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Protein

Serine/threonine-protein kinase chk-2

Gene

chk-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Phosphorylates and inhibits cdc25 phosphatase, preventing entry into mitosis. Required for nuclear reorganization and homologous chromosome pairing during meiotic prophase.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei199 – 1991ATPPROSITE-ProRule annotation
Active sitei301 – 3011Proton acceptorPROSITE-ProRule annotation
Binding sitei322 – 3221ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi177 – 1848ATPPROSITE-ProRule annotation
Nucleotide bindingi252 – 2587ATPPROSITE-ProRule annotation
Nucleotide bindingi305 – 3062ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of histone H3-K9 methylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-CEL-6804760. Regulation of TP53 Activity through Methylation.
R-CEL-69473. G2/M DNA damage checkpoint.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiQ9U1Y5.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk-2 (EC:2.7.11.1)
Alternative name(s):
Ce-cds-1
Ce-chk-2
Gene namesi
Name:chk-2
Synonyms:cds-1
ORF Names:Y60A3A.12
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY60A3A.12; CE27297; WBGene00000499; chk-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Serine/threonine-protein kinase chk-2PRO_0000085860Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9U1Y5.

Expressioni

Tissue specificityi

Highly expressed in germline tissue.1 Publication

Developmental stagei

Low expression in embryos and L2 larvae, high expression in hermaphrodite adults.1 Publication

Gene expression databases

BgeeiWBGene00000499.

Interactioni

Protein-protein interaction databases

BioGridi45265. 1 interaction.
STRINGi6239.Y60A3A.12.

Structurei

3D structure databases

ProteinModelPortaliQ9U1Y5.
SMRiQ9U1Y5. Positions 45-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 12762FHAPROSITE-ProRule annotationCuratedAdd
BLAST
Domaini170 – 436267Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotationCurated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00800000124190.
HOGENOMiHOG000253056.
InParanoidiQ9U1Y5.
KOiK06641.
OMAiINNEVAC.
OrthoDBiEOG091G08GV.
PhylomeDBiQ9U1Y5.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00498. FHA. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9U1Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRGTKRRRS SAEKPIVVVP VTRDDTMPVD EDLVVGESQC AASKPFAKLV
60 70 80 90 100
GVRRGISSID LADDHFVCGR GSDDAPTNFN FSQVAKDVGL YRFISKIQFS
110 120 130 140 150
IDRDTETRRI YLHDHSRNGT LVNQEMIGKG LSRELMNGDL ISIGIPALII
160 170 180 190 200
FVYESADADH HPEELTKKYH VTSHSLGKGG FGKVLLGYKK SDRSVVAIKQ
210 220 230 240 250
LNTQFSTRCS RAIAKTRDIR NEVEVMKKLS HPNIVAIYDW ITVAKYSYMV
260 270 280 290 300
IEYVGGGEFF SKVVDSKYNR MGLGESLGKY FAFQLIDAIL YLHSVGICHR
310 320 330 340 350
DIKPENILCS DKAERCILKL TDFGMAKNSV NRMKTRCGTP SYNAPEIVAN
360 370 380 390 400
EGVEYTPKVD IWSLGCVLFI TFSGYPPFSE EYTDMTMDEQ VLTGRLIFHA
410 420 430 440 450
QWRRITVETQ NMIKWMLTVE PSNRPSAVEL MSTQWMKCAD CRTAKQDILK
460 470
SIKPISAAAP AALQTTQAGP VKKAKM
Length:476
Mass (Da):53,338
Last modified:October 1, 2001 - v2
Checksum:i9762018F599F9A7B
GO

Sequence cautioni

The sequence BAB15803 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 134SSAE → ARRK in BAB15803 (PubMed:11086161).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041996 mRNA. Translation: BAB20578.1.
AL117207 Genomic DNA. Translation: CAB60407.2.
AB049441 mRNA. Translation: BAB15803.1. Different initiation.
RefSeqiNP_001024271.1. NM_001029100.3.
UniGeneiCel.19391.

Genome annotation databases

EnsemblMetazoaiY60A3A.12; Y60A3A.12; WBGene00000499.
GeneIDi180304.
KEGGicel:CELE_Y60A3A.12.
UCSCiY60A3A.12. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041996 mRNA. Translation: BAB20578.1.
AL117207 Genomic DNA. Translation: CAB60407.2.
AB049441 mRNA. Translation: BAB15803.1. Different initiation.
RefSeqiNP_001024271.1. NM_001029100.3.
UniGeneiCel.19391.

3D structure databases

ProteinModelPortaliQ9U1Y5.
SMRiQ9U1Y5. Positions 45-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45265. 1 interaction.
STRINGi6239.Y60A3A.12.

Proteomic databases

PaxDbiQ9U1Y5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY60A3A.12; Y60A3A.12; WBGene00000499.
GeneIDi180304.
KEGGicel:CELE_Y60A3A.12.
UCSCiY60A3A.12. c. elegans.

Organism-specific databases

CTDi180304.
WormBaseiY60A3A.12; CE27297; WBGene00000499; chk-2.

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00800000124190.
HOGENOMiHOG000253056.
InParanoidiQ9U1Y5.
KOiK06641.
OMAiINNEVAC.
OrthoDBiEOG091G08GV.
PhylomeDBiQ9U1Y5.

Enzyme and pathway databases

ReactomeiR-CEL-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-CEL-6804760. Regulation of TP53 Activity through Methylation.
R-CEL-69473. G2/M DNA damage checkpoint.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiQ9U1Y5.

Miscellaneous databases

PROiQ9U1Y5.

Gene expression databases

BgeeiWBGene00000499.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00498. FHA. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHK2_CAEEL
AccessioniPrimary (citable) accession number: Q9U1Y5
Secondary accession number(s): Q9GR99, Q9GRB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.