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Protein

Enolase

Gene

ENOL

Organism
Mastigamoeba balamuthi (Phreatamoeba balamuthi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPD)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENOL)
  5. Pyruvate kinase (pk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157SubstrateBy similarity1
Binding sitei166SubstrateBy similarity1
Active sitei209Proton donorBy similarity1
Metal bindingi244MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi324MagnesiumBy similarity1
Binding sitei324SubstrateBy similarity1
Active sitei349Proton acceptorBy similarity1
Binding sitei400SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENOL
OrganismiMastigamoeba balamuthi (Phreatamoeba balamuthi)
Taxonomic identifieri108607 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeMastigamoebidaeMastigamoeba

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340871 – 439EnolaseAdd BLAST439

Proteomic databases

PRIDEiQ9U615.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9U615.
SMRiQ9U615.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni376 – 379Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9U615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKSVFAR EILDSRGNPT VEVDLTTEKG LFRSAVPSGA STGIYEACEL
60 70 80 90 100
RDGDKSRYLG KGVLKAVENV NKILAPKLIG LDVTKQGEID RLMLQIDGTE
110 120 130 140 150
NKTHLGANAI LGCSMSVCRA AAAFRGLPLY RYIAELSGNK SPMLPLPCFN
160 170 180 190 200
VINGGEHAGN KLAMQEFMIC PTGATSFHEA LRMAAETYHN LKLVIKKRYG
210 220 230 240 250
MDATNVGDEG GFAPNIQANH EGLELIVEAI KQAGYTGKIE IGMDVAASSF
260 270 280 290 300
WDAKESKYDL GFKVPADKKT PDMLVSGEGL IKLYEEWTSK YPIWSIEDPF
310 320 330 340 350
DQDDWATYTR FTELIRNRIQ IVGDDLLVTN PKRIVEARNK KACNALLLKL
360 370 380 390 400
NQIGSVSEAV EACRLAREVN WGVMVSHRSG ETEDAFIADL VVGLGCGQIK
410 420 430
TGAPCRSERL AKYNQLLRIE EELGANAHYA AKTLSGVGH
Length:439
Mass (Da):48,006
Last modified:May 1, 2000 - v1
Checksum:iF2CEDE165BBC31F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205070 mRNA. Translation: AAF13454.1.
AF348478 Genomic DNA. Translation: AAK31161.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_MASBA
AccessioniPrimary (citable) accession number: Q9U615
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: May 1, 2000
Last modified: April 12, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families