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Protein

Calcium release-activated calcium channel protein 1

Gene

olf186-F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores. Regulates transcription factor NFAT nuclear import.2 Publications

GO - Molecular functioni

  • store-operated calcium channel activity Source: UniProtKB

GO - Biological processi

  • nervous system development Source: FlyBase
  • positive regulation of calcium ion transport Source: UniProtKB
  • positive regulation of NFAT protein import into nucleus Source: UniProtKB
  • regulation of cardiac conduction Source: Reactome
  • store-operated calcium entry Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-DME-139853. Elevation of cytosolic Ca2+ levels.
R-DME-5578775. Ion homeostasis.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Protein family/group databases

TCDBi1.A.52.1.5. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium release-activated calcium channel protein 1
Alternative name(s):
Protein orai
dOrai
Gene namesi
Name:olf186-F
Synonyms:CRACM1, olf186
ORF Names:CG11430
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0041585. olf186-F.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 163163CytoplasmicSequence analysisAdd
BLAST
Transmembranei164 – 18118HelicalSequence analysisAdd
BLAST
Topological domaini182 – 19110ExtracellularSequence analysis
Transmembranei192 – 21221HelicalSequence analysisAdd
BLAST
Topological domaini213 – 24836CytoplasmicSequence analysisAdd
BLAST
Transmembranei249 – 26921HelicalSequence analysisAdd
BLAST
Topological domaini270 – 2778ExtracellularSequence analysis
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 35153CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: GO_Central
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Calcium release-activated calcium channel protein 1PRO_0000234388Add
BLAST

Proteomic databases

PaxDbiQ9U6B8.
PRIDEiQ9U6B8.

Expressioni

Gene expression databases

BgeeiQ9U6B8.
ExpressionAtlasiQ9U6B8. differential.
GenevisibleiQ9U6B8. DM.

Interactioni

Protein-protein interaction databases

BioGridi62725. 1 interaction.
DIPiDIP-59770N.
MINTiMINT-1600663.
STRINGi7227.FBpp0113106.

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi145 – 17935Combined sources
Helixi192 – 21524Combined sources
Turni216 – 2183Combined sources
Helixi237 – 27135Combined sources
Turni272 – 2743Combined sources
Helixi276 – 28510Combined sources
Helixi287 – 30620Combined sources
Helixi308 – 33124Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HKRX-ray3.35A/B133-341[»]
4HKSX-ray3.35A/B133-341[»]
ProteinModelPortaliQ9U6B8.
SMRiQ9U6B8. Positions 144-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi109 – 1146Poly-Pro

Sequence similaritiesi

Belongs to the Orai family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
GeneTreeiENSGT00390000015354.
InParanoidiQ9U6B8.
KOiK16056.
OMAiSQNQAKP.
OrthoDBiEOG7G1V73.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 1 hit.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AImported (identifier: Q9U6B8-1) [UniParc]FASTAAdd to basket

Also known as: BImported

, FImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVWTTANNS GLETPTKSPI TSSVPRAARS SAVITTGNHQ QHHFQHVVAA
60 70 80 90 100
AVAAATSVAT GHQFQQQFPL HAHPHPQHHS NSPTGSGSNS NNSAGFQRTS
110 120 130 140 150
ISNSLLQFPP PPPPSSQNQA KPRGHHRTAS SSMSQSGEDL HSPTYLSWRK
160 170 180 190 200
LQLSRAKLKA SSKTSALLSG FAMVAMVEVQ LDHDTNVPPG MLIAFAICTT
210 220 230 240 250
LLVAVHMLAL MISTCILPNI ETVCNLHSIS LVHESPHERL HWYIETAWAF
260 270 280 290 300
STLLGLILFL LEIAILCWVK FYDLSPPAAW SACVVLIPVM IIFMAFAIHF
310 320 330 340 350
YRSLVSHKYE VTVSGIRELE MLKEQMEQDH LEHHNNIRNN GMNYGASGDI

V
Length:351
Mass (Da):38,507
Last modified:May 1, 2000 - v1
Checksum:i328A586D251D3335
GO
Isoform EImported (identifier: Q9U6B8-3) [UniParc]FASTAAdd to basket

Also known as: GImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: MSVWTTANNS...PPPSSQNQAK → MPPFEEGESK...YQQNQSYRFQ

Note: No experimental confirmation available.
Show »
Length:507
Mass (Da):55,693
Checksum:iD810DF0A0AC4ABE0
GO
Isoform CCurated (identifier: Q9U6B8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-132: PRGHHRTASSS → AGRTVQIDCRI
     133-351: Missing.

Note: No experimental confirmation available.
Show »
Length:132
Mass (Da):13,927
Checksum:iE98C39548A114A5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281A → S in ABF54966 (PubMed:16751269).Curated
Sequence conflicti75 – 751H → HPH in ABF54966 (PubMed:16751269).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 121121MSVWT…QNQAK → MPPFEEGESKPEEQIPLKPP RRHKKMKSADQEAAQTPAED HEEEQLLPGSGTSNLRYARA NLSQSSLMLSHQQGSFESST ERTASSETLDVMPISRRYQV HPQPNRLGIRQPASALASAL HATARLAASVDAYTAAATAT AATGDYGDYMRPQPNLGHAH QLPLTQTTQTAQPLHHQLPA HQLGNLRASNFVGSSRYLYH SQFNSNSPQTRRFTAQRDGS PAYAASVAAASAAAAASAVA PIAPLAPLASIASPPFAAQP PPFQLRTYQQNQSYRFQ in isoform E. 1 PublicationVSP_018313Add
BLAST
Alternative sequencei122 – 13211PRGHHRTASSS → AGRTVQIDCRI in isoform C. 1 PublicationVSP_021772Add
BLAST
Alternative sequencei133 – 351219Missing in isoform C. 1 PublicationVSP_021773Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503470 mRNA. Translation: ABF54966.1.
AF188634 mRNA. Translation: AAF01457.1.
AE013599 Genomic DNA. Translation: AAF57780.2.
AE013599 Genomic DNA. Translation: AAS64814.2.
AE013599 Genomic DNA. Translation: ACL83150.1.
AE013599 Genomic DNA. Translation: AGB93595.1.
AY071273 mRNA. Translation: AAL48895.1.
BT001876 mRNA. Translation: AAN71648.1.
BT003591 mRNA. Translation: AAO39594.1.
RefSeqiNP_001137696.1. NM_001144224.2. [Q9U6B8-3]
NP_001261063.1. NM_001274134.1. [Q9U6B8-3]
NP_611273.1. NM_137429.3. [Q9U6B8-1]
NP_725727.1. NM_170626.2. [Q9U6B8-1]
NP_995881.2. NM_206159.2. [Q9U6B8-1]
UniGeneiDm.7627.

Genome annotation databases

EnsemblMetazoaiFBtr0086795; FBpp0085974; FBgn0041585. [Q9U6B8-1]
FBtr0086797; FBpp0085976; FBgn0041585. [Q9U6B8-1]
FBtr0114614; FBpp0113106; FBgn0041585. [Q9U6B8-3]
FBtr0336698; FBpp0307679; FBgn0041585. [Q9U6B8-1]
FBtr0336699; FBpp0307680; FBgn0041585. [Q9U6B8-3]
GeneIDi37040.
KEGGidme:Dmel_CG11430.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ503470 mRNA. Translation: ABF54966.1.
AF188634 mRNA. Translation: AAF01457.1.
AE013599 Genomic DNA. Translation: AAF57780.2.
AE013599 Genomic DNA. Translation: AAS64814.2.
AE013599 Genomic DNA. Translation: ACL83150.1.
AE013599 Genomic DNA. Translation: AGB93595.1.
AY071273 mRNA. Translation: AAL48895.1.
BT001876 mRNA. Translation: AAN71648.1.
BT003591 mRNA. Translation: AAO39594.1.
RefSeqiNP_001137696.1. NM_001144224.2. [Q9U6B8-3]
NP_001261063.1. NM_001274134.1. [Q9U6B8-3]
NP_611273.1. NM_137429.3. [Q9U6B8-1]
NP_725727.1. NM_170626.2. [Q9U6B8-1]
NP_995881.2. NM_206159.2. [Q9U6B8-1]
UniGeneiDm.7627.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HKRX-ray3.35A/B133-341[»]
4HKSX-ray3.35A/B133-341[»]
ProteinModelPortaliQ9U6B8.
SMRiQ9U6B8. Positions 144-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62725. 1 interaction.
DIPiDIP-59770N.
MINTiMINT-1600663.
STRINGi7227.FBpp0113106.

Protein family/group databases

TCDBi1.A.52.1.5. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

Proteomic databases

PaxDbiQ9U6B8.
PRIDEiQ9U6B8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086795; FBpp0085974; FBgn0041585. [Q9U6B8-1]
FBtr0086797; FBpp0085976; FBgn0041585. [Q9U6B8-1]
FBtr0114614; FBpp0113106; FBgn0041585. [Q9U6B8-3]
FBtr0336698; FBpp0307679; FBgn0041585. [Q9U6B8-1]
FBtr0336699; FBpp0307680; FBgn0041585. [Q9U6B8-3]
GeneIDi37040.
KEGGidme:Dmel_CG11430.

Organism-specific databases

CTDi37040.
FlyBaseiFBgn0041585. olf186-F.

Phylogenomic databases

eggNOGiKOG4298. Eukaryota.
ENOG4110DSB. LUCA.
GeneTreeiENSGT00390000015354.
InParanoidiQ9U6B8.
KOiK16056.
OMAiSQNQAKP.
OrthoDBiEOG7G1V73.

Enzyme and pathway databases

ReactomeiR-DME-139853. Elevation of cytosolic Ca2+ levels.
R-DME-5578775. Ion homeostasis.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

GenomeRNAii37040.
PROiQ9U6B8.

Gene expression databases

BgeeiQ9U6B8.
ExpressionAtlasiQ9U6B8. differential.
GenevisibleiQ9U6B8. DM.

Family and domain databases

InterProiIPR012446. CRAC_channel.
[Graphical view]
PANTHERiPTHR31501. PTHR31501. 1 hit.
PfamiPF07856. Orai-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome-wide RNAi screen of Ca2+ influx identifies genes that regulate Ca2+ release-activated Ca2+ channel activity."
    Zhang S.L., Yeromin A.V., Zhang X.H.-F., Yu Y., Safrina O., Penna A., Roos J., Stauderman K.A., Cahalan M.D.
    Proc. Natl. Acad. Sci. U.S.A. 103:9357-9362(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Tissue: Embryo.
  2. Chodagam S., Tickoo S.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Canton-S.
    Tissue: Head.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND E).
    Strain: Berkeley.
    Tissue: Embryo.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
    Tissue: Embryo.
  7. "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function."
    Feske S., Gwack Y., Prakriya M., Srikanth S., Puppel S.-H., Tanasa B., Hogan P.G., Lewis R.S., Daly M., Rao A.
    Nature 441:179-185(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry."
    Vig M., Peinelt C., Beck A., Koomoa D.L., Rabah D., Koblan-Huberson M., Kraft S., Turner H., Fleig A., Penner R., Kinet J.-P.
    Science 312:1220-1223(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCRCM1_DROME
AccessioniPrimary (citable) accession number: Q9U6B8
Secondary accession number(s): B7YZJ7
, Q0E937, Q1HCN1, Q7JQY5, Q7KRH6, Q8IGA6, Q9V892
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

In Greek mythology, the 'Orai' are the keepers of the gates of heaven: Eunomia (order or harmony), Dike (justice) and Eirene (peace).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.