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Q9UBF1

- MAGC2_HUMAN

UniProt

Q9UBF1 - MAGC2_HUMAN

Protein

Melanoma-associated antigen C2

Gene

MAGEC2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin protein ligase binding Source: UniProtKB

    GO - Biological processi

    1. cellular protein catabolic process Source: UniProtKB
    2. positive regulation of ubiquitin-protein transferase activity Source: UniProtKB

    Keywords - Molecular functioni

    Tumor antigen

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanoma-associated antigen C2
    Alternative name(s):
    Cancer/testis antigen 10
    Short name:
    CT10
    Hepatocellular carcinoma-associated antigen 587
    MAGE-C2 antigen
    MAGE-E1 antigen
    Gene namesi
    Name:MAGEC2
    Synonyms:HCA587, MAGEE1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:13574. MAGEC2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi152 – 1532LL → AA: Decreases interaction with TRIM28, abolishes in vitro ubiquitination of TP53. 1 Publication

    Organism-specific databases

    PharmGKBiPA134954317.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Melanoma-associated antigen C2PRO_0000156721Add
    BLAST

    Proteomic databases

    MaxQBiQ9UBF1.
    PaxDbiQ9UBF1.
    PeptideAtlasiQ9UBF1.
    PRIDEiQ9UBF1.

    PTM databases

    PhosphoSiteiQ9UBF1.

    Expressioni

    Tissue specificityi

    Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression.2 Publications

    Developmental stagei

    Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.

    Gene expression databases

    BgeeiQ9UBF1.
    CleanExiHS_MAGEC2.
    HS_MAGEE1.
    GenevestigatoriQ9UBF1.

    Organism-specific databases

    HPAiHPA062230.

    Interactioni

    Subunit structurei

    Interacts with TRIM28 and UBE2H.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TP53P046373EBI-5651487,EBI-366083
    TRIM28Q1326314EBI-5651487,EBI-78139
    UBE2HP622563EBI-5651487,EBI-2129909

    Protein-protein interaction databases

    BioGridi119540. 6 interactions.
    IntActiQ9UBF1. 4 interactions.
    STRINGi9606.ENSP00000354660.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UBF1.
    SMRiQ9UBF1. Positions 121-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini141 – 336196MAGEPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni135 – 373239Interaction with TRIM28Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi34 – 385Poly-Glu
    Compositional biasi40 – 6021Ser-richAdd
    BLAST
    Compositional biasi67 – 704Poly-Glu
    Compositional biasi82 – 11837Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MAGE domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG306544.
    HOGENOMiHOG000231161.
    HOVERGENiHBG006315.
    InParanoidiQ9UBF1.
    OMAiLMIVIKY.
    OrthoDBiEOG75F4GM.
    PhylomeDBiQ9UBF1.
    TreeFamiTF328505.

    Family and domain databases

    InterProiIPR002190. MAGE.
    IPR021072. Melanoma_ass_antigen_N.
    [Graphical view]
    PANTHERiPTHR11736. PTHR11736. 1 hit.
    PfamiPF01454. MAGE. 1 hit.
    PF12440. MAGE_N. 1 hit.
    [Graphical view]
    PROSITEiPS50838. MAGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9UBF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPVPGVPFR NVDNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF    50
    SPSSFSTSSS LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL 100
    SSCCSSFSWS SFSEESSSQK GEDTGTCQGL PDSESSFTYT LDEKVAELVE 150
    FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV ILKRAREFME LLFGLALIEV 200
    GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK GNCASEEVIW 250
    EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL 300
    WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT 350
    ADDATVMASE SLSVMSSNVS FSE 373
    Length:373
    Mass (Da):41,163
    Last modified:May 1, 2000 - v1
    Checksum:iD55867D7972BF49F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121V → A in AAH13318. (PubMed:15489334)Curated
    Sequence conflicti189 – 1891M → T in AAF34816. (PubMed:10699956)Curated
    Sequence conflicti334 – 3341K → E in AAF34816. (PubMed:10699956)Curated
    Sequence conflicti334 – 3341K → E in AAF34817. (PubMed:10699956)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61G → C in a breast cancer sample; somatic mutation. 1 Publication
    VAR_036583

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF116194 mRNA. Translation: AAF34816.1.
    AF116195 Genomic DNA. Translation: AAF34817.1.
    AF196482 mRNA. Translation: AAF07210.1.
    AF196483 Genomic DNA. Translation: AAF07211.1.
    AF151378 mRNA. Translation: AAF36533.1.
    AF239802 Genomic DNA. Translation: AAK15073.1.
    AL031073 Genomic DNA. Translation: CAI42324.1.
    BC005891 mRNA. Translation: AAH05891.1.
    BC013318 mRNA. Translation: AAH13318.1.
    CCDSiCCDS14678.1.
    RefSeqiNP_057333.1. NM_016249.3.
    UniGeneiHs.123536.

    Genome annotation databases

    EnsembliENST00000247452; ENSP00000354660; ENSG00000046774.
    GeneIDi51438.
    KEGGihsa:51438.
    UCSCiuc004fbu.2. human.

    Polymorphism databases

    DMDMi17380150.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF116194 mRNA. Translation: AAF34816.1 .
    AF116195 Genomic DNA. Translation: AAF34817.1 .
    AF196482 mRNA. Translation: AAF07210.1 .
    AF196483 Genomic DNA. Translation: AAF07211.1 .
    AF151378 mRNA. Translation: AAF36533.1 .
    AF239802 Genomic DNA. Translation: AAK15073.1 .
    AL031073 Genomic DNA. Translation: CAI42324.1 .
    BC005891 mRNA. Translation: AAH05891.1 .
    BC013318 mRNA. Translation: AAH13318.1 .
    CCDSi CCDS14678.1.
    RefSeqi NP_057333.1. NM_016249.3.
    UniGenei Hs.123536.

    3D structure databases

    ProteinModelPortali Q9UBF1.
    SMRi Q9UBF1. Positions 121-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119540. 6 interactions.
    IntActi Q9UBF1. 4 interactions.
    STRINGi 9606.ENSP00000354660.

    PTM databases

    PhosphoSitei Q9UBF1.

    Polymorphism databases

    DMDMi 17380150.

    Proteomic databases

    MaxQBi Q9UBF1.
    PaxDbi Q9UBF1.
    PeptideAtlasi Q9UBF1.
    PRIDEi Q9UBF1.

    Protocols and materials databases

    DNASUi 51438.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000247452 ; ENSP00000354660 ; ENSG00000046774 .
    GeneIDi 51438.
    KEGGi hsa:51438.
    UCSCi uc004fbu.2. human.

    Organism-specific databases

    CTDi 51438.
    GeneCardsi GC0XM141291.
    HGNCi HGNC:13574. MAGEC2.
    HPAi HPA062230.
    MIMi 300468. gene.
    neXtProti NX_Q9UBF1.
    PharmGKBi PA134954317.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG306544.
    HOGENOMi HOG000231161.
    HOVERGENi HBG006315.
    InParanoidi Q9UBF1.
    OMAi LMIVIKY.
    OrthoDBi EOG75F4GM.
    PhylomeDBi Q9UBF1.
    TreeFami TF328505.

    Miscellaneous databases

    GeneWikii MAGEC2.
    GenomeRNAii 51438.
    NextBioi 55015.
    PROi Q9UBF1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9UBF1.
    CleanExi HS_MAGEC2.
    HS_MAGEE1.
    Genevestigatori Q9UBF1.

    Family and domain databases

    InterProi IPR002190. MAGE.
    IPR021072. Melanoma_ass_antigen_N.
    [Graphical view ]
    PANTHERi PTHR11736. PTHR11736. 1 hit.
    Pfami PF01454. MAGE. 1 hit.
    PF12440. MAGE_N. 1 hit.
    [Graphical view ]
    PROSITEi PS50838. MAGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CT10: a new cancer-testis (CT) antigen homologous to CT7 and the MAGE family, identified by representational difference analysis."
      Gure A.O., Stockert E., Arden K.C., Boyer A.D., Viars C.S., Scanlan M.J., Old L.J., Chen Y.-T.
      Int. J. Cancer 85:726-732(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
    2. "MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression."
      Lucas S., De Plaen E., Boon T.
      Int. J. Cancer 87:55-60(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
      Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
      J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
      Tissue: Hepatoma.
    4. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Chronic myeloid leukemia cell and Melanoma.
    6. "HCA587 antigen expression in normal tissues and cancers: correlation with tumor differentiation in hepatocellular carcinoma."
      Li B., Qian X.-P., Pang X.-W., Zou W.-Z., Wang Y.-P., Wu H.-Y., Chen W.-F.
      Lab. Invest. 83:1185-1192(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines."
      Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J.
      Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRIM28.
    8. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
      Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
      Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRIM28 AND UBE2H, MUTAGENESIS OF 152-LEU-LEU-153.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-6.

    Entry informationi

    Entry nameiMAGC2_HUMAN
    AccessioniPrimary (citable) accession number: Q9UBF1
    Secondary accession number(s): Q5JZ00
    , Q96D45, Q9P1M6, Q9P1M7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3