Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9U2M4 (HOT_CAEEL)

Last modified November 3, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24
Gene names
ORF Names: Y38F1A.6
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Hide | Top

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.

Catalytic activity

(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.

2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Hide | Top

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmolecular hydrogen transport

Inferred from sequence or structural similarity. Source: UniProtKB

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functionhydroxyacid-oxoacid transhydrogenase activity

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 465Probable hydroxyacid-oxoacid transhydrogenase, mitochondrialPRO_0000324756

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q9U2M4-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: CB9A08D7376E2949

FASTA46550,506
        10         20         30         40         50         60 
MSASLARGIL SKMGGSCCPH HAPATNPFKL AKLHGNNKST DYAFEMVCST LRFGKGVTLE 

        70         80         90        100        110        120 
IGYDVRNLGA KKTLLITDKN VQNTIAFKNA EQALKMVNIE YEVFDDVLIE PTVNSMQKAI 

       130        140        150        160        170        180 
AFAKSKQFDS FIAVGGGSVI DTTKAAALYA SNPEADFLDF VGPPFGKSMQ PKNPMLPLIA 

       190        200        210        220        230        240 
VPTTAGTGSE TTAAAIMDLP EHKCKTGIRL RCIKPYLAVV DPLNVMSMPR NVAIYSGFDV 

       250        260        270        280        290        300 
LCHALESFTA LPFDQRSPRP ENPGVRPLYQ GSNPISDVWS KEALRIIGKY FRRSIFDPTD 

       310        320        330        340        350        360 
EEARTEMLKA SSFAGIGFGN AGVHLCHGLS YPISSQAKSC VADDYPKEKN LIPHGLSVMT 

       370        380        390        400        410        420 
TAVADFEFTT AACPDRHLIS AQTLGADIPN NASNEYISRT LCDRLRGYMR DFGVPNGLKG 

       430        440        450        460 
MGFEFSDIEM LTEAASHSVP NIAISPKSAD REIISTLYEK SLTVY

« Hide

Hide | Top

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.

Hide | Top

Cross-references

Sequence databases

AL032639 Genomic DNA. Translation: CAA21631.2.
PIRE88343.
T26686.
RefSeqNP_496764.1.
UniGeneCel.8339

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:24327N.
IntActQ9U2M4. 1 interaction.
STRINGQ9U2M4.

Genome annotation databases

EnsemblY38F1A.6.1; Y38F1A.6.1; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.2; Y38F1A.6.2; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.3; Y38F1A.6.3; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.4; Y38F1A.6.4; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.5; Y38F1A.6.5; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.6; Y38F1A.6.6; Y38F1A.6; Caenorhabditis elegans. [Genome view]
Y38F1A.6.7; Y38F1A.6.7; Y38F1A.6; Caenorhabditis elegans. [Genome view]
GeneID174942.
KEGGcel:Y38F1A.6.
UCSCY38F1A.6.1. c. elegans.

Organism-specific databases

CTD174942.
WormBaseWBGene00012608. Y38F1A.6.
WormPepY38F1A.6. CE24222. [WorfDB]

Phylogenomic databases

OMAYSKADIP.

Enzyme and pathway databases

BRENDA1.1.99.24. 672.

Gene expression databases

ArrayExpressQ9U2M4.

Family and domain databases

InterProIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
[Graphical view]
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio886136.

Hide | Top

Entry information

Entry nameHOT_CAEEL
AccessionPrimary (citable) accession number: Q9U2M4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Hide | Top

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents