Q9U616 (GCSH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine cleavage system H protein, mitochondrial

Alternative name(s):
Protein pumpless

Gene names

Name:	ppl
ORF Names:	CG7758

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity.
Cofactor	Binds 1 lipoyl cofactor covalently By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the GcvH family. Contains 1 lipoyl-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Lipoyl Transit peptide
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: InterPro wing disc development Inferred from genetic interaction PubMed 20730086. Source: FlyBase
Cellular_component	glycine cleavage complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion By similarity
	Chain	? – 165		Glycine cleavage system H protein, mitochondrial		PRO_0000010728
Amino acid modifications
	Modified residue	98	1	N6-lipoyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9U616 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CCEBD650304D359E	FASTA	165	18,002
	10 20 30 40 50 60 MVFITKFARI GLQAARQLSV TPLGAVQARA IHLTSLLAKE RRYTNKHEWV EVVSGSNAIV 70 80 90 100 110 120 GISSYAQEAL GDVVFAQLPE PGTELKQDDE CGALESVKAA SEVYSPVSGK VIEKNAEVED 130 140 150 160 TPALVNSSCY EKGWLFKVDL KNPKELEALM TEDQYKAFLS SSGDH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of food intake and growth by amino acids in Drosophila: the role of pumpless, a fat body expressed gene with homology to vertebrate glycine cleavage system." Zinke I., Kirchner C., Chao L.C., Tetzlaff M.T., Pankratz M.J. Development 126:5275-5284(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Larva and Pupae.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF203725 mRNA. Translation: AAF13277.1. AE014296 Genomic DNA. Translation: AAF51697.3. AY075433 mRNA. Translation: AAL68248.1.
RefSeq	NP_524197.1. NM_079473.4.
UniGene	Dm.29757.
3D structure databases
ProteinModelPortal	Q9U616.
SMR	Q9U616. Positions 42-158.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19395N.
IntAct	Q9U616. 4 interactions.
MINT	MINT-1578911.
STRING	7227.FBpp0078066.
Proteomic databases
PaxDb	Q9U616.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0078412; FBpp0078066; FBgn0027945.
GeneID	40349.
KEGG	dme:Dmel_CG7758.
UCSC	CG7758-RA. d. melanogaster.
Organism-specific databases
CTD	5493.
FlyBase	FBgn0027945. ppl.
Phylogenomic databases
eggNOG	COG0509.
GeneTree	ENSGT00390000011666.
InParanoid	Q9U616.
KO	K02437.
OMA	TESHEWI.
OrthoDB	EOG4PVMG8.
PhylomeDB	Q9U616.
Gene expression databases
Bgee	Q9U616.
GermOnline	CG7758. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR002930. GCV_H. IPR017453. GCV_H_sub. IPR011053. Single_hybrid_motif. [Graphical view]
PANTHER	PTHR11715. PTHR11715. 1 hit.
Pfam	PF01597. GCV_H. 1 hit. [Graphical view]
SUPFAM	SSF51230. Hybrid_motif. 1 hit.
TIGRFAMs	TIGR00527. gcvH. 1 hit.
PROSITE	PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	40349.
NextBio	818315.

Entry information

Entry name

GCSH_DROME

Accession

Primary (citable) accession number: Q9U616
Secondary accession number(s): Q9VP59

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project