Reviewed, UniProtKB/Swiss-Prot Q9TVW2 (ADK_TOXGO)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20
Alternative name(s):
    Adenosine 5'-phosphotransferase

Gene names

Name:

AK

Organism

Toxoplasma gondii

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP-dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. It is a key purine metabolic enzyme in the opportunistic parasitic protozoan toxoplasma gondii as it cannot synthesize purines de novo.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 363

363

Adenosine kinase

PRO_0000080056

Sites

Active site

318

1

Metal binding

185

1

Magnesium; via carbonyl oxygen

Metal binding

188

1

Magnesium; via carbonyl oxygen

Metal binding

191

1

Magnesium; via carbonyl oxygen

Secondary structure

1

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363

Helix Strand Turn

Details...

Beta strand

15 – 18

4

Beta strand

22 – 28

7

Helix

31 – 36

6

Beta strand

44 – 46

3

Helix

49 – 51

3

Helix

54 – 58

5

Helix

59 – 61

3

Beta strand

64 – 68

5

Helix

70 – 82

13

Beta strand

87 – 97

11

Helix

98 – 109

12

Beta strand

113 – 119

7

Beta strand

125 – 131

7

Beta strand

136 – 141

6

Helix

143 – 147

5

Helix

154 – 157

4

Turn

158 – 160

3

Beta strand

163 – 167

5

Helix

168 – 172

5

Helix

176 – 185

10

Beta strand

192 – 196

5

Helix

200 – 205

6

Helix

207 – 215

9

Beta strand

218 – 223

6

Helix

224 – 233

10

Helix

239 – 241

3

Helix

249 – 264

16

Beta strand

273 – 279

7

Beta strand

282 – 289

8

Beta strand

295 – 300

6

Helix

307 – 309

3

Helix

316 – 329

14

Helix

334 – 349

16

Beta strand

351 – 355

5

Sequences

	Sequence		Length	Mass (Da)
	Q9TVW2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6C621C7D5A56739C	FASTA	363	38,356
	10 20 30 40 50 60 MAVDSSNSAT GPMRVFAIGN PILDLVAEVP SSFLDEFFLK RGDATLATPE QMRIYSTLDQ 70 80 90 100 110 120 FNPTSLPGGS ALNSVRVVQK LLRKPGSAGY MGAIGDDPRG QVLKELCDKE GLATRFMVAP 130 140 150 160 170 180 GQSTGVCAVL INEKERTLCT HLGACGSFRL PEDWTTFASG ALIFYATAYT LTATPKNALE 190 200 210 220 230 240 VAGYAHGIPN AIFTLNLSAP FCVELYKDAM QSLLLHTNIL FGNEEEFAHL AKVHNLVAAE 250 260 270 280 290 300 KTALSTANKE HAVEVCTGAL RLLTAGQNTG ATKLVVMTRG HNPVIAAEQT ADGTVVVHEV 310 320 330 340 350 360 GVPVVAAEKI VDTNGAGDAF VGGFLYALSQ GKTVKQCIMC GNACAQDVIQ HVGFSLSFTS LPC

References

[1]	"Insertional tagging of at least two loci associated with resistance to adenine arabinoside in Toxoplasma gondii, and cloning of the adenosine kinase locus." Sullivan W.J. Jr., Chiang C.-W., Wilson C.M., Naguib F.N.M., el Kouni M.H., Donald R.G.K., Roos D.S. Mol. Biochem. Parasitol. 103:1-14(1999) [PubMed: 10514076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: RH.
[2]	"Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding." Schumacher M.A., Scott D.M., Mathews I.I., Ealick S.E., Roos D.S., Ullman B., Brennan R.G. J. Mol. Biol. 296:549-567(2000) [PubMed: 10669608] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS).
[3]	"Crystal structure of adenosine kinase from Toxoplasma gondii at 1.8 A resolution." Cook W.J., DeLucas L.J., Chattopadhyay D. Protein Sci. 9:704-712(2000) [PubMed: 10794412] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF128274 Genomic DNA. Translation: AAF01261.1.
AF128275 mRNA. Translation: AAF01262.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DGM	X-ray	1.80	A	1-363	[»]
1LII	X-ray	1.73	A	1-363	[»]
1LIJ	X-ray	1.86	A	1-363	[»]
1LIK	X-ray	2.55	A	1-363	[»]
1LIO	X-ray	2.50	A	1-363	[»]
2A9Y	X-ray	1.35	A	1-359	[»]
2A9Z	X-ray	1.35	A	1-359	[»]
2AA0	X-ray	1.75	A	1-359	[»]
2AB8	X-ray	1.75	A	1-359	[»]
2ABS	X-ray	1.10	A	1-359	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.7.1.20. 262552.

Family and domain databases

InterPro

IPR001805. Adenokinase.
IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]

PANTHER

PTHR10584:SF24. Adenokinase. 1 hit.

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PRINTS

PR00989. ADENOKINASE.

PROSITE

PS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADK_TOXGO

Accession

Primary (citable) accession number: Q9TVW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)