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Protein

Phospholipid-transporting ATPase tat-1

Gene

tat-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Transports phosphatidylserine from the outer to the inner leaflet of the plasma membrane, thereby maintaining the enrichment of this phospholipid in the inner leaflet (PubMed:18436785, PubMed:21170358, PubMed:23427264). Ectopic exposure of phosphatidylserine on the cell surface may result in removal of living cells by neighboring phagocytes (PubMed:18436785). Regulation of the phosphatidylserine distribution in plasma membranes is likely to help in the maintainence and control of the membrane surface charge (PubMed:23427264). Plays a role in the formation of the tubular membrane structure and in membrane trafficking and is specifically involved in the recycling and degradation of endocytic cargo and this is likely with its chaperone protein chat-1 (PubMed:21170358, PubMed:23427264).3 Publications

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei388 – 38814-aspartylphosphate intermediateBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: WormBase

GO - Biological processi

  • endocytosis Source: WormBase
  • lysosome organization Source: WormBase
  • negative regulation of phagocytosis, engulfment Source: WormBase
  • phospholipid translocation Source: WormBase
  • phospholipid transport Source: WormBase
  • positive regulation of phagocytosis, engulfment Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.15. the p-type atpase (p-atpase) superfamily.

Chemistry

SwissLipidsiSLP:000000331.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase tat-1 (EC:3.6.3.1)
Gene namesi
Name:tat-1
ORF Names:Y49E10.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY49E10.11a; CE28133; WBGene00013034; tat-1.
Y49E10.11b; CE36241; WBGene00013034; tat-1.
Y49E10.11c; CE44010; WBGene00013034; tat-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei78 – 9821HelicalSequence analysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Transmembranei318 – 33821HelicalSequence analysisAdd
BLAST
Transmembranei831 – 85121HelicalSequence analysisAdd
BLAST
Transmembranei855 – 87521HelicalSequence analysisAdd
BLAST
Transmembranei901 – 92121HelicalSequence analysisAdd
BLAST
Transmembranei935 – 95521HelicalSequence analysisAdd
BLAST
Transmembranei972 – 99221HelicalSequence analysisAdd
BLAST
Transmembranei1013 – 103321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: WormBase
  • basolateral plasma membrane Source: WormBase
  • cytoplasm Source: WormBase
  • early endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: WormBase
  • integral component of plasma membrane Source: WormBase
  • recycling endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Results in abnormal formation of intestinal vacuoles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11391139Phospholipid-transporting ATPase tat-1PRO_0000424852Add
BLAST

Proteomic databases

EPDiQ9U280.
PaxDbiQ9U280.
PRIDEiQ9U280.

Expressioni

Gene expression databases

ExpressionAtlasiQ9U280. baseline.

Interactioni

Protein-protein interaction databases

BioGridi41845. 1 interaction.
STRINGi6239.Y49E10.11c.2.

Structurei

3D structure databases

ProteinModelPortaliQ9U280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
GeneTreeiENSGT00770000120474.
HOGENOMiHOG000202528.
KOiK14802.
OMAiDLALRCH.
PhylomeDBiQ9U280.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9U280-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTEARDNNR HIHLGKVRDP HHQHAQRFCS NRISTCKYNG FSFLPRFLYE
60 70 80 90 100
QFRRYNNIFF LAIALLQQIP DVSPTGRYTT AVPFLIILSV SALKEIFEDV
110 120 130 140 150
KRRRSDNKVN AFSVEILVDG HWIEKQWKDV SVGDFIRIDN DSLFPADLLL
160 170 180 190 200
LASSEQQGMA YIETSNLDGE TNLKIKQALD ITSTMTSPEK LSQFESEITC
210 220 230 240 250
EPPSRHVNEF NGNIEINGVA RHFGIDQLLL RGARLKNTAW IFGAVIYTGH
260 270 280 290 300
DSKLLMNSKR APLKSGTIDV QTNYRIIFLF FVLVALALIS ATGSEIWRGN
310 320 330 340 350
NIPQAWYLSF LEHDPKGSFL WGVLTFFILY NNLIPISLQV TLEVVRFFQA
360 370 380 390 400
IYINNDIEMY DVNSDSCAIA RTSNLNEELG QVKFIMSDKT GTLTRNVMKF
410 420 430 440 450
KRLSIGSRNY GNNEDDEFAD ASLIEDYRQG DEHSTSILEV LKMMAVCHTV
460 470 480 490 500
VPENKDGQLI YQSSSPDEAA LVRGAASQSV SFHTRQPQKV ICNVFGEDET
510 520 530 540 550
IEILDVIDFT SDRKRMSVIV RDGAGGDIKL YTKGADTVIF ERLEHGKEQE
560 570 580 590 600
EAVEYCTEHL EDYASFGYRT LCFSMRHLTE QEYSQWAPEY KKAILAIDNR
610 620 630 640 650
AKLLADAAEK LERNMILVGA TAIEDKLQEW VPETIQALMA ADIRVWMLTG
660 670 680 690 700
DKRETAINIA HSCALCHTNT ELLIVDKTTY EETYQKLEQF VARAIELEKQ
710 720 730 740 750
EKGFAMVIDG KSLLHALTGE ARKHFGDLAL RCHAVVCCRM SPMQKAEVVE
760 770 780 790 800
MVRKLAKHVV LAIGDGANDV AMIQAANVGV GISGEEGLQA ASASDYAIPR
810 820 830 840 850
FHFLRRLLLV HGAWNHDRSV KVILYSFYKN ICLYIIELWF AMFSAWSGQT
860 870 880 890 900
IFERWTIGMF NVIFTAWPPV VLGLFDHPVP AEQIMKYPAL YASFQNRAFS
910 920 930 940 950
IGNFSLWIGL AIVHSLSLFF LTYATMEHQV VWDNGLTGGW LMLGNCAYTF
960 970 980 990 1000
VVATVCFKAL LECDSWTWPV VVACIGSIGL WIVFVIVYSL VFPHIGGIGA
1010 1020 1030 1040 1050
DMAGMAAIMM SSYTFWLALL FIPLATLLWD LVIKSLFTIA MPTPRELAVM
1060 1070 1080 1090 1100
YNKRTTSFNG FERLASYSSN VLENMRLLTS SLRGSTTGST RSRTASEASL
1110 1120 1130
ALAEQTRYGF AFSQDESSAV AQTELIRNVD STREKPTGR
Length:1,139
Mass (Da):128,411
Last modified:October 1, 2001 - v3
Checksum:i4EB9BABE669824AC
GO
Isoform b (identifier: Q9U280-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1066-1089: SYSSNVLENMRLLTSSLRGSTTGS → RKHVHLHTIIVAIERRLKAVCEWV
     1090-1139: Missing.

Show »
Length:1,089
Mass (Da):123,285
Checksum:iFBAC22424A7B34AE
GO
Isoform c (identifier: Q9U280-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1067-1139: YSSNVLENMR...DSTREKPTGR → SWAAYQGPTK...DEEDGSGTRL

Show »
Length:1,192
Mass (Da):134,455
Checksum:i43C75FDB38B500F2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1066 – 108924SYSSN…STTGS → RKHVHLHTIIVAIERRLKAV CEWV in isoform b. CuratedVSP_053522Add
BLAST
Alternative sequencei1067 – 113973YSSNV…KPTGR → SWAAYQGPTKDGAHVFANRF SLRKRIQPTSTTAASHPSAT SPPPNGYVEKSQLNGKNGKH HRAKSPDYGSTELSTWSTRD EHEVEYKIPRGRKERSSYTN RAFIAEDNNVTSIVVNDEED GSGTRL in isoform c. CuratedVSP_053523Add
BLAST
Alternative sequencei1090 – 113950Missing in isoform b. CuratedVSP_053524Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98866 Genomic DNA. Translation: CAB11550.4.
Z98866 Genomic DNA. Translation: CAE54923.1.
Z98866 Genomic DNA. Translation: CAX51688.2.
PIRiD88601.
T27057.
RefSeqiNP_001022894.1. NM_001027723.2. [Q9U280-1]
NP_001022895.1. NM_001027724.2. [Q9U280-2]
NP_001255165.1. NM_001268236.1. [Q9U280-3]
UniGeneiCel.17289.

Genome annotation databases

EnsemblMetazoaiY49E10.11a.1; Y49E10.11a.1; WBGene00013034. [Q9U280-1]
Y49E10.11a.2; Y49E10.11a.2; WBGene00013034. [Q9U280-1]
GeneIDi176666.
KEGGicel:CELE_Y49E10.11.
UCSCiY49E10.11a. c. elegans. [Q9U280-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z98866 Genomic DNA. Translation: CAB11550.4.
Z98866 Genomic DNA. Translation: CAE54923.1.
Z98866 Genomic DNA. Translation: CAX51688.2.
PIRiD88601.
T27057.
RefSeqiNP_001022894.1. NM_001027723.2. [Q9U280-1]
NP_001022895.1. NM_001027724.2. [Q9U280-2]
NP_001255165.1. NM_001268236.1. [Q9U280-3]
UniGeneiCel.17289.

3D structure databases

ProteinModelPortaliQ9U280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41845. 1 interaction.
STRINGi6239.Y49E10.11c.2.

Chemistry

SwissLipidsiSLP:000000331.

Protein family/group databases

TCDBi3.A.3.8.15. the p-type atpase (p-atpase) superfamily.

Proteomic databases

EPDiQ9U280.
PaxDbiQ9U280.
PRIDEiQ9U280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY49E10.11a.1; Y49E10.11a.1; WBGene00013034. [Q9U280-1]
Y49E10.11a.2; Y49E10.11a.2; WBGene00013034. [Q9U280-1]
GeneIDi176666.
KEGGicel:CELE_Y49E10.11.
UCSCiY49E10.11a. c. elegans. [Q9U280-1]

Organism-specific databases

CTDi176666.
WormBaseiY49E10.11a; CE28133; WBGene00013034; tat-1.
Y49E10.11b; CE36241; WBGene00013034; tat-1.
Y49E10.11c; CE44010; WBGene00013034; tat-1.

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
GeneTreeiENSGT00770000120474.
HOGENOMiHOG000202528.
KOiK14802.
OMAiDLALRCH.
PhylomeDBiQ9U280.

Enzyme and pathway databases

ReactomeiR-CEL-936837. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi893526.
PROiQ9U280.

Gene expression databases

ExpressionAtlasiQ9U280. baseline.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Role of C. elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry."
    Darland-Ransom M., Wang X., Sun C.L., Mapes J., Gengyo-Ando K., Mitani S., Xue D.
    Science 320:528-531(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. "Endocytic sorting and recycling require membrane phosphatidylserine asymmetry maintained by TAT-1/CHAT-1."
    Chen B., Jiang Y., Zeng S., Yan J., Li X., Zhang Y., Zou W., Wang X.
    PLoS Genet. 6:E1001235-E1001235(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Inactivation of Caenorhabditis elegans aminopeptidase DNPP-1 restores endocytic sorting and recycling in tat-1 mutants."
    Li X., Chen B., Yoshina S., Cai T., Yang F., Mitani S., Wang X.
    Mol. Biol. Cell 24:1163-1175(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTAT1_CAEEL
AccessioniPrimary (citable) accession number: Q9U280
Secondary accession number(s): C0P289, Q7JK70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: October 1, 2001
Last modified: May 11, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.