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Q9U943

- APLP_LOCMI

UniProt

Q9U943 - APLP_LOCMI

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Protein
Apolipophorins
Gene
N/A
Organism
Locusta migratoria (Migratory locust)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Constitutes the major component of lipophorin, which mediates transport for various types of lipids in hemolymph. Acts by forming lipoprotein particles that bind lipoproteins and lipids. May be required for morphogens wingless (wg) and hedgehog (hh) function, possibly by acting as vehicles for the movement of wg and hh By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei720 – 7212Cleavage; by furin

GO - Molecular functioni

  1. lipid binding Source: UniProtKB-KW
  2. lipid transporter activity Source: InterPro

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport, Wnt signaling pathway

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipophorins
Cleaved into the following 2 chains:
Alternative name(s):
Apolipophorin II
apoLp-2
Alternative name(s):
Apolipophorin I
apoLp-1
OrganismiLocusta migratoria (Migratory locust)
Taxonomic identifieri7004 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaOrthopteraCaeliferaAcridomorphaAcridoideaAcrididaeOedipodinaeLocusta

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi717 – 7171R → D: Abolishes cleavage by furin. 1 Publication
Mutagenesisi717 – 7171R → Q: Partially affects cleavage by furin. 1 Publication
Mutagenesisi719 – 7191K → A: Does not affect cleavage by furin. 1 Publication
Mutagenesisi720 – 7201R → Q: Abolishes cleavage by furin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 Publication
Add
BLAST
Chaini22 – 720699Apolipophorin-2
PRO_0000041528Add
BLAST
Chaini721 – 33802660Apolipophorin-1
PRO_0000041529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi132 – 1321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi649 – 6491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi969 – 9691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2174 – 21741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2851 – 28511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi3177 – 31771N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Cleaved into 2 chains by furin protease. However, prevention of cleavage does not impair its function.
N-glycosylated By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Expressioni

Tissue specificityi

Present in brain, hemolymph, fat body and eyes.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 646607Vitellogenin
Add
BLAST
Domaini2816 – 3012197VWFD
Add
BLAST

Sequence similaritiesi

Contains 1 VWFD domain.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.25.10.20. 1 hit.
2.20.50.20. 2 hits.
2.20.80.10. 1 hit.
2.30.230.10. 1 hit.
InterProiIPR015819. Lipid_transp_b-sht_shell.
IPR001747. Lipid_transpt_N.
IPR009454. Lipid_transpt_open_b-sht.
IPR015816. Vitellinogen_b-sht_N.
IPR015255. Vitellinogen_open_b-sht.
IPR015817. Vitellinogen_open_b-sht_sub1.
IPR015818. Vitellinogen_open_b-sht_sub2.
IPR011030. Vitellinogen_superhlx.
IPR001846. VWF_type-D.
[Graphical view]
PfamiPF06448. DUF1081. 1 hit.
PF09172. DUF1943. 1 hit.
PF01347. Vitellogenin_N. 1 hit.
PF00094. VWD. 1 hit.
[Graphical view]
SMARTiSM00638. LPD_N. 1 hit.
SM00216. VWD. 1 hit.
[Graphical view]
SUPFAMiSSF48431. SSF48431. 1 hit.
SSF56968. SSF56968. 3 hits.
PROSITEiPS51211. VITELLOGENIN. 1 hit.
PS51233. VWFD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9U943-1 [UniParc]FASTAAdd to Basket

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MGTPPHIWFL LILAISSGGL SAAVGGCNHQ CTPQSSVFQY QKGQTYTYSF     50
EGTTLTSLPG TQGEPVRLKL KATADLSVAD DCNKVLRLRG VTVSGPDSKN 100
YANLKDLEAH PVLANFKGSS INKQLCSEDG DNQSSLNIKR AILSLLQTPN 150
TKSSTASEVD VFGICPTNVR HSQRGDVTVI SKTRNLNRYA SRENLIQETL 200
STRFTGQSDL HATPFLDADL HVEQQIKGGL IVSATSRESY LFRPFSNQGN 250
GAKTIVETKL TLTSQNAQPA PPLASFTVPK SIVFEAPHAL ASVPGGSSAI 300
TAALHAAESS TKDGVTVDAA EKFRTLVSVL RQSSTTDILK VYNDVKAGAG 350
FSNKHSARNL LLDALFRTST GDAVEVIARL LKTKEITANH WYLSLAFIQH 400
ASLKSVVSIS SLLDQKNLPT EAFLGIGSFI GRYCREHNCE NVAEFDEVLN 450
KFSKHLSGST TSKAGENRAI AALKALGNIR HLNNALGEKV KQLALDKSLP 500
PRVRVAALEV IQSDPCRKNI KQAALQILRD QVEDSELRIK AYLAVVECPC 550
DNVVKTISNL LENEPIIQVG SFVVSHLKNL QASTDPSKAE AKEKLGQLKP 600
KKIFSSDIRK YSQNYELSYA IDAINAGASV ESNVIFSQSS YLPRSVSLNL 650
TADVFGHSYN VFEIAARTEN LDHIIESFLG PKGYIETEDD DKFVDEVEEK 700
TKSLYNRITE RFEKTFRQKR SVSKDAVDNI RQQAYKSLLP SQRDRSLDVD 750
LSLKTFGSEL AWFNYDGKHE QKSSERVVDE IFDAIDEGLK KSKKFNYDFE 800
PHFTFLDSEL SYPTNLGFPL KLAIDGSIAA RLKLNGEVDV RSILRQPENA 850
AFRLEFVPSA AVELTGKLLV DAYVVEGGLK LDYNVHSSTG INVAVHNLND 900
LGIDIKVGLP VKKQDIIDVK TDVLTTVKER GHPETSTPLH FNLKGNDYKQ 950
YRGCFDQLSP VSGLTFCGNV SVPWVSPTQA AAFYPLNGPS HLSVSIEADD 1000
VSEYHFRAEI KKDESAFKSA AVLFDTPGSS ADRKVLLLVE KKEKPHQGIT 1050
AHLKSGWKEI VAEGLLIDDN NEKSVSAKLV IESDEYSIKG GVKISGNPSR 1100
QVYKPILEYK APAKDSGAKV KKSHKTSEGI TVDGAVVVER TSDKGKYTFQ 1150
DLSLKTPKGT FVINGQLDIV PRNYAFDLKL SVDKNELLLN GHLNYAEPKS 1200
IDVALEVTSP QFPDYGSGFQ LINKRGDDYS DTKIILACGR DLKSDGSRLI 1250
LEHFIKGKYE TPDTFNLETK GEVLGTGHKI FGKFDIDSKP KHLEYDLKLG 1300
FDENEVTSDL VAKRDIKSPD DYELKFSAKI LDNSIRIESS REVKPKDDSA 1350
FLNTLVVLSG KKYEFQVDVK LAAEDEYHTS LKAESNLKIE GKTSVRLITD 1400
FTTDAQTVNG HVKVSNEGED FFELIYKLNR GSGNPSGNAK LFVKNYLDGA 1450
ATFKYNNGVG SGTLQIDVLK LHRKIKATGD LTLSGSQRSA AIDLYWDADR 1500
DQSKQLLFKT ENDVKEKSID SKNTLKILDK LTTLNFKGSL SGAIDDGEVE 1550
GQGELILPVG TYLGVKFGRA LHLTQADTKV GLHLQAEGRE SASSTQPVWK 1600
SDFLLESALT RDSFVGEAKL LFETKGKDDL KLFLSGKSLP QGEKKLISGQ 1650
FSGQGSLIGG RTSVIKLNSE IDETFIAYNL NSECNQGYRA NIVGKINRGY 1700
SPVAVKQIEN TLELLLPFDK LKQLKHTIIG TFSSQPESTP EFTVSNVIIW 1750
NNENTLKLTG EAAGDEKEGR TKWDLILPKE EPRTLETTWS NAGDNKKAGS 1800
LSFKWGGNKE AKVSTDIEFT SDNQPQILHL KATSPTEKFG IFDLALSLKK 1850
NADPADKIDF ELTVTADQKK TDVKGSLGLA PGVPIIDVVA VQPSGTSKVF 1900
VDFLRKSDSE LHGAIELQWV AFGGGHLTAN GDIKLDIDDF YLKLDVDSPK 1950
FNFNKWHLEA GQRAAKGSKR IVFTAKSAEK VLFSGSTNFH SKAENNKISY 2000
SGNGQVRIGD KAHAFNFRSS RQNLIQDANK EIGVEYNLDF KIAGHGSLHN 2050
ILKVTNKELH ALGKQCSEGK PNCAVVEIKS KVSAADAKET THDLVFLVDL 2100
KSVGVDTGVA FTAETVRRGF WLIDEQASLT LSHNGETTYK YKGYLKESGS 2150
GFTLTLPSRV IAAEVKLSSD VKPNHSKQQI SASVWLDKTR LPNSFSSVSI 2200
LLEEIEDKNT DKYVSQLRFT HPNLEKDLTV KGYVQIGLEN KLFDSNLEID 2250
IFKQKNQKIS ISSTVVEQKQ NDVVKYLSTL DVKSKGHELD VTGRGEATVK 2300
PSLIALQSVL KYKKDKRIKE FKNFQFEVST EKLLVHVKVP NHHLLHIDAN 2350
TKINDKHASG DASVHIIGLP TSVIHIEGEN KGFPVVKGTI SSEGTPNKLE 2400
LIADLSDGLL VEADFISESG KKELFYTFLS GKKDSRKPEF RWSVENIQSA 2450
LEPHKNDIQE VLNKLKEISD EAGNEITKES SRLADSLKAG LPNFRRFVNT 2500
YETQLKALKE EIANDKVLKE ISENWKEVIG DAAEVVSTLV NGILVTIDAL 2550
LKTLNELAES VLDALKKSLP ALKDSYKQAV DAIVGIAKSL TQSLVNILSS 2600
AAEILKKHEA DIKEYLSVLA DLANDVGKFV TKITGVIYEG VVEFSKPIKE 2650
KLDGLKFGVA IEFGKVVEQL QNLIVPQELL AFAQEIVSEL KETTLTPEIQ 2700
DLLQAIEKYL EKVSKKKDAD VEKELKLIFE KAIDAVESVI NFVVSEITGG 2750
DHTKDLYDIN IPTVLPSFIQ LPRVFSVRFS PLIYLVSNGV PCLSDLLASY 2800
RPSLRFDNII PPYDATAILL NSHHFFTFDR RHLTFKGICS YILAQDVQDG 2850
NFTIIANIEG GSLKSIIVSD QATTFELASD KSLLVNGRPT EYPADEGEFH 2900
AWREYNRVGI QTKAGVKVTC ETSIELCTFE INGFYFGKTR GLLGTINNEP 2950
WDDFTKPDGQ VASKANEFGN AWKVDAQCAN VDGVDHHEHS IKVEECEEVF 3000
SKASLLSPCS LFLDPAPYLE ACSHIAHEAT TKEEKQLAAC RTAAAYVQAC 3050
SVENVFVSVP PHCVHCSVNG DAAIDIGQSF SVKVPQKSAD ILIVLEQVTG 3100
NAETVKDFVS PIVSQLTQEL SSRGISDVWI SLLGYGAPGQ EYPHLYTSSG 3150
GKLSYDGKQK NIQFGERKVL GPFPFDNFTE SIDWLDEFTD QAFHLITTAD 3200
TILDYPFRPG AAKSIIYVLD TSCETTLFLK HLPVKALKLK DAIGSPGIVL 3250
HLVTNVDSVQ SKNIVGFDTN HAYYNQEGKK RVVSEVTGNE KAALKISETA 3300
CGQIALATSG TVFNKNNLKQ TKKFVAQHIA DSLTNVELTQ DCKCLPVEGI 3350
HTRAVCAVTG AREKEHLSVK GVKGTKGVKG 3380
Length:3,380
Mass (Da):371,809
Last modified:July 10, 2007 - v2
Checksum:i62526A263A041111
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ130944 mRNA. Translation: CAB51918.2.

Cross-referencesi

Web resourcesi

Protein Spotlight

Lipid freight - Issue 59 of June 2005

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ130944 mRNA. Translation: CAB51918.2 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.25.10.20. 1 hit.
2.20.50.20. 2 hits.
2.20.80.10. 1 hit.
2.30.230.10. 1 hit.
InterProi IPR015819. Lipid_transp_b-sht_shell.
IPR001747. Lipid_transpt_N.
IPR009454. Lipid_transpt_open_b-sht.
IPR015816. Vitellinogen_b-sht_N.
IPR015255. Vitellinogen_open_b-sht.
IPR015817. Vitellinogen_open_b-sht_sub1.
IPR015818. Vitellinogen_open_b-sht_sub2.
IPR011030. Vitellinogen_superhlx.
IPR001846. VWF_type-D.
[Graphical view ]
Pfami PF06448. DUF1081. 1 hit.
PF09172. DUF1943. 1 hit.
PF01347. Vitellogenin_N. 1 hit.
PF00094. VWD. 1 hit.
[Graphical view ]
SMARTi SM00638. LPD_N. 1 hit.
SM00216. VWD. 1 hit.
[Graphical view ]
SUPFAMi SSF48431. SSF48431. 1 hit.
SSF56968. SSF56968. 3 hits.
PROSITEi PS51211. VITELLOGENIN. 1 hit.
PS51233. VWFD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and gene expression in the eye of the apolipophorin II/I precursor from Locusta migratoria."
    Bogerd J., Babin P.J., Kooiman F.P., Andre M., Ballagny C., van Marrewijk W.J., van der Horst D.J.
    J. Comp. Neurol. 427:546-558(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-33; 100-117; 204-228; 637-645; 668-682; 721-737; 1299-1308; 1489-1498 AND 2232-2242, TISSUE SPECIFICITY.
    Tissue: Fat body.
  2. Bogerd J.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 131; 140; 156; 189; 412-413; 494; 1087; 1103; 1559; 1646-1650; 2614; 2736; 2764; 2888; 3263 AND 3302.
  3. "Biosynthesis and secretion of insect lipoprotein: involvement of furin in cleavage of the apoB homolog, apolipophorin-II/I."
    Smolenaars M.M.W., Kasperaitis M.A.M., Richardson P.E., Rodenburg K.W., Van der Horst D.J.
    J. Lipid Res. 46:412-421(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY FURIN, MUTAGENESIS OF ARG-717; LYS-719 AND ARG-720.

Entry informationi

Entry nameiAPLP_LOCMI
AccessioniPrimary (citable) accession number: Q9U943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3