Q9UBF1 (MAGC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanoma-associated antigen C2

Alternative name(s):
Cancer/testis antigen 10
Short name=CT10
Hepatocellular carcinoma-associated antigen 587
MAGE-C2 antigen
MAGE-E1 antigen

Gene names

Name:	MAGEC2
Synonyms:	HCA587, MAGEE1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm. Nucleus. Note: Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma. Ref.6
Tissue specificity	Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression. Ref.3 Ref.6
Developmental stage	Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.
Sequence similarities	Contains 1 MAGE domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Tumor antigen
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay Ref.6. Source: UniProtKB nucleus Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Melanoma-associated antigen C2		PRO_0000156721
Regions
	Domain	141 – 336	196	MAGE
	Compositional bias	34 – 38	5	Poly-Glu
	Compositional bias	40 – 60	21	Ser-rich
	Compositional bias	67 – 70	4	Poly-Glu
	Compositional bias	82 – 118	37	Ser-rich
Amino acid modifications
	Modified residue	259	1	Phosphotyrosine Ref.7
Natural variations
	Natural variant	6	1	G → C in a breast cancer sample; somatic mutation. Ref.9		VAR_036583
Experimental info
	Sequence conflict	12	1	V → A in AAH13318. Ref.5
	Sequence conflict	189	1	M → T in AAF34816. Ref.1
	Sequence conflict	334	1	K → E in AAF34816. Ref.1
	Sequence conflict	334	1	K → E in AAF34817. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9UBF1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D55867D7972BF49F	FASTA	373	41,163
	10 20 30 40 50 60 MPPVPGVPFR NVDNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF SPSSFSTSSS 70 80 90 100 110 120 LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL SSCCSSFSWS SFSEESSSQK 130 140 150 160 170 180 GEDTGTCQGL PDSESSFTYT LDEKVAELVE FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV 190 200 210 220 230 240 ILKRAREFME LLFGLALIEV GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK 250 260 270 280 290 300 GNCASEEVIW EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL 310 320 330 340 350 360 WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT ADDATVMASE 370 SLSVMSSNVS FSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CT10: a new cancer-testis (CT) antigen homologous to CT7 and the MAGE family, identified by representational difference analysis." Gure A.O., Stockert E., Arden K.C., Boyer A.D., Viars C.S., Scanlan M.J., Old L.J., Chen Y.-T. Int. J. Cancer 85:726-732(2000) [PubMed: 10699956] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
[2]	"MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression." Lucas S., De Plaen E., Boon T. Int. J. Cancer 87:55-60(2000) [PubMed: 10861452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies." Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F. J. Immunol. 169:1102-1109(2002) [PubMed: 12097419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN. Tissue: Hepatoma.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Chronic myeloid leukemia cell and Melanoma.
[6]	"HCA587 antigen expression in normal tissues and cancers: correlation with tumor differentiation in hepatocellular carcinoma." Li B., Qian X.-P., Pang X.-W., Zou W.-Z., Wang Y.-P., Wu H.-Y., Chen W.-F. Lab. Invest. 83:1185-1192(2003) [PubMed: 12920247] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-259, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF116194 mRNA. Translation: AAF34816.1. AF116195 Genomic DNA. Translation: AAF34817.1. AF196482 mRNA. Translation: AAF07210.1. AF196483 Genomic DNA. Translation: AAF07211.1. AF151378 mRNA. Translation: AAF36533.1. AF239802 Genomic DNA. Translation: AAK15073.1. AL031073 Genomic DNA. Translation: CAI42324.1. BC005891 mRNA. Translation: AAH05891.1. BC013318 mRNA. Translation: AAH13318.1.
IPI	IPI00008278.
RefSeq	NP_057333.1. NM_016249.3.
UniGene	Hs.123536.
3D structure databases
ProteinModelPortal	Q9UBF1.
SMR	Q9UBF1. Positions 125-339.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UBF1.
PTM databases
PhosphoSite	Q9UBF1.
Polymorphism databases
DMDM	17380150.
Proteomic databases
PeptideAtlas	Q9UBF1.
PRIDE	Q9UBF1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000247452; ENSP00000354660; ENSG00000046774.
GeneID	51438.
KEGG	hsa:51438.
UCSC	uc004fbu.1. human.
Organism-specific databases
CTD	51438.
GeneCards	GC0XM141291.
H-InvDB	HIX0017094.
HGNC	HGNC:13574. MAGEC2.
MIM	300468. gene.
neXtProt	NX_Q9UBF1.
PharmGKB	PA134954317.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13488.
GeneTree	ENSGT00560000076862.
HOVERGEN	HBG006315.
InParanoid	Q9UBF1.
OMA	EAEMLMI.
OrthoDB	EOG43R3N7.
PhylomeDB	Q9UBF1.
Gene expression databases
ArrayExpress	Q9UBF1.
Bgee	Q9UBF1.
CleanEx	HS_MAGEC2. HS_MAGEE1.
Genevestigator	Q9UBF1.
GermOnline	ENSG00000046774. Homo sapiens.
Family and domain databases
InterPro	IPR002190. MAGE. IPR021072. Melanoma_ass_antigen_N. [Graphical view]
PANTHER	PTHR11736. MAGE. 1 hit.
Pfam	PF01454. MAGE. 1 hit. PF12440. MAGE_N. 1 hit. [Graphical view]
PROSITE	PS50838. MAGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	55015.
SOURCE	Search...

Entry information

Entry name

MAGC2_HUMAN

Accession

Primary (citable) accession number: Q9UBF1
Secondary accession number(s): Q5JZ00 , Q96D45, Q9P1M6, Q9P1M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.