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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

METTL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei160S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Active sitei163UniRule annotation1

GO - Molecular functioni

  • tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
  • tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS00526-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.
SIGNORiQ9UBP6.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:METTL1UniRule annotation
Synonyms:C12orf1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:7030. METTL1.

Subcellular locationi

  • Nucleus UniRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleolus Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • tRNA methyltransferase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27S → A: Able to complement a trm8-delta mutant yeast. 1 Publication1
Mutagenesisi27S → D or E: Abolishes ability to complement a trm8-delta mutant yeast. 1 Publication1

Organism-specific databases

DisGeNETi4234.
OpenTargetsiENSG00000037897.
PharmGKBiPA30766.

Polymorphism and mutation databases

BioMutaiMETTL1.
DMDMi32171926.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001714312 – 276tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei27Phosphoserine; by PKB and RPS6KA3UniRule annotationCombined sources1 Publication1

Post-translational modificationi

Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UBP6.
PaxDbiQ9UBP6.
PeptideAtlasiQ9UBP6.
PRIDEiQ9UBP6.

PTM databases

iPTMnetiQ9UBP6.
PhosphoSitePlusiQ9UBP6.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000037897.
CleanExiHS_METTL1.
ExpressionAtlasiQ9UBP6. baseline and differential.
GenevisibleiQ9UBP6. HS.

Organism-specific databases

HPAiHPA020914.
HPA050450.

Interactioni

Subunit structurei

Forms a complex with WDR4.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
WDR4P570814EBI-750415,EBI-750427

Protein-protein interaction databases

BioGridi110392. 37 interactors.
DIPiDIP-61920N.
IntActiQ9UBP6. 1 interactor.
MINTiMINT-1440834.
STRINGi9606.ENSP00000314441.

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 43Combined sources3
Turni47 – 49Combined sources3
Beta strandi78 – 83Combined sources6
Helixi89 – 94Combined sources6
Helixi95 – 97Combined sources3
Beta strandi101 – 108Combined sources8
Helixi110 – 125Combined sources16
Beta strandi134 – 138Combined sources5
Turni141 – 143Combined sources3
Helixi145 – 148Combined sources4
Beta strandi154 – 161Combined sources8
Helixi178 – 187Combined sources10
Beta strandi188 – 199Combined sources12
Helixi201 – 212Combined sources12
Beta strandi217 – 220Combined sources4
Helixi222 – 225Combined sources4
Helixi231 – 233Combined sources3
Turni234 – 236Combined sources3
Helixi239 – 246Combined sources8
Beta strandi252 – 258Combined sources7
Turni262 – 264Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKKX-ray1.55A32-265[»]
ProteinModelPortaliQ9UBP6.
SMRiQ9UBP6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBP6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108S-adenosyl-L-methionine binding2
Regioni140 – 141S-adenosyl-L-methionine binding2
Regioni238 – 240S-adenosyl-L-methionine binding3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000169646.
HOVERGENiHBG044569.
InParanoidiQ9UBP6.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG091G0IQ7.
PhylomeDBiQ9UBP6.
TreeFamiTF314083.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY
60 70 80 90 100
PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD
110 120 130 140 150
TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY
160 170 180 190 200
KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV
210 220 230 240 250
LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK
260 270
NFPAIFRRIQ DPVLQAVTSQ TSLPGH
Length:276
Mass (Da):31,471
Last modified:May 1, 2000 - v1
Checksum:i715AE85A18632892
GO
Isoform 2 (identifier: Q9UBP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-155: VELSPLFPDT...PNFFYKGQLT → ADKDVLPLPR...VHSFRRAPTV
     156-276: Missing.

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,423
Checksum:iCFC2A730BA85E8D4
GO

Sequence cautioni

The sequence CAA65470 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18K → R in BAD96922 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04467192 – 155VELSP…KGQLT → ADKDVLPLPRPTFQADKAQV ANHQSHPASRICLRAKSWGA GVYHNRCAGATRLDVHSFRR APTV in isoform 2. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_044672156 – 276Missing in isoform 2. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18643 mRNA. Translation: CAA77239.1.
Y18642 Genomic DNA. Translation: CAA77238.1.
X96698 mRNA. Translation: CAA65470.1. Different initiation.
AK314851 mRNA. Translation: BAG37368.1.
AK223202 mRNA. Translation: BAD96922.1.
AC025165 Genomic DNA. No translation available.
BC000550 mRNA. Translation: AAH00550.1.
CCDSiCCDS8955.3. [Q9UBP6-1]
CCDS8956.1. [Q9UBP6-2]
RefSeqiNP_005362.3. NM_005371.5. [Q9UBP6-1]
NP_075422.3. NM_023033.3. [Q9UBP6-2]
UniGeneiHs.42957.

Genome annotation databases

EnsembliENST00000257848; ENSP00000257848; ENSG00000037897. [Q9UBP6-2]
ENST00000324871; ENSP00000314441; ENSG00000037897. [Q9UBP6-1]
GeneIDi4234.
KEGGihsa:4234.
UCSCiuc009zqc.4. human. [Q9UBP6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18643 mRNA. Translation: CAA77239.1.
Y18642 Genomic DNA. Translation: CAA77238.1.
X96698 mRNA. Translation: CAA65470.1. Different initiation.
AK314851 mRNA. Translation: BAG37368.1.
AK223202 mRNA. Translation: BAD96922.1.
AC025165 Genomic DNA. No translation available.
BC000550 mRNA. Translation: AAH00550.1.
CCDSiCCDS8955.3. [Q9UBP6-1]
CCDS8956.1. [Q9UBP6-2]
RefSeqiNP_005362.3. NM_005371.5. [Q9UBP6-1]
NP_075422.3. NM_023033.3. [Q9UBP6-2]
UniGeneiHs.42957.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKKX-ray1.55A32-265[»]
ProteinModelPortaliQ9UBP6.
SMRiQ9UBP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110392. 37 interactors.
DIPiDIP-61920N.
IntActiQ9UBP6. 1 interactor.
MINTiMINT-1440834.
STRINGi9606.ENSP00000314441.

PTM databases

iPTMnetiQ9UBP6.
PhosphoSitePlusiQ9UBP6.

Polymorphism and mutation databases

BioMutaiMETTL1.
DMDMi32171926.

Proteomic databases

EPDiQ9UBP6.
PaxDbiQ9UBP6.
PeptideAtlasiQ9UBP6.
PRIDEiQ9UBP6.

Protocols and materials databases

DNASUi4234.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257848; ENSP00000257848; ENSG00000037897. [Q9UBP6-2]
ENST00000324871; ENSP00000314441; ENSG00000037897. [Q9UBP6-1]
GeneIDi4234.
KEGGihsa:4234.
UCSCiuc009zqc.4. human. [Q9UBP6-1]

Organism-specific databases

CTDi4234.
DisGeNETi4234.
GeneCardsiMETTL1.
HGNCiHGNC:7030. METTL1.
HPAiHPA020914.
HPA050450.
MIMi604466. gene.
neXtProtiNX_Q9UBP6.
OpenTargetsiENSG00000037897.
PharmGKBiPA30766.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000169646.
HOVERGENiHBG044569.
InParanoidiQ9UBP6.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG091G0IQ7.
PhylomeDBiQ9UBP6.
TreeFamiTF314083.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciZFISH:HS00526-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.
SIGNORiQ9UBP6.

Miscellaneous databases

ChiTaRSiMETTL1. human.
EvolutionaryTraceiQ9UBP6.
GeneWikiiMETTL1.
GenomeRNAii4234.
PROiQ9UBP6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000037897.
CleanExiHS_METTL1.
ExpressionAtlasiQ9UBP6. baseline and differential.
GenevisibleiQ9UBP6. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_HUMAN
AccessioniPrimary (citable) accession number: Q9UBP6
Secondary accession number(s): B2RBX1
, H7BXF2, Q14105, Q53FS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.