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Protein

Calcium release-activated calcium channel protein 1

Gene

olf186-F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores. Regulates transcription factor NFAT nuclear import.2 Publications

Miscellaneous

In Greek mythology, the 'Orai' are the keepers of the gates of heaven: Eunomia (order or harmony), Dike (justice) and Eirene (peace).

GO - Molecular functioni

  • store-operated calcium channel activity Source: UniProtKB

GO - Biological processi

  • nervous system development Source: FlyBase
  • positive regulation of calcium ion transport Source: UniProtKB
  • positive regulation of NFAT protein import into nucleus Source: UniProtKB
  • regulation of cardiac conduction Source: Reactome
  • store-operated calcium entry Source: FlyBase

Keywordsi

Molecular functionCalcium channel, Ion channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Enzyme and pathway databases

ReactomeiR-DME-139853. Elevation of cytosolic Ca2+ levels.
R-DME-5578775. Ion homeostasis.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Protein family/group databases

TCDBi1.A.52.1.5. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai
dOrai
Gene namesi
Name:olf186-F
Synonyms:CRACM1, olf186
ORF Names:CG11430
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0041585. olf186-F.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 163CytoplasmicSequence analysisAdd BLAST163
Transmembranei164 – 181HelicalSequence analysisAdd BLAST18
Topological domaini182 – 191ExtracellularSequence analysis10
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 248CytoplasmicSequence analysisAdd BLAST36
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 277ExtracellularSequence analysis8
Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
Topological domaini299 – 351CytoplasmicSequence analysisAdd BLAST53

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002343881 – 351Calcium release-activated calcium channel protein 1Add BLAST351

Proteomic databases

PaxDbiQ9U6B8.
PRIDEiQ9U6B8.

Expressioni

Gene expression databases

BgeeiFBgn0041585.
ExpressionAtlasiQ9U6B8. differential.
GenevisibleiQ9U6B8. DM.

Interactioni

Protein-protein interaction databases

BioGridi62725. 3 interactors.
DIPiDIP-59770N.
IntActiQ9U6B8. 1 interactor.
MINTiMINT-1600663.
STRINGi7227.FBpp0113106.

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi145 – 179Combined sources35
Helixi192 – 215Combined sources24
Turni216 – 218Combined sources3
Helixi237 – 271Combined sources35
Turni272 – 274Combined sources3
Helixi276 – 285Combined sources10
Helixi287 – 306Combined sources20
Helixi308 – 331Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HKRX-ray3.35A/B133-341[»]
4HKSX-ray3.35A/B133-341[»]
ProteinModelPortaliQ9U6B8.
SMRiQ9U6B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi109 – 114Poly-Pro6

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
GeneTreeiENSGT00390000015354.
InParanoidiQ9U6B8.
KOiK16056.
OMAiFSKPRRI.
OrthoDBiEOG091G0E4P.

Family and domain databases

InterProiView protein in InterPro
IPR012446. CRAC_channel.
PANTHERiPTHR31501. PTHR31501. 1 hit.
PfamiView protein in Pfam
PF07856. Orai-1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AImported (identifier: Q9U6B8-1) [UniParc]FASTAAdd to basket
Also known as: BImported, FImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVWTTANNS GLETPTKSPI TSSVPRAARS SAVITTGNHQ QHHFQHVVAA
60 70 80 90 100
AVAAATSVAT GHQFQQQFPL HAHPHPQHHS NSPTGSGSNS NNSAGFQRTS
110 120 130 140 150
ISNSLLQFPP PPPPSSQNQA KPRGHHRTAS SSMSQSGEDL HSPTYLSWRK
160 170 180 190 200
LQLSRAKLKA SSKTSALLSG FAMVAMVEVQ LDHDTNVPPG MLIAFAICTT
210 220 230 240 250
LLVAVHMLAL MISTCILPNI ETVCNLHSIS LVHESPHERL HWYIETAWAF
260 270 280 290 300
STLLGLILFL LEIAILCWVK FYDLSPPAAW SACVVLIPVM IIFMAFAIHF
310 320 330 340 350
YRSLVSHKYE VTVSGIRELE MLKEQMEQDH LEHHNNIRNN GMNYGASGDI

V
Length:351
Mass (Da):38,507
Last modified:May 1, 2000 - v1
Checksum:i328A586D251D3335
GO
Isoform EImported (identifier: Q9U6B8-3) [UniParc]FASTAAdd to basket
Also known as: GImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: MSVWTTANNS...PPPSSQNQAK → MPPFEEGESK...YQQNQSYRFQ

Note: No experimental confirmation available.
Show »
Length:507
Mass (Da):55,693
Checksum:iD810DF0A0AC4ABE0
GO
Isoform CCurated (identifier: Q9U6B8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-132: PRGHHRTASSS → AGRTVQIDCRI
     133-351: Missing.

Note: No experimental confirmation available.
Show »
Length:132
Mass (Da):13,927
Checksum:iE98C39548A114A5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28A → S in ABF54966 (PubMed:16751269).Curated1
Sequence conflicti75H → HPH in ABF54966 (PubMed:16751269).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0183131 – 121MSVWT…QNQAK → MPPFEEGESKPEEQIPLKPP RRHKKMKSADQEAAQTPAED HEEEQLLPGSGTSNLRYARA NLSQSSLMLSHQQGSFESST ERTASSETLDVMPISRRYQV HPQPNRLGIRQPASALASAL HATARLAASVDAYTAAATAT AATGDYGDYMRPQPNLGHAH QLPLTQTTQTAQPLHHQLPA HQLGNLRASNFVGSSRYLYH SQFNSNSPQTRRFTAQRDGS PAYAASVAAASAAAAASAVA PIAPLAPLASIASPPFAAQP PPFQLRTYQQNQSYRFQ in isoform E. 1 PublicationAdd BLAST121
Alternative sequenceiVSP_021772122 – 132PRGHHRTASSS → AGRTVQIDCRI in isoform C. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_021773133 – 351Missing in isoform C. 1 PublicationAdd BLAST219

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503470 mRNA. Translation: ABF54966.1.
AF188634 mRNA. Translation: AAF01457.1.
AE013599 Genomic DNA. Translation: AAF57780.2.
AE013599 Genomic DNA. Translation: AAS64814.2.
AE013599 Genomic DNA. Translation: ACL83150.1.
AE013599 Genomic DNA. Translation: AGB93595.1.
AY071273 mRNA. Translation: AAL48895.1.
BT001876 mRNA. Translation: AAN71648.1.
BT003591 mRNA. Translation: AAO39594.1.
RefSeqiNP_001137696.1. NM_001144224.2. [Q9U6B8-3]
NP_001261063.1. NM_001274134.1. [Q9U6B8-3]
NP_611273.1. NM_137429.3. [Q9U6B8-1]
NP_725727.1. NM_170626.2. [Q9U6B8-1]
NP_995881.2. NM_206159.2. [Q9U6B8-1]
UniGeneiDm.7627.

Genome annotation databases

EnsemblMetazoaiFBtr0086795; FBpp0085974; FBgn0041585. [Q9U6B8-1]
FBtr0086797; FBpp0085976; FBgn0041585. [Q9U6B8-1]
FBtr0114614; FBpp0113106; FBgn0041585. [Q9U6B8-3]
FBtr0336698; FBpp0307679; FBgn0041585. [Q9U6B8-1]
FBtr0336699; FBpp0307680; FBgn0041585. [Q9U6B8-3]
GeneIDi37040.
KEGGidme:Dmel_CG11430.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCRCM1_DROME
AccessioniPrimary (citable) accession number: Q9U6B8
Secondary accession number(s): B7YZJ7
, Q0E937, Q1HCN1, Q7JQY5, Q7KRH6, Q8IGA6, Q9V892
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families