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Protein

Integrin-linked protein kinase homolog pat-4

Gene

pat-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable pseudokinase that acts as an adapter protein. Involved in the assembly of dense bodies and M lines during body wall muscle development by recruiting several of their components to integrin-mediated attachment sites (PubMed:12015115). Plays a role in distal tip cell (DTC) migration and in oocyte development probably by regulating the actin cytoskeleton (PubMed:16476426). May be involved in thermotolerance and lifespan (PubMed:24314125).3 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • integrin binding Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • embryo development ending in birth or egg hatching Source: WormBase
  • integrin-mediated signaling pathway Source: WormBase
  • myofibril assembly Source: WormBase
  • protein phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-CEL-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked protein kinase homolog pat-41 Publication
Short name:
ILK homolog1 Publication
Alternative name(s):
Paralyzed and arrested elongation at two-fold protein 41 Publication
Gene namesi
Name:pat-4Imported
ORF Names:C29F9.7Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC29F9.7; CE19706; WBGene00003931; pat-4.

Subcellular locationi

GO - Cellular componenti

  • adherens junction Source: WormBase
  • basal plasma membrane Source: UniProtKB-SubCell
  • integrin complex Source: WormBase
  • M band Source: WormBase
  • striated muscle dense body Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality due to 1.5 fold stage-onset paralysis. Mutant embryos have defects in maintaining polarization of several components involved in the assembly of dense bodies and M lines, such as integrin pat-3, unc-89 and unc-112 to muscle attachment sites (PubMed:12015115). RNAi-mediated knockdown causes sterility resulting from oocyte accumulation in the proximal gonad and distal tip cell (DTC) migration defects (33 percent of animals). Actin cytoskeleton in the proximal gonad appears disorganized (PubMed:16476426). RNAi-mediated knockdown in hatched embryos but not in adults causes adult-onset paralysis characterized by the collapse of myosin filaments in body wall muscles and a decrease in pharyngeal pumping (PubMed:24314125). In L1 larval stage, results in the formation of large myosin aggregates in body wall muscle cells (PubMed:22761445). In adults, causes an increase in lifespan, resistance to heat stress and increased expression of stress response factors hsf-1, sod-3, hsp-16.2 and gst-4 (PubMed:24314125).4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi257 – 2571P → L: Restores the interaction with unc-112 when mutated at "Asp-382" and the localization of mutated unc-112 to integrin adhesion sites in body wall muscles. 1 Publication
Mutagenesisi374 – 3741E → K: No effect on the interaction with unc-112 or pat-6. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Integrin-linked protein kinase homolog pat-4CuratedPRO_0000434505Add
BLAST

Proteomic databases

PaxDbiQ9TZC4.
PRIDEiQ9TZC4.

Expressioni

Tissue specificityi

Expressed in body wall muscle.1 Publication

Developmental stagei

Expression starts in body wall muscle at the 1.5-fold embryonic stage.1 Publication

Gene expression databases

ExpressionAtlasiQ9TZC4. baseline and differential.

Interactioni

Subunit structurei

Interacts (via protein kinase domain) with unc-112 (via N-terminus) (PubMed:12015115, PubMed:24692564). Interacts (via ANK repeats) with unc-97 (via first LIM domain) (PubMed:12015115, PubMed:17662976). Interacts (via protein kinase domain) with pat-6 (via C-terminus CH domain) (PubMed:12781130). May form a complex with unc-112, unc-97 and pat-6 (PubMed:12781130, PubMed:17662976, PubMed:24692564). Does not interact with integrin pat-3 (PubMed:12015115).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pat-6O167854EBI-1564527,EBI-328106
unc-112Q186855EBI-1564527,EBI-1564809

Protein-protein interaction databases

IntActiQ9TZC4. 4 interactions.
STRINGi6239.C29F9.7.

Structurei

3D structure databases

ProteinModelPortaliQ9TZC4.
SMRiQ9TZC4. Positions 20-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati50 – 7930ANK 1Sequence analysisAdd
BLAST
Repeati83 – 11230ANK 2Sequence analysisAdd
BLAST
Repeati116 – 14530ANK 3Sequence analysisAdd
BLAST
Domaini210 – 465256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 3 ANK repeats.UniRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000047828.
InParanoidiQ9TZC4.
KOiK06272.
OMAiRDFNEEH.
OrthoDBiEOG7T1RB0.
PhylomeDBiQ9TZC4.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TZC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSTHYHAH KPNVPIIMED VFGWVREGNA FQVRVWLDDH EHDLNVGDDH
60 70 80 90 100
AFSLLHWAAK GGHVAIAEML LSRGARVNST NMGDDTSLHL AAAHGHRQIV
110 120 130 140 150
VKLLSRKADV NATNEHGMTP LHYACFWGYE QIAEDLISCG AAVNVCNKKG
160 170 180 190 200
MTPLDVCQPM CKNTILEIAQ EHGQSPNDRV PFKDTTWKGT KSRTRDATLS
210 220 230 240 250
RYTGVDVSSL NLITKIAESH SGELWRGKWQ GNDIVARILN VQEVTARISR
260 270 280 290 300
DFQTEFPALR IFAHPNICAV LAAANQPPNL VIISQYMPFG SLYNVLHEQS
310 320 330 340 350
SVVIDHGQAV RFALDIARGM SYLHSLDPML LRFYLSSKHV VVDEELTAKL
360 370 380 390 400
SMADTKFSFQ EVGKAYSPAW MSPEALSRAP EDLNIRAADM WSFAILLWEL
410 420 430 440 450
NTREVPFSDL PPMECGMKIA LEGLRVHIPP GIARNMNRLM NICMNEDPGR
460
RPNFDQIIPI LERMIL
Length:466
Mass (Da):52,260
Last modified:May 1, 2000 - v1
Checksum:i0D13147657A0DF11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CCD66066.1.
AJ249344 mRNA. Translation: CAB77052.1.
PIRiT33574.
RefSeqiNP_497139.1. NM_064738.5.
UniGeneiCel.10243.

Genome annotation databases

EnsemblMetazoaiC29F9.7; C29F9.7; WBGene00003931.
GeneIDi175175.
KEGGicel:CELE_C29F9.7.
UCSCiC29F9.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CCD66066.1.
AJ249344 mRNA. Translation: CAB77052.1.
PIRiT33574.
RefSeqiNP_497139.1. NM_064738.5.
UniGeneiCel.10243.

3D structure databases

ProteinModelPortaliQ9TZC4.
SMRiQ9TZC4. Positions 20-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9TZC4. 4 interactions.
STRINGi6239.C29F9.7.

Proteomic databases

PaxDbiQ9TZC4.
PRIDEiQ9TZC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC29F9.7; C29F9.7; WBGene00003931.
GeneIDi175175.
KEGGicel:CELE_C29F9.7.
UCSCiC29F9.7. c. elegans.

Organism-specific databases

CTDi175175.
WormBaseiC29F9.7; CE19706; WBGene00003931; pat-4.

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000047828.
InParanoidiQ9TZC4.
KOiK06272.
OMAiRDFNEEH.
OrthoDBiEOG7T1RB0.
PhylomeDBiQ9TZC4.

Enzyme and pathway databases

ReactomeiR-CEL-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Miscellaneous databases

NextBioi887074.

Gene expression databases

ExpressionAtlasiQ9TZC4. baseline and differential.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  2. "Integrin-linked kinase regulates phosphorylation of serine 473 of protein kinase B by an indirect mechanism."
    Lynch D.K., Ellis C.A., Edwards P.A., Hiles I.D.
    Oncogene 18:8024-8032(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-466.
  3. "C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes."
    Mackinnon A.C., Qadota H., Norman K.R., Moerman D.G., Williams B.D.
    Curr. Biol. 12:787-797(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UNC-112 AND UNC-97, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-374.
  4. "C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin adhesion complexes in vivo."
    Lin X., Qadota H., Moerman D.G., Williams B.D.
    Curr. Biol. 13:922-932(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAT-6, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-374.
  5. "pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans."
    Xu X., Rongali S.C., Miles J.P., Lee K.D., Lee M.
    Exp. Cell Res. 312:1475-1483(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "UNC-97/PINCH is involved in the assembly of integrin cell adhesion complexes in Caenorhabditis elegans body wall muscle."
    Norman K.R., Cordes S., Qadota H., Rahmani P., Moerman D.G.
    Dev. Biol. 309:45-55(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UNC-97, SUBCELLULAR LOCATION.
  7. "A molecular mechanism for the requirement of PAT-4 (integrin-linked kinase (ILK)) for the localization of UNC-112 (Kindlin) to integrin adhesion sites."
    Qadota H., Moerman D.G., Benian G.M.
    J. Biol. Chem. 287:28537-28551(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. "Integrin-linked kinase modulates longevity and thermotolerance in C. elegans through neuronal control of HSF-1."
    Kumsta C., Ching T.T., Nishimura M., Davis A.E., Gelino S., Catan H.H., Yu X., Chu C.C., Ong B., Panowski S.H., Baird N., Bodmer R., Hsu A.L., Hansen M.
    Aging Cell 13:419-430(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Suppressor mutations suggest a surface on PAT-4 (Integrin-linked Kinase) that interacts with UNC-112 (Kindlin)."
    Qadota H., Luo Y., Matsunaga Y., Park A.S., Gernert K.M., Benian G.M.
    J. Biol. Chem. 289:14252-14262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UNC-112, MUTAGENESIS OF PRO-257.

Entry informationi

Entry nameiILKH_CAEEL
AccessioniPrimary (citable) accession number: Q9TZC4
Secondary accession number(s): Q9NG00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: December 9, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.