Reviewed, UniProtKB/Swiss-Prot Q9UBD5 (ORC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Origin recognition complex subunit 3
Alternative name(s):
    Origin recognition complex subunit Latheo

Gene names

Name:	ORC3L
Synonyms:	LATHEO, ORC3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the origin recognition complex (ORC) that binds origins of replication. Binds to the ARS consensus sequence (ACS) of origins of replication in an ATP-dependent manner.
Subunit structure	ORC is composed of six subunits By similarity.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.10 Ref.11 Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.11
Sequence similarities	Belongs to the ORC3 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Ref.3 Inferred from Experiment. Source: Reactome
Cellular component	nuclear origin of replication recognition complex Inferred from electronic annotation. Source: InterPro nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	DNA replication origin binding Traceable author statement. Source: ProtInc protein binding Ref.10 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRCA1	P38398	1	EBI-374916,EBI-349905
CCNE2	O96020	1	EBI-374916,EBI-375033
CDC45L	O75419	1	EBI-374916,EBI-374969
CDC6	Q99741	1	EBI-374916,EBI-374862
MCM10	Q7L590	1	EBI-374916,EBI-374912
MCM5	P33992	1	EBI-374916,EBI-359410
MCM7	P33993	1	EBI-374916,EBI-355924
ORC1L	Q13415	1	EBI-374916,EBI-374847
ORC4L	O43929	1	EBI-374916,EBI-374889
ORC5L	O43913	1	EBI-374916,EBI-374928
ORC6L	Q9Y5N6	1	EBI-374916,EBI-374840

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UBD5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UBD5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     505-505: D → DA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 711	711	Origin recognition complex subunit 3		PRO_0000127083
Amino acid modifications
	Modified residue	23	1	Phosphoserine Ref.11
	Modified residue	208	1	Phosphoserine Ref.10
	Modified residue	516	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	505	1	D → DA in isoform 2.		VSP_017129
	Natural variant	94	1	Q → K: dbSNP rs2307365. Ref.6		VAR_014516
	Natural variant	126	1	Q → R: dbSNP rs2307371. Ref.6		VAR_014517
	Natural variant	217	1	V → I: dbSNP rs2307389. Ref.6		VAR_014518
	Natural variant	247	1	I → V: dbSNP rs2307374. Ref.6		VAR_014519
	Natural variant	287	1	T → M: dbSNP rs2307381.		VAR_014520
	Natural variant	389	1	T → P: dbSNP rs2307372.		VAR_014521
	Natural variant	588	1	R → C: dbSNP rs2307370.		VAR_014522
	Natural variant	626	1	A → T: dbSNP rs28381545. Ref.6		VAR_020656
Experimental info
	Sequence conflict	56	1	S → F in BAD96515. Ref.5
	Sequence conflict	86	1	K → R in CAB45715. Ref.4
	Sequence conflict	137	1	Q → R in CAB45715. Ref.4
	Sequence conflict	471	1	I → M in BAD96515. Ref.5
	Sequence conflict	531	1	K → R in CAB45715. Ref.4
	Sequence conflict	539	1	L → F in AAD40220. Ref.3
	Sequence conflict	539	1	L → F in AAA96313. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E713569A11B39B1C	FASTA	711	82,254
	10 20 30 40 50 60 MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER 70 80 90 100 110 120 LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS 130 140 150 160 170 180 LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS 190 200 210 220 230 240 MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH 250 260 270 280 290 300 LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF 310 320 330 340 350 360 KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN 370 380 390 400 410 420 NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH 430 440 450 460 470 480 KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK 490 500 510 520 530 540 SYCENHLGST AKRIEEFLAQ FQSLDETKEE EDASGSQPKG LQKTDLYHLQ KSLLEMKELR 550 560 570 580 590 600 RSKKQTKFEV LRENVVNFID CLVREYLLPP ETQPLHEVVY FSAAHALREH LNAAPRIALH 610 620 630 640 650 660 TALNNPYYYL KNEALKSEEG CIPNIAPDIC IAYKLHLECS RLINLVDWSE AFATVVTAAE 670 680 690 700 710 KMDANSATSE EMNEIIHARF IRAVSELELL GFIKPTKQKT DHVARLTWGG C
	Isoform 2. Checksum: 2F7953FCB84DD63F	FASTA	712	82,325
	10 20 30 40 50 60 MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER 70 80 90 100 110 120 LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS 130 140 150 160 170 180 LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS 190 200 210 220 230 240 MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH 250 260 270 280 290 300 LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF 310 320 330 340 350 360 KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN 370 380 390 400 410 420 NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH 430 440 450 460 470 480 KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK 490 500 510 520 530 540 SYCENHLGST AKRIEEFLAQ FQSLDAETKE EEDASGSQPK GLQKTDLYHL QKSLLEMKEL 550 560 570 580 590 600 RRSKKQTKFE VLRENVVNFI DCLVREYLLP PETQPLHEVV YFSAAHALRE HLNAAPRIAL 610 620 630 640 650 660 HTALNNPYYY LKNEALKSEE GCIPNIAPDI CIAYKLHLEC SRLINLVDWS EAFATVVTAA 670 680 690 700 710 EKMDANSATS EEMNEIIHAR FIRAVSELEL LGFIKPTKQK TDHVARLTWG GC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"cDNA cloning of a homolog for Saccharomyces cerevisiae ORC3 from Homo sapiens." Dean F.B., O'Donnell M. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant." Pinto S., Quintana D.G., Smith P., Mihalek R.M., Hou Z.-H., Boynton S., Jones C.J., Handricks M., Velinzon K., Wohlschlegel J.A., Austin R.J., Lane W.S., Dutta A., Tully T. Neuron 23:45-54(1999) [PubMed: 10402192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[6]	NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-94; ARG-126; ILE-217; VAL-247 AND THR-626.
[7]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-671 (ISOFORM 2). Tissue: Testis and Uterus.
[9]	Mihalek R., Homanics G.E. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-542 (ISOFORM 1). Tissue: Brain.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208 AND SER-516, MASS SPECTROMETRY.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, MASS SPECTROMETRY.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135044 mRNA. Translation: AAD30282.1. AF125507 mRNA. Translation: AAD18057.1. AF093535 mRNA. Translation: AAD40220.1. AL080116 mRNA. Translation: CAB45715.1. AK222795 mRNA. Translation: BAD96515.1. AY623113 Genomic DNA. Translation: AAT38109.1. AL451126, AL133211 Genomic DNA. Translation: CAI10900.1. AL133211, AL451126 Genomic DNA. Translation: CAI21539.1. AL451126, AL133211 Genomic DNA. Translation: CAM13063.1. AL133211, AL451126 Genomic DNA. Translation: CAM28255.1. BC035494 mRNA. Translation: AAH35494.1. BC047689 mRNA. Translation: AAH47689.1. Different termination. U50950 mRNA. Translation: AAA96313.1.
IPI	IPI00294402. IPI00374747.
PIR	T12452.
RefSeq	NP_036513.2. NP_862820.1.
UniGene	Hs.410228
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UBD5. 11 interactions.
STRING	Q9UBD5.
PTM databases
PhosphoSite	Q9UBD5.
Proteomic databases
PRIDE	Q9UBD5.
Genome annotation databases
Ensembl	ENST00000392844; ENSP00000376586; ENSG00000135336; Homo sapiens. [Genome view]
GeneID	23595.
KEGG	hsa:23595.
UCSC	uc003pmg.1. human. uc003pmh.1. human.
Organism-specific databases
CTD	23595.
GeneCards	GC06P088356.
H-InvDB	HIX0006056.
HGNC	HGNC:8489. ORC3L.
MIM	604972. gene.
PharmGKB	PA32810.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07201.
HOVERGEN	Q9UBD5.
OMA	SPIIIHR.
PhylomeDB	Q9UBD5.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	Q9UBD5.
Bgee	Q9UBD5.
Genevestigator	Q9UBD5.
GermOnline	ENSG00000135336. Homo sapiens.
Family and domain databases
InterPro	IPR020795. ORC3. [Graphical view]
PANTHER	PTHR12748. ORC3. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	46252.
SOURCE	Search...

Entry information

Entry name

ORC3_HUMAN

Accession

Primary (citable) accession number: Q9UBD5
Secondary accession number(s): A2A2T5 , Q13565, Q53GY6, Q5T159, Q6IUY7, Q86TN5, Q9UG44, Q9UNT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.