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Q9U9Y8

- NLK_CAEEL

UniProt

Q9U9Y8 - NLK_CAEEL

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Protein

Serine/threonine kinase NLK

Gene
lit-1, nlk, W06F12.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a role in the Wnt signaling pathway controlling the asymmetry of cell divisions during embryogenesis. Operates in the AB and EMS cell lineages influencing cell specification. Required for body wall muscle development, endoderm development, pop-1 asymmetry and T-cell division asymmetry. Lit-1/wrm-1 complex regulates pop-1 localization and is required for pop-1/par-5 interaction.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei269 – 2691ATP By similarity
Active sitei366 – 3661Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2549ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB
  5. protein kinase activity Source: WormBase
  6. protein N-terminus binding Source: UniProtKB
  7. protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  1. anatomical structure morphogenesis Source: WormBase
  2. asymmetric cell division Source: WormBase
  3. asymmetric protein localization involved in cell fate determination Source: WormBase
  4. embryo development Source: WormBase
  5. endodermal cell fate specification Source: WormBase
  6. endoderm development Source: UniProtKB
  7. muscle cell fate specification Source: WormBase
  8. polarity specification of proximal/distal axis Source: WormBase
  9. protein phosphorylation Source: WormBase
  10. Wnt signaling pathway Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_184336. Ca2+ pathway.
SignaLinkiQ9U9Y8.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine kinase NLK (EC:2.7.11.24)
Alternative name(s):
Loss of intestine protein 1
Nemo-like kinase
Gene namesi
Name:lit-1
Synonyms:nlk
ORF Names:W06F12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiW06F12.1a; CE29476; WBGene00003048; lit-1.
W06F12.1b; CE29477; WBGene00003048; lit-1.
W06F12.1c; CE29478; WBGene00003048; lit-1.
W06F12.1d; CE37160; WBGene00003048; lit-1.
W06F12.1e; CE37161; WBGene00003048; lit-1.

Subcellular locationi

Cytoplasmcell cortex. Nucleus
Note: Located in the anterior cell cortex before and during asymmetric cell division. After division, located preferentially in the nucleus of the posterior daughter cell.2 Publications

GO - Cellular componenti

  1. cell cortex Source: WormBase
  2. cytoplasm Source: WormBase
  3. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi357 – 3571L → S in t1512; intestine cells absent but have an excess of pharyngeal cells, symmetrical localization of pop-1 and symmetrical T-cell division. 3 Publications
Mutagenesisi400 – 4001T → A: No detectable kinase activity. 1 Publication
Mutagenesisi402 – 4021E → K in t1534; reduced body-wall muscle. 1 Publication
Mutagenesisi541 – 5411C → Y in or131; loss of pop-1 asymmetry and protruding vulva phenotype and defects in gonadal migration and development. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 634634Serine/threonine kinase NLKPRO_0000372802Add
BLAST

Proteomic databases

PaxDbiQ9U9Y8.
PRIDEiQ9U9Y8.

Expressioni

Tissue specificityi

Expressed in larval and adult pharynx and seam and vulval cells.2 Publications

Interactioni

Subunit structurei

Interacts with wrm-1 (via N-terminus); activates lit-1 kinase activity and the lit-1/wrm-1 dimer phosphorylates pop-1 which promotes pop-1 interaction with par-5 and translocation of pop-1 from the nucleus to the cytoplasm. Interacts with pop-1 (phosphorylated on 'Ser-118' and 'Ser-127'); dependent on lit-1/wrm-1 complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CELE_R02D5.1Q216365EBI-318513,EBI-329142
CELE_ZK1055.7O764493EBI-318513,EBI-313622
spe-44Q181713EBI-318513,EBI-320157
wrm-1Q109538EBI-318513,EBI-2530558

Protein-protein interaction databases

BioGridi41974. 64 interactions.
DIPiDIP-25624N.
IntActiQ9U9Y8. 23 interactions.
MINTiMINT-1045592.
STRINGi6239.W06F12.1a.

Structurei

3D structure databases

ProteinModelPortaliQ9U9Y8.
SMRiQ9U9Y8. Positions 214-595.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini240 – 531292Protein kinaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi80 – 216137Gln-richAdd
BLAST
Compositional biasi102 – 18887His-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000110881.
InParanoidiQ9U9Y8.
KOiK04468.
OMAiQHRYLEE.
OrthoDBiEOG72RMZ3.
PhylomeDBiQ9U9Y8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform a1 Publication (identifier: Q9U9Y8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVSWGRGKDA YYLYISREQE EDDDDSLSFY SSQKDTEDEF CGCLFPDPEI    50
PGSSSSSGCS SSSTELYDLA AAHAALISRQ QQILSQAIPI IPEHQLAAVA 100
AHHQHHQQLH PSVQYQLVAA ATHHNHHQPQ AAQPHYSAVV PRSDVIQQPP 150
HFALHHHLQN LVQQQQQQQA HHHHQQLVGE MALVSHTHPA AVGSTTCYEK 200
NQQKQQQVQQ IPTQPQVAHV SSNAILAAAQ PFYPPPVQDS QPDRPIGYGA 250
FGVVWSVTDP RSGKRVALKK MPNVFQNLAS CKRVFREIKM LSSFRHDNVL 300
SLLDILQPAN PSFFQELYVL TELMQSDLHK IIVSPQALTP DHVKVFVYQI 350
LRGLKYLHTA NILHRDIKPG NLLVNSNCIL KICDFGLART WDQRDRLNMT 400
HEVVTQYYRA PELLMGARRY TGAVDIWSVG CIFAELLQRK ILFQAAGPIE 450
QLQMIIDLLG TPSQEAMKYA CEGAKNHVLR AGLRAPDTQR LYKIASPDDK 500
NHEAVDLLQK LLHFDPDKRI SVEEALQHRY LEEGRLRFHS CMCSCCYTKP 550
NMPSRLFAQD LDPRHESPFD PKWEKDMSRL SMFELREKMY QFVMDRPALY 600
GVALCINPQS AAYKNFASSS VAQASELPPS PQAW 634
Length:634
Mass (Da):71,777
Last modified:May 1, 2000 - v1
Checksum:iA9F2EA7E3E2CE7F5
GO
Isoform b1 Publication (identifier: Q9U9Y8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     173-178: HHQQLV → MRDMIY

Show »
Length:462
Mass (Da):52,603
Checksum:iD030A48CA8F2968C
GO
Isoform c1 Publication (identifier: Q9U9Y8-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-197: Missing.
     198-216: YEKNQQKQQQVQQIPTQPQ → MILIAIIESFIEYLRKIVW

Note: No experimental confirmation available.

Show »
Length:437
Mass (Da):49,951
Checksum:i21C9CF88553F20D3
GO
Isoform d1 Publication (identifier: Q9U9Y8-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
     34-51: KDTEDEFCGCLFPDPEIP → MGRNQEGQFNGAGNSESA

Note: No experimental confirmation available.

Show »
Length:601
Mass (Da):67,652
Checksum:i311EED6DED5AA170
GO
Isoform e1 Publication (identifier: Q9U9Y8-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:454
Mass (Da):51,607
Checksum:iD951AB8C0CAC9394
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 197197Missing in isoform c. 1 PublicationVSP_052845Add
BLAST
Alternative sequencei1 – 180180Missing in isoform e. 1 PublicationVSP_052846Add
BLAST
Alternative sequencei1 – 172172Missing in isoform b. 1 PublicationVSP_052847Add
BLAST
Alternative sequencei1 – 3333Missing in isoform d. 1 PublicationVSP_052848Add
BLAST
Alternative sequencei34 – 5118KDTED…DPEIP → MGRNQEGQFNGAGNSESA in isoform d. 1 PublicationVSP_052849Add
BLAST
Alternative sequencei173 – 1786HHQQLV → MRDMIY in isoform b. 1 PublicationVSP_052850
Alternative sequencei198 – 21619YEKNQ…PTQPQ → MILIAIIESFIEYLRKIVW in isoform c. 1 PublicationVSP_052851Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF143243 mRNA. Translation: AAD37360.1.
AF143244 mRNA. Translation: AAD37361.1.
AF145376 mRNA. Translation: AAD39815.1.
Z83244 Genomic DNA. Translation: CAB05827.2.
Z83244 Genomic DNA. Translation: CAB60300.2.
Z83244 Genomic DNA. Translation: CAD18878.1.
Z83244 Genomic DNA. Translation: CAH10803.1.
Z83244, Z92822 Genomic DNA. Translation: CAH10804.1.
PIRiT26240.
RefSeqiNP_001022805.1. NM_001027634.2. [Q9U9Y8-1]
NP_001022806.1. NM_001027635.2. [Q9U9Y8-2]
NP_001022807.1. NM_001027636.2. [Q9U9Y8-3]
NP_001022808.1. NM_001027637.3. [Q9U9Y8-4]
NP_001022809.1. NM_001027638.3. [Q9U9Y8-5]
UniGeneiCel.6733.

Genome annotation databases

EnsemblMetazoaiW06F12.1a; W06F12.1a; WBGene00003048. [Q9U9Y8-1]
GeneIDi176808.
KEGGicel:CELE_W06F12.1.
UCSCiW06F12.1d. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF143243 mRNA. Translation: AAD37360.1 .
AF143244 mRNA. Translation: AAD37361.1 .
AF145376 mRNA. Translation: AAD39815.1 .
Z83244 Genomic DNA. Translation: CAB05827.2 .
Z83244 Genomic DNA. Translation: CAB60300.2 .
Z83244 Genomic DNA. Translation: CAD18878.1 .
Z83244 Genomic DNA. Translation: CAH10803.1 .
Z83244 , Z92822 Genomic DNA. Translation: CAH10804.1 .
PIRi T26240.
RefSeqi NP_001022805.1. NM_001027634.2. [Q9U9Y8-1 ]
NP_001022806.1. NM_001027635.2. [Q9U9Y8-2 ]
NP_001022807.1. NM_001027636.2. [Q9U9Y8-3 ]
NP_001022808.1. NM_001027637.3. [Q9U9Y8-4 ]
NP_001022809.1. NM_001027638.3. [Q9U9Y8-5 ]
UniGenei Cel.6733.

3D structure databases

ProteinModelPortali Q9U9Y8.
SMRi Q9U9Y8. Positions 214-595.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41974. 64 interactions.
DIPi DIP-25624N.
IntActi Q9U9Y8. 23 interactions.
MINTi MINT-1045592.
STRINGi 6239.W06F12.1a.

Proteomic databases

PaxDbi Q9U9Y8.
PRIDEi Q9U9Y8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai W06F12.1a ; W06F12.1a ; WBGene00003048 . [Q9U9Y8-1 ]
GeneIDi 176808.
KEGGi cel:CELE_W06F12.1.
UCSCi W06F12.1d. c. elegans.

Organism-specific databases

CTDi 176808.
WormBasei W06F12.1a ; CE29476 ; WBGene00003048 ; lit-1.
W06F12.1b ; CE29477 ; WBGene00003048 ; lit-1.
W06F12.1c ; CE29478 ; WBGene00003048 ; lit-1.
W06F12.1d ; CE37160 ; WBGene00003048 ; lit-1.
W06F12.1e ; CE37161 ; WBGene00003048 ; lit-1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000110881.
InParanoidi Q9U9Y8.
KOi K04468.
OMAi QHRYLEE.
OrthoDBi EOG72RMZ3.
PhylomeDBi Q9U9Y8.

Enzyme and pathway databases

Reactomei REACT_184336. Ca2+ pathway.
SignaLinki Q9U9Y8.

Miscellaneous databases

NextBioi 894088.
PROi Q9U9Y8.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans."
    Rocheleau C.E., Yasuda J., Shin T.H., Lin R., Sawa H., Okano H., Priess J.R., Davis R.J., Mello C.C.
    Cell 97:717-726(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND E), FUNCTION, INTERACTION WITH WRM-1, MUTAGENESIS OF LEU-357.
  2. "MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans."
    Meneghini M.D., Ishitani T., Carter J.C., Hisamoto N., Ninomiya-Tsuji J., Thorpe C.J., Hamill D.R., Matsumoto K., Bowerman B.
    Nature 399:793-797(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, MUTAGENESIS OF CYS-541.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  4. "Binary specification of the embryonic lineage in Caenorhabditis elegans."
    Kaletta T., Schnabel H., Schnabel R.
    Nature 390:294-298(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LEU-357 AND GLU-402.
  5. "MOM-4, a MAP kinase kinase kinase-related protein, activates WRM-1/LIT-1 kinase to transduce anterior/posterior polarity signals in C. elegans."
    Shin T.H., Yasuda J., Rocheleau C.E., Lin R., Soto M., Bei Y., Davis R.J., Mello C.C.
    Mol. Cell 4:275-280(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-400.
  6. "Left-right asymmetry in C. elegans intestine organogenesis involves a LIN-12/Notch signaling pathway."
    Hermann G.J., Leung B., Priess J.R.
    Development 127:3429-3440(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Phosphorylation by the beta-catenin/MAPK complex promotes 14-3-3-mediated nuclear export of TCF/POP-1 in signal-responsive cells in C. elegans."
    Lo M.-C., Gay F., Odom R., Shi Y., Lin R.
    Cell 117:95-106(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH POP-1.
  8. "Identification of C. elegans DAF-12-binding sites, response elements, and target genes."
    Shostak Y., Van Gilst M.R., Antebi A., Yamamoto K.R.
    Genes Dev. 18:2529-2544(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, MUTAGENESIS OF CYS-541.
  9. "Asymmetric cortical and nuclear localizations of WRM-1/beta-catenin during asymmetric cell division in C. elegans."
    Takeshita H., Sawa H.
    Genes Dev. 19:1743-1748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNLK_CAEEL
AccessioniPrimary (citable) accession number: Q9U9Y8
Secondary accession number(s): O62395
, Q69YW9, Q8WQB7, Q9U343, Q9UA07, Q9Y198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

Worms lacking nlk exhibit defects in body wall muscle, endoderm development and pop-1 asymmetry.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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