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Protein

Eukaryotic translation initiation factor 3 subunit K

Gene

EIF3K

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniRule annotation3 Publications

GO - Molecular functioni

GO - Biological processi

  • regulation of translational initiation Source: InterPro
  • translational initiation Source: UniProtKB

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit KUniRule annotation
Short name:
eIF3kUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 12UniRule annotation
Muscle-specific gene M9 protein
PLAC-24
eIF-3 p25UniRule annotation
eIF-3 p28
Gene namesi
Name:EIF3KUniRule annotation
Synonyms:EIF3S12UniRule annotation
ORF Names:ARG134, HSPC029, MSTP001, PTD001
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000178982.9
HGNCiHGNC:24656 EIF3K
MIMi609596 gene
neXtProtiNX_Q9UBQ5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi27335
OpenTargetsiENSG00000178982
PharmGKBiPA162384923

Polymorphism and mutation databases

BioMutaiEIF3K
DMDMi23396628

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotationCombined sources2 Publications
ChainiPRO_00001235462 – 218Eukaryotic translation initiation factor 3 subunit KAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationCombined sources2 Publications1
Modified residuei28PhosphothreonineCombined sources1
Modified residuei217PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UBQ5
PaxDbiQ9UBQ5
PeptideAtlasiQ9UBQ5
PRIDEiQ9UBQ5
TopDownProteomicsiQ9UBQ5-1 [Q9UBQ5-1]

PTM databases

iPTMnetiQ9UBQ5
PhosphoSitePlusiQ9UBQ5
SwissPalmiQ9UBQ5

Expressioni

Tissue specificityi

Ubiquitous, with the highest levels of expression in brain, testis and kidney.1 Publication

Gene expression databases

BgeeiENSG00000178982
CleanExiHS_EIF3K
ExpressionAtlasiQ9UBQ5 baseline and differential
GenevisibleiQ9UBQ5 HS

Organism-specific databases

HPAiHPA045446
HPA054590

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with CCND3, but not with CCND1 and CCND2.UniRule annotation6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi118148, 61 interactors
CORUMiQ9UBQ5
DIPiDIP-32880N
IntActiQ9UBQ5, 25 interactors
MINTiQ9UBQ5
STRINGi9606.ENSP00000248342

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi18 – 21Combined sources4
Helixi23 – 25Combined sources3
Helixi26 – 39Combined sources14
Helixi44 – 56Combined sources13
Helixi58 – 60Combined sources3
Helixi63 – 75Combined sources13
Turni76 – 78Combined sources3
Helixi81 – 87Combined sources7
Helixi91 – 94Combined sources4
Helixi99 – 110Combined sources12
Helixi114 – 120Combined sources7
Helixi126 – 129Combined sources4
Helixi134 – 149Combined sources16
Beta strandi151 – 153Combined sources3
Helixi155 – 161Combined sources7
Helixi167 – 177Combined sources11
Helixi192 – 195Combined sources4
Helixi207 – 214Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RZ4X-ray2.10A1-218[»]
3J8Belectron microscopy-K1-190[»]
3J8Celectron microscopy-K1-190[»]
6FECelectron microscopy6.3061-218[»]
ProteinModelPortaliQ9UBQ5
SMRiQ9UBQ5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBQ5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 204PCIPROSITE-ProRule annotationAdd BLAST163

Sequence similaritiesi

Belongs to the eIF-3 subunit K family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3252 Eukaryota
ENOG410ZSE4 LUCA
GeneTreeiENSGT00390000009409
HOGENOMiHOG000008222
HOVERGENiHBG052081
InParanoidiQ9UBQ5
KOiK15028
OMAiYVQDQAK
OrthoDBiEOG091G0IXT
PhylomeDBiQ9UBQ5
TreeFamiTF314893

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.25.40.250, 1 hit
HAMAPiMF_03010 eIF3k, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR033464 CSN8_PSD8_EIF3K
IPR009374 eIF3k
IPR000717 PCI_dom
IPR016020 Transl_init_fac_sub12_N_euk
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13022 PTHR13022, 1 hit
PfamiView protein in Pfam
PF10075 CSN8_PSD8_EIF3K, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV
60 70 80 90 100
LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR
110 120 130 140 150
QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY
160 170 180 190 200
QHIDRWLLAE MLGDLSDSQL KVWMSKYGWS ADESGQIFIC SQEESIKPKN
210
IVEKIDFDSV SSIMASSQ
Length:218
Mass (Da):25,060
Last modified:May 1, 2000 - v1
Checksum:i6B2CBBE8A9D1F28F
GO
Isoform 2 (identifier: Q9UBQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-218: VSSIMASSQ → EW

Note: No experimental confirmation available.
Show »
Length:211
Mass (Da):24,484
Checksum:i59CDE70AC1995450
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119Q → K in AAD40193 (PubMed:11042152).Curated1

Mass spectrometryi

Molecular mass is 24970.6 Da from positions 1 - 218. 1 Publication
Molecular mass is 24971.1±0.2 Da from positions 1 - 218. Determined by MALDI. 1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055475210 – 218VSSIMASSQ → EW in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY245432 mRNA Translation: AAP22070.1
AB019392 mRNA Translation: BAA76626.1
AF077051 mRNA Translation: AAD27784.1
AF109355 mRNA Translation: AAQ13503.1
AF315506 mRNA Translation: AAK01365.1
AF085358 mRNA Translation: AAD40193.1
AK289554 mRNA Translation: BAF82243.1
AK316344 mRNA Translation: BAH14715.1
AC008649 Genomic DNA No translation available.
CH471126 Genomic DNA Translation: EAW56807.1
BC001031 mRNA Translation: AAH01031.1
BC007335 mRNA Translation: AAH07335.2
BC007559 mRNA Translation: AAH07559.1
CCDSiCCDS12517.1 [Q9UBQ5-1]
RefSeqiNP_037366.1, NM_013234.3 [Q9UBQ5-1]
UniGeneiHs.314359

Genome annotation databases

EnsembliENST00000248342; ENSP00000248342; ENSG00000178982 [Q9UBQ5-1]
ENST00000545173; ENSP00000438145; ENSG00000178982 [Q9UBQ5-2]
ENST00000635417; ENSP00000489379; ENSG00000282986 [Q9UBQ5-2]
ENST00000635567; ENSP00000489438; ENSG00000282986 [Q9UBQ5-1]
GeneIDi27335
KEGGihsa:27335
UCSCiuc002oiz.2 human [Q9UBQ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEIF3K_HUMAN
AccessioniPrimary (citable) accession number: Q9UBQ5
Secondary accession number(s): A8K0I9
, B7ZAM9, Q96IQ0, Q9Y6D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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