Reviewed, UniProtKB/Swiss-Prot Q9U9K0 (ADD1_CAEEL)

Names and origin

Protein names

Recommended name:
    Adducin-related protein 1

Gene names

Name:	add-1
ORF Names:	F39C12.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the aldolase class II family. Adducin subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Ligand	Actin-binding
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform b (identifier: Q9U9K0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform a (identifier: Q9U9K0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     665-679: CDHPSPMSISTEYPE → YPDILRPIYRQETYV
     680-732: Missing.

Isoform c (identifier: Q9U9K0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     666-695: Missing.

Isoform d (identifier: Q9U9K0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     645-694: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Adducin-related protein 1		PRO_0000218540
Natural variations
	Alternative sequence	645 – 694	50	Missing in isoform d.		VSP_000193
	Alternative sequence	665 – 679	15	CDHPS…TEYPE → YPDILRPIYRQETYV in isoform a.		VSP_000194
	Alternative sequence	666 – 695	30	Missing in isoform c.		VSP_000195
	Alternative sequence	680 – 732	53	Missing in isoform a.		VSP_000196
Experimental info
	Sequence conflict	377	1	P → S in AAD49856. Ref.1
	Sequence conflict	377	1	P → S in AAD49857. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified August 2, 2002. Version 2. Checksum: CBEA2AAD053C6322	FASTA	732	82,170
	10 20 30 40 50 60 MIGRKEKERE RPYYRDPDDP EYIKDLQRPA VIKEDLSEME RRKRVQQILE SKSFCHELEE 70 80 90 100 110 120 VIRQECDTAR TDPDHLQVLQ KLSDLTVPQG NMSFGNLHTY GGNTIAIADL RGNEKYSKAE 130 140 150 160 170 180 RIQRNKLACL FRLADLFQWS QGIHNEISYR TNDEDNTFLM NPFGLLYHEI TAATIVKIDE 190 200 210 220 230 240 NGKILDCGTL KAGVNQPAFL LHSAIYKAHP MVRCILHMHT AIVAAVASMK CGLLPLCKEA 250 260 270 280 290 300 MVLGPVGYHD YQDIGDDDIQ FDEIIANLGD KNVLFLRNQG FLVVGDTIEH ATFLANNTVI 310 320 330 340 350 360 ACETQVRAAR AGLDNLIIPE EKAIQRAFRN SRNTNSLKRN GTVDWRVGEL EWESWMRVLD 370 380 390 400 410 420 HANFQTGHVY RQPQLRPKSA MSTSMVNNND VAVPPTTSAY GQIDETNLES VSAHRLALLR 430 440 450 460 470 480 KEQERVRWMN SPNAYQKVEF LEYGADNPKK ITKWVHDVNV PSASGTPVKI SSVHQFSPAS 490 500 510 520 530 540 SNPKEFKEKQ KAIKENNRLG TLSAGPQSQI LDSVTYEDIA LLIKPDNDGT VGQSSTADRA 550 560 570 580 590 600 ILIGTASKGI IDRQFQHHAQ VYHQIYAPNP FSVETDADIK KYVDMVKAKN SQSAPVSARS 610 620 630 640 650 660 GYSQYDEVEA DTVSLMQGVR EHKLSQAALS ASDDGLNAGI SPNNVRTSEE SVNTSYMSQS 670 680 690 700 710 720 VVFDCDHPSP MSISTEYPEK VKMTRFSSTQ GTSEGNTTSR SCTTASEEEK PTKDEKKKKK 730 KGFLSFMRKK DK
	Isoform a. Checksum: 692841752A61D8AD	FASTA	679	76,373
	10 20 30 40 50 60 MIGRKEKERE RPYYRDPDDP EYIKDLQRPA VIKEDLSEME RRKRVQQILE SKSFCHELEE 70 80 90 100 110 120 VIRQECDTAR TDPDHLQVLQ KLSDLTVPQG NMSFGNLHTY GGNTIAIADL RGNEKYSKAE 130 140 150 160 170 180 RIQRNKLACL FRLADLFQWS QGIHNEISYR TNDEDNTFLM NPFGLLYHEI TAATIVKIDE 190 200 210 220 230 240 NGKILDCGTL KAGVNQPAFL LHSAIYKAHP MVRCILHMHT AIVAAVASMK CGLLPLCKEA 250 260 270 280 290 300 MVLGPVGYHD YQDIGDDDIQ FDEIIANLGD KNVLFLRNQG FLVVGDTIEH ATFLANNTVI 310 320 330 340 350 360 ACETQVRAAR AGLDNLIIPE EKAIQRAFRN SRNTNSLKRN GTVDWRVGEL EWESWMRVLD 370 380 390 400 410 420 HANFQTGHVY RQPQLRPKSA MSTSMVNNND VAVPPTTSAY GQIDETNLES VSAHRLALLR 430 440 450 460 470 480 KEQERVRWMN SPNAYQKVEF LEYGADNPKK ITKWVHDVNV PSASGTPVKI SSVHQFSPAS 490 500 510 520 530 540 SNPKEFKEKQ KAIKENNRLG TLSAGPQSQI LDSVTYEDIA LLIKPDNDGT VGQSSTADRA 550 560 570 580 590 600 ILIGTASKGI IDRQFQHHAQ VYHQIYAPNP FSVETDADIK KYVDMVKAKN SQSAPVSARS 610 620 630 640 650 660 GYSQYDEVEA DTVSLMQGVR EHKLSQAALS ASDDGLNAGI SPNNVRTSEE SVNTSYMSQS 670 VVFDYPDILR PIYRQETYV
	Isoform c. Checksum: 200652C0AE243303	FASTA	702	78,872
	10 20 30 40 50 60 MIGRKEKERE RPYYRDPDDP EYIKDLQRPA VIKEDLSEME RRKRVQQILE SKSFCHELEE 70 80 90 100 110 120 VIRQECDTAR TDPDHLQVLQ KLSDLTVPQG NMSFGNLHTY GGNTIAIADL RGNEKYSKAE 130 140 150 160 170 180 RIQRNKLACL FRLADLFQWS QGIHNEISYR TNDEDNTFLM NPFGLLYHEI TAATIVKIDE 190 200 210 220 230 240 NGKILDCGTL KAGVNQPAFL LHSAIYKAHP MVRCILHMHT AIVAAVASMK CGLLPLCKEA 250 260 270 280 290 300 MVLGPVGYHD YQDIGDDDIQ FDEIIANLGD KNVLFLRNQG FLVVGDTIEH ATFLANNTVI 310 320 330 340 350 360 ACETQVRAAR AGLDNLIIPE EKAIQRAFRN SRNTNSLKRN GTVDWRVGEL EWESWMRVLD 370 380 390 400 410 420 HANFQTGHVY RQPQLRPKSA MSTSMVNNND VAVPPTTSAY GQIDETNLES VSAHRLALLR 430 440 450 460 470 480 KEQERVRWMN SPNAYQKVEF LEYGADNPKK ITKWVHDVNV PSASGTPVKI SSVHQFSPAS 490 500 510 520 530 540 SNPKEFKEKQ KAIKENNRLG TLSAGPQSQI LDSVTYEDIA LLIKPDNDGT VGQSSTADRA 550 560 570 580 590 600 ILIGTASKGI IDRQFQHHAQ VYHQIYAPNP FSVETDADIK KYVDMVKAKN SQSAPVSARS 610 620 630 640 650 660 GYSQYDEVEA DTVSLMQGVR EHKLSQAALS ASDDGLNAGI SPNNVRTSEE SVNTSYMSQS 670 680 690 700 VVFDCNTTSR SCTTASEEEK PTKDEKKKKK KGFLSFMRKK DK
	Isoform d. Checksum: 1FA125B56ED80D33	FASTA	682	76,578
	10 20 30 40 50 60 MIGRKEKERE RPYYRDPDDP EYIKDLQRPA VIKEDLSEME RRKRVQQILE SKSFCHELEE 70 80 90 100 110 120 VIRQECDTAR TDPDHLQVLQ KLSDLTVPQG NMSFGNLHTY GGNTIAIADL RGNEKYSKAE 130 140 150 160 170 180 RIQRNKLACL FRLADLFQWS QGIHNEISYR TNDEDNTFLM NPFGLLYHEI TAATIVKIDE 190 200 210 220 230 240 NGKILDCGTL KAGVNQPAFL LHSAIYKAHP MVRCILHMHT AIVAAVASMK CGLLPLCKEA 250 260 270 280 290 300 MVLGPVGYHD YQDIGDDDIQ FDEIIANLGD KNVLFLRNQG FLVVGDTIEH ATFLANNTVI 310 320 330 340 350 360 ACETQVRAAR AGLDNLIIPE EKAIQRAFRN SRNTNSLKRN GTVDWRVGEL EWESWMRVLD 370 380 390 400 410 420 HANFQTGHVY RQPQLRPKSA MSTSMVNNND VAVPPTTSAY GQIDETNLES VSAHRLALLR 430 440 450 460 470 480 KEQERVRWMN SPNAYQKVEF LEYGADNPKK ITKWVHDVNV PSASGTPVKI SSVHQFSPAS 490 500 510 520 530 540 SNPKEFKEKQ KAIKENNRLG TLSAGPQSQI LDSVTYEDIA LLIKPDNDGT VGQSSTADRA 550 560 570 580 590 600 ILIGTASKGI IDRQFQHHAQ VYHQIYAPNP FSVETDADIK KYVDMVKAKN SQSAPVSARS 610 620 630 640 650 660 GYSQYDEVEA DTVSLMQGVR EHKLSQAALS ASDDGLNAGI SPNNGNTTSR SCTTASEEEK 670 680 PTKDEKKKKK KGFLSFMRKK DK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional analysis of the spectrin based membrane skeleton in Caenorhabditis elegans." Moorthy S., Bennett V. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF166167 mRNA. Translation: AAD49856.1. AF166168 mRNA. Translation: AAD49857.1. AF039043 Genomic DNA. Translation: AAK70638.1. AF039043 Genomic DNA. Translation: AAK70639.1. AF039043 Genomic DNA. Translation: AAL32219.1. AF039043 Genomic DNA. Translation: AAM54181.1.
RefSeq	NP_001024639.1. NP_001024640.1. NP_001024641.1. NP_001024642.1.
UniGene	Cel.7980
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9U9K0. 7 interactions.
STRING	Q9U9K0.
Proteomic databases
PRIDE	Q9U9K0.
Genome annotation databases
Ensembl	F39C12.2b; F39C12.2b; F39C12.2; Caenorhabditis elegans. [Genome view]
GeneID	180760.
KEGG	cel:F39C12.2.
UCSC	F39C12.2b. c. elegans.
Organism-specific databases
CTD	180760.
WormBase	WBGene00000072. add-1.
WormPep	F39C12.2a. CE28308. [WorfDB] F39C12.2b. CE28309. [WorfDB] F39C12.2c. CE29794. [WorfDB] F39C12.2d. CE30979. [WorfDB]
Phylogenomic databases
HOGENOM	HBG388064.
InParanoid	Q9U9K0.
OMA	TTSAYGQ.
Gene expression databases
ArrayExpress	Q9U9K0.
Family and domain databases
InterPro	IPR001303. Aldolase_II/adducin_N. [Graphical view]
Gene3D	G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit.
Pfam	PF00596. Aldolase_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	910812.

Entry information

Entry name

ADD1_CAEEL

Accession

Primary (citable) accession number: Q9U9K0
Secondary accession number(s): O44581, Q95X64, Q9U9J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 2, 2002
Last modified:	December 15, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project