Q9UBD5 (ORC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Origin recognition complex subunit 3

Alternative name(s):
Origin recognition complex subunit Latheo

Gene names

Name:	ORC3
Synonyms:	LATHEO, ORC3L

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Subunit structure	ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.12 Ref.13 Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.12 Ref.13
Sequence similarities	Belongs to the ORC3 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Traceable author statement. Source: Reactome G1/S transition of mitotic cell cycle Traceable author statement. Source: Reactome M/G1 transition of mitotic cell cycle Traceable author statement. Source: Reactome S phase of mitotic cell cycle Traceable author statement. Source: Reactome cell cycle checkpoint Traceable author statement. Source: Reactome
Cellular component	nuclear origin of replication recognition complex Inferred from direct assay. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Molecular function	DNA replication origin binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UBD5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UBD5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     505-505: D → DA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 711	711	Origin recognition complex subunit 3		PRO_0000127083
Amino acid modifications
	Modified residue	23	1	Phosphoserine Ref.13
	Modified residue	208	1	Phosphoserine Ref.12
	Modified residue	516	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	505	1	D → DA in isoform 2.		VSP_017129
	Natural variant	94	1	Q → K. Ref.6 Corresponds to variant rs2307365 [ dbSNP | Ensembl ].		VAR_014516
	Natural variant	126	1	Q → R. Ref.6 Corresponds to variant rs2307371 [ dbSNP | Ensembl ].		VAR_014517
	Natural variant	217	1	V → I. Ref.6 Corresponds to variant rs2307389 [ dbSNP | Ensembl ].		VAR_014518
	Natural variant	247	1	I → V. Ref.6 Corresponds to variant rs2307374 [ dbSNP | Ensembl ].		VAR_014519
	Natural variant	287	1	T → M. Corresponds to variant rs2307381 [ dbSNP | Ensembl ].		VAR_014520
	Natural variant	389	1	T → P. Corresponds to variant rs2307372 [ dbSNP | Ensembl ].		VAR_014521
	Natural variant	588	1	R → C. Corresponds to variant rs2307370 [ dbSNP | Ensembl ].		VAR_014522
	Natural variant	626	1	A → T. Ref.6 Corresponds to variant rs28381545 [ dbSNP | Ensembl ].		VAR_020656
Experimental info
	Sequence conflict	56	1	S → F in BAD96515. Ref.5
	Sequence conflict	86	1	K → R in CAB45715. Ref.4
	Sequence conflict	137	1	Q → R in CAB45715. Ref.4
	Sequence conflict	471	1	I → M in BAD96515. Ref.5
	Sequence conflict	531	1	K → R in CAB45715. Ref.4
	Sequence conflict	539	1	L → F in AAD40220. Ref.3
	Sequence conflict	539	1	L → F in AAA96313. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E713569A11B39B1C	FASTA	711	82,254
	10 20 30 40 50 60 MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER 70 80 90 100 110 120 LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS 130 140 150 160 170 180 LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS 190 200 210 220 230 240 MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH 250 260 270 280 290 300 LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF 310 320 330 340 350 360 KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN 370 380 390 400 410 420 NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH 430 440 450 460 470 480 KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK 490 500 510 520 530 540 SYCENHLGST AKRIEEFLAQ FQSLDETKEE EDASGSQPKG LQKTDLYHLQ KSLLEMKELR 550 560 570 580 590 600 RSKKQTKFEV LRENVVNFID CLVREYLLPP ETQPLHEVVY FSAAHALREH LNAAPRIALH 610 620 630 640 650 660 TALNNPYYYL KNEALKSEEG CIPNIAPDIC IAYKLHLECS RLINLVDWSE AFATVVTAAE 670 680 690 700 710 KMDANSATSE EMNEIIHARF IRAVSELELL GFIKPTKQKT DHVARLTWGG C
	Isoform 2 [UniParc]. Checksum: 2F7953FCB84DD63F	FASTA	712	82,325
	10 20 30 40 50 60 MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER 70 80 90 100 110 120 LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS 130 140 150 160 170 180 LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS 190 200 210 220 230 240 MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH 250 260 270 280 290 300 LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF 310 320 330 340 350 360 KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN 370 380 390 400 410 420 NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH 430 440 450 460 470 480 KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK 490 500 510 520 530 540 SYCENHLGST AKRIEEFLAQ FQSLDAETKE EEDASGSQPK GLQKTDLYHL QKSLLEMKEL 550 560 570 580 590 600 RRSKKQTKFE VLRENVVNFI DCLVREYLLP PETQPLHEVV YFSAAHALRE HLNAAPRIAL 610 620 630 640 650 660 HTALNNPYYY LKNEALKSEE GCIPNIAPDI CIAYKLHLEC SRLINLVDWS EAFATVVTAA 670 680 690 700 710 EKMDANSATS EEMNEIIHAR FIRAVSELEL LGFIKPTKQK TDHVARLTWG GC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"cDNA cloning of a homolog for Saccharomyces cerevisiae ORC3 from Homo sapiens." Dean F.B., O'Donnell M. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant." Pinto S., Quintana D.G., Smith P., Mihalek R.M., Hou Z.-H., Boynton S., Jones C.J., Handricks M., Velinzon K., Wohlschlegel J.A., Austin R.J., Lane W.S., Dutta A., Tully T. Neuron 23:45-54(1999) [PubMed: 10402192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[6]	NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-94; ARG-126; ILE-217; VAL-247 AND THR-626.
[7]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-671 (ISOFORM 2). Tissue: Testis and Uterus.
[9]	Mihalek R., Homanics G.E. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-542 (ISOFORM 1). Tissue: Brain.
[10]	"The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle." Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C. J. Biol. Chem. 278:41535-41540(2003) [PubMed: 12909626] [Abstract] Cited for: IDENTIFICATION IN THE ORC COMPLEX, MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX.
[11]	"ATP-dependent assembly of the human origin recognition complex." Siddiqui K., Stillman B. J. Biol. Chem. 282:32370-32383(2007) [PubMed: 17716973] [Abstract] Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX.
[12]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208 AND SER-516, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135044 mRNA. Translation: AAD30282.1. AF125507 mRNA. Translation: AAD18057.1. AF093535 mRNA. Translation: AAD40220.1. AL080116 mRNA. Translation: CAB45715.1. AK222795 mRNA. Translation: BAD96515.1. AY623113 Genomic DNA. Translation: AAT38109.1. AL451126, AL133211 Genomic DNA. Translation: CAI10900.1. AL133211, AL451126 Genomic DNA. Translation: CAI21539.1. AL451126, AL133211 Genomic DNA. Translation: CAM13063.1. AL133211, AL451126 Genomic DNA. Translation: CAM28255.1. BC035494 mRNA. Translation: AAH35494.1. BC047689 mRNA. Translation: AAH47689.1. Different termination. U50950 mRNA. Translation: AAA96313.1.
IPI	IPI00294402. IPI00374747.
PIR	T12452.
RefSeq	NP_036513.2. NM_012381.3. NP_862820.1. NM_181837.2.
UniGene	Hs.410228.
3D structure databases
ProteinModelPortal	Q9UBD5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UBD5. 14 interactions.
MINT	MINT-1201384.
STRING	Q9UBD5.
PTM databases
PhosphoSite	Q9UBD5.
Polymorphism databases
DMDM	8928268.
Proteomic databases
PRIDE	Q9UBD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000392844; ENSP00000376586; ENSG00000135336.
GeneID	23595.
KEGG	hsa:23595.
UCSC	uc003pmg.1. human. uc003pmh.1. human.
Organism-specific databases
CTD	23595.
GeneCards	GC06P088299.
H-InvDB	HIX0006056.
HGNC	HGNC:8489. ORC3.
MIM	604972. gene.
neXtProt	NX_Q9UBD5.
PharmGKB	PA32810.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07201.
GeneTree	ENSGT00390000011376.
HOVERGEN	HBG007599.
OMA	SPIIIHR.
OrthoDB	EOG4X97GR.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	Q9UBD5.
Bgee	Q9UBD5.
Genevestigator	Q9UBD5.
GermOnline	ENSG00000135336. Homo sapiens.
Family and domain databases
InterPro	IPR020795. ORC3. [Graphical view]
KO	K02605.
PANTHER	PTHR12748. ORC3. 1 hit.
Pfam	PF07034. ORC3_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	46252.
SOURCE	Search...

Entry information

Entry name

ORC3_HUMAN

Accession

Primary (citable) accession number: Q9UBD5
Secondary accession number(s): A2A2T5 , Q13565, Q53GY6, Q5T159, Q6IUY7, Q86TN5, Q9UG44, Q9UNT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.