Q9UBD5 (ORC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 98.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Origin recognition complex subunit 3 Alternative name(s): Origin recognition complex subunit Latheo | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 711 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. |
| Subunit structure | ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. |
| Subcellular location | |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.12 Ref.13 Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.12 Ref.13 |
| Sequence similarities | Belongs to the ORC3 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Ligand | DNA-binding |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | DNA replication Traceable author statement. Source: Reactome G1/S transition of mitotic cell cycleTraceable author statement. Source: Reactome M/G1 transition of mitotic cell cycleTraceable author statement. Source: Reactome S phase of mitotic cell cycleTraceable author statement. Source: Reactome cell cycle checkpointTraceable author statement. Source: Reactome |
| Cellular component | nuclear origin of replication recognition complex Inferred from direct assay. Source: UniProtKB nucleoplasmTraceable author statement. Source: Reactome |
| Molecular function | DNA replication origin binding Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9UBD5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9UBD5-2) The sequence of this isoform differs from the canonical sequence as follows: 505-505: D → DA | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 711 | 711 | Origin recognition complex subunit 3 | PRO_0000127083 | |||||
Amino acid modifications | |||||||||
| Modified residue | 23 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 208 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 516 | 1 | Phosphoserine Ref.12 | ||||||
Natural variations | |||||||||
| Alternative sequence | 505 | 1 | D → DA in isoform 2. | VSP_017129 | |||||
| Natural variant | 94 | 1 | Q → K. Ref.6 Corresponds to variant rs2307365 [ dbSNP | Ensembl ]. | VAR_014516 | |||||
| Natural variant | 126 | 1 | Q → R. Ref.6 Corresponds to variant rs2307371 [ dbSNP | Ensembl ]. | VAR_014517 | |||||
| Natural variant | 217 | 1 | V → I. Ref.6 Corresponds to variant rs2307389 [ dbSNP | Ensembl ]. | VAR_014518 | |||||
| Natural variant | 247 | 1 | I → V. Ref.6 Corresponds to variant rs2307374 [ dbSNP | Ensembl ]. | VAR_014519 | |||||
| Natural variant | 287 | 1 | T → M. Corresponds to variant rs2307381 [ dbSNP | Ensembl ]. | VAR_014520 | |||||
| Natural variant | 389 | 1 | T → P. Corresponds to variant rs2307372 [ dbSNP | Ensembl ]. | VAR_014521 | |||||
| Natural variant | 588 | 1 | R → C. Corresponds to variant rs2307370 [ dbSNP | Ensembl ]. | VAR_014522 | |||||
| Natural variant | 626 | 1 | A → T. Ref.6 Corresponds to variant rs28381545 [ dbSNP | Ensembl ]. | VAR_020656 | |||||
Experimental info | |||||||||
| Sequence conflict | 56 | 1 | S → F in BAD96515. Ref.5 | ||||||
| Sequence conflict | 86 | 1 | K → R in CAB45715. Ref.4 | ||||||
| Sequence conflict | 137 | 1 | Q → R in CAB45715. Ref.4 | ||||||
| Sequence conflict | 471 | 1 | I → M in BAD96515. Ref.5 | ||||||
| Sequence conflict | 531 | 1 | K → R in CAB45715. Ref.4 | ||||||
| Sequence conflict | 539 | 1 | L → F in AAD40220. Ref.3 | ||||||
| Sequence conflict | 539 | 1 | L → F in AAA96313. Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "cDNA cloning of a homolog for Saccharomyces cerevisiae ORC3 from Homo sapiens." Dean F.B., O'Donnell M. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [3] | "Latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant." Pinto S., Quintana D.G., Smith P., Mihalek R.M., Hou Z.-H., Boynton S., Jones C.J., Handricks M., Velinzon K., Wohlschlegel J.A., Austin R.J., Lane W.S., Dutta A., Tully T. Neuron 23:45-54(1999) [PubMed: 10402192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [4] | "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain. |
| [5] | Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver. |
| [6] | NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-94; ARG-126; ILE-217; VAL-247 AND THR-626. |
| [7] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [8] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-671 (ISOFORM 2). Tissue: Testis and Uterus. |
| [9] | Mihalek R., Homanics G.E. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-542 (ISOFORM 1). Tissue: Brain. |
| [10] | "The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle." Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C. J. Biol. Chem. 278:41535-41540(2003) [PubMed: 12909626] [Abstract] Cited for: IDENTIFICATION IN THE ORC COMPLEX, MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX. |
| [11] | "ATP-dependent assembly of the human origin recognition complex." Siddiqui K., Stillman B. J. Biol. Chem. 282:32370-32383(2007) [PubMed: 17716973] [Abstract] Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX. |
| [12] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208 AND SER-516, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [13] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [14] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Web resources
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF135044 mRNA. Translation: AAD30282.1. AF125507 mRNA. Translation: AAD18057.1. AF093535 mRNA. Translation: AAD40220.1. AL080116 mRNA. Translation: CAB45715.1. AK222795 mRNA. Translation: BAD96515.1. AY623113 Genomic DNA. Translation: AAT38109.1. AL451126, AL133211 Genomic DNA. Translation: CAI10900.1. AL133211, AL451126 Genomic DNA. Translation: CAI21539.1. AL451126, AL133211 Genomic DNA. Translation: CAM13063.1. AL133211, AL451126 Genomic DNA. Translation: CAM28255.1. BC035494 mRNA. Translation: AAH35494.1. BC047689 mRNA. Translation: AAH47689.1. Different termination. U50950 mRNA. Translation: AAA96313.1. |
| IPI | IPI00294402. IPI00374747. |
| PIR | T12452. |
| RefSeq | NP_036513.2. NM_012381.3. NP_862820.1. NM_181837.2. |
| UniGene | Hs.410228. |
3D structure databases | |
| ProteinModelPortal | Q9UBD5. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9UBD5. 14 interactions. |
| MINT | MINT-1201384. |
| STRING | Q9UBD5. |
PTM databases | |
| PhosphoSite | Q9UBD5. |
Polymorphism databases | |
| DMDM | 8928268. |
Proteomic databases | |
| PRIDE | Q9UBD5. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000392844; ENSP00000376586; ENSG00000135336. |
| GeneID | 23595. |
| KEGG | hsa:23595. |
| UCSC | uc003pmg.1. human. uc003pmh.1. human. |
Organism-specific databases | |
| CTD | 23595. |
| GeneCards | GC06P088299. |
| H-InvDB | HIX0006056. |
| HGNC | HGNC:8489. ORC3. |
| MIM | 604972. gene. |
| neXtProt | NX_Q9UBD5. |
| PharmGKB | PA32810. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG07201. |
| GeneTree | ENSGT00390000011376. |
| HOVERGEN | HBG007599. |
| OMA | SPIIIHR. |
| OrthoDB | EOG4X97GR. |
Enzyme and pathway databases | |
| Reactome | REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. |
Gene expression databases | |
| ArrayExpress | Q9UBD5. |
| Bgee | Q9UBD5. |
| Genevestigator | Q9UBD5. |
| GermOnline | ENSG00000135336. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR020795. ORC3. [Graphical view] |
| KO | K02605. |
| PANTHER | PTHR12748. ORC3. 1 hit. |
| Pfam | PF07034. ORC3_N. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 46252. |
| SOURCE | Search... |
Entry information
| Entry name | ORC3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9UBD5 Secondary accession number(s): A2A2T5 Q9UNT6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with