCCDS5752.1.
PIRiT12472.
RefSeqiNP_036460.1. NM_012328.2.
UniGeneiHs.732494.
Hs.741182.

Genome annotation databases

EnsembliENST00000249356; ENSP00000249356; ENSG00000128590.
GeneIDi4189.
KEGGihsa:4189.
UCSCiuc003vfn.3. human.

Polymorphism databases

DMDMi18203496.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026908 mRNA. Translation: BAA84703.1.
AF083247 mRNA. Translation: AAD39845.1.
AL080081 mRNA. Translation: CAB45701.1.
AY359045 mRNA. Translation: AAQ89404.1.
BC028912 mRNA. Translation: AAH28912.1.
CCDSiCCDS5752.1.
PIRiT12472.
RefSeqiNP_036460.1. NM_012328.2.
UniGeneiHs.732494.
Hs.741182.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CTRNMR-A26-100[»]
ProteinModelPortaliQ9UBS3.
SMRiQ9UBS3. Positions 27-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110354. 28 interactions.
IntActiQ9UBS3. 8 interactions.
MINTiMINT-1375508.
STRINGi9606.ENSP00000249356.

PTM databases

PhosphoSiteiQ9UBS3.

Polymorphism databases

DMDMi18203496.

Proteomic databases

MaxQBiQ9UBS3.
PaxDbiQ9UBS3.
PRIDEiQ9UBS3.

Protocols and materials databases

DNASUi4189.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249356; ENSP00000249356; ENSG00000128590.
GeneIDi4189.
KEGGihsa:4189.
UCSCiuc003vfn.3. human.

Organism-specific databases

CTDi4189.
GeneCardsiGC07P108210.
HGNCiHGNC:6968. DNAJB9.
HPAiHPA040967.
HPA041553.
MIMi602634. gene.
neXtProtiNX_Q9UBS3.
PharmGKBiPA27419.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000290187.
HOVERGENiHBG051372.
InParanoidiQ9UBS3.
KOiK09515.
OMAiATPQSIF.
OrthoDBiEOG71CFND.
PhylomeDBiQ9UBS3.
TreeFamiTF105143.

Enzyme and pathway databases

ReactomeiREACT_18273. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiDNAJB9. human.
EvolutionaryTraceiQ9UBS3.
GeneWikiiDNAJB9.
GenomeRNAii4189.
NextBioi16506.
PROiQ9UBS3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UBS3.
CleanExiHS_DNAJB9.
ExpressionAtlasiQ9UBS3. baseline and differential.
GenevestigatoriQ9UBS3.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human microvascular endothelial differentiation gene 1."
    Seki N., Hattori A., Muramatsu M., Miyajima N., Saito T.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C."
    Dong M., Bridges J.P., Apsley K., Xu Y., Weaver T.E.
    Mol. Biol. Cell 19:2620-2630(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Solution structure of J-domain from human DnaJ subfamily B member 9."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 24-102.

Entry informationi

Entry nameiDNJB9_HUMAN
AccessioniPrimary (citable) accession number: Q9UBS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.