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Q9U6Y8 (RFP_DISSP) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Red fluorescent protein drFP583

Short name=DsRed
OrganismDiscosoma sp. (Sea anemone)
Taxonomic identifier86600 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaAnthozoaHexacoralliaCorallimorphariaDiscosomatidaeDiscosoma

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. Ref.1

Subunit structure

Homotetramer. Ref.3

Post-translational modification

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. Ref.3

Biotechnological use

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Sequence similarities

Belongs to the GFP family.

Biophysicochemical properties

Absorption:

Abs(max)=558 nm

Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm.

Ontologies

Keywords
   Biological processLuminescence
   LigandChromophore
   Molecular functionPhotoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

generation of precursor metabolites and energy

Inferred from electronic annotation. Source: InterPro

protein-chromophore linkage

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Red fluorescent protein drFP583
PRO_0000192577

Amino acid modifications

Modified residue671(Z)-2,3-didehydrotyrosine Ref.3
Cross-link66 ↔ 682-iminomethyl-5-imidazolinone (Gln-Gly) Ref.3

Secondary structure

.................................... 225
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9U6Y8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: FBF9A5369778F689

FASTA22525,931
        10         20         30         40         50         60 
MRSSKNVIKE FMRFKVRMEG TVNGHEFEIE GEGEGRPYEG HNTVKLKVTK GGPLPFAWDI 

        70         80         90        100        110        120 
LSPQFQYGSK VYVKHPADIP DYKKLSFPEG FKWERVMNFE DGGVVTVTQD SSLQDGCFIY 

       130        140        150        160        170        180 
KVKFIGVNFP SDGPVMQKKT MGWEASTERL YPRDGVLKGE IHKALKLKDG GHYLVEFKSI 

       190        200        210        220 
YMAKKPVQLP GYYYVDSKLD ITSHNEDYTI VEQYERTEGR HHLFL 

« Hide

References

[1]"Fluorescent proteins from nonbioluminescent Anthozoa species."
Matz M.V., Fradkov A.F., Labas Y.A., Savitsky A.P., Zaraisky A.G., Markelov M.L., Lukyanov S.A.
Nat. Biotechnol. 17:969-973(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The structural basis for red fluorescence in the tetrameric GFP homolog DsRed."
Wall M.A., Socolich M., Ranganathan R.
Nat. Struct. Biol. 7:1133-1138(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[3]"Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution."
Yarbrough D., Wachter R.M., Kallio K., Matz M.V., Remington S.J.
Proc. Natl. Acad. Sci. U.S.A. 98:462-467(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF168419 mRNA. Translation: AAF03369.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G7KX-ray2.00A/B/C/D2-225[»]
1GGXX-ray1.90A/B/C/D1-225[»]
1ZGOX-ray1.40A/B/C/D1-225[»]
1ZGPX-ray1.90A/B/C/D1-225[»]
1ZGQX-ray1.90A/B/C/D/E/F/G/H1-225[»]
2H5OX-ray1.08A/B8-221[»]
2H5RX-ray1.60A8-221[»]
2H8QX-ray2.00A/B/C/D7-225[»]
2V4EX-ray2.40A/B/C/D/E/F/G/H6-225[»]
2VADX-ray1.59A7-215[»]
2VAEX-ray1.64A/B/C/D/E/F/G/H7-225[»]
4I2YX-ray2.20A/B148-221[»]
4KF4X-ray1.99A/B/C/D/E/F/G/H8-221[»]
4KF5X-ray2.60C/D8-221[»]
ProteinModelPortalQ9U6Y8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.155.10. 1 hit.
InterProIPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
IPR000786. Green_fluorescent_prot.
[Graphical view]
PfamPF01353. GFP. 1 hit.
[Graphical view]
PRINTSPR01229. GFLUORESCENT.
SUPFAMSSF54511. SSF54511. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9U6Y8.

Entry information

Entry nameRFP_DISSP
AccessionPrimary (citable) accession number: Q9U6Y8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 2000
Last modified: March 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references