Reviewed, UniProtKB/Swiss-Prot Q9UAE6 (ENO1_TOXGO)

Names and origin

Protein names

Recommended name:
    Enolase 1
    EC=4.2.1.11
Alternative name(s):
    2-phosphoglycerate dehydratase 1
    2-phospho-D-glycerate hydro-lyase 1

Gene names

Name:

ENO1

Organism

Toxoplasma gondii

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expressed preferentially in the bradyzoite stage. Ref.1
Miscellaneous	While ENO1 and ENO2 display similar K(m) values, the pure tachyzoite-specific enzyme (ENO2) has a threefold specific activity at V(max) compared with that of the bradyzoite-specific enolase (ENO1).
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Enolase 1		PRO_0000134093
Regions
	Region	382 – 385	4	Substrate binding By similarity
Sites
	Active site	217	1	Proton donor By similarity
	Active site	355	1	Proton acceptor By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	303	1	Substrate By similarity
	Binding site	330	1	Substrate By similarity
	Binding site	406	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UAE6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 667B3558B5B3D768	FASTA	444	48,341
	10 20 30 40 50 60 MVVIKDIVAR EILDSRGNPT IEVDVSTEGG VFRAAVPSGA STGIYEALEL RDKDPKRYLG 70 80 90 100 110 120 KGVLNAVEIV RQEIKPALLG KDPCDQKGID MLMVEQLDGT KNEWGYSKSK LGANAILGVS 130 140 150 160 170 180 IACCRAGAAS KGLPLYKYIA TLAGKTIDKM VMPVPFFNVI NGGEHAGNGL ALQEFLIAPV 190 200 210 220 230 240 GAPNIREAIR YGSETYHHLK NVIKNKYGLD ATNVGDEGGF APNVATAEEA LNLLVEAIKA 250 260 270 280 290 300 AGYEGKIKIA FDAAASEFYK QDEKKYDLDY KCKTKNASKH LTGEKLKEVY EGWLKKYPII 310 320 330 340 350 360 SVEDPFDQDD FASFSAFTKD VGEKTQVIGD DILVTNILRI EKALKDKACN CLLLKVNQIG 370 380 390 400 410 420 SVTEAIEACL LAQKSGWGVQ VSHRSGETED SFIADLVVGL RCGQIKSGSP CRSERLCKYN 430 440 QLMRIEESLG ADCVYAGESF RHPK

References

[1]	"The protozoan parasite Toxoplasma gondii expresses two functional plant-like glycolytic enzymes. Implications for evolutionary origin of apicomplexans." Dzierszinski F., Popescu O., Toursel C., Slomianny C., Yahiaoui B., Tomavo S. J. Biol. Chem. 274:24888-24895(1999) [PubMed: 10455162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"Toxoplasma gondii enolases ENO1 and ENO2 loci." Kibe M., Tomavo S. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PLK.
[3]	"Differential expression of two plant-like enolases with distinct enzymatic and antigenic properties during stage conversion of the protozoan parasite Toxoplasma gondii." Dzierszinski F., Mortuaire M., Dendouga N., Popescu O., Tomavo S. J. Mol. Biol. 309:1017-1027(2001) [PubMed: 11399076] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF051910 mRNA. Translation: AAD51128.1. AY155668 Genomic DNA. Translation: AAP24058.1.
3D structure databases
HSSP	HSSP built from PDB template 1OEP based on UniProtKB Q9NDH8.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.11. 262552.
Family and domain databases
InterPro	IPR000941. Enolase. [Graphical view]
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
ProDom	PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENO1_TOXGO

Accession

Primary (citable) accession number: Q9UAE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)