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Protein

Integrin-linked protein kinase homolog pat-4

Gene

pat-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable pseudokinase that acts as an adapter protein. Involved in the assembly of dense bodies and M lines during body wall muscle development by recruiting several of their components including integrin pat-3, cpna-1, unc-89 and unc-112 to integrin-mediated attachment sites (PubMed:12015115, PubMed:23283987). Plays a role in distal tip cell (DTC) migration and in oocyte development probably by regulating the actin cytoskeleton (PubMed:16476426). During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles (PubMed:16495308). May be involved in thermotolerance and lifespan (PubMed:24314125).5 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • integrin binding Source: UniProtKB
  • protein kinase activity Source: GO_Central
  • protein serine/threonine kinase activity Source: WormBase
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • cell-matrix adhesion Source: GO_Central
  • embryo development ending in birth or egg hatching Source: WormBase
  • hermaphrodite genitalia morphogenesis Source: UniProtKB
  • integrin-mediated signaling pathway Source: WormBase
  • MAPK cascade Source: GO_Central
  • myofibril assembly Source: WormBase
  • positive regulation of distal tip cell migration Source: UniProtKB
  • positive regulation of establishment of protein localization Source: UniProtKB
  • positive regulation of myosin II filament organization Source: UniProtKB
  • positive regulation of ovulation Source: UniProtKB
  • positive regulation of sarcomere organization Source: UniProtKB
  • positive regulation of smooth muscle contraction Source: UniProtKB
  • protein phosphorylation Source: WormBase
  • regulation of actin cytoskeleton organization Source: UniProtKB
  • striated muscle cell development Source: UniProtKB
  • substrate adhesion-dependent cell spreading Source: GO_Central

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked protein kinase homolog pat-41 Publication
Short name:
ILK homolog1 Publication
Alternative name(s):
Paralyzed and arrested elongation at two-fold protein 41 Publication
Gene namesi
Name:pat-4Imported
ORF Names:C29F9.7Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC29F9.7; CE19706; WBGene00003931; pat-4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality due to 1.5 fold stage-onset paralysis. Mutant embryos have defects in maintaining polarization of several components involved in the assembly of dense bodies and M lines, such as integrin pat-3, unc-89 and unc-112 to muscle attachment sites (PubMed:12015115). RNAi-mediated knockdown causes sterility resulting from oocyte accumulation in the proximal gonad and distal tip cell (DTC) migration defects (33 percent of animals). Actin cytoskeleton in the proximal gonad appears disorganized (PubMed:16476426). RNAi-mediated knockdown in hatched embryos but not in adults causes adult-onset paralysis characterized by the collapse of myosin filaments in body wall muscles and a decrease in pharyngeal pumping (PubMed:24314125). In L1 larval stage, results in the formation of large myosin aggregates in body wall muscle cells (PubMed:22761445). In L4 larval stage, causes ectopic membrane extensions from body wall muscles (PubMed:16495308). In adults, causes an increase in lifespan, resistance to heat stress and increased expression of stress response factors hsf-1, sod-3, hsp-16.2 and gst-4 (PubMed:24314125).5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257P → L: Restores the interaction with unc-112 when mutated at "Asp-382" and the localization of mutated unc-112 to integrin adhesion sites in body wall muscles. 1 Publication1
Mutagenesisi374E → K: No effect on the interaction with unc-112 or pat-6. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004345051 – 466Integrin-linked protein kinase homolog pat-4CuratedAdd BLAST466

Proteomic databases

EPDiQ9TZC4.
PaxDbiQ9TZC4.
PeptideAtlasiQ9TZC4.
PRIDEiQ9TZC4.

Expressioni

Tissue specificityi

Expressed in body wall muscle.2 Publications

Developmental stagei

Expression starts in body wall muscles at the 1.5-fold embryonic stage.2 Publications

Gene expression databases

BgeeiWBGene00003931.

Interactioni

Subunit structurei

Interacts (via protein kinase domain) with unc-112 (via N-terminus) (PubMed:12015115, PubMed:24692564). Interacts (via ANK repeats) with unc-97 (via first LIM domain) (PubMed:12015115, PubMed:17662976). Interacts (via protein kinase domain) with pat-6 (via C-terminus CH domain) (PubMed:12781130). May form a complex with unc-112, unc-97 and pat-6 (PubMed:12781130, PubMed:17662976, PubMed:24692564). Does not interact with integrin pat-3 (PubMed:12015115).4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • integrin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9TZC4. 4 interactors.
STRINGi6239.C29F9.7.

Structurei

3D structure databases

ProteinModelPortaliQ9TZC4.
SMRiQ9TZC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 79ANK 1Sequence analysisAdd BLAST30
Repeati83 – 112ANK 2Sequence analysisAdd BLAST30
Repeati116 – 145ANK 3Sequence analysisAdd BLAST30
Domaini210 – 465Protein kinasePROSITE-ProRule annotationAdd BLAST256

Domaini

The protein kinase domain is predicted to be catalytically inactive.PROSITE-ProRule annotation

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00900000140790.
HOGENOMiHOG000047828.
InParanoidiQ9TZC4.
KOiK06272.
OMAiFSFQEVG.
OrthoDBiEOG091G05IK.
PhylomeDBiQ9TZC4.

Family and domain databases

CDDicd00204. ANK. 1 hit.
cd14057. PK_ILK. 1 hit.
Gene3Di1.25.40.20. 2 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR011009. Kinase-like_dom_sf.
IPR035692. PK_ILK.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
PfamiView protein in Pfam
PF12796. Ank_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 3 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9TZC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSTHYHAH KPNVPIIMED VFGWVREGNA FQVRVWLDDH EHDLNVGDDH
60 70 80 90 100
AFSLLHWAAK GGHVAIAEML LSRGARVNST NMGDDTSLHL AAAHGHRQIV
110 120 130 140 150
VKLLSRKADV NATNEHGMTP LHYACFWGYE QIAEDLISCG AAVNVCNKKG
160 170 180 190 200
MTPLDVCQPM CKNTILEIAQ EHGQSPNDRV PFKDTTWKGT KSRTRDATLS
210 220 230 240 250
RYTGVDVSSL NLITKIAESH SGELWRGKWQ GNDIVARILN VQEVTARISR
260 270 280 290 300
DFQTEFPALR IFAHPNICAV LAAANQPPNL VIISQYMPFG SLYNVLHEQS
310 320 330 340 350
SVVIDHGQAV RFALDIARGM SYLHSLDPML LRFYLSSKHV VVDEELTAKL
360 370 380 390 400
SMADTKFSFQ EVGKAYSPAW MSPEALSRAP EDLNIRAADM WSFAILLWEL
410 420 430 440 450
NTREVPFSDL PPMECGMKIA LEGLRVHIPP GIARNMNRLM NICMNEDPGR
460
RPNFDQIIPI LERMIL
Length:466
Mass (Da):52,260
Last modified:May 1, 2000 - v1
Checksum:i0D13147657A0DF11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA. Translation: CCD66066.1.
AJ249344 mRNA. Translation: CAB77052.1.
PIRiT33574.
RefSeqiNP_497139.1. NM_064738.5.
UniGeneiCel.10243.

Genome annotation databases

EnsemblMetazoaiC29F9.7; C29F9.7; WBGene00003931.
GeneIDi175175.
KEGGicel:CELE_C29F9.7.
UCSCiC29F9.7. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiILKH_CAEEL
AccessioniPrimary (citable) accession number: Q9TZC4
Secondary accession number(s): Q9NG00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families