Q9U6D2 (JNK1_ANCCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stress-activated protein kinase JNK-1
EC=2.7.11.24

Gene names

Name:

JNK-1

Organism

Ancylostoma caninum (Dog hookworm)

Taxonomic identifier

29170 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Strongylida › Ancylostomatoidea › Ancylostomatidae › Ancylostomatinae › Ancylostoma

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity By similarity. UniProtKB P45983
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB P45983
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity. UniProtKB P45983
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAF00539.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	JNK cascade Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Stress-activated protein kinase JNK-1		PRO_0000186269
Regions
	Domain	26 – 321	296	Protein kinase UniProtKB P53779
	Nucleotide binding	33 – 38	6	ATP By similarity UniProtKB P53779
	Motif	183 – 185	3	TXY
Sites
	Active site	151	1	Proton acceptor By similarity UniProtKB P53779
	Binding site	55	1	ATP By similarity UniProtKB P53779
Amino acid modifications
	Modified residue	183	1	Phosphothreonine By similarity UniProtKB P53779
	Modified residue	185	1	Phosphotyrosine By similarity UniProtKB P53779

Sequences

	Sequence		Length	Mass (Da)
	Q9U6D2 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 2EDFA15CC28AD3A4	FASTA	376	42,770
	10 20 30 40 50 60 MASTSVPENY YVVPIGETQM VILKRYENLR LIGSGAQGIV CAATDMVTNK PVAIKKLSRP 70 80 90 100 110 120 FQNVTHAKRA YREFILMNLV NHKNIIGLLN AFSPQREVEE FNDLYIVMEL MDANLCQVIQ 130 140 150 160 170 180 MDLDHERLSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAVEAF 190 200 210 220 230 240 MMTPYVVTRY YRAPEVILGM GYGENVDVWS VGCIFGELIR GRVLFPGADH IDQWTRIIEL 250 260 270 280 290 300 LGTPEPQFLA RLQTTVRNYV ENRQKYQPVP FETLFADNMF PPGADNARLT AAKARDLLSR 310 320 330 340 350 360 MLVIDPEKRI SVDDALAHEY VNVWYDASRG SCSSAGPSTI LLVDGEHTVE EWRAMLFAEL 370 QNYYRTHDVY GVGQRR

References

[1]	"JNK-1 kinase of the hookworm nematode, Ancylostoma caninum." Arasu P. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF187690 mRNA. Translation: AAF00539.1. Different initiation.
3D structure databases
ProteinModelPortal	Q9U6D2.
SMR	Q9U6D2. Positions 10-362.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008351. JNK_MAPK. IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JNK1_ANCCA

Accession

Primary (citable) accession number: Q9U6D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2002
Last modified:	July 27, 2011
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)