Reviewed, UniProtKB/Swiss-Prot Q9U793 (PSA2_TRYBB)

Names and origin

Protein names	Recommended name:     Proteasome subunit alpha type-2     EC=3.4.25.1 Alternative name(s):     20S proteasome subunit alpha-2
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Proteasome subunit alpha type-2		PRO_0000124084

Sequences

	Sequence		Length	Mass (Da)
	Q9U793-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 80026D6FC40F8429	FASTA	231	25,355
	10 20 30 40 50 60 MSESSYGLTT FSPSGRLVQI EYATTAASKG TTALGVKATD GVVIAAEKKT TSPLADSLTL 70 80 90 100 110 120 HKVFALDDHV GCTYSGIGPD CRVLVDAARR ACQRYRLTYH EPMPISQLVR QISFLFQEFT 130 140 150 160 170 180 QSGGVRPFGC SLLVAGADSR GNHLYQLDPS GTFWTWKATS IGKGSPDART FLEKRYTNEM 190 200 210 220 230 EIEDAVHTAL LTLKEGFDGR MTAENTQVGR VVEGRFELLT VEQLKDYLDQ I

References

[1]

"Functional assignment of the 20 S proteasome from Trypanosoma brucei using mass spectrometry and new bioinformatics approaches." Huang L., Jacob R.J., Pegg S.C.H., Baldwin M.A., Wang C.C., Burlingame A.L., Babbitt P.C. J. Biol. Chem. 276:28327-28339(2001) [PubMed: 11309374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 427.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF148125 mRNA. Translation: AAF05906.1.
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P23639.
ModBase	Search...
Protein family/group databases
MEROPS	T01.972.
Phylogenomic databases
OMA	HIALLTL.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA2_TRYBB

Accession

Primary (citable) accession number: Q9U793

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)