Reviewed, UniProtKB/Swiss-Prot Q9U3X4 (DHSA_DICDI)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Flavoprotein subunit of complex II
      Short name=FP

Gene names

Name:	sdhA
ORF Names:	DDB_G0280535

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Potential
	Chain	25 – 626	602	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial		PRO_0000327828
Regions
	Nucleotide binding	69 – 84	16	FAD By similarity
Sites
	Active site	318	1	Proton acceptor By similarity
	Binding site	274	1	Substrate By similarity
	Binding site	286	1	Substrate By similarity
	Binding site	385	1	Substrate By similarity
	Binding site	429	1	Substrate By similarity
Amino acid modifications
	Modified residue	77	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9U3X4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C8F27868BD063D67	FASTA	626	68,516
	10 20 30 40 50 60 MLSSALKLTK KVCSTKSNGL IRSFSTQTQS RDYAVVDHTY DAIVVGAGGA GLRAALGLTE 70 80 90 100 110 120 KGYKTACITK LFPTRSHTVA AQGGINAALG NADQDDWRWH AYDTVKGSDF LGDQDAIHYM 130 140 150 160 170 180 CKEAVPTVLE LEQYGVPFSR MDDGRIYQRA FGGQSKNFGK GGQATRCCAA ADRTGHALLH 190 200 210 220 230 240 TLYGQAVKHN TKFFIEYFVT DLIMENGDCR GVVAINLEDG TIHRFRSHAT VIATGGYGRA 250 260 270 280 290 300 YFSATSAHTC TGDGNAMVIR AGLPCQDLEF VQFHPTGIYG SGCLITEGAR GEGGYLLNSS 310 320 330 340 350 360 GERFMPRYAP SVADLASRDV VSRSETMEIR EGRGVGPEKD HCLLNLTHLS PEIIDERLPG 370 380 390 400 410 420 IRETAMIFAG VDVTKEPIPV IPTVHYNMGG IPTNYKGQVI TQVDGKDKLV KGLYAAGESA 430 440 450 460 470 480 CVSVHGANRL GANSLLDIVV FGRAVANEIE NTLAKDTPHK PLPPNAGEES IANIDAIRFS 490 500 510 520 530 540 NGTRSTAEIR LEMQKIMQRN AAVFRDGQVL KEGVELIDKC ARSLINDLKT TDRTMIWNTD 550 560 570 580 590 600 LIESLELQNL MTQAVLTMHS AEARKESRGA HAREDYKERD DANWMKHTLS YLDVNTGKVT 610 620 LNYRPVVSET LDQSEMETIK PFKRVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The flavoprotein subunit 1 of Dictyostelium succinate dehydrogenase." Lay S.P., Fisher P.R. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF211482 Genomic DNA. Translation: AAF21045.1. AAFI02000037 Genomic DNA. Translation: EAL67069.1.
RefSeq	XP_641069.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
ModBase	Search...
Genome annotation databases
GeneID	3392159.
KEGG	ddi:DDB_0214886.
Organism-specific databases
dictyBase	DDB_G0280535. sdhA.
Phylogenomic databases
OMA	Q9U3X4. EEDDWRW.
Enzyme and pathway databases
BRENDA	1.3.5.1. 424.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_DICDI

Accession

Primary (citable) accession number: Q9U3X4
Secondary accession number(s): Q54V62

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)