Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription elongation factor SPT4

Gene

spt4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II. DSIF enhances transcriptional pausing at sites proximal to the promoter, which may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF may also promote transcriptional elongation within coding regions. DSIF is required for the transcriptional induction of heat shock response genes and regulation of genes which control anterior-posterior patterning during embryonic development (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 36C4-typeSequence analysisAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-112382 Formation of RNA Pol II elongation complex
R-DME-113418 Formation of the Early Elongation Complex
R-DME-674695 RNA Polymerase II Pre-transcription Events
R-DME-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-DME-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT4
Alternative name(s):
DRB sensitivity-inducing factor small subunit
Short name:
DSIF small subunit
dSpt4
Gene namesi
Name:spt4
ORF Names:CG12372
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0028683 spt4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103321 – 116Transcription elongation factor SPT4Add BLAST116

Proteomic databases

PaxDbiQ9TVQ5
PRIDEiQ9TVQ5

Expressioni

Gene expression databases

BgeeiFBgn0028683
ExpressionAtlasiQ9TVQ5 baseline and differential
GenevisibleiQ9TVQ5 DM

Interactioni

Subunit structurei

Interacts with Spt5 to form DSIF. DSIF interacts with TRX, RNA polymerase II and with the FACT complex, which is composed of DRE4/SPT16 and SSRP/SSRP1. DSIF can also interact with the exosome, a complex with 3'-5' exoribonuclease activity which is composed of at least Csl4, Dis3, Mtr3, Rrp4, Rrp6, Rrp40, Rrp42, Rrp46 and Ski6. DSIF may also interact with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of Cdk9 and cyclin-T (CycT).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Spt5Q9V4602EBI-165626,EBI-134850

GO - Molecular functioni

Protein-protein interaction databases

BioGridi62161, 4 interactors
IntActiQ9TVQ5, 1 interactor
STRINGi7227.FBpp0086953

Structurei

3D structure databases

ProteinModelPortaliQ9TVQ5
SMRiQ9TVQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 40Interaction with Spt5By similarityAdd BLAST40

Sequence similaritiesi

Belongs to the SPT4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 36C4-typeSequence analysisAdd BLAST21

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3490 Eukaryota
COG5204 LUCA
GeneTreeiENSGT00390000018559
InParanoidiQ9TVQ5
KOiK15171
OMAiVRGMYAV
OrthoDBiEOG091G0NIU
PhylomeDBiQ9TVQ5

Family and domain databases

CDDicd07973 Spt4, 1 hit
Gene3Di3.30.40.210, 1 hit
InterProiView protein in InterPro
IPR029040 RPABC4/Spt4
IPR009287 Spt4
IPR022800 Spt4/RpoE2_Znf
IPR038510 Spt4_sf
PANTHERiPTHR12882 PTHR12882, 1 hit
PfamiView protein in Pfam
PF06093 Spt4, 1 hit
PIRSFiPIRSF025023 Spt4, 1 hit
SMARTiView protein in SMART
SM01389 Spt4, 1 hit
SUPFAMiSSF63393 SSF63393, 1 hit

Sequencei

Sequence statusi: Complete.

Q9TVQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFDAIPKDL RGLRACLVCS LVKSFDQFET DGCENCEEFL RMKNNKDNVY
60 70 80 90 100
DHTSNNFDGI IALTTPTDSW VAKWQRLSRF TRGIYAISVS GTLPQSTLRD
110
MKNRGIVYKS RDRSQR
Length:116
Mass (Da):13,332
Last modified:May 1, 2000 - v1
Checksum:iA4EF4580DCAA8A57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF108353 mRNA Translation: AAF14223.1
AF109133 Genomic DNA Translation: AAF14224.1
AE013599 Genomic DNA Translation: AAF58482.2
AY075424 mRNA Translation: AAL68240.1
RefSeqiNP_610802.1, NM_136958.5
UniGeneiDm.19918

Genome annotation databases

EnsemblMetazoaiFBtr0087840; FBpp0086953; FBgn0028683
GeneIDi36387
KEGGidme:Dmel_CG12372
UCSCiCG12372-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiSPT4H_DROME
AccessioniPrimary (citable) accession number: Q9TVQ5
Secondary accession number(s): Q9V6E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health