Q9U2M4 (HOT_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Short name=HOT
EC=1.1.99.24

Gene names

ORF Names:

Y38F1A.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 4-hydroxybutanoate + 2-oxoglutarate = succinic semialdehyde + (R)-2-hydroxyglutarate.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 465		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000324756

Sequences

	Sequence		Length	Mass (Da)
	Q9U2M4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CB9A08D7376E2949	FASTA	465	50,506
	10 20 30 40 50 60 MSASLARGIL SKMGGSCCPH HAPATNPFKL AKLHGNNKST DYAFEMVCST LRFGKGVTLE 70 80 90 100 110 120 IGYDVRNLGA KKTLLITDKN VQNTIAFKNA EQALKMVNIE YEVFDDVLIE PTVNSMQKAI 130 140 150 160 170 180 AFAKSKQFDS FIAVGGGSVI DTTKAAALYA SNPEADFLDF VGPPFGKSMQ PKNPMLPLIA 190 200 210 220 230 240 VPTTAGTGSE TTAAAIMDLP EHKCKTGIRL RCIKPYLAVV DPLNVMSMPR NVAIYSGFDV 250 260 270 280 290 300 LCHALESFTA LPFDQRSPRP ENPGVRPLYQ GSNPISDVWS KEALRIIGKY FRRSIFDPTD 310 320 330 340 350 360 EEARTEMLKA SSFAGIGFGN AGVHLCHGLS YPISSQAKSC VADDYPKEKN LIPHGLSVMT 370 380 390 400 410 420 TAVADFEFTT AACPDRHLIS AQTLGADIPN NASNEYISRT LCDRLRGYMR DFGVPNGLKG 430 440 450 460 MGFEFSDIEM LTEAASHSVP NIAISPKSAD REIISTLYEK SLTVY

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL032639 Genomic DNA. Translation: CAA21631.2.
PIR	E88343. T26686.
RefSeq	NP_496764.1. NM_064363.3.
UniGene	Cel.8339.
3D structure databases
ProteinModelPortal	Q9U2M4.
SMR	Q9U2M4. Positions 42-463.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24327N.
MINT	MINT-1044715.
STRING	Q9U2M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y38F1A.6.1; Y38F1A.6.1; Y38F1A.6. Y38F1A.6.2; Y38F1A.6.2; Y38F1A.6. Y38F1A.6.3; Y38F1A.6.3; Y38F1A.6. Y38F1A.6.4; Y38F1A.6.4; Y38F1A.6. Y38F1A.6.5; Y38F1A.6.5; Y38F1A.6. Y38F1A.6.6; Y38F1A.6.6; Y38F1A.6. Y38F1A.6.7; Y38F1A.6.7; Y38F1A.6.
GeneID	174942.
KEGG	cel:Y38F1A.6.
UCSC	Y38F1A.6.1. c. elegans.
Organism-specific databases
CTD	174942.
WormBase	Y38F1A.6; CE24222; WBGene00012608.
Phylogenomic databases
eggNOG	meNOG05491.
GeneTree	EMGT00050000017002.
HOGENOM	HBG742415.
InParanoid	Q9U2M4.
OMA	YSKADIP.
PhylomeDB	Q9U2M4.
Gene expression databases
ArrayExpress	Q9U2M4.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
KO	K11173.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	886136.

Entry information

Entry name

HOT_CAEEL

Accession

Primary (citable) accession number: Q9U2M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project