Reviewed, UniProtKB/Swiss-Prot Q9UBM8 (MGT4C_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
      Short name=N-acetylglucosaminyltransferase IVc
      Short name=GlcNAc-T IVc
      Short name=GnT-IVc
    N-acetylglucosaminyltransferase IV homolog
      Short name=hGnT-IV-H

Gene names

Name:

MGAT4C

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains By similarity. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity).
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in heart, adrenal gland, testis, liver, brain and fetal brain. Not expressed in pancreas. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288596
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Signal-anchor for type II membrane protein Potential
	Topological domain	45 – 478	434	Lumenal Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	428	1	S → T: dbSNP rs17855890. Ref.1		VAR_032447

Sequences

	Sequence		Length	Mass (Da)
	Q9UBM8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7D09A4EB6B6D50C6	FASTA	478	56,047
	10 20 30 40 50 60 MFKFHQMKHI FEILDKMRCL RKRSTVSFLG VLVIFLLFMN LYIEDSYVLE GDKQLIRETS 70 80 90 100 110 120 THQLNSERYV HTFKDLSNFS GAINVTYRYL AATPLQRKRY LTIGLSSVKR KKGNYLLETI 130 140 150 160 170 180 KSIFEQSSYE ELKEISVVVH LADFNSSWRD AMVQDITQKF AHHIIAGRLM VIHAPEEYYP 190 200 210 220 230 240 ILDGLKRNYN DPEDRVKFRS KQNVDYAFLL NFCANTSDYY VMLEDDVRCS KNFLTAIKKV 250 260 270 280 290 300 IASLEGTYWV TLEFSKLGYI GKLYHSHDLP RLAHFLLMFY QEMPCDWLLT HFRGLLAQKN 310 320 330 340 350 360 VIRFKPSLFQ HMGYYSSYKG TENKLKDDDF EEESFDIPDN PPASLYTNMN VFENYEASKA 370 380 390 400 410 420 YSSVDEYFWG KPPSTGDVFV IVFENPIIIK KIKVNTGTED RQNDILHHGA LDVGENVMPS 430 440 450 460 470 KQRRQCSSYL RLGEFKNGNF EMSGVNQKIP FDIHCMRIYV TKTQKEWLII RSISIWTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the human UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV-homologue (hGnT-IV-H) gene." Furukawa T., Youssef E.M., Yatsuoka T., Yokoyama T., Makino N., Inoue H., Fukushige S., Hoshi M., Hayashi Y., Sunamura M., Horii A. J. Hum. Genet. 44:397-401(1999) [PubMed: 10570912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, VARIANT THR-428.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)-manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI." Sakamoto Y., Taguchi T., Honke K., Korekane H., Watanabe H., Tano Y., Dohmae N., Takio K., Horii A., Taniguchi N. J. Biol. Chem. 275:36029-36034(2000) [PubMed: 10962001] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB024729 mRNA. Translation: BAA83074.1. AB024730 mRNA. Translation: BAA83075.1. AB024911 Genomic DNA. Translation: BAA83087.1. BC026068 mRNA. Translation: AAH26068.1. Different termination. BC064141 mRNA. Translation: AAH64141.1.
IPI	IPI00796220.
RefSeq	NP_037376.2.
UniGene	Hs.589093
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
PTM databases
PhosphoSite	Q9UBM8.
Genome annotation databases
Ensembl	ENSG00000182050. Homo sapiens. [Contig view]
GeneID	25834.
KEGG	hsa:25834.
Organism-specific databases
GeneCards	GC12M084876.
H-InvDB	HIX0026328.
HGNC	HGNC:30871. MGAT4C.
HPA	HPA016418.
MIM	607385. gene.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9UBM8.
HOVERGEN	Q9UBM8.
Enzyme and pathway databases
BRENDA	2.4.1.145. 247.
Gene expression databases
ArrayExpress	Q9UBM8.
Bgee	Q9UBM8.
CleanEx	HS_MGAT4C.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	47137.
SOURCE	Search...

Entry information

Entry name

MGT4C_HUMAN

Accession

Primary (citable) accession number: Q9UBM8
Secondary accession number(s): Q4G199, Q9UIU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 1, 2000
Last modified:	April 14, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)