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Q9U616

- GCSH_DROME

UniProt

Q9U616 - GCSH_DROME

Protein

Glycine cleavage system H protein, mitochondrial

Gene

ppl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity.By similarity

    Cofactori

    Binds 1 lipoyl cofactor covalently.By similarity

    GO - Biological processi

    1. glycine decarboxylation via glycine cleavage system Source: InterPro
    2. wing disc development Source: FlyBase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine cleavage system H protein, mitochondrial
    Alternative name(s):
    Protein pumpless
    Gene namesi
    Name:ppl
    ORF Names:CG7758
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0027945. ppl.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. glycine cleavage complex Source: InterPro
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 165Glycine cleavage system H protein, mitochondrialPRO_0000010728
    Transit peptidei1 – ?MitochondrionBy similarity

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei98 – 981N6-lipoyllysineBy similarity

    Proteomic databases

    PaxDbiQ9U616.
    PRIDEiQ9U616.

    Expressioni

    Gene expression databases

    BgeeiQ9U616.

    Interactioni

    Subunit structurei

    The glycine cleavage system is composed of four proteins: P, T, L and H.By similarity

    Protein-protein interaction databases

    BioGridi65597. 1 interaction.
    DIPiDIP-19395N.
    IntActiQ9U616. 4 interactions.
    MINTiMINT-1578911.
    STRINGi7227.FBpp0078066.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9U616.
    SMRiQ9U616. Positions 42-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvH family.Curated
    Contains 1 lipoyl-binding domain.Curated

    Keywords - Domaini

    Lipoyl, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0509.
    GeneTreeiENSGT00390000011666.
    InParanoidiQ9U616.
    KOiK02437.
    OMAiSSCYEKG.
    OrthoDBiEOG7RNK1M.
    PhylomeDBiQ9U616.

    Family and domain databases

    HAMAPiMF_00272. GcvH.
    InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR002930. GCV_H.
    IPR017453. GCV_H_sub.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PANTHERiPTHR11715. PTHR11715. 1 hit.
    PfamiPF01597. GCV_H. 1 hit.
    [Graphical view]
    SUPFAMiSSF51230. SSF51230. 1 hit.
    TIGRFAMsiTIGR00527. gcvH. 1 hit.
    PROSITEiPS00189. LIPOYL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9U616-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVFITKFARI GLQAARQLSV TPLGAVQARA IHLTSLLAKE RRYTNKHEWV    50
    EVVSGSNAIV GISSYAQEAL GDVVFAQLPE PGTELKQDDE CGALESVKAA 100
    SEVYSPVSGK VIEKNAEVED TPALVNSSCY EKGWLFKVDL KNPKELEALM 150
    TEDQYKAFLS SSGDH 165
    Length:165
    Mass (Da):18,002
    Last modified:May 1, 2000 - v1
    Checksum:iCCEBD650304D359E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF203725 mRNA. Translation: AAF13277.1.
    AE014296 Genomic DNA. Translation: AAF51697.3.
    AY075433 mRNA. Translation: AAL68248.1.
    RefSeqiNP_524197.1. NM_079473.4.
    UniGeneiDm.29757.

    Genome annotation databases

    EnsemblMetazoaiFBtr0078412; FBpp0078066; FBgn0027945.
    GeneIDi40349.
    KEGGidme:Dmel_CG7758.
    UCSCiCG7758-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF203725 mRNA. Translation: AAF13277.1 .
    AE014296 Genomic DNA. Translation: AAF51697.3 .
    AY075433 mRNA. Translation: AAL68248.1 .
    RefSeqi NP_524197.1. NM_079473.4.
    UniGenei Dm.29757.

    3D structure databases

    ProteinModelPortali Q9U616.
    SMRi Q9U616. Positions 42-158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 65597. 1 interaction.
    DIPi DIP-19395N.
    IntActi Q9U616. 4 interactions.
    MINTi MINT-1578911.
    STRINGi 7227.FBpp0078066.

    Proteomic databases

    PaxDbi Q9U616.
    PRIDEi Q9U616.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0078412 ; FBpp0078066 ; FBgn0027945 .
    GeneIDi 40349.
    KEGGi dme:Dmel_CG7758.
    UCSCi CG7758-RA. d. melanogaster.

    Organism-specific databases

    CTDi 5493.
    FlyBasei FBgn0027945. ppl.

    Phylogenomic databases

    eggNOGi COG0509.
    GeneTreei ENSGT00390000011666.
    InParanoidi Q9U616.
    KOi K02437.
    OMAi SSCYEKG.
    OrthoDBi EOG7RNK1M.
    PhylomeDBi Q9U616.

    Miscellaneous databases

    GenomeRNAii 40349.
    NextBioi 818315.
    PROi Q9U616.

    Gene expression databases

    Bgeei Q9U616.

    Family and domain databases

    HAMAPi MF_00272. GcvH.
    InterProi IPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR002930. GCV_H.
    IPR017453. GCV_H_sub.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    PANTHERi PTHR11715. PTHR11715. 1 hit.
    Pfami PF01597. GCV_H. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51230. SSF51230. 1 hit.
    TIGRFAMsi TIGR00527. gcvH. 1 hit.
    PROSITEi PS00189. LIPOYL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of food intake and growth by amino acids in Drosophila: the role of pumpless, a fat body expressed gene with homology to vertebrate glycine cleavage system."
      Zinke I., Kirchner C., Chao L.C., Tetzlaff M.T., Pankratz M.J.
      Development 126:5275-5284(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Larva and Pupae.

    Entry informationi

    Entry nameiGCSH_DROME
    AccessioniPrimary (citable) accession number: Q9U616
    Secondary accession number(s): Q9VP59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3