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Protein

Chemokine-like factor

Gene

CKLF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10.1 Publication
Isoform 1: has chemotactic response in monocytes, neutrophils and lymphocytes (PubMed:11415443). Binds CCR4 (PubMed:16137713).2 Publications

GO - Molecular functioni

  • chemokine activity Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • lymphocyte chemotaxis Source: UniProtKB
  • macrophage chemotaxis Source: UniProtKB
  • neutrophil chemotaxis Source: UniProtKB
  • secretion by cell Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Chemokine-like factor
Alternative name(s):
C32
Gene namesi
Name:CKLF
Synonyms:CKLF1
ORF Names:HSPC224, UNQ410/PRO772
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:13253. CKLF.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei45 – 6521HelicalSequence analysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Transmembranei108 – 12821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell Source: GOC
  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26531.

Polymorphism and mutation databases

BioMutaiCKLF.
DMDMi34098753.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Chemokine-like factorPRO_0000186094Add
BLAST

Proteomic databases

MaxQBiQ9UBR5.
PaxDbiQ9UBR5.
PRIDEiQ9UBR5.

PTM databases

iPTMnetiQ9UBR5.
PhosphoSiteiQ9UBR5.

Expressioni

Tissue specificityi

Isoform 1, isoform 2, isoform 3 and isoform 4 have highest expression levels in adult spleen, lung, testis, ovary, peripheral blood leukocyte, placenta, pancreas, and in fetal brain, skeletal muscle, thymus and heart. Lower expression levels in adult skeletal muscle, liver, thymus colon, prostate and fetal spleen and liver.1 Publication

Gene expression databases

BgeeiQ9UBR5.
CleanExiHS_CKLF.
ExpressionAtlasiQ9UBR5. baseline and differential.
GenevisibleiQ9UBR5. HS.

Interactioni

GO - Molecular functioni

  • chemokine activity Source: UniProtKB

Protein-protein interaction databases

BioGridi119366. 2 interactions.
STRINGi9606.ENSP00000264001.

Structurei

3D structure databases

ProteinModelPortaliQ9UBR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 133121MARVELPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the chemokine-like factor family.Curated
Contains 1 MARVEL domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4788. Eukaryota.
ENOG4111IAF. LUCA.
GeneTreeiENSGT00520000055675.
HOGENOMiHOG000111752.
HOVERGENiHBG039393.
InParanoidiQ9UBR5.
OMAiLYIFRLD.
OrthoDBiEOG7MPRGN.
PhylomeDBiQ9UBR5.
TreeFamiTF317387.

Family and domain databases

InterProiIPR008253. Marvel.
[Graphical view]
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q9UBR5-1) [UniParc]FASTAAdd to basket

Also known as: CKLF21 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDNVQPKIKH RPFCFSVKGH VKMLRLALTV TSMTFFIIAQ APEPYIVITG
60 70 80 90 100
FEVTVILFFI LLYVLRLDRL MKWLFWPLLD IINSLVTTVF MLIVSVLALI
110 120 130 140 150
PETTTLTVGG GVFALVTAVC CLADGALIYR KLLFNPSGPY QKKPVHEKKE

VL
Length:152
Mass (Da):17,170
Last modified:May 1, 2000 - v1
Checksum:i6149D6D2B6AED9F3
GO
Isoform 1 (identifier: Q9UBR5-2) [UniParc]FASTAAdd to basket

Also known as: CKLF11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     27-79: Missing.

Show »
Length:99
Mass (Da):10,923
Checksum:i1F5EBF5AA7A9479E
GO
Isoform 3 (identifier: Q9UBR5-3) [UniParc]FASTAAdd to basket

Also known as: CKLF31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     27-79: Missing.
     80-111: Missing.

Show »
Length:67
Mass (Da):7,652
Checksum:i7BEB754D8880F1F9
GO
Isoform 4 (identifier: Q9UBR5-4) [UniParc]FASTAAdd to basket

Also known as: CKLF41 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     80-111: Missing.

Show »
Length:120
Mass (Da):13,899
Checksum:iBF0C011077F24C13
GO
Isoform 5 (identifier: Q9UBR5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-113: VF → LF
     114-152: Missing.

Show »
Length:113
Mass (Da):12,888
Checksum:iE48166E6D332DC75
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei27 – 7953Missing in isoform 1 and isoform 3. 1 PublicationVSP_050605Add
BLAST
Alternative sequencei80 – 11132Missing in isoform 4 and isoform 3. 1 PublicationVSP_050606Add
BLAST
Alternative sequencei112 – 1132VF → LF in isoform 5. CuratedVSP_041445
Alternative sequencei114 – 15239Missing in isoform 5. CuratedVSP_041446Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135380 mRNA. Translation: AAF19599.1.
AF135381 mRNA. Translation: AAF19600.1.
AF145216 mRNA. Translation: AAF19350.1.
AF096895 mRNA. Translation: AAF06722.1.
AF057306 mRNA. Translation: AAF21255.1.
AF151058 mRNA. Translation: AAF36144.1.
AY358999 mRNA. Translation: AAQ89358.1.
AY665769 Genomic DNA. Translation: AAT70694.1.
AC010542 Genomic DNA. No translation available.
BC004380 mRNA. Translation: AAH04380.1.
CCDSiCCDS10806.1. [Q9UBR5-3]
CCDS10807.1. [Q9UBR5-1]
CCDS10808.1. [Q9UBR5-2]
CCDS10809.1. [Q9UBR5-4]
CCDS45502.1. [Q9UBR5-5]
RefSeqiNP_001035228.1. NM_001040138.2. [Q9UBR5-5]
NP_057410.1. NM_016326.3. [Q9UBR5-3]
NP_058647.1. NM_016951.3. [Q9UBR5-1]
NP_857591.1. NM_181640.2. [Q9UBR5-2]
NP_857592.1. NM_181641.2. [Q9UBR5-4]
UniGeneiHs.15159.
Hs.698621.

Genome annotation databases

EnsembliENST00000264001; ENSP00000264001; ENSG00000217555. [Q9UBR5-1]
ENST00000345436; ENSP00000290771; ENSG00000217555. [Q9UBR5-4]
ENST00000351137; ENSP00000264003; ENSG00000217555. [Q9UBR5-2]
ENST00000362093; ENSP00000355417; ENSG00000217555. [Q9UBR5-3]
ENST00000417030; ENSP00000416678; ENSG00000217555. [Q9UBR5-5]
GeneIDi51192.
KEGGihsa:51192.
UCSCiuc002eow.4. human. [Q9UBR5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135380 mRNA. Translation: AAF19599.1.
AF135381 mRNA. Translation: AAF19600.1.
AF145216 mRNA. Translation: AAF19350.1.
AF096895 mRNA. Translation: AAF06722.1.
AF057306 mRNA. Translation: AAF21255.1.
AF151058 mRNA. Translation: AAF36144.1.
AY358999 mRNA. Translation: AAQ89358.1.
AY665769 Genomic DNA. Translation: AAT70694.1.
AC010542 Genomic DNA. No translation available.
BC004380 mRNA. Translation: AAH04380.1.
CCDSiCCDS10806.1. [Q9UBR5-3]
CCDS10807.1. [Q9UBR5-1]
CCDS10808.1. [Q9UBR5-2]
CCDS10809.1. [Q9UBR5-4]
CCDS45502.1. [Q9UBR5-5]
RefSeqiNP_001035228.1. NM_001040138.2. [Q9UBR5-5]
NP_057410.1. NM_016326.3. [Q9UBR5-3]
NP_058647.1. NM_016951.3. [Q9UBR5-1]
NP_857591.1. NM_181640.2. [Q9UBR5-2]
NP_857592.1. NM_181641.2. [Q9UBR5-4]
UniGeneiHs.15159.
Hs.698621.

3D structure databases

ProteinModelPortaliQ9UBR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119366. 2 interactions.
STRINGi9606.ENSP00000264001.

PTM databases

iPTMnetiQ9UBR5.
PhosphoSiteiQ9UBR5.

Polymorphism and mutation databases

BioMutaiCKLF.
DMDMi34098753.

Proteomic databases

MaxQBiQ9UBR5.
PaxDbiQ9UBR5.
PRIDEiQ9UBR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264001; ENSP00000264001; ENSG00000217555. [Q9UBR5-1]
ENST00000345436; ENSP00000290771; ENSG00000217555. [Q9UBR5-4]
ENST00000351137; ENSP00000264003; ENSG00000217555. [Q9UBR5-2]
ENST00000362093; ENSP00000355417; ENSG00000217555. [Q9UBR5-3]
ENST00000417030; ENSP00000416678; ENSG00000217555. [Q9UBR5-5]
GeneIDi51192.
KEGGihsa:51192.
UCSCiuc002eow.4. human. [Q9UBR5-1]

Organism-specific databases

CTDi51192.
GeneCardsiCKLF.
HGNCiHGNC:13253. CKLF.
MIMi616074. gene.
neXtProtiNX_Q9UBR5.
PharmGKBiPA26531.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4788. Eukaryota.
ENOG4111IAF. LUCA.
GeneTreeiENSGT00520000055675.
HOGENOMiHOG000111752.
HOVERGENiHBG039393.
InParanoidiQ9UBR5.
OMAiLYIFRLD.
OrthoDBiEOG7MPRGN.
PhylomeDBiQ9UBR5.
TreeFamiTF317387.

Miscellaneous databases

ChiTaRSiCKLF. human.
GeneWikiiCKLF_(gene).
GenomeRNAii51192.
NextBioi54186.
PROiQ9UBR5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UBR5.
CleanExiHS_CKLF.
ExpressionAtlasiQ9UBR5. baseline and differential.
GenevisibleiQ9UBR5. HS.

Family and domain databases

InterProiIPR008253. Marvel.
[Graphical view]
PROSITEiPS51225. MARVEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity."
    Han W., Lou Y., Tang J., Zhang Y., Chen Y., Li Y., Gu W., Huang J., Gui L., Tang Y., Li F., Song Q., Di C., Wang L., Shi Q., Sun R., Xia D., Rui M., Tang J., Ma D.
    Biochem. J. 357:127-135(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Zhang J.S., Nelson M., Wang L., Smith D.I.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: PancreasCurated.
  3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Umbilical cord blood.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. SeattleSNPs variation discovery resource
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: PancreasImported.
  8. "Chemokine-like factor 1 is a functional ligand for CC chemokine receptor 4 (CCR4)."
    Wang Y., Zhang Y., Yang X., Han W., Liu Y., Xu Q., Zhao R., Di C., Song Q., Ma D.
    Life Sci. 78:614-621(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION (ISOFORM 1), FUNCTION (ISOFORM 1).

Entry informationi

Entry nameiCKLF_HUMAN
AccessioniPrimary (citable) accession number: Q9UBR5
Secondary accession number(s): C9JE38
, Q9UHM7, Q9UHN8, Q9UI41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: May 1, 2000
Last modified: March 16, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.