Reviewed, UniProtKB/Swiss-Prot Q9U720 (DCSA_DICDI)

Names and origin

Protein names

Recommended name:
    Cellulose synthase catalytic subunit A [UDP-forming]
    EC=2.4.1.12

Gene names

Name:	dcsA
ORF Names:	DDB_G0269124

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1059 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component for mechanical and chemical protection at the onset of the stalk formation, when the cells exhibit multicellular behavior during culmination. Ref.1
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Pathway	Glycan metabolism; amoeba cellulose biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Developmental stage	Progressively accumulates during culmination, especially at the onset of the stalk formation. Present in fruiting body (at protein level). Ref.1
Domain	There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Miscellaneous	Amoebae missing dcsA exhibit snowmen-shaped fruiting bodies.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Developmental protein Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1059	1059	Cellulose synthase catalytic subunit A [UDP-forming]		PRO_0000327825
Regions
	Transmembrane	246 – 266	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	306 – 323	18	Potential
	Transmembrane	790 – 810	21	Potential
	Transmembrane	813 – 833	21	Potential
	Transmembrane	963 – 983	21	Potential
	Transmembrane	993 – 1013	21	Potential
	Transmembrane	1035 – 1055	21	Potential
	Region	328 – 628	301	Catalytic subdomain A By similarity
	Region	701 – 761	61	Catalytic subdomain B By similarity
Sites
	Active site	370	1	Potential
	Active site	717	1	Potential
	Binding site	624	1	Substrate Potential
	Binding site	626	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9U720-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2CF8A701D7D99B81	FASTA	1,059	121,412
	10 20 30 40 50 60 MDRNEGGDFP INTPNNINSS GGSYNNSMNN SSNNIGRDIG NNQSSRNLKP KPSQSNLKWI 70 80 90 100 110 120 ARDLKKKSVR KDSERKLKSS GVLKKKNTVM DFGEDDGGSG DDGNITEGLP ISEGMDDLPS 130 140 150 160 170 180 SSNSRGGSGN DEQKKQFPKE MNSPSSEYGT TSGGQRFDTL VDPDISLAEM EEKMRQHKVY 190 200 210 220 230 240 QEQQQQQQQQ QQQQKQKDKE LSSQKKKPSS MQLSKKKHVA KEDSETLETI IGEEKKEVVF 250 260 270 280 290 300 EVKPYFSHAI LQATMAVFLI WNIFYFAYRA GWTMNRTDYI TFSYSILFII VEFISFLGSA 310 320 330 340 350 360 LHLNNFTNPC TFVLVVTLEQ ILAKRRKKHP TVMMYVCTYK EPPSIVSRTF RTAISMDYPS 370 380 390 400 410 420 ENLWIGLLDD SVNYRESRGW AHLQSVEKNF LYVLLQKAVY SVHNIRPPVT SQHEDPHGIL 430 440 450 460 470 480 NETSSKIESS TKEVIEAEVQ WFIEYFLLNS WFGVGQEIPR DADDAERALI AKLRDDNFSP 490 500 510 520 530 540 YRTFTKSESE KISNFTIDSL QSLWHGSAFF RPLIRSILLK KDYVRNFVSE LNNQHRLRFL 550 560 570 580 590 600 NTEALAMAQY QVLMMGRQEL PWDEISSGNV RIDFDTCDGP IVSPKCTYLR RRKPPIPHNK 610 620 630 640 650 660 AGNINNALFN ESTKADYEFL GLLDADQQPH PDFLKRVLPY FYSDEGQDLA FVQTPQFFSN 670 680 690 700 710 720 IYPVDDPLGH RNMEFYGPVM EGRSANNACP FVGTNAIFRR QPLYDIGGIM YNSVTEDMYT 730 740 750 760 770 780 GMKLQVSGYK SWYHNEVLVV GTAPVDLKET LEQRKRWAQG AVEIFSLTPW GYIRGKLGWR 790 800 810 820 830 840 KMLYNLDSCI YPFLSPTAFF YGASPLIMSI WTVPIVVKDP IIFILVGMIP VMVLPRVIQY 850 860 870 880 890 900 MILRAKRPYE AGKSGPSLWV EATDLWRAEQ TFFGFAGTYI SSWREGSASI VKLLKARKIS 910 920 930 940 950 960 RHKLAMWNWK RDFVKKPVVC EVFRQTKLVN ENDNAQESSG KHKAEQSFRT SNKESDTIKN 970 980 990 1000 1010 1020 SRLFLPNIIL FVVNILAMMS AVLRFNCFQN DMWLLVVVAG FSFSTLWHLW SFIPMALRQS 1030 1040 1050 EKQWPYASSY HAHNIVLFLV LGFLVLLFVD VKVCIPRVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cellulose synthase gene of Dictyostelium." Blanton R.L., Fuller D., Iranfar N., Grimson M.J., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 97:2391-2396(2000) [PubMed: 10681463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF163835 mRNA. Translation: AAF00200.1. AAFI02000005 Genomic DNA. Translation: EAL71912.1.
RefSeq	XP_646256.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	3397696.
KEGG	ddi:DDB_0191159.
Organism-specific databases
dictyBase	DDB_G0269124. dcsA.
Enzyme and pathway databases
BRENDA	2.4.1.12. 424.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCSA_DICDI

Accession

Primary (citable) accession number: Q9U720
Secondary accession number(s): Q55D75

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)