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Protein

Serine/threonine-protein kinase chk-2

Gene

chk-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Phosphorylates and inhibits cdc25 phosphatase, preventing entry into mitosis. Required for nuclear reorganization and homologous chromosome pairing during meiotic prophase.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199ATPPROSITE-ProRule annotation1
Active sitei301Proton acceptorPROSITE-ProRule annotation1
Binding sitei322ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 184ATPPROSITE-ProRule annotation8
Nucleotide bindingi252 – 258ATPPROSITE-ProRule annotation7
Nucleotide bindingi305 – 306ATPPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, DNA damage, DNA repair, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-CEL-6804760. Regulation of TP53 Activity through Methylation.
R-CEL-69473. G2/M DNA damage checkpoint.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CEL-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
SignaLinkiQ9U1Y5.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase chk-2 (EC:2.7.11.1)
Alternative name(s):
Ce-cds-1
Ce-chk-2
Gene namesi
Name:chk-2
Synonyms:cds-1
ORF Names:Y60A3A.12
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY60A3A.12; CE27297; WBGene00000499; chk-2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858601 – 476Serine/threonine-protein kinase chk-2Add BLAST476

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9U1Y5.

Expressioni

Tissue specificityi

Highly expressed in germline tissue.1 Publication

Developmental stagei

Low expression in embryos and L2 larvae, high expression in hermaphrodite adults.1 Publication

Gene expression databases

BgeeiWBGene00000499.

Interactioni

Protein-protein interaction databases

BioGridi45265. 1 interactor.
STRINGi6239.Y60A3A.12.

Structurei

3D structure databases

ProteinModelPortaliQ9U1Y5.
SMRiQ9U1Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 127FHAPROSITE-ProRule annotationCuratedAdd BLAST62
Domaini170 – 436Protein kinasePROSITE-ProRule annotationAdd BLAST267

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00800000124190.
HOGENOMiHOG000253056.
InParanoidiQ9U1Y5.
KOiK06641.
OMAiQDIDETH.
OrthoDBiEOG091G08GV.
PhylomeDBiQ9U1Y5.

Family and domain databases

CDDicd00060. FHA. 1 hit.
InterProiView protein in InterPro
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR008984. SMAD_FHA_dom_sf.
PfamiView protein in Pfam
PF00498. FHA. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50006. FHA_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9U1Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRGTKRRRS SAEKPIVVVP VTRDDTMPVD EDLVVGESQC AASKPFAKLV
60 70 80 90 100
GVRRGISSID LADDHFVCGR GSDDAPTNFN FSQVAKDVGL YRFISKIQFS
110 120 130 140 150
IDRDTETRRI YLHDHSRNGT LVNQEMIGKG LSRELMNGDL ISIGIPALII
160 170 180 190 200
FVYESADADH HPEELTKKYH VTSHSLGKGG FGKVLLGYKK SDRSVVAIKQ
210 220 230 240 250
LNTQFSTRCS RAIAKTRDIR NEVEVMKKLS HPNIVAIYDW ITVAKYSYMV
260 270 280 290 300
IEYVGGGEFF SKVVDSKYNR MGLGESLGKY FAFQLIDAIL YLHSVGICHR
310 320 330 340 350
DIKPENILCS DKAERCILKL TDFGMAKNSV NRMKTRCGTP SYNAPEIVAN
360 370 380 390 400
EGVEYTPKVD IWSLGCVLFI TFSGYPPFSE EYTDMTMDEQ VLTGRLIFHA
410 420 430 440 450
QWRRITVETQ NMIKWMLTVE PSNRPSAVEL MSTQWMKCAD CRTAKQDILK
460 470
SIKPISAAAP AALQTTQAGP VKKAKM
Length:476
Mass (Da):53,338
Last modified:October 1, 2001 - v2
Checksum:i9762018F599F9A7B
GO

Sequence cautioni

The sequence BAB15803 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 13SSAE → ARRK in BAB15803 (PubMed:11086161).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041996 mRNA. Translation: BAB20578.1.
AL117207 Genomic DNA. Translation: CAB60407.2.
AB049441 mRNA. Translation: BAB15803.1. Different initiation.
RefSeqiNP_001024271.1. NM_001029100.3.
UniGeneiCel.19391.

Genome annotation databases

EnsemblMetazoaiY60A3A.12; Y60A3A.12; WBGene00000499.
GeneIDi180304.
KEGGicel:CELE_Y60A3A.12.
UCSCiY60A3A.12. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiCHK2_CAEEL
AccessioniPrimary (citable) accession number: Q9U1Y5
Secondary accession number(s): Q9GR99, Q9GRB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: October 1, 2001
Last modified: November 22, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families