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Protein

Glycogen synthase kinase-3

Gene

gsk-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates oma-1, a regulator of the oocyte-to-embryo transition, enabling its degradation. Phosphorylates skn-1, preventing it from accumulating in nuclei and thus inhibiting phase II gene expression in the oxidative stress defense. Involved in mesendoderm specification and mitotic spindle orientation in EMS blastomeres. Thought to be a branch point in these processes as proteins downstream are not required. Negatively regulates Wnt signaling in vulval precursor cells and acts as a Wnt-independent repressor of med-1 and med-2 in the C lineage inhibiting mesoderm development. Required for normal lifespan and LiCl-induced lifespan extension.8 Publications

Catalytic activityi

ATP + [tau protein] = ADP + [tau protein] phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: WormBase
  • tau-protein kinase activity Source: UniProtKB-EC

GO - Biological processi

  • engulfment of apoptotic cell Source: WormBase
  • establishment of mitotic spindle orientation Source: UniProtKB
  • germ cell development Source: WormBase
  • left/right axis specification Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • peptidyl-threonine phosphorylation Source: WormBase
  • positive regulation of distal tip cell migration Source: UniProtKB
  • positive regulation of engulfment of apoptotic cell Source: UniProtKB
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: WormBase
  • Wnt signaling pathway, regulating spindle positioning Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-3371453. Regulation of HSF1-mediated heat shock response.
SignaLinkiQ9U2Q9.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen synthase kinase-31 Publication (EC:2.7.11.26)
Gene namesi
Name:gsk-3Imported
Synonyms:sgg-11 Publication
ORF Names:Y18D10A.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiY18D10A.5; CE21401; WBGene00001746; gsk-3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Worms exhibit defects in endoderm specification and mitotic spindle alignment. Mutants show reduced degradation of oma-1 and have shortened lifespan.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003491361 – 362Glycogen synthase kinase-3Add BLAST362

Proteomic databases

EPDiQ9U2Q9.
PaxDbiQ9U2Q9.
PeptideAtlasiQ9U2Q9.
PRIDEiQ9U2Q9.

Expressioni

Gene expression databases

BgeeiWBGene00001746.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with axl-1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
axl-1Q3LRZ34EBI-330089,EBI-327368
mac-1Q9NAG43EBI-330089,EBI-2005767
pry-1O620906EBI-330089,EBI-2917690

Protein-protein interaction databases

BioGridi38546. 22 interactors.
DIPiDIP-25216N.
IntActiQ9U2Q9. 13 interactors.
MINTiMINT-1073589.
STRINGi6239.Y18D10A.5.2.

Structurei

3D structure databases

ProteinModelPortaliQ9U2Q9.
SMRiQ9U2Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 320Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0658. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00520000055635.
HOGENOMiHOG000233017.
InParanoidiQ9U2Q9.
KOiK03083.
OMAiCFFDELR.
OrthoDBiEOG091G099S.
PhylomeDBiQ9U2Q9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9U2Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKQLLSCSL KSGKQVTMVV ASVATDGVDQ QVEISYYDQK VIGNGSFGVV
60 70 80 90 100
FLAKLSTTNE MVAIKKVLQD KRFKNRELQI MRKLNHPNIV KLKYFFYSSG
110 120 130 140 150
EKKDELYLNL ILEYVPETVY RVARHYSKQR QQIPMIYVKL YMYQLLRSLA
160 170 180 190 200
YIHSIGICHR DIKPQNLLID PESGVLKLCD FGSAKYLVRN EPNVSYICSR
210 220 230 240 250
YYRAPELIFG ATNYTNSIDV WSAGTVMAEL LLGQPIFPGD SGVDQLVEII
260 270 280 290 300
KVLGTPTREQ IQSMNPNYKE FKFPQIKAHP WNKVFRVHTP AEAIDLISKI
310 320 330 340 350
IEYTPTSRPT PQAACQHAFF DELRNPDARL PSGRPLPTLE MDGPMGTGEV
360
STTSGDVAGP SA
Length:362
Mass (Da):40,882
Last modified:May 1, 2000 - v1
Checksum:iABDD2F49CC475BF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350V → I in AAD45354 (PubMed:10444600).Curated1
Sequence conflicti352T → P in AAD45354 (PubMed:10444600).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159950 mRNA. Translation: AAD45354.1.
AL034393 Genomic DNA. Translation: CAA22311.1.
PIRiT26520.
RefSeqiNP_493243.1. NM_060842.5.
UniGeneiCel.7399.

Genome annotation databases

EnsemblMetazoaiY18D10A.5; Y18D10A.5; WBGene00001746.
GeneIDi173149.
KEGGicel:CELE_Y18D10A.5.
UCSCiY18D10A.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159950 mRNA. Translation: AAD45354.1.
AL034393 Genomic DNA. Translation: CAA22311.1.
PIRiT26520.
RefSeqiNP_493243.1. NM_060842.5.
UniGeneiCel.7399.

3D structure databases

ProteinModelPortaliQ9U2Q9.
SMRiQ9U2Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38546. 22 interactors.
DIPiDIP-25216N.
IntActiQ9U2Q9. 13 interactors.
MINTiMINT-1073589.
STRINGi6239.Y18D10A.5.2.

Proteomic databases

EPDiQ9U2Q9.
PaxDbiQ9U2Q9.
PeptideAtlasiQ9U2Q9.
PRIDEiQ9U2Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY18D10A.5; Y18D10A.5; WBGene00001746.
GeneIDi173149.
KEGGicel:CELE_Y18D10A.5.
UCSCiY18D10A.5. c. elegans.

Organism-specific databases

CTDi173149.
WormBaseiY18D10A.5; CE21401; WBGene00001746; gsk-3.

Phylogenomic databases

eggNOGiKOG0658. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00520000055635.
HOGENOMiHOG000233017.
InParanoidiQ9U2Q9.
KOiK03083.
OMAiCFFDELR.
OrthoDBiEOG091G099S.
PhylomeDBiQ9U2Q9.

Enzyme and pathway databases

ReactomeiR-CEL-3371453. Regulation of HSF1-mediated heat shock response.
SignaLinkiQ9U2Q9.

Miscellaneous databases

PROiQ9U2Q9.

Gene expression databases

BgeeiWBGene00001746.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSK3_CAEEL
AccessioniPrimary (citable) accession number: Q9U2Q9
Secondary accession number(s): Q9Y0C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.