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Protein

Neuropathy target esterase sws

Gene

sws

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain.3 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei985NucleophilePROSITE-ProRule annotation1
Active sitei1105Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 301cNMP 1Add BLAST128
Nucleotide bindingi482 – 609cNMP 2Add BLAST128
Nucleotide bindingi598 – 727cNMP 3Add BLAST130

GO - Molecular functioni

  • lysophospholipase activity Source: UniProtKB
  • protein kinase A catalytic subunit binding Source: FlyBase

GO - Biological processi

  • developmental process Source: GO_Central
  • ensheathment of neurons Source: FlyBase
  • glial cell apoptotic process Source: UniProtKB
  • membrane lipid metabolic process Source: FlyBase
  • membrane organization Source: FlyBase
  • negative regulation of cAMP-dependent protein kinase activity Source: FlyBase
  • neuron apoptotic process Source: FlyBase
  • phosphatidylcholine metabolic process Source: FlyBase
  • phospholipid metabolic process Source: FlyBase
  • photoreceptor cell maintenance Source: FlyBase
  • protein localization to membrane Source: FlyBase
  • sensory perception of smell Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Neurogenesis

Enzyme and pathway databases

ReactomeiR-DME-6814848. Glycerophospholipid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropathy target esterase sws
Alternative name(s):
Swiss cheese (EC:3.1.1.5)
Short name:
DSWS
Gene namesi
Name:sws
ORF Names:CG2212
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0003656. sws.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34LumenalSequence analysisAdd BLAST34
Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
Topological domaini56 – 1425CytoplasmicSequence analysisAdd BLAST1370

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Progressive degeneration of the adult nervous system, associated with apoptotic cell death, glial hyperwrapping, and neuronal apoptosis. Also, vacuolization in the neuropil.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133R → A: Has no effect on nervous system function but reduces binding to Pka-C3. 1 Publication1
Mutagenesisi648G → R in allele sws-5; age-dependent neurodegeneration. 1 Publication1
Mutagenesisi956G → D in allele sws-4; age-dependent neurodegeneration. 1 Publication1
Mutagenesisi985S → D: Loss of enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001725271 – 1425Neuropathy target esterase swsAdd BLAST1425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei444Phosphoserine1 Publication1
Modified residuei453Phosphoserine1 Publication1
Modified residuei1160Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9U969.
PRIDEiQ9U969.

PTM databases

iPTMnetiQ9U969.

Expressioni

Tissue specificityi

Isoform A and isoform B are expressed in the entire brain cortex; cortical cell bodies of adult brain. Sws and Pka-C3 are colocalized in all neurons.3 Publications

Developmental stagei

Isoform A is expressed in all developmental stages with highest levels in young embryos and adults. Isoform B is detected only in adult head.1 Publication

Gene expression databases

BgeeiFBgn0003656.
ExpressionAtlasiQ9U969. baseline.
GenevisibleiQ9U969. DM.

Interactioni

Subunit structurei

Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.1 Publication

GO - Molecular functioni

  • protein kinase A catalytic subunit binding Source: FlyBase

Protein-protein interaction databases

BioGridi58188. 1 interactor.
IntActiQ9U969. 2 interactors.
STRINGi7227.FBpp0071077.

Structurei

3D structure databases

ProteinModelPortaliQ9U969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini952 – 1118PNPLAPROSITE-ProRule annotationAdd BLAST167

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi956 – 961GXGXXGPROSITE-ProRule annotation6
Motifi983 – 987GXSXGPROSITE-ProRule annotation5
Motifi1105 – 1107DGA/GPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 3 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
GeneTreeiENSGT00390000002533.
InParanoidiQ9U969.
KOiK14676.
OrthoDBiEOG091G00XM.
PhylomeDBiQ9U969.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 3 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 3 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 3 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9U969-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVLEMLRAS ASGSYNTIFS DAWCQYVSKQ ITATVYMYFA LVMMSLLFIA
60 70 80 90 100
WFLYFKRMAR LRLRDEIARS ISTVTNSSGD MRGLRFRKRD KMLFYGRRML
110 120 130 140 150
RKMKNVSGQM YSSGKGYKRR AVMRFARRIL QLRRDNMPLE MRTVEPPAEY
160 170 180 190 200
LEETIEGSDR VPPDALYMLQ SIRIFGHFEK PVFLRLCKHT QLLELMAGDY
210 220 230 240 250
LFKITDPDDS VYIVQSGMIN VYISNADGST LSLKTVRKGE SVTSLLSFID
260 270 280 290 300
VLSGNPSYYK TVTAKAIEKS VVIRLPMQAF EEVFQDNPDV MIRVIQVIMI
310 320 330 340 350
RLQRVLFTAL RNYLGLNAEL VQNHMRYKSV STMSGPINSQ TSQSSRQAPN
360 370 380 390 400
GPPMVISQMN LMQSAVSGTG SSGVSVTVTR PPSSPSRHSR EEHTLSDPNP
410 420 430 440 450
NPDGSFHGTT NLFTEVHGDA PNADLFHQQQ QQHSVGNLST RRSSITLMAP
460 470 480 490 500
DGSHSCLQTP GVTTSIDMRL VQSSAVDSLR KELGLSEEDS HIIEPFVELR
510 520 530 540 550
ELEPNVTLIT EGNADDVCVW FVMTGTLAVY QSNQDATRAK QDKSDMLIHF
560 570 580 590 600
VHPGEIVGGL AMLTGEASAY TIRSRSITRI AFIRRAAIYQ IMRQRPRIVL
610 620 630 640 650
DLGNGVVRRL SPLVRQCDYA LDWIFLESGR AVYRQDESSD STYIVLSGRM
660 670 680 690 700
RSVITHPGGK KEIVGEYGKG DLVGIVEMIT ETSRTTTVMA VRDSELAKLP
710 720 730 740 750
EGLFNAIKLR YPIVVTKLIS FLSHRFLGSM QTRSGSGAPG APVEANPVTH
760 770 780 790 800
KYSTVALVPI TDEVPMTPFT YELYHSLCAI GPVLRLTSDV VRKQLGSNIF
810 820 830 840 850
EAANEYRLTS WLAQQEDRNI ITLYQCDSSL SAWTQRCMRQ ADVILIVGLG
860 870 880 890 900
DRSHLVGKFE REIDRLAMRT QKELVLLYPE ASNAKPANTL SWLNARPWVT
910 920 930 940 950
KHHHVLCVKR IFTRKSQYRI NDLYSRVLLS EPNMHSDFSR LARWLTGNSI
960 970 980 990 1000
GLVLGGGGAR GAAHIGMLKA IQEAGIPVDM VGGVSIGALM GALWCSERNI
1010 1020 1030 1040 1050
TTVTQKAREW SKKMTKWFLQ LLDLTYPITS MFSGREFNKT IHDTFGDVSI
1060 1070 1080 1090 1100
EDLWIPYFTL TTDITASCHR IHTNGSLWRY VRSSMSLSGY MPPLCDPKDG
1110 1120 1130 1140 1150
HLLLDGGYVN NLPADVMHNL GAAHIIAIDV GSQDDTDLTN YGDDLSGWWL
1160 1170 1180 1190 1200
LYKKWNPFTS PVKVPDLPDI QSRLAYVSCV RQLEEVKNSD YCEYIRPPID
1210 1220 1230 1240 1250
KYKTLAFGSF DEIRDVGYVF GKNYFESMAK AGRLGRFNQW FNKEPPKRVN
1260 1270 1280 1290 1300
HASLNEYTFI DLAQIVCRLP ETYAVNTAEL FSEDEDCDGY ISEPTTLNTD
1310 1320 1330 1340 1350
RRRIQVSRAG NSLSFSETEM DSDVELDLKL ERKTDKSTQS SPPSNSRSDM
1360 1370 1380 1390 1400
RGKEEARHMS NWHWGVKHKD ETGSGATEAT KTQTGQEQEL QQEQQDQGAT
1410 1420
AEQLVDKDKE ENKENRSSPN NETKN
Length:1,425
Mass (Da):160,573
Last modified:March 25, 2003 - v2
Checksum:iA7FEE533B1C2B628
GO
Isoform B (identifier: Q9U969-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-1084: Missing.
     1087-1098: LSGYMPPLCDPK → IAGVFPPFCDYR
     1107-1112: GYVNNL → CYTNNV

Show »
Length:341
Mass (Da):38,784
Checksum:i27425E1327272860
GO
Isoform C (identifier: Q9U969-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1087-1098: LSGYMPPLCDPK → IAGVFPPFCDYR
     1107-1112: GYVNNL → CYTNNV

Note: No experimental confirmation available.
Show »
Length:1,425
Mass (Da):160,671
Checksum:i98FBA2A5E1A368D6
GO

Sequence cautioni

The sequence AAL28979 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18I → T in CAB51772 (PubMed:9295388).Curated1
Sequence conflicti314L → F in ABV82201 (Ref. 4) Curated1
Sequence conflicti452G → P in CAB51772 (PubMed:9295388).Curated1
Sequence conflicti785R → H in CAB51772 (PubMed:9295388).Curated1
Sequence conflicti1076S → H in ABV82201 (Ref. 4) Curated1
Sequence conflicti1081 – 1083VRS → CRA in ABV82201 (Ref. 4) Curated3
Sequence conflicti1377T → N in CAB51772 (PubMed:9295388).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383981 – 1084Missing in isoform B. CuratedAdd BLAST1084
Alternative sequenceiVSP_0383991087 – 1098LSGYM…LCDPK → IAGVFPPFCDYR in isoform B and isoform C. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_0384001107 – 1112GYVNNL → CYTNNV in isoform B and isoform C. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97187 mRNA. Translation: CAB51772.1.
AE014298 Genomic DNA. Translation: AAF46305.3.
AE014298 Genomic DNA. Translation: AAN09223.1.
BT030819 mRNA. Translation: ABV82201.1.
AY061431 mRNA. Translation: AAL28979.1. Different initiation.
RefSeqiNP_511075.3. NM_078520.3. [Q9U969-1]
NP_727225.1. NM_167141.3. [Q9U969-2]
UniGeneiDm.3604.

Genome annotation databases

EnsemblMetazoaiFBtr0071125; FBpp0071077; FBgn0003656. [Q9U969-1]
GeneIDi31716.
KEGGidme:Dmel_CG2212.
UCSCiCG2212-RB. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97187 mRNA. Translation: CAB51772.1.
AE014298 Genomic DNA. Translation: AAF46305.3.
AE014298 Genomic DNA. Translation: AAN09223.1.
BT030819 mRNA. Translation: ABV82201.1.
AY061431 mRNA. Translation: AAL28979.1. Different initiation.
RefSeqiNP_511075.3. NM_078520.3. [Q9U969-1]
NP_727225.1. NM_167141.3. [Q9U969-2]
UniGeneiDm.3604.

3D structure databases

ProteinModelPortaliQ9U969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58188. 1 interactor.
IntActiQ9U969. 2 interactors.
STRINGi7227.FBpp0071077.

PTM databases

iPTMnetiQ9U969.

Proteomic databases

PaxDbiQ9U969.
PRIDEiQ9U969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0071125; FBpp0071077; FBgn0003656. [Q9U969-1]
GeneIDi31716.
KEGGidme:Dmel_CG2212.
UCSCiCG2212-RB. d. melanogaster.

Organism-specific databases

CTDi31716.
FlyBaseiFBgn0003656. sws.

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
GeneTreeiENSGT00390000002533.
InParanoidiQ9U969.
KOiK14676.
OrthoDBiEOG091G00XM.
PhylomeDBiQ9U969.

Enzyme and pathway databases

ReactomeiR-DME-6814848. Glycerophospholipid catabolism.

Miscellaneous databases

GenomeRNAii31716.
PROiQ9U969.

Gene expression databases

BgeeiFBgn0003656.
ExpressionAtlasiQ9U969. baseline.
GenevisibleiQ9U969. DM.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 3 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 3 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 3 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSWS_DROME
AccessioniPrimary (citable) accession number: Q9U969
Secondary accession number(s): A8E6M7
, Q8IRN7, Q95RE9, Q9W3M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 25, 2003
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.