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Protein

Melanoma-associated antigen C2

Gene

MAGEC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.1 Publication

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • cellular protein catabolic process Source: UniProtKB
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB

Keywordsi

Molecular functionTumor antigen
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen C2
Alternative name(s):
Cancer/testis antigen 10
Short name:
CT10
Hepatocellular carcinoma-associated antigen 587
MAGE-C2 antigen
MAGE-E1 antigen
Gene namesi
Name:MAGEC2
Synonyms:HCA587, MAGEE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:13574. MAGEC2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152 – 153LL → AA: Decreases interaction with TRIM28, abolishes in vitro ubiquitination of TP53. 1 Publication2

Organism-specific databases

DisGeNETi51438.
OpenTargetsiENSG00000046774.
PharmGKBiPA134954317.

Polymorphism and mutation databases

BioMutaiMAGEC2.
DMDMi17380150.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567211 – 373Melanoma-associated antigen C2Add BLAST373

Proteomic databases

MaxQBiQ9UBF1.
PaxDbiQ9UBF1.
PeptideAtlasiQ9UBF1.
PRIDEiQ9UBF1.

PTM databases

iPTMnetiQ9UBF1.
PhosphoSitePlusiQ9UBF1.

Expressioni

Tissue specificityi

Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression.2 Publications

Developmental stagei

Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.

Gene expression databases

BgeeiENSG00000046774.
CleanExiHS_MAGEC2.
HS_MAGEE1.
GenevisibleiQ9UBF1. HS.

Organism-specific databases

HPAiHPA062230.

Interactioni

Subunit structurei

Interacts with TRIM28 and UBE2H.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119540. 23 interactors.
IntActiQ9UBF1. 8 interactors.
STRINGi9606.ENSP00000354660.

Structurei

3D structure databases

ProteinModelPortaliQ9UBF1.
SMRiQ9UBF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 336MAGEPROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 373Interaction with TRIM28Add BLAST239

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 38Poly-Glu5
Compositional biasi40 – 60Ser-richAdd BLAST21
Compositional biasi67 – 70Poly-Glu4
Compositional biasi82 – 118Ser-richAdd BLAST37

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9UBF1.
OMAiEYREVPH.
OrthoDBiEOG090A0995.
PhylomeDBiQ9UBF1.
TreeFamiTF328505.

Family and domain databases

InterProiView protein in InterPro
IPR002190. MHD_dom.
SMARTiView protein in SMART
SM01373. MAGE. 1 hit.
PROSITEiView protein in PROSITE
PS50838. MAGE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UBF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPGVPFR NVDNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF
60 70 80 90 100
SPSSFSTSSS LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL
110 120 130 140 150
SSCCSSFSWS SFSEESSSQK GEDTGTCQGL PDSESSFTYT LDEKVAELVE
160 170 180 190 200
FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV ILKRAREFME LLFGLALIEV
210 220 230 240 250
GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK GNCASEEVIW
260 270 280 290 300
EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL
310 320 330 340 350
WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT
360 370
ADDATVMASE SLSVMSSNVS FSE
Length:373
Mass (Da):41,163
Last modified:May 1, 2000 - v1
Checksum:iD55867D7972BF49F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12V → A in AAH13318 (PubMed:15489334).Curated1
Sequence conflicti189M → T in AAF34816 (PubMed:10699956).Curated1
Sequence conflicti334K → E in AAF34816 (PubMed:10699956).Curated1
Sequence conflicti334K → E in AAF34817 (PubMed:10699956).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0365836G → C in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116194 mRNA. Translation: AAF34816.1.
AF116195 Genomic DNA. Translation: AAF34817.1.
AF196482 mRNA. Translation: AAF07210.1.
AF196483 Genomic DNA. Translation: AAF07211.1.
AF151378 mRNA. Translation: AAF36533.1.
AF239802 Genomic DNA. Translation: AAK15073.1.
AL031073 Genomic DNA. Translation: CAI42324.1.
BC005891 mRNA. Translation: AAH05891.1.
BC013318 mRNA. Translation: AAH13318.1.
CCDSiCCDS14678.1.
RefSeqiNP_057333.1. NM_016249.3.
UniGeneiHs.123536.

Genome annotation databases

EnsembliENST00000247452; ENSP00000354660; ENSG00000046774.
GeneIDi51438.
KEGGihsa:51438.
UCSCiuc004fbu.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116194 mRNA. Translation: AAF34816.1.
AF116195 Genomic DNA. Translation: AAF34817.1.
AF196482 mRNA. Translation: AAF07210.1.
AF196483 Genomic DNA. Translation: AAF07211.1.
AF151378 mRNA. Translation: AAF36533.1.
AF239802 Genomic DNA. Translation: AAK15073.1.
AL031073 Genomic DNA. Translation: CAI42324.1.
BC005891 mRNA. Translation: AAH05891.1.
BC013318 mRNA. Translation: AAH13318.1.
CCDSiCCDS14678.1.
RefSeqiNP_057333.1. NM_016249.3.
UniGeneiHs.123536.

3D structure databases

ProteinModelPortaliQ9UBF1.
SMRiQ9UBF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119540. 23 interactors.
IntActiQ9UBF1. 8 interactors.
STRINGi9606.ENSP00000354660.

PTM databases

iPTMnetiQ9UBF1.
PhosphoSitePlusiQ9UBF1.

Polymorphism and mutation databases

BioMutaiMAGEC2.
DMDMi17380150.

Proteomic databases

MaxQBiQ9UBF1.
PaxDbiQ9UBF1.
PeptideAtlasiQ9UBF1.
PRIDEiQ9UBF1.

Protocols and materials databases

DNASUi51438.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247452; ENSP00000354660; ENSG00000046774.
GeneIDi51438.
KEGGihsa:51438.
UCSCiuc004fbu.3. human.

Organism-specific databases

CTDi51438.
DisGeNETi51438.
GeneCardsiMAGEC2.
HGNCiHGNC:13574. MAGEC2.
HPAiHPA062230.
MIMi300468. gene.
neXtProtiNX_Q9UBF1.
OpenTargetsiENSG00000046774.
PharmGKBiPA134954317.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9UBF1.
OMAiEYREVPH.
OrthoDBiEOG090A0995.
PhylomeDBiQ9UBF1.
TreeFamiTF328505.

Miscellaneous databases

GeneWikiiMAGEC2.
GenomeRNAii51438.
PROiQ9UBF1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000046774.
CleanExiHS_MAGEC2.
HS_MAGEE1.
GenevisibleiQ9UBF1. HS.

Family and domain databases

InterProiView protein in InterPro
IPR002190. MHD_dom.
SMARTiView protein in SMART
SM01373. MAGE. 1 hit.
PROSITEiView protein in PROSITE
PS50838. MAGE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAGC2_HUMAN
AccessioniPrimary (citable) accession number: Q9UBF1
Secondary accession number(s): Q5JZ00
, Q96D45, Q9P1M6, Q9P1M7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: February 15, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.