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Q9TVW2

- ADK_TOXGO

UniProt

Q9TVW2 - ADK_TOXGO

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Protein

Adenosine kinase

Gene

AK

Organism
Toxoplasma gondii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

ATP-dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. It is a key purine metabolic enzyme in the opportunistic parasitic protozoan toxoplasma gondii as it cannot synthesize purines de novo.

Catalytic activityi

ATP + adenosine = ADP + AMP.

Cofactori

Mg2+Note: Binds 1 Mg(2+) ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi185 – 1851Magnesium; via carbonyl oxygen
Metal bindingi188 – 1881Magnesium; via carbonyl oxygen
Metal bindingi191 – 1911Magnesium; via carbonyl oxygen
Active sitei318 – 3181

GO - Molecular functioni

  1. adenosine kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. phosphotransferase activity, alcohol group as acceptor Source: InterPro

GO - Biological processi

  1. AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00588; UER00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine kinase (EC:2.7.1.20)
Short name:
AK
Alternative name(s):
Adenosine 5'-phosphotransferase
Gene namesi
Name:AK
OrganismiToxoplasma gondii
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Adenosine kinasePRO_0000080056Add
BLAST

Interactioni

Structurei

Secondary structure

1
363
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 184Combined sources
Beta strandi22 – 287Combined sources
Helixi31 – 366Combined sources
Beta strandi44 – 463Combined sources
Helixi49 – 568Combined sources
Helixi58 – 614Combined sources
Beta strandi64 – 696Combined sources
Helixi70 – 8213Combined sources
Beta strandi87 – 948Combined sources
Helixi98 – 11013Combined sources
Beta strandi113 – 1186Combined sources
Beta strandi125 – 1328Combined sources
Beta strandi135 – 1417Combined sources
Helixi143 – 1475Combined sources
Helixi154 – 1574Combined sources
Turni158 – 1603Combined sources
Beta strandi163 – 1675Combined sources
Helixi168 – 1714Combined sources
Helixi176 – 18611Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi192 – 1965Combined sources
Helixi200 – 2056Combined sources
Helixi207 – 2159Combined sources
Beta strandi218 – 2236Combined sources
Helixi224 – 23411Combined sources
Helixi239 – 2413Combined sources
Helixi249 – 26416Combined sources
Beta strandi274 – 2785Combined sources
Beta strandi284 – 2896Combined sources
Turni291 – 2933Combined sources
Beta strandi295 – 3006Combined sources
Helixi307 – 3093Combined sources
Helixi316 – 32914Combined sources
Helixi334 – 34916Combined sources
Beta strandi351 – 3533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DGMX-ray1.80A1-363[»]
1LIIX-ray1.73A1-363[»]
1LIJX-ray1.86A1-363[»]
1LIKX-ray2.55A1-363[»]
1LIOX-ray2.50A1-363[»]
2A9YX-ray1.35A1-363[»]
2A9ZX-ray1.35A1-363[»]
2AA0X-ray1.75A1-363[»]
2AB8X-ray1.75A1-363[»]
2ABSX-ray1.10A1-363[»]
ProteinModelPortaliQ9TVW2.
SMRiQ9TVW2. Positions 10-359.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9TVW2.

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9TVW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVDSSNSAT GPMRVFAIGN PILDLVAEVP SSFLDEFFLK RGDATLATPE
60 70 80 90 100
QMRIYSTLDQ FNPTSLPGGS ALNSVRVVQK LLRKPGSAGY MGAIGDDPRG
110 120 130 140 150
QVLKELCDKE GLATRFMVAP GQSTGVCAVL INEKERTLCT HLGACGSFRL
160 170 180 190 200
PEDWTTFASG ALIFYATAYT LTATPKNALE VAGYAHGIPN AIFTLNLSAP
210 220 230 240 250
FCVELYKDAM QSLLLHTNIL FGNEEEFAHL AKVHNLVAAE KTALSTANKE
260 270 280 290 300
HAVEVCTGAL RLLTAGQNTG ATKLVVMTRG HNPVIAAEQT ADGTVVVHEV
310 320 330 340 350
GVPVVAAEKI VDTNGAGDAF VGGFLYALSQ GKTVKQCIMC GNACAQDVIQ
360
HVGFSLSFTS LPC
Length:363
Mass (Da):38,356
Last modified:May 1, 2000 - v1
Checksum:i6C621C7D5A56739C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128274 Genomic DNA. Translation: AAF01261.1.
AF128275 mRNA. Translation: AAF01262.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128274 Genomic DNA. Translation: AAF01261.1 .
AF128275 mRNA. Translation: AAF01262.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DGM X-ray 1.80 A 1-363 [» ]
1LII X-ray 1.73 A 1-363 [» ]
1LIJ X-ray 1.86 A 1-363 [» ]
1LIK X-ray 2.55 A 1-363 [» ]
1LIO X-ray 2.50 A 1-363 [» ]
2A9Y X-ray 1.35 A 1-363 [» ]
2A9Z X-ray 1.35 A 1-363 [» ]
2AA0 X-ray 1.75 A 1-363 [» ]
2AB8 X-ray 1.75 A 1-363 [» ]
2ABS X-ray 1.10 A 1-363 [» ]
ProteinModelPortali Q9TVW2.
SMRi Q9TVW2. Positions 10-359.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q9TVW2.
ChEMBLi CHEMBL2982.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00588 ; UER00659 .

Miscellaneous databases

EvolutionaryTracei Q9TVW2.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
InterProi IPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view ]
PANTHERi PTHR10584:SF24. PTHR10584:SF24. 1 hit.
Pfami PF00294. PfkB. 1 hit.
[Graphical view ]
PRINTSi PR00989. ADENOKINASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
PROSITEi PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Insertional tagging of at least two loci associated with resistance to adenine arabinoside in Toxoplasma gondii, and cloning of the adenosine kinase locus."
    Sullivan W.J. Jr., Chiang C.-W., Wilson C.M., Naguib F.N.M., el Kouni M.H., Donald R.G.K., Roos D.S.
    Mol. Biochem. Parasitol. 103:1-14(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: RH.
  2. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding."
    Schumacher M.A., Scott D.M., Mathews I.I., Ealick S.E., Roos D.S., Ullman B., Brennan R.G.
    J. Mol. Biol. 296:549-567(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS).
  3. "Crystal structure of adenosine kinase from Toxoplasma gondii at 1.8 A resolution."
    Cook W.J., DeLucas L.J., Chattopadhyay D.
    Protein Sci. 9:704-712(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiADK_TOXGO
AccessioniPrimary (citable) accession number: Q9TVW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3