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Protein

Glycine cleavage system H protein, mitochondrial

Gene

ppl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).By similarity

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

GO - Biological processi

  1. glycine decarboxylation via glycine cleavage system Source: InterPro
  2. wing disc development Source: FlyBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein, mitochondrial
Alternative name(s):
Protein pumpless
Gene namesi
Name:ppl
ORF Names:CG7758
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0027945. ppl.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. glycine cleavage complex Source: InterPro
  2. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 165Glycine cleavage system H protein, mitochondrialPRO_0000010728
Transit peptidei1 – ?MitochondrionBy similarity

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981N6-lipoyllysineBy similarityPROSITE-ProRule annotation

Proteomic databases

PaxDbiQ9U616.
PRIDEiQ9U616.

Expressioni

Gene expression databases

BgeeiQ9U616.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.By similarity

Protein-protein interaction databases

BioGridi65597. 1 interaction.
DIPiDIP-19395N.
IntActiQ9U616. 4 interactions.
MINTiMINT-1578911.
STRINGi7227.FBpp0078066.

Structurei

3D structure databases

ProteinModelPortaliQ9U616.
SMRiQ9U616. Positions 42-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 13983Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GcvH family.Curated
Contains 1 lipoyl-binding domain.CuratedPROSITE-ProRule annotation

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiCOG0509.
GeneTreeiENSGT00390000011666.
InParanoidiQ9U616.
KOiK02437.
OMAiCYAEGWL.
OrthoDBiEOG7RNK1M.
PhylomeDBiQ9U616.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9U616-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVFITKFARI GLQAARQLSV TPLGAVQARA IHLTSLLAKE RRYTNKHEWV
60 70 80 90 100
EVVSGSNAIV GISSYAQEAL GDVVFAQLPE PGTELKQDDE CGALESVKAA
110 120 130 140 150
SEVYSPVSGK VIEKNAEVED TPALVNSSCY EKGWLFKVDL KNPKELEALM
160
TEDQYKAFLS SSGDH
Length:165
Mass (Da):18,002
Last modified:May 1, 2000 - v1
Checksum:iCCEBD650304D359E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203725 mRNA. Translation: AAF13277.1.
AE014296 Genomic DNA. Translation: AAF51697.3.
AY075433 mRNA. Translation: AAL68248.1.
RefSeqiNP_524197.1. NM_079473.4.
UniGeneiDm.29757.

Genome annotation databases

EnsemblMetazoaiFBtr0078412; FBpp0078066; FBgn0027945.
GeneIDi40349.
KEGGidme:Dmel_CG7758.
UCSCiCG7758-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203725 mRNA. Translation: AAF13277.1.
AE014296 Genomic DNA. Translation: AAF51697.3.
AY075433 mRNA. Translation: AAL68248.1.
RefSeqiNP_524197.1. NM_079473.4.
UniGeneiDm.29757.

3D structure databases

ProteinModelPortaliQ9U616.
SMRiQ9U616. Positions 42-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65597. 1 interaction.
DIPiDIP-19395N.
IntActiQ9U616. 4 interactions.
MINTiMINT-1578911.
STRINGi7227.FBpp0078066.

Proteomic databases

PaxDbiQ9U616.
PRIDEiQ9U616.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078412; FBpp0078066; FBgn0027945.
GeneIDi40349.
KEGGidme:Dmel_CG7758.
UCSCiCG7758-RA. d. melanogaster.

Organism-specific databases

CTDi5493.
FlyBaseiFBgn0027945. ppl.

Phylogenomic databases

eggNOGiCOG0509.
GeneTreeiENSGT00390000011666.
InParanoidiQ9U616.
KOiK02437.
OMAiCYAEGWL.
OrthoDBiEOG7RNK1M.
PhylomeDBiQ9U616.

Miscellaneous databases

GenomeRNAii40349.
NextBioi818315.
PROiQ9U616.

Gene expression databases

BgeeiQ9U616.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of food intake and growth by amino acids in Drosophila: the role of pumpless, a fat body expressed gene with homology to vertebrate glycine cleavage system."
    Zinke I., Kirchner C., Chao L.C., Tetzlaff M.T., Pankratz M.J.
    Development 126:5275-5284(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Larva and Pupae.

Entry informationi

Entry nameiGCSH_DROME
AccessioniPrimary (citable) accession number: Q9U616
Secondary accession number(s): Q9VP59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.