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Q9UBM8 (MGT4C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C

EC=2.4.1.145
Alternative name(s):
N-acetylglucosaminyltransferase IV homolog
Short name=hGnT-IV-H
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
Short name=GlcNAc-T IVc
Short name=GnT-IVc
Short name=N-acetylglucosaminyltransferase IVc
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
Gene names
Name:MGAT4C
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains By similarity. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity). Ref.4

Catalytic activity

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactor

Divalent metal cations By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed in heart, adrenal gland, testis, liver, brain and fetal brain. Not expressed in pancreas. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 54 family.

Sequence caution

The sequence AAH26068.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
PRO_0000288596

Regions

Topological domain1 – 2323Cytoplasmic Potential
Transmembrane24 – 4421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain45 – 478434Lumenal Potential

Amino acid modifications

Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential

Natural variations

Natural variant4281T → S. Ref.1 Ref.3
Corresponds to variant rs17855890 [ dbSNP | Ensembl ].
VAR_032447

Sequences

Sequence LengthMass (Da)Tools
Q9UBM8 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 7D09A1BB6B6800C6

FASTA47856,061
        10         20         30         40         50         60 
MFKFHQMKHI FEILDKMRCL RKRSTVSFLG VLVIFLLFMN LYIEDSYVLE GDKQLIRETS 

        70         80         90        100        110        120 
THQLNSERYV HTFKDLSNFS GAINVTYRYL AATPLQRKRY LTIGLSSVKR KKGNYLLETI 

       130        140        150        160        170        180 
KSIFEQSSYE ELKEISVVVH LADFNSSWRD AMVQDITQKF AHHIIAGRLM VIHAPEEYYP 

       190        200        210        220        230        240 
ILDGLKRNYN DPEDRVKFRS KQNVDYAFLL NFCANTSDYY VMLEDDVRCS KNFLTAIKKV 

       250        260        270        280        290        300 
IASLEGTYWV TLEFSKLGYI GKLYHSHDLP RLAHFLLMFY QEMPCDWLLT HFRGLLAQKN 

       310        320        330        340        350        360 
VIRFKPSLFQ HMGYYSSYKG TENKLKDDDF EEESFDIPDN PPASLYTNMN VFENYEASKA 

       370        380        390        400        410        420 
YSSVDEYFWG KPPSTGDVFV IVFENPIIIK KIKVNTGTED RQNDILHHGA LDVGENVMPS 

       430        440        450        460        470 
KQRRQCSTYL RLGEFKNGNF EMSGVNQKIP FDIHCMRIYV TKTQKEWLII RSISIWTS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the human UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV-homologue (hGnT-IV-H) gene."
Furukawa T., Youssef E.M., Yatsuoka T., Yokoyama T., Makino N., Inoue H., Fukushige S., Hoshi M., Hayashi Y., Sunamura M., Horii A.
J. Hum. Genet. 44:397-401(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, VARIANT SER-428.
[2]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-428.
Tissue: Testis.
[4]"Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)-manalpha 1-R[GlcNAc to man]beta 1,4N-acetylglucosaminyltransferase VI."
Sakamoto Y., Taguchi T., Honke K., Korekane H., Watanabe H., Tano Y., Dohmae N., Takio K., Horii A., Taniguchi N.
J. Biol. Chem. 275:36029-36034(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024729 mRNA. Translation: BAA83074.1.
AB024730 mRNA. Translation: BAA83075.1.
AB024911 Genomic DNA. Translation: BAA83087.1.
AC010196 Genomic DNA. No translation available.
AC016993 Genomic DNA. No translation available.
AC025157 Genomic DNA. No translation available.
AC079596 Genomic DNA. No translation available.
AC079865 Genomic DNA. No translation available.
AC087887 Genomic DNA. No translation available.
AC093122 Genomic DNA. No translation available.
AC128681 Genomic DNA. No translation available.
AC139663 Genomic DNA. No translation available.
AC139697 Genomic DNA. No translation available.
BC026068 mRNA. Translation: AAH26068.1. Sequence problems.
BC064141 mRNA. Translation: AAH64141.1.
RefSeqNP_037376.2. NM_013244.3.
XP_005268836.1. XM_005268779.2.
XP_005268838.1. XM_005268781.2.
UniGeneHs.589093.

3D structure databases

ProteinModelPortalQ9UBM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000331664.

Protein family/group databases

CAZyGT54. Glycosyltransferase Family 54.

PTM databases

PhosphoSiteQ9UBM8.

Polymorphism databases

DMDM296437368.

Proteomic databases

PaxDbQ9UBM8.
PRIDEQ9UBM8.

Protocols and materials databases

DNASU25834.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000332156; ENSP00000331664; ENSG00000182050.
ENST00000548651; ENSP00000447253; ENSG00000182050.
ENST00000549405; ENSP00000449022; ENSG00000182050.
ENST00000552808; ENSP00000446647; ENSG00000182050.
ENST00000604798; ENSP00000474896; ENSG00000182050.
GeneID25834.
KEGGhsa:25834.
UCSCuc001tah.4. human.

Organism-specific databases

CTD25834.
GeneCardsGC12M086372.
H-InvDBHIX0026328.
HGNCHGNC:30871. MGAT4C.
HPAHPA016418.
MIM607385. gene.
neXtProtNX_Q9UBM8.
PharmGKBPA143485535.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG69242.
HOGENOMHOG000070065.
HOVERGENHBG103174.
InParanoidQ9UBM8.
KOK13748.
OrthoDBEOG7F24TD.
PhylomeDBQ9UBM8.
TreeFamTF324570.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ9UBM8.
BgeeQ9UBM8.
CleanExHS_MGAT4C.
GenevestigatorQ9UBM8.

Family and domain databases

InterProIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERPTHR12062. PTHR12062. 1 hit.
PfamPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMGAT4C. human.
GenomeRNAi25834.
NextBio47137.
PROQ9UBM8.
SOURCESearch...

Entry information

Entry nameMGT4C_HUMAN
AccessionPrimary (citable) accession number: Q9UBM8
Secondary accession number(s): Q4G199, Q9UIU5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM