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Q9UBF1 (MAGC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanoma-associated antigen C2
Alternative name(s):
Cancer/testis antigen 10
Short name=CT10
Hepatocellular carcinoma-associated antigen 587
MAGE-C2 antigen
MAGE-E1 antigen
Gene names
Name:MAGEC2
Synonyms:HCA587, MAGEE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex. Ref.8

Subunit structure

Interacts with TRIM28 and UBE2H. Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleus. Note: Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma. Ref.6

Tissue specificity

Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression. Ref.3 Ref.6

Developmental stage

Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.

Sequence similarities

Contains 1 MAGE domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityPolymorphism
   Molecular functionTumor antigen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular protein catabolic process

Inferred from mutant phenotype Ref.8. Source: UniProtKB

positive regulation of ubiquitin-protein transferase activity

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.6. Source: UniProtKB

nucleus

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.8PubMed 21277283. Source: IntAct

ubiquitin protein ligase binding

Inferred from direct assay Ref.8. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Melanoma-associated antigen C2
PRO_0000156721

Regions

Domain141 – 336196MAGE
Region135 – 373239Interaction with TRIM28
Compositional bias34 – 385Poly-Glu
Compositional bias40 – 6021Ser-rich
Compositional bias67 – 704Poly-Glu
Compositional bias82 – 11837Ser-rich

Natural variations

Natural variant61G → C in a breast cancer sample; somatic mutation. Ref.10
VAR_036583

Experimental info

Mutagenesis152 – 1532LL → AA: Decreases interaction with TRIM28, abolishes in vitro ubiquitination of TP53. Ref.8
Sequence conflict121V → A in AAH13318. Ref.5
Sequence conflict1891M → T in AAF34816. Ref.1
Sequence conflict3341K → E in AAF34816. Ref.1
Sequence conflict3341K → E in AAF34817. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9UBF1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: D55867D7972BF49F

FASTA37341,163
        10         20         30         40         50         60 
MPPVPGVPFR NVDNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF SPSSFSTSSS 

        70         80         90        100        110        120 
LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL SSCCSSFSWS SFSEESSSQK 

       130        140        150        160        170        180 
GEDTGTCQGL PDSESSFTYT LDEKVAELVE FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV 

       190        200        210        220        230        240 
ILKRAREFME LLFGLALIEV GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK 

       250        260        270        280        290        300 
GNCASEEVIW EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL 

       310        320        330        340        350        360 
WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT ADDATVMASE 

       370 
SLSVMSSNVS FSE 

« Hide

References

« Hide 'large scale' references
[1]"CT10: a new cancer-testis (CT) antigen homologous to CT7 and the MAGE family, identified by representational difference analysis."
Gure A.O., Stockert E., Arden K.C., Boyer A.D., Viars C.S., Scanlan M.J., Old L.J., Chen Y.-T.
Int. J. Cancer 85:726-732(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
[2]"MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression."
Lucas S., De Plaen E., Boon T.
Int. J. Cancer 87:55-60(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
Tissue: Hepatoma.
[4]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Chronic myeloid leukemia cell and Melanoma.
[6]"HCA587 antigen expression in normal tissues and cancers: correlation with tumor differentiation in hepatocellular carcinoma."
Li B., Qian X.-P., Pang X.-W., Zou W.-Z., Wang Y.-P., Wu H.-Y., Chen W.-F.
Lab. Invest. 83:1185-1192(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines."
Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J.
Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRIM28.
[8]"MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRIM28 AND UBE2H, MUTAGENESIS OF 152-LEU-LEU-153.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF116194 mRNA. Translation: AAF34816.1.
AF116195 Genomic DNA. Translation: AAF34817.1.
AF196482 mRNA. Translation: AAF07210.1.
AF196483 Genomic DNA. Translation: AAF07211.1.
AF151378 mRNA. Translation: AAF36533.1.
AF239802 Genomic DNA. Translation: AAK15073.1.
AL031073 Genomic DNA. Translation: CAI42324.1.
BC005891 mRNA. Translation: AAH05891.1.
BC013318 mRNA. Translation: AAH13318.1.
CCDSCCDS14678.1.
RefSeqNP_057333.1. NM_016249.3.
UniGeneHs.123536.

3D structure databases

ProteinModelPortalQ9UBF1.
SMRQ9UBF1. Positions 121-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119540. 6 interactions.
IntActQ9UBF1. 4 interactions.
STRING9606.ENSP00000354660.

PTM databases

PhosphoSiteQ9UBF1.

Polymorphism databases

DMDM17380150.

Proteomic databases

MaxQBQ9UBF1.
PaxDbQ9UBF1.
PeptideAtlasQ9UBF1.
PRIDEQ9UBF1.

Protocols and materials databases

DNASU51438.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000247452; ENSP00000354660; ENSG00000046774.
GeneID51438.
KEGGhsa:51438.
UCSCuc004fbu.2. human.

Organism-specific databases

CTD51438.
GeneCardsGC0XM141291.
HGNCHGNC:13574. MAGEC2.
HPAHPA062230.
MIM300468. gene.
neXtProtNX_Q9UBF1.
PharmGKBPA134954317.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG306544.
HOGENOMHOG000231161.
HOVERGENHBG006315.
InParanoidQ9UBF1.
OMALMIVIKY.
OrthoDBEOG75F4GM.
PhylomeDBQ9UBF1.
TreeFamTF328505.

Gene expression databases

BgeeQ9UBF1.
CleanExHS_MAGEC2.
HS_MAGEE1.
GenevestigatorQ9UBF1.

Family and domain databases

InterProIPR002190. MAGE.
IPR021072. Melanoma_ass_antigen_N.
[Graphical view]
PANTHERPTHR11736. PTHR11736. 1 hit.
PfamPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMAGEC2.
GenomeRNAi51438.
NextBio55015.
PROQ9UBF1.
SOURCESearch...

Entry information

Entry nameMAGC2_HUMAN
AccessionPrimary (citable) accession number: Q9UBF1
Secondary accession number(s): Q5JZ00 expand/collapse secondary AC list , Q96D45, Q9P1M6, Q9P1M7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM