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Protein

Glycogen [starch] synthase

Gene

gsy-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).1 Publication

Kineticsi

  1. KM=3.9 mM for UDP-glucose (at 30 degrees Celsius)1 Publication

Vmax=126 pmol/min/µg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561UDP-glucoseBy similarity

GO - Molecular functioni

  1. glycogen (starch) synthase activity Source: GO_Central

GO - Biological processi

  1. glycogen biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

ReactomeiREACT_184645. Glycogen synthesis.
REACT_220774. Myoclonic epilepsy of Lafora.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase (EC:2.4.1.111 Publication)
Short name:
GS1 Publication
Gene namesi
Name:gsy-1Imported
ORF Names:Y46G5A.31Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiY46G5A.31; CE24302; WBGene00001793; gsy-1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi151 – 1511F → A: Partially inhibits the interaction with gyg-1. Loss of function. 1 Publication
Mutagenesisi151 – 1511F → R: Partially inhibits the interaction with gyg-1. Loss of function. 1 Publication
Mutagenesisi157 – 1571G → R: Partially inhibits the interaction with gyg-1. Loss of function. 1 Publication
Mutagenesisi257 – 2571Y → A: Complete inhibition of the interaction with gyg-1. Loss of function. 1 Publication
Mutagenesisi261 – 2611C → A: Slight reduction in the interaction with gyg-1. Partial loss of function in absence of glycogen branching enzyme. 1 Publication
Mutagenesisi261 – 2611C → R: Complete inhibition of the interaction with gyg-1. Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 672672Glycogen [starch] synthasePRO_0000194770Add
BLAST

Proteomic databases

PaxDbiQ9U2D9.
PRIDEiQ9U2D9.

Interactioni

Subunit structurei

Forms a heteroctomer with each protomer of the gsy-1 homotetramer bound to one molecule of gyg-1. The N-terminus is involved in interprotomer contacts with gyg-1. The interaction with gyg-1 is required for glycogen production but is not required for gsy-1 intrinsic activity.1 Publication

Protein-protein interaction databases

BioGridi40222. 10 interactions.
DIPiDIP-26557N.
IntActiQ9U2D9. 14 interactions.
MINTiMINT-1087535.
STRINGi6239.Y46G5A.31.

Structurei

Secondary structure

1
672
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi13 – 219Combined sources
Helixi27 – 293Combined sources
Helixi37 – 426Combined sources
Beta strandi44 – 507Combined sources
Turni51 – 544Combined sources
Beta strandi57 – 593Combined sources
Helixi60 – 7516Combined sources
Helixi76 – 783Combined sources
Beta strandi79 – 846Combined sources
Helixi89 – 924Combined sources
Beta strandi94 – 963Combined sources
Helixi102 – 11110Combined sources
Helixi112 – 1143Combined sources
Beta strandi118 – 1236Combined sources
Beta strandi130 – 1345Combined sources
Helixi136 – 1427Combined sources
Helixi143 – 15311Combined sources
Helixi163 – 18422Combined sources
Beta strandi192 – 1998Combined sources
Turni200 – 2023Combined sources
Helixi203 – 2119Combined sources
Beta strandi215 – 22410Combined sources
Helixi226 – 2338Combined sources
Helixi247 – 2537Combined sources
Helixi257 – 26913Combined sources
Beta strandi270 – 2778Combined sources
Helixi278 – 28710Combined sources
Beta strandi293 – 2953Combined sources
Helixi302 – 3043Combined sources
Turni308 – 3103Combined sources
Helixi311 – 32919Combined sources
Turni330 – 3323Combined sources
Helixi338 – 3403Combined sources
Beta strandi341 – 35010Combined sources
Turni352 – 3565Combined sources
Helixi357 – 37317Combined sources
Turni377 – 3815Combined sources
Beta strandi383 – 3897Combined sources
Beta strandi394 – 3974Combined sources
Helixi399 – 43032Combined sources
Turni431 – 4333Combined sources
Helixi438 – 4403Combined sources
Helixi444 – 45512Combined sources
Beta strandi465 – 4717Combined sources
Helixi476 – 4838Combined sources
Beta strandi493 – 4986Combined sources
Helixi514 – 5207Combined sources
Beta strandi522 – 5254Combined sources
Beta strandi529 – 5335Combined sources
Helixi535 – 5428Combined sources
Beta strandi547 – 5504Combined sources
Helixi554 – 5629Combined sources
Helixi566 – 5694Combined sources
Beta strandi571 – 5744Combined sources
Beta strandi577 – 5793Combined sources
Helixi581 – 59616Combined sources
Helixi600 – 61314Combined sources
Helixi614 – 6174Combined sources
Helixi619 – 6224Combined sources
Helixi624 – 63714Combined sources
Helixi641 – 6477Combined sources
Turni649 – 6513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QLBX-ray2.60A/B/C/D1-672[»]
ProteinModelPortaliQ9U2D9.
SMRiQ9U2D9. Positions 5-654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 3 family.Curated

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
InParanoidiQ9U2D9.
KOiK00693.
OMAiIMKRAIY.
OrthoDBiEOG741Z1N.
PhylomeDBiQ9U2D9.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9U2D9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPDHARMPRN LSSNKIAKTI AGEDLDEEEV LEMDAGQSAR EEGRFVFECA
60 70 80 90 100
WEVANKVGGI YTVLRSKAQI STEELGDQYC MFGPMKDGKW RLEVDPIEPE
110 120 130 140 150
NRTIRAAMKR FQADGFRCMY GRWLIEGYPK VILFDLGSGA VKMNEWKHEL
160 170 180 190 200
FEQCKIGIPH EDIESNDAVI LGFMVALFLK HFRESVTSYT PLVVAHFHEW
210 220 230 240 250
QAGVGLLMTR LWKLDIATVY TTHATLLGRH LCAGGADLYN NLDSFDLDAE
260 270 280 290 300
AGKRKIYHQY CLERAACQTA HIFTTVSEIT GLEAEHFLCR KPDVLTPNGL
310 320 330 340 350
NVVKFAALHE FQNLHAQNKE KINQFIRGHF HGHLDFDLDK TLYFFTAGRY
360 370 380 390 400
EFSNKGGDMF IESLARLNHY LKTTSDPRHM GVTVVAFLIY PAPANSFNVE
410 420 430 440 450
SLKGQAVTKQ LKEAVDRIKE KVGQRIFDIC LQGHLPEPEE LMSPADNILL
460 470 480 490 500
KRCIMSLHNS SLPPICTHNM IRADDPVLES LRRTSLFNKP EDRVKVVFHP
510 520 530 540 550
EFLSSVSPLI GLDYEDFVRG CHLGVFPSYY EPWGYTPAEC TVMGIPSVST
560 570 580 590 600
NLSGFGCFMQ EHVEDHEQKG IYVIDRRHKA AEESVQELAQ VMYDFCGQSR
610 620 630 640 650
RQRIILRNSN EGLSALLDWQ NLGVFYRDCR RLALERLHPD VDKIMRDNEG
660 670
KVPSAATSRR PSIHSSDGED DE
Length:672
Mass (Da):76,459
Last modified:May 1, 2000 - v1
Checksum:i3B3C3E9044CAC8A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL110485 Genomic DNA. Translation: CAB60373.1.
RefSeqiNP_496736.1. NM_064335.5.
UniGeneiCel.7404.

Genome annotation databases

EnsemblMetazoaiY46G5A.31; Y46G5A.31; WBGene00001793.
GeneIDi174924.
KEGGicel:CELE_Y46G5A.31.
UCSCiY46G5A.31. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL110485 Genomic DNA. Translation: CAB60373.1.
RefSeqiNP_496736.1. NM_064335.5.
UniGeneiCel.7404.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QLBX-ray2.60A/B/C/D1-672[»]
ProteinModelPortaliQ9U2D9.
SMRiQ9U2D9. Positions 5-654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40222. 10 interactions.
DIPiDIP-26557N.
IntActiQ9U2D9. 14 interactions.
MINTiMINT-1087535.
STRINGi6239.Y46G5A.31.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Proteomic databases

PaxDbiQ9U2D9.
PRIDEiQ9U2D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY46G5A.31; Y46G5A.31; WBGene00001793.
GeneIDi174924.
KEGGicel:CELE_Y46G5A.31.
UCSCiY46G5A.31. c. elegans.

Organism-specific databases

CTDi174924.
WormBaseiY46G5A.31; CE24302; WBGene00001793; gsy-1.

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
InParanoidiQ9U2D9.
KOiK00693.
OMAiIMKRAIY.
OrthoDBiEOG741Z1N.
PhylomeDBiQ9U2D9.

Enzyme and pathway databases

UniPathwayiUPA00164.
ReactomeiREACT_184645. Glycogen synthesis.
REACT_220774. Myoclonic epilepsy of Lafora.

Miscellaneous databases

NextBioi886062.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH GYG-1, PATHWAY, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF PHE-151; GLY-157; TYR-257 AND CYS-261.

Entry informationi

Entry nameiGYS_CAEEL
AccessioniPrimary (citable) accession number: Q9U2D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.