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Q9UBD5 (ORC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Origin recognition complex subunit 3
Alternative name(s):
Origin recognition complex subunit Latheo
Gene names
Name:ORC3
Synonyms:LATHEO, ORC3L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3. Ref.16

Subunit structure

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Ref.11

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ORC3 family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UBD5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UBD5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     505-505: D → DA
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9UBD5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Origin recognition complex subunit 3
PRO_0000127083

Amino acid modifications

Modified residue231Phosphoserine Ref.14
Modified residue5161Phosphoserine Ref.13

Natural variations

Alternative sequence1 – 143143Missing in isoform 3.
VSP_044893
Alternative sequence5051D → DA in isoform 2.
VSP_017129
Natural variant941Q → K. Ref.7
Corresponds to variant rs2307365 [ dbSNP | Ensembl ].
VAR_014516
Natural variant1261Q → R. Ref.7
Corresponds to variant rs2307371 [ dbSNP | Ensembl ].
VAR_014517
Natural variant2171V → I. Ref.7
Corresponds to variant rs2307389 [ dbSNP | Ensembl ].
VAR_014518
Natural variant2471I → V. Ref.7
Corresponds to variant rs2307374 [ dbSNP | Ensembl ].
VAR_014519
Natural variant2871T → M.
Corresponds to variant rs2307381 [ dbSNP | Ensembl ].
VAR_014520
Natural variant3891T → P.
Corresponds to variant rs2307372 [ dbSNP | Ensembl ].
VAR_014521
Natural variant5881R → C.
Corresponds to variant rs2307370 [ dbSNP | Ensembl ].
VAR_014522
Natural variant6261A → T. Ref.7
Corresponds to variant rs28381545 [ dbSNP | Ensembl ].
VAR_020656

Experimental info

Sequence conflict561S → F in BAD96515. Ref.5
Sequence conflict861K → R in CAB45715. Ref.4
Sequence conflict1371Q → R in CAB45715. Ref.4
Sequence conflict4711I → M in BAD96515. Ref.5
Sequence conflict5311K → R in CAB45715. Ref.4
Sequence conflict5391L → F in AAD40220. Ref.3
Sequence conflict5391L → F in AAA96313. Ref.10

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: E713569A11B39B1C

FASTA71182,254
        10         20         30         40         50         60 
MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER 

        70         80         90        100        110        120 
LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS 

       130        140        150        160        170        180 
LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS 

       190        200        210        220        230        240 
MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH 

       250        260        270        280        290        300 
LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF 

       310        320        330        340        350        360 
KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN 

       370        380        390        400        410        420 
NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH 

       430        440        450        460        470        480 
KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK 

       490        500        510        520        530        540 
SYCENHLGST AKRIEEFLAQ FQSLDETKEE EDASGSQPKG LQKTDLYHLQ KSLLEMKELR 

       550        560        570        580        590        600 
RSKKQTKFEV LRENVVNFID CLVREYLLPP ETQPLHEVVY FSAAHALREH LNAAPRIALH 

       610        620        630        640        650        660 
TALNNPYYYL KNEALKSEEG CIPNIAPDIC IAYKLHLECS RLINLVDWSE AFATVVTAAE 

       670        680        690        700        710 
KMDANSATSE EMNEIIHARF IRAVSELELL GFIKPTKQKT DHVARLTWGG C 

« Hide

Isoform 2 [UniParc].

Checksum: 2F7953FCB84DD63F
Show »

FASTA71282,325
Isoform 3 [UniParc].

Checksum: 6FEEBF7290C82A97
Show »

FASTA56865,929

References

« Hide 'large scale' references
[1]Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"cDNA cloning of a homolog for Saccharomyces cerevisiae ORC3 from Homo sapiens."
Dean F.B., O'Donnell M.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant."
Pinto S., Quintana D.G., Smith P., Mihalek R.M., Hou Z.-H., Boynton S., Jones C.J., Handricks M., Velinzon K., Wohlschlegel J.A., Austin R.J., Lane W.S., Dutta A., Tully T.
Neuron 23:45-54(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[5]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Liver.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Thymus.
[7]NIEHS SNPs program
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-94; ARG-126; ILE-217; VAL-247 AND THR-626.
[8]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-671 (ISOFORM 2).
Tissue: Testis and Uterus.
[10]Mihalek R., Homanics G.E.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-542 (ISOFORM 1).
Tissue: Brain.
[11]"The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle."
Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C.
J. Biol. Chem. 278:41535-41540(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ORC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX.
[12]"ATP-dependent assembly of the human origin recognition complex."
Siddiqui K., Stillman B.
J. Biol. Chem. 282:32370-32383(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX.
[13]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing."
Chan K.M., Zhang Z.
J. Biol. Chem. 287:15024-15033(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BINDING TO HISTONE H3 AND H4 TRIMETHYLATION MARKS.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF135044 mRNA. Translation: AAD30282.1.
AF125507 mRNA. Translation: AAD18057.1.
AF093535 mRNA. Translation: AAD40220.1.
AL080116 mRNA. Translation: CAB45715.1.
AK222795 mRNA. Translation: BAD96515.1.
AK303195 mRNA. Translation: BAG64287.1.
AK315911 mRNA. Translation: BAH14282.1.
AY623113 Genomic DNA. Translation: AAT38109.1.
AL451126, AL133211 Genomic DNA. Translation: CAI10900.1.
AL133211, AL451126 Genomic DNA. Translation: CAI21539.1.
AL451126, AL133211 Genomic DNA. Translation: CAM13063.1.
AL133211, AL451126 Genomic DNA. Translation: CAM28255.1.
BC035494 mRNA. Translation: AAH35494.1.
BC047689 mRNA. Translation: AAH47689.1. Different termination.
U50950 mRNA. Translation: AAA96313.1.
PIRT12452.
RefSeqNP_001184188.1. NM_001197259.1.
NP_036513.2. NM_012381.3.
NP_862820.1. NM_181837.2.
UniGeneHs.410228.

3D structure databases

ProteinModelPortalQ9UBD5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117130. 21 interactions.
DIPDIP-31735N.
IntActQ9UBD5. 20 interactions.
MINTMINT-1201384.

PTM databases

PhosphoSiteQ9UBD5.

Polymorphism databases

DMDM8928268.

Proteomic databases

PaxDbQ9UBD5.
PRIDEQ9UBD5.

Protocols and materials databases

DNASU23595.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000257789; ENSP00000257789; ENSG00000135336. [Q9UBD5-2]
ENST00000392844; ENSP00000376586; ENSG00000135336. [Q9UBD5-1]
ENST00000546266; ENSP00000444695; ENSG00000135336. [Q9UBD5-3]
GeneID23595.
KEGGhsa:23595.
UCSCuc003pmg.3. human. [Q9UBD5-2]
uc003pmh.3. human. [Q9UBD5-1]

Organism-specific databases

CTD23595.
GeneCardsGC06P088299.
HGNCHGNC:8489. ORC3.
HPAHPA053748.
MIM604972. gene.
neXtProtNX_Q9UBD5.
PharmGKBPA32810.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG296366.
HOVERGENHBG007599.
KOK02605.
OMACFVFKPN.
OrthoDBEOG78PV8B.
PhylomeDBQ9UBD5.
TreeFamTF101093.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.
REACT_383. DNA Replication.

Gene expression databases

ArrayExpressQ9UBD5.
BgeeQ9UBD5.
GenevestigatorQ9UBD5.

Family and domain databases

InterProIPR020795. ORC3.
[Graphical view]
PANTHERPTHR12748. PTHR12748. 1 hit.
PfamPF07034. ORC3_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSORC3. human.
GeneWikiORC3.
ORC3L.
GenomeRNAi23595.
NextBio46252.
PROQ9UBD5.
SOURCESearch...

Entry information

Entry nameORC3_HUMAN
AccessionPrimary (citable) accession number: Q9UBD5
Secondary accession number(s): A2A2T5 expand/collapse secondary AC list , B4E025, Q13565, Q53GY6, Q5T159, Q6IUY7, Q86TN5, Q9UG44, Q9UNT6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM