Q9U6Y8 (RFP_DISSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Red fluorescent protein drFP583 Short name=DsRed
Organism	Discosoma sp. (Sea anemone)
Taxonomic identifier	86600 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Corallimorpharia › Discosomatidae › Discosoma

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. Ref.1
Subunit structure	Homotetramer. Ref.3
Post-translational modification	Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. Ref.3
Biotechnological use	Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Sequence similarities	Belongs to the GFP family.
Biophysicochemical properties	Absorption: Abs(max)=558 nm Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm.

Ontologies

Keywords
Biological process	Luminescence
Ligand	Chromophore
Molecular function	Photoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 225

225

Red fluorescent protein drFP583

PRO_0000192577

Amino acid modifications

Modified residue

67

1

(Z)-2,3-didehydrotyrosine Ref.3

Cross-link

66 ↔ 68

2-iminomethyl-5-imidazolinone (Gln-Gly) Ref.3

Secondary structure

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225

Helix Strand Turn

Details...

Beta strand

10 – 22

13

Beta strand

25 – 36

12

Turn

37 – 40

4

Beta strand

41 – 51

11

Helix

58 – 60

3

Helix

62 – 64

3

Helix

70 – 72

3

Helix

82 – 85

4

Turn

86 – 89

4

Beta strand

91 – 99

9

Beta strand

104 – 114

11

Beta strand

117 – 127

11

Turn

134 – 138

5

Beta strand

140 – 143

4

Beta strand

146 – 153

8

Beta strand

156 – 167

12

Beta strand

170 – 185

16

Beta strand

192 – 204

13

Beta strand

208 – 220

13

Sequences

	Sequence		Length	Mass (Da)
	Q9U6Y8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FBF9A5369778F689	FASTA	225	25,931
	10 20 30 40 50 60 MRSSKNVIKE FMRFKVRMEG TVNGHEFEIE GEGEGRPYEG HNTVKLKVTK GGPLPFAWDI 70 80 90 100 110 120 LSPQFQYGSK VYVKHPADIP DYKKLSFPEG FKWERVMNFE DGGVVTVTQD SSLQDGCFIY 130 140 150 160 170 180 KVKFIGVNFP SDGPVMQKKT MGWEASTERL YPRDGVLKGE IHKALKLKDG GHYLVEFKSI 190 200 210 220 YMAKKPVQLP GYYYVDSKLD ITSHNEDYTI VEQYERTEGR HHLFL

References

[1]	"Fluorescent proteins from nonbioluminescent Anthozoa species." Matz M.V., Fradkov A.F., Labas Y.A., Savitsky A.P., Zaraisky A.G., Markelov M.L., Lukyanov S.A. Nat. Biotechnol. 17:969-973(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The structural basis for red fluorescence in the tetrameric GFP homolog DsRed." Wall M.A., Socolich M., Ranganathan R. Nat. Struct. Biol. 7:1133-1138(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[3]	"Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution." Yarbrough D., Wachter R.M., Kallio K., Matz M.V., Remington S.J. Proc. Natl. Acad. Sci. U.S.A. 98:462-467(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF168419 mRNA. Translation: AAF03369.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G7K	X-ray	2.00	A/B/C/D	2-225	[»]
1GGX	X-ray	1.90	A/B/C/D	1-225	[»]
1ZGO	X-ray	1.40	A/B/C/D	1-225	[»]
1ZGP	X-ray	1.90	A/B/C/D	1-225	[»]
1ZGQ	X-ray	1.90	A/B/C/D/E/F/G/H	1-225	[»]
2H5O	X-ray	1.08	A/B	8-221	[»]
2H5R	X-ray	1.60	A	8-221	[»]
2H8Q	X-ray	2.00	A/B/C/D	7-225	[»]
2V4E	X-ray	2.40	A/B/C/D/E/F/G/H	6-225	[»]
2VAD	X-ray	1.59	A	7-215	[»]
2VAE	X-ray	1.64	A/B/C/D/E/F/G/H	7-225	[»]

ProteinModelPortal

Q9U6Y8.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.40.155.10. 1 hit.

InterPro

IPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
IPR000786. Green_fluorescent_prot.
[Graphical view]

Pfam

PF01353. GFP. 1 hit.
[Graphical view]

PRINTS

PR01229. GFLUORESCENT.

SUPFAM

SSF54511. GFP_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9U6Y8.

Entry information

Entry name

RFP_DISSP

Accession

Primary (citable) accession number: Q9U6Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)