Reviewed, UniProtKB/Swiss-Prot Q9U8W8 (TL5A_TACTR)

Names and origin

Protein names	Recommended name:     Techylectin-5A
Organism	Tachypleus tridentatus (Japanese horseshoe crab)
Taxonomic identifier	6853 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Merostomata › Xiphosura › Limulidae › Tachypleus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lectin involved in innate immunity. Agglutinates all types of human erythrocytes, Gram-positive and Gram-negative bacteria. Has a stronger agglutinating activity towards Gram-negative bacteria than towards Gram-positive bacteria. Specifically recognizes acetyl group-containing substances on agglutinated cells. The hemagglutinating activity was inhibited by EDTA, acetyl group-containing mono- and disaccharides, N-acetyl derivatives of amino acids, other acetyl group-containing substances, propionamide and benzamide. Enhances the antimicrobial activity of big defensin against Gram-positive bacteria but not against Gram-negative bacteria. Ref.1
Subunit structure	Multimeric. Ref.1 and Ref.2 are in disagreement about the nature of the multimer, Ref.1 finds hexamers and octamers, the results in Ref.2 suggest tetramers. Ref.1 Ref.2
Subcellular location	Secreted.
Tissue specificity	Strongly expressed in heart and intestine, weakly expressed in hepatopancreas. Not found in hemocytes, stomach, nervous tissue or skeletal muscle. Ref.1
Sequence similarities	Contains 1 fibrinogen C-terminal domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Lectin Metal-binding
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell adhesion Ref.1 Inferred from direct assay. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor binding Inferred from electronic annotation. Source: InterPro sugar binding Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.1

Chain

24 – 292

269

Techylectin-5A Ref.1

PRO_0000260045

Regions

Domain

63 – 286

224

Fibrinogen C-terminal

Sites

Metal binding

221

1

Calcium Ref.2

Metal binding

225

1

Calcium Ref.2

Metal binding

227

1

Calcium Ref.2

Site

209

1

Implicated in ligand binding Ref.2

Site

233

1

Implicated in ligand binding Ref.2

Site

243

1

Implicated in ligand binding Ref.2

Site

259

1

Implicated in ligand binding Ref.2

Site

260

1

Implicated in ligand binding Ref.2

Site

271

1

Implicated in ligand binding Ref.2

Amino acid modifications

Modified residue

24

1

Pyrrolidone carboxylic acid Ref.1

Glycosylation

173

1

N-linked (GlcNAc...) Potential

Glycosylation

198

1

N-linked (GlcNAc...) Potential

Glycosylation

214

1

N-linked (GlcNAc...) Potential

Disulfide bond

72 ↔ 103

Ref.2

Disulfide bond

229 ↔ 242

Ref.2

Secondary structure

1

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292

Helix Strand Turn

Details...

Helix

72 – 77

6

Beta strand

84 – 88

5

Helix

93 – 96

4

Beta strand

99 – 104

6

Helix

107 – 109

3

Beta strand

112 – 118

7

Turn

125 – 128

4

Helix

132 – 137

6

Beta strand

144 – 147

4

Helix

150 – 159

10

Beta strand

162 – 169

8

Beta strand

175 – 185

11

Helix

188 – 190

3

Beta strand

199 – 203

5

Helix

208 – 210

3

Beta strand

223 – 227

5

Helix

229 – 233

5

Beta strand

240 – 242

3

Beta strand

257 – 260

4

Helix

266 – 269

4

Beta strand

272 – 274

3

Beta strand

276 – 284

9

Sequences

	Sequence		Length	Mass (Da)
	Q9U8W8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D028DE1E2716F71A	FASTA	292	33,787
	10 20 30 40 50 60 MHNLRNILFV ITLIGQKYGL TSSQNKELCD VTSSTGLLDS IKVMASHVKE QLKDKGTSEV 70 80 90 100 110 120 AQPIVSPDPT DCADILLNGY RSSGGYRIWP KSWMTVGTLN VYCDMETDGG GWTVIQRRGN 130 140 150 160 170 180 YGNPSDYFYK PWKNYKLGFG NIEKDFWLGN DRIFALTNQR NYMIRFDLKD KENDTRYAIY 190 200 210 220 230 240 QDFWIENEDY LYCLHIGNYS GDAGNSFGRH NGHNFSTIDK DHDTHETHCA QTYKGGWWYD 250 260 270 280 290 RCHESNLNGL YLNGEHNSYA DGIEWRAWKG YHYSLPQVEM KIRPVEFNII GN

References

[1]

"Horseshoe crab acetyl group-recognizing lectins involved in innate immunity are structurally related to fibrinogen." Gokudan S., Muta T., Tsuda R., Koori K., Kawahara T., Seki N., Mizunoe Y., Wai S.N., Iwanaga S., Kawabata S. Proc. Natl. Acad. Sci. U.S.A. 96:10086-10091(1999) [PubMed: 10468566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-31; 104-109 AND 275-280, FUNCTION, SUBUNIT, TISSUE SPECIFICITY, PYROGLUTAMATE FORMATION AT GLN-24. Tissue: Heart.

[2]

"The 2.0-A crystal structure of tachylectin 5A provides evidence for the common origin of the innate immunity and the blood coagulation systems." Kairies N., Beisel H.-G., Fuentes-Prior P., Tsuda R., Muta T., Iwanaga S., Bode W., Huber R., Kawabata S. Proc. Natl. Acad. Sci. U.S.A. 98:13519-13524(2001) [PubMed: 11707569] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 24-292, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB024737 mRNA. Translation: BAA84188.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JC9	X-ray	2.01	A	24-292	[»]

SMR

Q9U8W8. Positions 3-292.

ModBase

Search...

Family and domain databases

InterPro

IPR002181. Fibrinogen_a/b/g_C.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR020837. Fibrinogen_CS.
[Graphical view]

Gene3D

G3DSA:3.90.215.10. Fibrinogen_a/b/g_C_1. 1 hit.

Pfam

PF00147. Fibrinogen_C. 1 hit.
[Graphical view]

SMART

SM00186. FBG. 1 hit.
[Graphical view]

PROSITE

PS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TL5A_TACTR

Accession

Primary (citable) accession number: Q9U8W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)