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Q9TVW2

- ADK_TOXGO

UniProt

Q9TVW2 - ADK_TOXGO

Protein

Adenosine kinase

Gene

AK

Organism
Toxoplasma gondii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    ATP-dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. It is a key purine metabolic enzyme in the opportunistic parasitic protozoan toxoplasma gondii as it cannot synthesize purines de novo.

    Catalytic activityi

    ATP + adenosine = ADP + AMP.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi185 – 1851Magnesium; via carbonyl oxygen
    Metal bindingi188 – 1881Magnesium; via carbonyl oxygen
    Metal bindingi191 – 1911Magnesium; via carbonyl oxygen
    Active sitei318 – 3181

    GO - Molecular functioni

    1. adenosine kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. phosphotransferase activity, alcohol group as acceptor Source: InterPro

    GO - Biological processi

    1. AMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00588; UER00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine kinase (EC:2.7.1.20)
    Short name:
    AK
    Alternative name(s):
    Adenosine 5'-phosphotransferase
    Gene namesi
    Name:AK
    OrganismiToxoplasma gondii
    Taxonomic identifieri5811 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363Adenosine kinasePRO_0000080056Add
    BLAST

    Interactioni

    Structurei

    Secondary structure

    1
    363
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi15 – 184
    Beta strandi22 – 287
    Helixi31 – 366
    Beta strandi44 – 463
    Helixi49 – 568
    Helixi58 – 614
    Beta strandi64 – 696
    Helixi70 – 8213
    Beta strandi87 – 948
    Helixi98 – 11013
    Beta strandi113 – 1186
    Beta strandi125 – 1328
    Beta strandi135 – 1417
    Helixi143 – 1475
    Helixi154 – 1574
    Turni158 – 1603
    Beta strandi163 – 1675
    Helixi168 – 1714
    Helixi176 – 18611
    Beta strandi188 – 1903
    Beta strandi192 – 1965
    Helixi200 – 2056
    Helixi207 – 2159
    Beta strandi218 – 2236
    Helixi224 – 23411
    Helixi239 – 2413
    Helixi249 – 26416
    Beta strandi274 – 2785
    Beta strandi284 – 2896
    Turni291 – 2933
    Beta strandi295 – 3006
    Helixi307 – 3093
    Helixi316 – 32914
    Helixi334 – 34916
    Beta strandi351 – 3533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DGMX-ray1.80A1-363[»]
    1LIIX-ray1.73A1-363[»]
    1LIJX-ray1.86A1-363[»]
    1LIKX-ray2.55A1-363[»]
    1LIOX-ray2.50A1-363[»]
    2A9YX-ray1.35A1-363[»]
    2A9ZX-ray1.35A1-363[»]
    2AA0X-ray1.75A1-363[»]
    2AB8X-ray1.75A1-363[»]
    2ABSX-ray1.10A1-363[»]
    ProteinModelPortaliQ9TVW2.
    SMRiQ9TVW2. Positions 10-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9TVW2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the carbohydrate kinase PfkB family.Curated

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    InterProiIPR001805. Adenokinase.
    IPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011611. PfkB_dom.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
    PfamiPF00294. PfkB. 1 hit.
    [Graphical view]
    PRINTSiPR00989. ADENOKINASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9TVW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVDSSNSAT GPMRVFAIGN PILDLVAEVP SSFLDEFFLK RGDATLATPE    50
    QMRIYSTLDQ FNPTSLPGGS ALNSVRVVQK LLRKPGSAGY MGAIGDDPRG 100
    QVLKELCDKE GLATRFMVAP GQSTGVCAVL INEKERTLCT HLGACGSFRL 150
    PEDWTTFASG ALIFYATAYT LTATPKNALE VAGYAHGIPN AIFTLNLSAP 200
    FCVELYKDAM QSLLLHTNIL FGNEEEFAHL AKVHNLVAAE KTALSTANKE 250
    HAVEVCTGAL RLLTAGQNTG ATKLVVMTRG HNPVIAAEQT ADGTVVVHEV 300
    GVPVVAAEKI VDTNGAGDAF VGGFLYALSQ GKTVKQCIMC GNACAQDVIQ 350
    HVGFSLSFTS LPC 363
    Length:363
    Mass (Da):38,356
    Last modified:May 1, 2000 - v1
    Checksum:i6C621C7D5A56739C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF128274 Genomic DNA. Translation: AAF01261.1.
    AF128275 mRNA. Translation: AAF01262.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF128274 Genomic DNA. Translation: AAF01261.1 .
    AF128275 mRNA. Translation: AAF01262.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DGM X-ray 1.80 A 1-363 [» ]
    1LII X-ray 1.73 A 1-363 [» ]
    1LIJ X-ray 1.86 A 1-363 [» ]
    1LIK X-ray 2.55 A 1-363 [» ]
    1LIO X-ray 2.50 A 1-363 [» ]
    2A9Y X-ray 1.35 A 1-363 [» ]
    2A9Z X-ray 1.35 A 1-363 [» ]
    2AA0 X-ray 1.75 A 1-363 [» ]
    2AB8 X-ray 1.75 A 1-363 [» ]
    2ABS X-ray 1.10 A 1-363 [» ]
    ProteinModelPortali Q9TVW2.
    SMRi Q9TVW2. Positions 10-359.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q9TVW2.
    ChEMBLi CHEMBL2982.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00588 ; UER00659 .

    Miscellaneous databases

    EvolutionaryTracei Q9TVW2.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    InterProi IPR001805. Adenokinase.
    IPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011611. PfkB_dom.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    PANTHERi PTHR10584:SF24. PTHR10584:SF24. 1 hit.
    Pfami PF00294. PfkB. 1 hit.
    [Graphical view ]
    PRINTSi PR00989. ADENOKINASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    PROSITEi PS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insertional tagging of at least two loci associated with resistance to adenine arabinoside in Toxoplasma gondii, and cloning of the adenosine kinase locus."
      Sullivan W.J. Jr., Chiang C.-W., Wilson C.M., Naguib F.N.M., el Kouni M.H., Donald R.G.K., Roos D.S.
      Mol. Biochem. Parasitol. 103:1-14(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: RH.
    2. "Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding."
      Schumacher M.A., Scott D.M., Mathews I.I., Ealick S.E., Roos D.S., Ullman B., Brennan R.G.
      J. Mol. Biol. 296:549-567(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS).
    3. "Crystal structure of adenosine kinase from Toxoplasma gondii at 1.8 A resolution."
      Cook W.J., DeLucas L.J., Chattopadhyay D.
      Protein Sci. 9:704-712(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

    Entry informationi

    Entry nameiADK_TOXGO
    AccessioniPrimary (citable) accession number: Q9TVW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3