Reviewed, UniProtKB/Swiss-Prot Q9TWL9 (COMA_CONMA)

Names and origin

Protein names	Recommended name:     Conodipine-M alpha chain     EC=3.1.1.4
Organism	Conus magus (Magus cone) (Magician's cone snail)
Taxonomic identifier	6492 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Mollusca › Gastropoda › Orthogastropoda › Apogastropoda › Caenogastropoda › Sorbeoconcha › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	77 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conodipine-M catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This activity may be supported by the alpha chain. Conodipine-M inhibits the binding of isradipine (a ligand specific for L-type calcium channel) to L-type calcium channels. It is inhibited by linoleoyl amide and MG14.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Calcium.
Subunit structure	Conodipine-M consists of 2 subunits alpha and beta, which may be linked to each other through one or more disulfide bonds.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Sequence similarities	Belongs to the phospholipase A2 family. Highly divergent.
Mass spectrometry	Molecular mass is 8571 Da from positions 1 - 77. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium
Molecular function	Hydrolase Toxin
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 77	77	Conodipine-M alpha chain		PRO_0000086866
Sites
	Active site	36	1	Potential
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid

Sequences

	Sequence		Length	Mass (Da)
	Q9TWL9-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 73861D7587479D8C	FASTA	77	8,492
	10 20 30 40 50 60 QXPSTAELCK INSNACSVPF SXIPCQKXFL AACDRHDTCY HCGKHFGFKQ DDCDDAFFRD 70 MTALCAHGTD DEGXCPX

References

[1]

"Conodipine-M, a novel phospholipase A2 isolated from the venom of the marine snail Conus magus." McIntosh J.M., Ghomashchi F., Gelb M.H., Dooley D.J., Stoehr S.J., Giordani A.B., Naisbitt S.R., Olivera B.M. J. Biol. Chem. 270:3518-3526(1995) [PubMed: 7876086] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.4. 292750.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

COMA_CONMA

Accession

Primary (citable) accession number: Q9TWL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	June 16, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)