Q9UAY4 (ASM3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative sphingomyelin phosphodiesterase asm-3
EC=3.1.4.12

Gene names

Name:	asm-3
ORF Names:	W03G1.7

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts sphingomyelin to ceramide By similarity. UniProtKB Q10916
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate. UniProtKB Q10916
Subcellular location	Secreted By similarity UniProtKB Q10916.
Miscellaneous	There are two types of sphingomyelinases: asm (acid), and nsm (neutral). Only acid sphingomyelinase has been found in worms. UniProtKB P17405 UniProtKB Q10916
Sequence similarities	Belongs to the acid sphingomyelinase family. UniProtKB P17405 Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB determination of adult lifespan Inferred from mutant phenotype. Source: WormBase sphingomyelin catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 589	572	Putative sphingomyelin phosphodiesterase asm-3		PRO_0000002327
Regions
	Domain	18 – 101	84	Saposin B-type
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	237	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	463	1	N-linked (GlcNAc...) Potential
	Disulfide bond	21 ↔ 97		By similarity
	Disulfide bond	24 ↔ 89		By similarity
	Disulfide bond	52 ↔ 63		By similarity UniProtKB P17405
	Disulfide bond	154 ↔ 159		By similarity UniProtKB P17405
	Disulfide bond	160 ↔ 188		By similarity UniProtKB P17405
	Disulfide bond	530 ↔ 535		By similarity UniProtKB P17405
	Disulfide bond	541 ↔ 553		By similarity UniProtKB P17405

Sequences

	Sequence		Length	Mass (Da)
	Q9UAY4 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 7B23383D13062880	FASTA	589	67,451
	10 20 30 40 50 60 MLLGLLVLSL AFQGTLAVTE CEECKSIVDL LQFEWGEKKT EECVMEIAVF ICETFHIEDN 70 80 90 100 110 120 DVCNFIISDF SDEFMYVIKQ ILVTPHQLCG LLMKNDCGDF VDPLATIWNM TIPGNQPPFV 130 140 150 160 170 180 PKQVVPPGNP TLRALHLTDL HVDMFYTVGL EADCGTPQCC RPQDMNVEIV ENGDVKQPAG 190 200 210 220 230 240 PWGSVGSCDT PYWLLTNMLQ NIASTAGKLD YIMVSGDLVS HTVWAYTPET HSFMVKNLSD 250 260 270 280 290 300 TIRSYFPKTP VYFAVGNHEG VPVDNIAPHF TPKKYHMDWL YKAMSNAWQG WIPADQEKSL 310 320 330 340 350 360 EYNGCYMKKI YDGLRMISLN NVYGDRINFW LYINQTDPDG TLQWLINQLQ DAENVGDKVH 370 380 390 400 410 420 IVAHIPGSDG EALEGYALNY YKIINRYANT VVGQFFGHTH SEKFYMMYAN PDDYKSTPTN 430 440 450 460 470 480 VVYSAPSVTP YSDYFPAYRI YTIDGVHKGS TYQVIDYEEW FFNLTSNNAN PTNVKWEVLY 490 500 510 520 530 540 QSANMEYGLK GQIPTEYNQM IERMKTDDSL FNKYYENHNR RSIYDGRAPC NDQQCRNGYL 550 560 570 580 CDARQFHQTQ QLCTDLEGGI QKPEPKKNKY SARFATSNER RRGKEECKI

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081096 Genomic DNA. Translation: CCD69084.1.
PIR	T34001.
RefSeq	NP_001040996.1. NM_001047531.1.
UniGene	Cel.20210.
3D structure databases
ProteinModelPortal	Q9UAY4.
SMR	Q9UAY4. Positions 19-98, 195-261.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UAY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W03G1.7a; W03G1.7a; W03G1.7.
GeneID	176879.
KEGG	cel:W03G1.7.
NMPDR	fig|6239.3.peg.12318.
UCSC	W03G1.7b. c. elegans.
Organism-specific databases
CTD	176879.
WormBase	W03G1.7a; CE31626; WBGene00000213; asm-3.
Phylogenomic databases
eggNOG	meNOG05776.
GeneTree	EMGT00050000009609.
HOGENOM	HBG383897.
InParanoid	Q9UAY4.
OMA	HIVAHIP.
PhylomeDB	Q9UAY4.
Gene expression databases
ArrayExpress	Q9UAY4.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR011001. Saposin-like. IPR008139. SaposinB. IPR011160. Sphingomy_PDE. [Graphical view]
Gene3D	G3DSA:1.10.225.10. Saposin_like. 1 hit.
KO	K12350.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000948. Sphingomy_PDE. 1 hit.
SMART	SM00741. SapB. 1 hit. [Graphical view]
SUPFAM	SSF47862. Saposin_like. 1 hit.
PROSITE	PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	894418.

Entry information

Entry name

ASM3_CAEEL

Accession

Primary (citable) accession number: Q9UAY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project