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Protein

Melanoma-associated antigen C2

Gene

MAGEC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.1 Publication

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • cellular protein catabolic process Source: UniProtKB
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen C2
Alternative name(s):
Cancer/testis antigen 10
Short name:
CT10
Hepatocellular carcinoma-associated antigen 587
MAGE-C2 antigen
MAGE-E1 antigen
Gene namesi
Name:MAGEC2
Synonyms:HCA587, MAGEE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:13574. MAGEC2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi152 – 1532LL → AA: Decreases interaction with TRIM28, abolishes in vitro ubiquitination of TP53. 1 Publication

Organism-specific databases

PharmGKBiPA134954317.

Polymorphism and mutation databases

BioMutaiMAGEC2.
DMDMi17380150.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Melanoma-associated antigen C2PRO_0000156721Add
BLAST

Proteomic databases

MaxQBiQ9UBF1.
PaxDbiQ9UBF1.
PeptideAtlasiQ9UBF1.
PRIDEiQ9UBF1.

PTM databases

PhosphoSiteiQ9UBF1.

Expressioni

Tissue specificityi

Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression.2 Publications

Developmental stagei

Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.

Gene expression databases

BgeeiQ9UBF1.
CleanExiHS_MAGEC2.
HS_MAGEE1.
GenevestigatoriQ9UBF1.

Organism-specific databases

HPAiHPA062230.

Interactioni

Subunit structurei

Interacts with TRIM28 and UBE2H.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P4HA3Q7Z4N83EBI-5651487,EBI-10181968
PBX3Q5JS983EBI-5651487,EBI-10244393
SSX1Q163843EBI-5651487,EBI-10237585
SSX2BQ163853EBI-5651487,EBI-2210673
TP53P046373EBI-5651487,EBI-366083
TRIM28Q1326314EBI-5651487,EBI-78139
UBE2HP622563EBI-5651487,EBI-2129909

Protein-protein interaction databases

BioGridi119540. 12 interactions.
IntActiQ9UBF1. 8 interactions.
STRINGi9606.ENSP00000354660.

Structurei

3D structure databases

ProteinModelPortaliQ9UBF1.
SMRiQ9UBF1. Positions 121-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 336196MAGEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 373239Interaction with TRIM28Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi34 – 385Poly-Glu
Compositional biasi40 – 6021Ser-richAdd
BLAST
Compositional biasi67 – 704Poly-Glu
Compositional biasi82 – 11837Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG306544.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9UBF1.
OMAiLMIVIKY.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9UBF1.
TreeFamiTF328505.

Family and domain databases

InterProiIPR021072. Melanoma_ass_antigen_N.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UBF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPGVPFR NVDNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF
60 70 80 90 100
SPSSFSTSSS LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL
110 120 130 140 150
SSCCSSFSWS SFSEESSSQK GEDTGTCQGL PDSESSFTYT LDEKVAELVE
160 170 180 190 200
FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV ILKRAREFME LLFGLALIEV
210 220 230 240 250
GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK GNCASEEVIW
260 270 280 290 300
EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL
310 320 330 340 350
WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT
360 370
ADDATVMASE SLSVMSSNVS FSE
Length:373
Mass (Da):41,163
Last modified:May 1, 2000 - v1
Checksum:iD55867D7972BF49F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121V → A in AAH13318 (PubMed:15489334).Curated
Sequence conflicti189 – 1891M → T in AAF34816 (PubMed:10699956).Curated
Sequence conflicti334 – 3341K → E in AAF34816 (PubMed:10699956).Curated
Sequence conflicti334 – 3341K → E in AAF34817 (PubMed:10699956).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 61G → C in a breast cancer sample; somatic mutation. 1 Publication
VAR_036583

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116194 mRNA. Translation: AAF34816.1.
AF116195 Genomic DNA. Translation: AAF34817.1.
AF196482 mRNA. Translation: AAF07210.1.
AF196483 Genomic DNA. Translation: AAF07211.1.
AF151378 mRNA. Translation: AAF36533.1.
AF239802 Genomic DNA. Translation: AAK15073.1.
AL031073 Genomic DNA. Translation: CAI42324.1.
BC005891 mRNA. Translation: AAH05891.1.
BC013318 mRNA. Translation: AAH13318.1.
CCDSiCCDS14678.1.
RefSeqiNP_057333.1. NM_016249.3.
UniGeneiHs.123536.

Genome annotation databases

EnsembliENST00000247452; ENSP00000354660; ENSG00000046774.
GeneIDi51438.
KEGGihsa:51438.
UCSCiuc004fbu.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116194 mRNA. Translation: AAF34816.1.
AF116195 Genomic DNA. Translation: AAF34817.1.
AF196482 mRNA. Translation: AAF07210.1.
AF196483 Genomic DNA. Translation: AAF07211.1.
AF151378 mRNA. Translation: AAF36533.1.
AF239802 Genomic DNA. Translation: AAK15073.1.
AL031073 Genomic DNA. Translation: CAI42324.1.
BC005891 mRNA. Translation: AAH05891.1.
BC013318 mRNA. Translation: AAH13318.1.
CCDSiCCDS14678.1.
RefSeqiNP_057333.1. NM_016249.3.
UniGeneiHs.123536.

3D structure databases

ProteinModelPortaliQ9UBF1.
SMRiQ9UBF1. Positions 121-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119540. 12 interactions.
IntActiQ9UBF1. 8 interactions.
STRINGi9606.ENSP00000354660.

PTM databases

PhosphoSiteiQ9UBF1.

Polymorphism and mutation databases

BioMutaiMAGEC2.
DMDMi17380150.

Proteomic databases

MaxQBiQ9UBF1.
PaxDbiQ9UBF1.
PeptideAtlasiQ9UBF1.
PRIDEiQ9UBF1.

Protocols and materials databases

DNASUi51438.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247452; ENSP00000354660; ENSG00000046774.
GeneIDi51438.
KEGGihsa:51438.
UCSCiuc004fbu.2. human.

Organism-specific databases

CTDi51438.
GeneCardsiGC0XM141291.
HGNCiHGNC:13574. MAGEC2.
HPAiHPA062230.
MIMi300468. gene.
neXtProtiNX_Q9UBF1.
PharmGKBiPA134954317.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG306544.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9UBF1.
OMAiLMIVIKY.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9UBF1.
TreeFamiTF328505.

Miscellaneous databases

GeneWikiiMAGEC2.
GenomeRNAii51438.
NextBioi55015.
PROiQ9UBF1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UBF1.
CleanExiHS_MAGEC2.
HS_MAGEE1.
GenevestigatoriQ9UBF1.

Family and domain databases

InterProiIPR021072. Melanoma_ass_antigen_N.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736. PTHR11736. 1 hit.
PfamiPF01454. MAGE. 1 hit.
PF12440. MAGE_N. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CT10: a new cancer-testis (CT) antigen homologous to CT7 and the MAGE family, identified by representational difference analysis."
    Gure A.O., Stockert E., Arden K.C., Boyer A.D., Viars C.S., Scanlan M.J., Old L.J., Chen Y.-T.
    Int. J. Cancer 85:726-732(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
  2. "MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression."
    Lucas S., De Plaen E., Boon T.
    Int. J. Cancer 87:55-60(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
    Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
    J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
    Tissue: Hepatoma.
  4. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Chronic myeloid leukemia cell and Melanoma.
  6. "HCA587 antigen expression in normal tissues and cancers: correlation with tumor differentiation in hepatocellular carcinoma."
    Li B., Qian X.-P., Pang X.-W., Zou W.-Z., Wang Y.-P., Wu H.-Y., Chen W.-F.
    Lab. Invest. 83:1185-1192(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "MAGE-A, mMage-b, and MAGE-C proteins form complexes with KAP1 and suppress p53-dependent apoptosis in MAGE-positive cell lines."
    Yang B., O'Herrin S.M., Wu J., Reagan-Shaw S., Ma Y., Bhat K.M., Gravekamp C., Setaluri V., Peters N., Hoffmann F.M., Peng H., Ivanov A.V., Simpson A.J., Longley B.J.
    Cancer Res. 67:9954-9962(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM28.
  8. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
    Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
    Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRIM28 AND UBE2H, MUTAGENESIS OF 152-LEU-LEU-153.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-6.

Entry informationi

Entry nameiMAGC2_HUMAN
AccessioniPrimary (citable) accession number: Q9UBF1
Secondary accession number(s): Q5JZ00
, Q96D45, Q9P1M6, Q9P1M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.