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Q9TVL3 (CDK9_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cyclin-dependent kinase 9

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 9
Gene names
Name:cdk-9
ORF Names:H25P06.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) and spt-5. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Associates with cyclin-T (cit-1.1 or cit-1.2) to form P-TEFb By similarity.

Subcellular location

Nucleus Ref.2.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: Q9TVL3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Gene prediction based on EST data.
Isoform a (identifier: Q9TVL3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     432-445: NSQFEYTVGKGAHA → VRKFEAKWLKKREK
     446-478: Missing.
Note: Gene prediction based on EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Probable cyclin-dependent kinase 9
PRO_0000085807

Regions

Domain85 – 413329Protein kinase
Nucleotide binding91 – 999ATP By similarity

Sites

Active site2171Proton acceptor By similarity
Binding site1141ATP By similarity

Natural variations

Alternative sequence432 – 44514NSQFE…KGAHA → VRKFEAKWLKKREK in isoform a.
VSP_016291
Alternative sequence446 – 47833Missing in isoform a.
VSP_016292

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: C8E307EA3465E36B

FASTA47853,593
        10         20         30         40         50         60 
MSAQNYHAGL HQSSTQRPPK RPNTEHAQEP PKRALIGGQT TPSSSGGGQT PNGTNYELEV 

        70         80         90        100        110        120 
KQQLSNYQKL FDQATYPFIR DVSTYEKLNK IGQGTFGEVF KARCKNTGRM VALKKILMEN 

       130        140        150        160        170        180 
EKEGFPITAL REVKMLEQLK HPNITDLIEV CSAKSTGTTG SKDRATFYLV MALCAHDLAG 

       190        200        210        220        230        240 
LLSNPKIRMS LVHIKTMMKH LMSGLNKLHR SKILHRDMKA ANVLISKDGI LKLADFGLAR 

       250        260        270        280        290        300 
PFVQRENGAG PRPLYTNRVV TLWYRPPELL LGDRQYGTKI DVWGAGCIMA EMWTRQPIMQ 

       310        320        330        340        350        360 
GDTEQKQLQL ISGLCGSINK DVWPNCVNMP LWSAMSSEPN SALPQGKYRI LPNKMKNLMK 

       370        380        390        400        410        420 
FDAPDSKTDP FGKNVKQHDS ATDDDALHLL EILLAIDPDK RPTSDEAEDD IWFFKDPVPM 

       430        440        450        460        470 
ANVQDLMDTI PNSQFEYTVG KGAHANRGRH QNAQQRPNQQ QARPSNAIPA GQYRDTIF 

« Hide

Isoform a [UniParc].

Checksum: 2EB98637B821C984
Show »

FASTA44550,130

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo."
Shim E.Y., Walker A.K., Shi Y., Blackwell T.K.
Genes Dev. 16:2135-2146(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z92797, Z83118 Genomic DNA. Translation: CAB07237.2.
Z92797, Z83118 Genomic DNA. Translation: CAB07238.3.
PIRF87920.
T23123.
T23124.
RefSeqNP_492906.2. NM_060505.3.
NP_492907.2. NM_060506.3.
UniGeneCel.8202.

3D structure databases

ProteinModelPortalQ9TVL3.
SMRQ9TVL3. Positions 55-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid38431. 1 interaction.
IntActQ9TVL3. 2 interactions.
STRING6239.H25P06.2b.

Proteomic databases

PaxDbQ9TVL3.
PRIDEQ9TVL3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaH25P06.2b; H25P06.2b; H25P06.2. [Q9TVL3-1]
GeneID173023.
KEGGcel:CELE_H25P06.2.
UCSCH25P06.2b. c. elegans. [Q9TVL3-1]

Organism-specific databases

CTD173023.
WormBaseH25P06.2a; CE37838; WBGene00000410; cdk-9.
H25P06.2b; CE37839; WBGene00000410; cdk-9.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9TVL3.
KOK02211.
OMAHIKTMMK.
OrthoDBEOG76DTSM.
PhylomeDBQ9TVL3.

Enzyme and pathway databases

SignaLinkQ9TVL3.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio877955.
PROQ9TVL3.

Entry information

Entry nameCDK9_CAEEL
AccessionPrimary (citable) accession number: Q9TVL3
Secondary accession number(s): Q9XTH5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase