Reviewed, UniProtKB/Swiss-Prot Q9UBS0 (KS6B2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ribosomal protein S6 kinase beta-2
      Short name=S6K-beta 2
      Short name=S6K2
    EC=2.7.11.1
Alternative name(s):
    70 kDa ribosomal protein S6 kinase 2
    p70 ribosomal S6 kinase beta
      Short name=p70 S6 kinase beta
      Short name=p70 S6Kbeta
      Short name=p70-S6KB
      Short name=S6K-beta
      Short name=p70-beta
    S6 kinase-related kinase
      Short name=SRK
    Serine/threonine-protein kinase 14 beta

Gene names

Name:	RPS6KB2
Synonyms:	STK14B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates specifically ribosomal protein S6.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	translation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Ribosomal protein S6 kinase beta-2		PRO_0000086214
Regions
	Domain	67 – 328	262	Protein kinase
	Domain	329 – 399	71	AGC-kinase C-terminal
	Nucleotide binding	73 – 81	9	ATP By similarity
	Compositional bias	411 – 482	72	Pro-rich
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	99	1	ATP By similarity
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.6
	Modified residue	356	1	Phosphoserine Ref.7
Natural variations
	Natural variant	267	1	P → L Ref.9		VAR_040643
	Natural variant	368	1	V → M Ref.9		VAR_040644
	Natural variant	420	1	V → A Ref.9 Ref.2		VAR_040645
	Natural variant	443	1	T → M in an ovarian mucinous carcinoma sample; somatic mutation. Ref.9		VAR_040646
Experimental info
	Sequence conflict	90	1	Missing in BAA37145. Ref.2
	Sequence conflict	409	1	R → C in BAA37145. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UBS0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5D2C691D24A2D0A9	FASTA	482	53,483
	10 20 30 40 50 60 MAAVFDLDLE TEEGSEGEGE PELSPADACP LAELRAAGLE PVGHYEEVEL TETSVNVGPE 70 80 90 100 110 120 RIGPHCFELL RVLGKGGYGK VFQVRKVQGT NLGKIYAMKV LRKAKIVRNA KDTAHTRAER 130 140 150 160 170 180 NILESVKHPF IVELAYAFQT GGKLYLILEC LSGGELFTHL EREGIFLEDT ACFYLAEITL 190 200 210 220 230 240 ALGHLHSQGI IYRDLKPENI MLSSQGHIKL TDFGLCKESI HEGAVTHTFC GTIEYMAPEI 250 260 270 280 290 300 LVRSGHNRAV DWWSLGALMY DMLTGSPPFT AENRKKTMDK IIRGKLALPP YLTPDARDLV 310 320 330 340 350 360 KKFLKRNPSQ RIGGGPGDAA DVQRHPFFRH MNWDDLLAWR VDPPFRPCLQ SEEDVSQFDT 370 380 390 400 410 420 RFTRQTPVDS PDDTALSESA NQAFLGFTYV APSVLDSIKE GFSFQPKLRS PRRLNSSPRV 430 440 450 460 470 480 PVSPLKFSPF EGFRPSPSLP EPTELPLPPL LPPPPPSTTA PLPIRPPSGT KKSKRGRGRP GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." Gout I., Minami T., Hara K., Tsujishita Y., Filonenko V., Waterfield M.D., Yonezawa K. J. Biol. Chem. 273:30061-30064(1998) [PubMed: 9804755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of p70 S6Kbeta defines a novel family of p70 S6 kinases." Saitoh M., ten Dijke P., Miyazono K., Ichijo H. Biochem. Biophys. Res. Commun. 253:470-476(1998) [PubMed: 9878560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-420.
[3]	"Characterization of S6K2, a novel kinase homologous to S6K1." Lee-Fruman K.K., Kuo C.J., Lippincott J., Terada N., Blenis J. Oncogene 18:5108-5114(1999) [PubMed: 10490847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and characterization of a novel S6 kinase-related kinase, SRK." Koh H.J., Lee B.N., Choi H.S., Chung J. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-267; MET-368; ALA-420 AND MET-443.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB016869 mRNA. Translation: BAA34402.1. Different initiation. AB019245 mRNA. Translation: BAA37145.1. AF076931 mRNA. Translation: AAD46063.1. AF099739 mRNA. Translation: AAD20990.1. BC000094 mRNA. Translation: AAH00094.3.
IPI	IPI00217069.
PIR	JE0377.
RefSeq	NP_003943.2.
UniGene	Hs.534345
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
PTM databases
PhosphoSite	Q9UBS0.
Proteomic databases
PRIDE	Q9UBS0.
Genome annotation databases
Ensembl	ENSG00000175634. Homo sapiens. [Contig view]
GeneID	6199.
KEGG	hsa:6199.
Organism-specific databases
GeneCards	GC11P066952.
H-InvDB	HIX0009859.
HGNC	HGNC:10437. RPS6KB2.
HPA	CAB019404. HPA010010.
MIM	608939. gene.
PharmGKB	PA34852.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9UBS0.
HOVERGEN	Q9UBS0.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Reactome	REACT_11061. Signalling by NGF.
Gene expression databases
Bgee	Q9UBS0.
CleanEx	HS_RPS6KB2.
GermOnline	ENSG00000175634. Homo sapiens.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR017892. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR016238. Ribosomal_S6_kinase. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000605. Ribsml_S6_kin_1. 1 hit.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	24077.
SOURCE	Search...

Entry information

Entry name

KS6B2_HUMAN

Accession

Primary (citable) accession number: Q9UBS0
Secondary accession number(s): O94809, Q9UEC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)