Q9TXQ1 (PME5_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(ADP-ribose) polymerase pme-5
EC=2.4.2.30

Alternative name(s):
Poly ADP-ribose metabolism enzyme 5

Gene names

Name:	pme-5
ORF Names:	ZK1005.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	2276 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Poly[ADP-ribose] polymerases modify various nuclear proteins by poly(ADP-ribosyl)ation, a post-translational modification synthesized after DNA damage that appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks and programmed cell death. Ref.1
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Probable.
Developmental stage	Expressed at low level in embryos compared to larvae. Peaks in young adults. Ref.1
Induction	Following irradiation; this induction is dependent of hus-1. Ref.1
Sequence similarities	Contains 16 ANK repeats. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat
Ligand	NAD
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of programmed cell death Inferred from mutant phenotype. Source: WormBase protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro response to gamma radiation Inferred from expression pattern. Source: WormBase
Cellular component	nucleus Inferred from direct assay. Source: WormBase
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q9TXQ1-1)

Also known as: PME-5L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q9TXQ1-2)

Also known as: PME-5S;

The sequence of this isoform differs from the canonical sequence as follows:
     852-889: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2276	2276	Poly(ADP-ribose) polymerase pme-5		PRO_0000211337
Regions
	Repeat	345 – 374	30	ANK 1
	Repeat	378 – 407	30	ANK 2
	Repeat	411 – 440	30	ANK 3
	Repeat	461 – 490	30	ANK 4
	Repeat	498 – 527	30	ANK 5
	Repeat	531 – 560	30	ANK 6
	Repeat	564 – 593	30	ANK 7
	Repeat	598 – 627	30	ANK 8
	Repeat	675 – 725	51	ANK 9
	Repeat	729 – 758	30	ANK 10
	Repeat	970 – 999	30	ANK 11
	Repeat	1171 – 1200	30	ANK 12
	Repeat	1204 – 1233	30	ANK 13
	Repeat	1472 – 1501	30	ANK 14
	Repeat	1505 – 1535	31	ANK 15
	Repeat	1662 – 1706	45	ANK 16
	Domain	1910 – 2045	136	PARP alpha-helical
	Domain	2047 – 2276	230	PARP catalytic
	Compositional bias	55 – 100	46	Lys-rich
	Compositional bias	1613 – 1621	9	Poly-Asp
Natural variations
	Alternative sequence	852 – 889	38	Missing in isoform b.		VSP_012461

Sequences

	Sequence		Length	Mass (Da)
	Isoform a (PME-5L) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8D1ADA31DBD2794F	FASTA	2,276	254,576
	10 20 30 40 50 60 MARRVNKKKS PVKAARKIDG QIGRRVVDGV RAKSSRQRHQ AVLYQAPTPT VIRRKTTKTA 70 80 90 100 110 120 IVKKTVVVVK KGGKVVKKSS KTGQKVKAVK APKVKAPSKK GNDRLTPRVI TEYQENPFFY 130 140 150 160 170 180 DPQVPEYISA SVYHRWITRA VRNGNMKEIK DYYKSKKCQK SAIYTSFAYS FDTSACDEAL 190 200 210 220 230 240 RQDIKFATEF FKMNNKMEVD NSYHPGKEPN LLQKKTTGRK NYYMLGRHTR QIEMGRGGKE 250 260 270 280 290 300 GNNALLNYDT RTDEPNPLTK LIEDNVTYTK LYQLCKIPDG PIVEHHIEMH FVTAVRMGHR 310 320 330 340 350 360 DLASALAQGP VKMHCNDLHR ATLKDQKLPA KILPVSVAKK AYMNKNITPL HTAAISNSTH 370 380 390 400 410 420 MLEAMRAVYP TINIPDQDNW YTMHYAACAP GTAPMEFLLK NGGSVTMLTK QTETPLHVAA 430 440 450 460 470 480 RAGRAVNCTF LMKEMLDLEK GDDGESTIRA DRSIINARTR SGNSALHLAV LRNNLDVVDA 490 500 510 520 530 540 LLAEPTIVVD NPTSTGQNRL TPLMMACGKG YLEMAKKLVE KGALVEGKDK KKRTPLIHAM 550 560 570 580 590 600 LNGQIHTAAF LLAKGASLTL ADSSGNTAAH YAAAYGFLDC LKLLASIDDN ILSEPNDWQL 610 620 630 640 650 660 YPLSVAYLKG HYGIVTWLLE GPHKDKANIN AKDNNGATLL SNLLSYADET MHKELLSQIE 670 680 690 700 710 720 YLVARKADAS LADSSGQTPL HLFSMQRIIL KGSGEAAEND AMRMTLDNYK KCFNTLIKAG 730 740 750 760 770 780 AKVDVYDHED NTPLHYALTN GNLMLFNLML DKVANKRNLF EKWANHQNFL HEILALPMKV 790 800 810 820 830 840 YGDQVLWKGE TLTKPAYDVL PILKELHENL PDLFEKWISE VNKAGYSPIV EAIKQYQALA 850 860 870 880 890 900 ANKKLRGEAD QNGNPGFGNA IARGRVHNQF GRDRMNQSQA PHCDSYELKT FISTVNELFE 910 920 930 940 950 960 WVIRLGPFQL TQKYINSENS AAVTLANLAM SIPIECGRHQ QNQLALFKIL IKLSKEFNKV 970 980 990 1000 1010 1020 DEFLTQKNEK DDVLIVQAIM FDKPNVVELI LDTASEMHLI HGTHNAIKEN ELEVVVHKTI 1030 1040 1050 1060 1070 1080 IMYMIEMRMW ELIPKVNASS EFWKSKDAKG NSVWHYAARV NSHKTVGLFK MIESKGVRRE 1090 1100 1110 1120 1130 1140 TNDDGRSVLH VATLACDGSA DSVLEPIAWL STRCPIDAVD KFNRTALHYA FGNENDFKEG 1150 1160 1170 1180 1190 1200 NVPFGESDPI AVVSLLSSLI RPEQIEIADV NGNTILHLAA IKNSTICLMT LIRKKCHVDL 1210 1220 1230 1240 1250 1260 KNKDGNTPLA LAVHHGRQSS ALTLIQANAD VTEKIFVPAL KPTSDFDQNS SGTEAEKFWK 1270 1280 1290 1300 1310 1320 WHGKEKKVLE DLHTTIPASV VSKGGSWEAM VYVLLDVLGQ NTGSMAQLTD AALRRGQLNL 1330 1340 1350 1360 1370 1380 ANQLLKSIEA LIDGAVLNSS YDLLDTFAEK CFGALTSEET IEKTVLNRII LTRGLGLKQP 1390 1400 1410 1420 1430 1440 ETMKIIRTAL QNGNWNLLNF LKSEMGTAWK NQKIETPTEN PIRSLLIYMN EKSVSSEAIG 1450 1460 1470 1480 1490 1500 FLEELRQMRG VNIDALCQLE IPGKFKKILD YGLIPPISFA VLQENPNMIR ALRNAGASLK 1510 1520 1530 1540 1550 1560 TQDDYGRTPL MYAIMTNNRS VVDAIVGDGK LAVVLHKQKA VATGPRCVAV PMRFGATSRA 1570 1580 1590 1600 1610 1620 FIPAAAFASV PARVESDEEE EDNSGSESGE DGAASENKSE HGSENGESGN GSDDEDDDDD 1630 1640 1650 1660 1670 1680 DSSPPPAKKS RIAKEAAGPS TGPKRKKLVI TDPSLFSARD HKENNPLHYF IEPLAWENVE 1690 1700 1710 1720 1730 1740 LLGDLAAANK TAIVQCLIDK RSPNPIELAA MKMNRRMKSE MLKIVKNAAF PRPIKETKLT 1750 1760 1770 1780 1790 1800 LQQVHIEPLS DVDEDAAKFL AKWVEEKDKK KTSEAPKPHK SSTYSTNGLV SFCDETQQYF 1810 1820 1830 1840 1850 1860 DVLMNKTDLM YGRCGFHNFY RMQIIKRRDA ELFILFTNWG RIGSGMGEFQ TTPFNSLELA 1870 1880 1890 1900 1910 1920 AKEFKSIFKS KSGNEWAPLA NFRDMPKKYR LVETDSTPTS LAEIELTWKK NTEKDPIRRM 1930 1940 1950 1960 1970 1980 IADISDAKTL KTYASQVQMY GGSSQPFGRF TKENIEKAKL VLDKLEKNAN RIKQMVEAQT 1990 2000 2010 2020 2030 2040 GVVESNLLDA YITTSELSGD YYSLIPSGEY EFSNLTRLDN VEEIARHRAR LNRCQEIETA 2050 2060 2070 2080 2090 2100 TRLLCAAEFR QDLDRVDYIR SAIQCEYRLE TPDSDISQRL LQWIHNSGGK QAKVKMILEI 2110 2120 2130 2140 2150 2160 SPMLSTEKFE PFVNDDNQKF LWHGTKATNL MSILKNGFLI DPPSACKNGN LFGSGIYLAD 2170 2180 2190 2200 2210 2220 SFEKSTHYCQ PSAGGINYML VCQTALGKVR TLDTIPYHYM NQSSSSAEKY EDTLHYIGDR 2230 2240 2250 2260 2270 FPAGSLTNDG VGMPLLPLRK RDPIQGSNYG FGTLDFSEYI VRNPNRVLPK YIVMYK
	Isoform b (PME-5S) [UniParc]. Checksum: 324E3802BD22C20C	FASTA	2,238	250,351
	10 20 30 40 50 60 MARRVNKKKS PVKAARKIDG QIGRRVVDGV RAKSSRQRHQ AVLYQAPTPT VIRRKTTKTA 70 80 90 100 110 120 IVKKTVVVVK KGGKVVKKSS KTGQKVKAVK APKVKAPSKK GNDRLTPRVI TEYQENPFFY 130 140 150 160 170 180 DPQVPEYISA SVYHRWITRA VRNGNMKEIK DYYKSKKCQK SAIYTSFAYS FDTSACDEAL 190 200 210 220 230 240 RQDIKFATEF FKMNNKMEVD NSYHPGKEPN LLQKKTTGRK NYYMLGRHTR QIEMGRGGKE 250 260 270 280 290 300 GNNALLNYDT RTDEPNPLTK LIEDNVTYTK LYQLCKIPDG PIVEHHIEMH FVTAVRMGHR 310 320 330 340 350 360 DLASALAQGP VKMHCNDLHR ATLKDQKLPA KILPVSVAKK AYMNKNITPL HTAAISNSTH 370 380 390 400 410 420 MLEAMRAVYP TINIPDQDNW YTMHYAACAP GTAPMEFLLK NGGSVTMLTK QTETPLHVAA 430 440 450 460 470 480 RAGRAVNCTF LMKEMLDLEK GDDGESTIRA DRSIINARTR SGNSALHLAV LRNNLDVVDA 490 500 510 520 530 540 LLAEPTIVVD NPTSTGQNRL TPLMMACGKG YLEMAKKLVE KGALVEGKDK KKRTPLIHAM 550 560 570 580 590 600 LNGQIHTAAF LLAKGASLTL ADSSGNTAAH YAAAYGFLDC LKLLASIDDN ILSEPNDWQL 610 620 630 640 650 660 YPLSVAYLKG HYGIVTWLLE GPHKDKANIN AKDNNGATLL SNLLSYADET MHKELLSQIE 670 680 690 700 710 720 YLVARKADAS LADSSGQTPL HLFSMQRIIL KGSGEAAEND AMRMTLDNYK KCFNTLIKAG 730 740 750 760 770 780 AKVDVYDHED NTPLHYALTN GNLMLFNLML DKVANKRNLF EKWANHQNFL HEILALPMKV 790 800 810 820 830 840 YGDQVLWKGE TLTKPAYDVL PILKELHENL PDLFEKWISE VNKAGYSPIV EAIKQYQALA 850 860 870 880 890 900 ANKKLRGEAD QTFISTVNEL FEWVIRLGPF QLTQKYINSE NSAAVTLANL AMSIPIECGR 910 920 930 940 950 960 HQQNQLALFK ILIKLSKEFN KVDEFLTQKN EKDDVLIVQA IMFDKPNVVE LILDTASEMH 970 980 990 1000 1010 1020 LIHGTHNAIK ENELEVVVHK TIIMYMIEMR MWELIPKVNA SSEFWKSKDA KGNSVWHYAA 1030 1040 1050 1060 1070 1080 RVNSHKTVGL FKMIESKGVR RETNDDGRSV LHVATLACDG SADSVLEPIA WLSTRCPIDA 1090 1100 1110 1120 1130 1140 VDKFNRTALH YAFGNENDFK EGNVPFGESD PIAVVSLLSS LIRPEQIEIA DVNGNTILHL 1150 1160 1170 1180 1190 1200 AAIKNSTICL MTLIRKKCHV DLKNKDGNTP LALAVHHGRQ SSALTLIQAN ADVTEKIFVP 1210 1220 1230 1240 1250 1260 ALKPTSDFDQ NSSGTEAEKF WKWHGKEKKV LEDLHTTIPA SVVSKGGSWE AMVYVLLDVL 1270 1280 1290 1300 1310 1320 GQNTGSMAQL TDAALRRGQL NLANQLLKSI EALIDGAVLN SSYDLLDTFA EKCFGALTSE 1330 1340 1350 1360 1370 1380 ETIEKTVLNR IILTRGLGLK QPETMKIIRT ALQNGNWNLL NFLKSEMGTA WKNQKIETPT 1390 1400 1410 1420 1430 1440 ENPIRSLLIY MNEKSVSSEA IGFLEELRQM RGVNIDALCQ LEIPGKFKKI LDYGLIPPIS 1450 1460 1470 1480 1490 1500 FAVLQENPNM IRALRNAGAS LKTQDDYGRT PLMYAIMTNN RSVVDAIVGD GKLAVVLHKQ 1510 1520 1530 1540 1550 1560 KAVATGPRCV AVPMRFGATS RAFIPAAAFA SVPARVESDE EEEDNSGSES GEDGAASENK 1570 1580 1590 1600 1610 1620 SEHGSENGES GNGSDDEDDD DDDSSPPPAK KSRIAKEAAG PSTGPKRKKL VITDPSLFSA 1630 1640 1650 1660 1670 1680 RDHKENNPLH YFIEPLAWEN VELLGDLAAA NKTAIVQCLI DKRSPNPIEL AAMKMNRRMK 1690 1700 1710 1720 1730 1740 SEMLKIVKNA AFPRPIKETK LTLQQVHIEP LSDVDEDAAK FLAKWVEEKD KKKTSEAPKP 1750 1760 1770 1780 1790 1800 HKSSTYSTNG LVSFCDETQQ YFDVLMNKTD LMYGRCGFHN FYRMQIIKRR DAELFILFTN 1810 1820 1830 1840 1850 1860 WGRIGSGMGE FQTTPFNSLE LAAKEFKSIF KSKSGNEWAP LANFRDMPKK YRLVETDSTP 1870 1880 1890 1900 1910 1920 TSLAEIELTW KKNTEKDPIR RMIADISDAK TLKTYASQVQ MYGGSSQPFG RFTKENIEKA 1930 1940 1950 1960 1970 1980 KLVLDKLEKN ANRIKQMVEA QTGVVESNLL DAYITTSELS GDYYSLIPSG EYEFSNLTRL 1990 2000 2010 2020 2030 2040 DNVEEIARHR ARLNRCQEIE TATRLLCAAE FRQDLDRVDY IRSAIQCEYR LETPDSDISQ 2050 2060 2070 2080 2090 2100 RLLQWIHNSG GKQAKVKMIL EISPMLSTEK FEPFVNDDNQ KFLWHGTKAT NLMSILKNGF 2110 2120 2130 2140 2150 2160 LIDPPSACKN GNLFGSGIYL ADSFEKSTHY CQPSAGGINY MLVCQTALGK VRTLDTIPYH 2170 2180 2190 2200 2210 2220 YMNQSSSSAE KYEDTLHYIG DRFPAGSLTN DGVGMPLLPL RKRDPIQGSN YGFGTLDFSE 2230 YIVRNPNRVL PKYIVMYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The C. elegans gene pme-5: molecular cloning and role in the DNA-damage response of a tankyrase orthologue." Gravel C., Stergiou L., Gagnon S.N., Desnoyers S. DNA Repair 3:171-182(2004) [PubMed: 14706351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, DEVELOPMENTAL STAGE, INDUCTION. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF548287 mRNA. Translation: AAN40683.1. FO081160 Genomic DNA. Translation: CCD69563.1. FO081160 Genomic DNA. Translation: CCD69564.1.
PIR	C88948. D88948.
RefSeq	NP_001024306.1. NM_001029135.2. NP_503401.3. NM_071000.5.
UniGene	Cel.17725.
3D structure databases
ProteinModelPortal	Q9TXQ1.
SMR	Q9TXQ1. Positions 286-775, 1017-1235, 1473-1529, 1776-1895, 1910-2276.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9TXQ1.
Proteomic databases
PRIDE	Q9TXQ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK1005.1a; ZK1005.1a; ZK1005.1.
GeneID	178623.
KEGG	cel:ZK1005.1.
UCSC	ZK1005.1b. c. elegans.
Organism-specific databases
CTD	178623.
WormBase	ZK1005.1a; CE24712; WBGene00004053; pme-5. ZK1005.1b; CE33259; WBGene00004053; pme-5.
Phylogenomic databases
eggNOG	meNOG05666.
GeneTree	EMGT00050000000757.
InParanoid	Q9TXQ1.
OMA	KANINAK.
PhylomeDB	Q9TXQ1.
Gene expression databases
ArrayExpress	Q9TXQ1.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004102. Poly(ADP-ribose)pol_reg_dom. IPR008893. WGR_domain. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 9 hits. G3DSA:2.20.140.10. G3DSA:2.20.140.10. 1 hit. G3DSA:1.20.142.10. PARP_reg. 1 hit.
Pfam	PF12796. Ank_2. 4 hits. PF00644. PARP. 1 hit. PF02877. PARP_reg. 1 hit. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00248. ANK. 16 hits. SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 3 hits. SSF47587. PARP_reg. 1 hit. SSF142921. SSF142921. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 3 hits. PS50088. ANK_REPEAT. 6 hits. PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	901888.

Entry information

Entry name

PME5_CAEEL

Accession

Primary (citable) accession number: Q9TXQ1
Secondary accession number(s): Q8I0N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project